8results about How to "Reduce breeding workload" patented technology

The invention discloses an SNP molecular marker related to grass carp feed utilization rate and application thereof, and belongs to the technical field of aquatic animal molecular markers and aquatic genetic breeding. According to the invention, an SNP (Single Nucleotide Polymorphism) site obviously related to the feed utilization rate of the grass carp is screened out, the SNP molecular marker is positioned at the 51653399 site on the chromosome 2 of the grass carp, the polymorphic site of the SNP molecular marker is G / T, and when the genotype of the site is GG, the feed utilization rate of the grass carp is relatively high. The invention also discloses application of the SNP molecular marker, the amplification primer or the kit in breeding of grass carp with high feed utilization rate. The SNP molecular marker provided by the invention can be used for early breeding of grass carp with high feed utilization rate, greatly reduces the breeding workload, can significantly shorten the breeding time, accelerates the breeding process, improves the breeding efficiency, and reduces the breeding cost.

This invention relates to the field of agricultural technology, specifically providing a specific primer pair for identifying hybrid offspring of wild rice, cultivated rice, and cultivated rice and wild rice, and its application. Genomic DNA is extracted from rice leaves. Using this DNA as a template and the sequences corresponding to SEQ ID NO:3 and SEQ ID NO:4 as specific primers, PCR amplification is performed to obtain amplified fragments. After electrophoresis, fragments showing only a 146 bp major band are identified as wild rice, while those showing only a 155 bp major band are identified as cultivated rice. Fragments that simultaneously amplify both 155 bp and 146 bp fragments are identified as hybrid offspring of cultivated rice and wild rice. This invention can be applied to molecular marker detection and polymorphism detection in hybrid populations of wild rice and cultivated rice, facilitating rapid screening in distant hybridization breeding and improving breeding efficiency.

The invention discloses a Camel hoof valve gene ZbBCAT2 and application thereof to improvement of salt tolerance of plants. The CDS sequence of the Camel hoof valve ZbBCAT2 gene is as shown in SEQ ID NO.1, and the coded amino acid sequence is as shown in SEQ ID NO.2. The invention further discloses a preparation method of the Camel hoof valve gene ZbBCAT2. The invention also discloses an application of the Camel hoof valve gene ZbBCAT2 in improving the salt tolerance of plants. The overexpression of the Camel hoof valve ZbBCAT2 gene plays a negative regulation role in the salt tolerance. The Camel hoof valve ZbBCAT2 gene provided by the invention can be used as a candidate gene for breeding the salt-resistant new variety of the plant, and has important significance for cultivating the salt-resistant new variety of the plant by adopting a genetic engineering method, accelerating the breeding process and reducing the breeding workload.

This invention discloses genes nyxgjy1 This invention, applied to improving plant salt tolerance, falls under the field of molecular breeding technology. It creatively obtains a gene from moso bamboo, which is adapted to grow in slightly acidic soil, that enhances plant salt tolerance. nyxgjy1 Genes, genes nyxgjy1 The CDS sequence is shown in SEQ ID NO.3; the gene was expressed heterologously in rice. nyxgjy1 Overexpression was found nyxgjy1 Rice plants with this gene exhibit stronger salt tolerance, and the expression level is positively correlated with the plant's salt tolerance—the higher the expression level, the stronger the salt tolerance. nyxgjy1 Genes can be used for the selection of salt-tolerant plant varieties and the identification of salt tolerance, which is of great significance for shortening the breeding process, reducing the breeding workload, and accelerating the utilization / transformation of saline-alkali land.

The invention relates to the technical field of plant molecular biology, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker related to single-petal single-pistil or double-petal multi-pistil traits of plum blossom and application. The SNP molecular marker is closely linked with the characters of the petal number and the pistil number of the plum blossom at the same time, the single-petal single-pistil character and the double-petal multi-pistil character of the plum blossom can be identified in the early stage through the SNP molecular marker, and the single-petal single-pistil character and the double-petal multi-pistil variety plum blossom can be rapidly, accurately and efficiently identified in the seedling stage of the plum blossom. When being used for assisted breeding, the method can shorten the screening period of high-quality plum blossom and reduce the breeding workload, and has a wide application prospect.

The application discloses a SirB gene SNP molecular marker related to Dalbergia odorifera tree height and application thereof, and belongs to the technical field of genetic engineering. The SNP molecular marker disclosed by the application is located at the 290th base of the sequence shown in SEQ ID NO. 5, the polymorphism of the molecular marker is C or T, and the genotypes include CC, CT and TT. It is verified through correlation analysis that the tree height of the Dalbergia odorifera with the CC genotype is significantly higher than that of the individuals with the CT genotype and the TT genotype. The application further provides a kit for detecting the SNP molecular marker and application of the kit in the aspect of Dalbergia odorifera tree height trait auxiliary screening. With the aid of the molecular marker, the superior genotype individuals can be quickly and accurately identified at the Dalbergia odorifera seedling stage, the breeding efficiency of the Dalbergia odorifera can be significantly improved, and the application has important value for cultivating the Dalbergia odorifera with rapid growth.

The invention discloses an SNP molecular marker related to grass carp feed utilization rate and application thereof, and belongs to the technical field of aquatic animal molecular markers and aquatic genetic breeding. According to the invention, an SNP (Single Nucleotide Polymorphism) site obviously related to the feed utilization rate of the grass carp is screened out, the SNP molecular marker is positioned at the 51653399 site on the chromosome 2 of the grass carp, the polymorphic site of the SNP molecular marker is G / T, and when the genotype of the site is GG, the feed utilization rate of the grass carp is relatively high. The invention also discloses application of the SNP molecular marker, the amplification primer or the kit in breeding of grass carp with high feed utilization rate. The SNP molecular marker provided by the invention can be used for early breeding of grass carp with high feed utilization rate, greatly reduces the breeding workload, can significantly shorten the breeding time, accelerates the breeding process, improves the breeding efficiency, and reduces the breeding cost.

The application relates to the technical field of genetic engineering, and discloses application of a CsC3'H gene in increasing contents of citrus naringin, hesperidin and / or jaceosidin, a CDS sequence of the CsC3'H gene is shown as SEQ ID NO. 1, and a protein sequence coded by the CsC3'H gene is shown as SEQ ID NO. 2. It is found for the first time that the CsC3'H gene is positively correlated with the contents of citrus naringin, hesperidin and / or jaceosidin, and the highest content of citrus naringin, hesperidin and / or jaceosidin in a citrus transiently transformed with a CsC3'H gene overexpression vector can be 69.38% higher than that in a citrus transformed with a blank vector; therefore, the CsC3'H gene can be used as a candidate gene for breeding of a new citrus variety with high contents of citrus naringin, hesperidin and / or jaceosidin, and has important significance for cultivating a functional new citrus variety by using a genetic engineering method, accelerating a breeding process, reducing a breeding workload and improving comprehensive utilization of citrus.