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Brassica napus high oleic acid molecular marker and its preparation method and application

A technology of Brassica napus and molecular markers, which is applied in the direction of botanical equipment and methods, applications, DNA/RNA fragments, etc., can solve the problems of application limitations, achieve the effects of speeding up the process, shortening the breeding period, and reducing the breeding workload

Active Publication Date: 2012-02-08
HUAZHONG AGRI UNIV
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AI Technical Summary

Problems solved by technology

According to these QTLs, the SNP markers related to fad2 were designed, but there was only one 3' end difference in the primer design of the obtained SNP markers, and the effect in Brassica napus linolenic acid-assisted selection breeding was affected by the concentration of DNA template, primers Synthetic quality, primer concentration, PCR reaction system and parameters and other factors have greatly limited its application in actual production.
There is no report about the application of these markers in Brassica napus high oleic acid-assisted selection breeding, and there is no report on background selection in Brassica napus high-oleic acid breeding using molecular marker-assisted selection

Method used

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  • Brassica napus high oleic acid molecular marker and its preparation method and application
  • Brassica napus high oleic acid molecular marker and its preparation method and application
  • Brassica napus high oleic acid molecular marker and its preparation method and application

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Embodiment 1

[0040] 1. Establish double haploid (DH) population:

[0041] In this example, the high oleic acid strain A A254 was used as the female parent (the oleic acid content was 76.91%, and the seeds of this material were sent to the Chinese typical culture in Wuhan University, Wuhan City, Hubei Province on November 20, 2009. Preservation Center, its preservation number is CCTCC-P200909), common oleic acid content of rapeseed line A A177 (oleic acid content is 61.95%, the seeds of this material have been delivered to Wuhan University, Wuhan City, Hubei Province on November 20, 2009 F 1 , using F 1 The pollen of the plant is cultured through microspores (see the literature for microspore culture methods, Yu Fengqun, etc., some culture factors for improving the seedling rate of Brassica napus microspore embryoids, Acta Crops, 1997, 23 (2): 165- 168) 190 double haploid (DH for short) populations were obtained.

[0042] 2. Extraction of Brassica napus genomic DNA

[0043] Genomic DNA ...

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Abstract

The invention belongs to the technical field of rapeseed molecular breeding, and in particular relates to the preparation of a high-oleic-acid co-dominant SCAR molecular marker in Brassica napus and its application as marker-assisted selection in cultivating new varieties of Brassica napus high-oleic acid. The present invention identifies the difference between the nucleotide sequences of A254 and A177 by cloning and sequencing the fad2 gene DNA fragments of the high-oleic acid rapeseed rape line AA254 and the low-oleic acid rapeseed rape line AA177. The primer pair YQ-Fad2a-1 and YQ-Fad2a-2 were developed based on the differences in the provided nucleotide sequences, and a co-dominant SCAR molecular marker capable of distinguishing high oleic acid from low oleic acid in Brassica napus was obtained. The invention provides a new marker for rapeseed molecular breeding. The invention also discloses the method and application for preparing the molecular marker.

Description

technical field [0001] The invention belongs to the technical field of molecular breeding of rapeseed, and in particular relates to the preparation and application of a high oleic acid molecular marker of Brassica napus. The molecular markers can be used as marker-assisted selection for breeding of high oleic rapeseed rape, and provide new molecular markers for cultivating genetically stable new varieties of high oleic rapeseed. Background technique [0002] The composition of fatty acids in rapeseed directly determines the quality of rapeseed oil and its nutritional value to humans. Oleic acid is an 18-carbon monounsaturated fatty acid, and rapeseed oil with high oleic acid content has high application value in edible oil and industry. The increase of monounsaturated fatty acids and the decrease of polyunsaturated fatty acids can increase the oxidation stability of edible oils and reduce oxidation products. When frying food, high oleic acid edible oil does not smoke at hi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11C12P19/34A01H1/04
Inventor 周永明傅廷栋杨庆勇
Owner HUAZHONG AGRI UNIV
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