High-efficient breeding technique of Eurasian raisin grape

A seedless grape, technology, applied in horticulture, botanical equipment and methods, gardening tools/equipment, etc., can solve the problems of inability to achieve the purpose of cultivation, low breeding efficiency, low probability of seedless, and achieve convenient preparation and storage. , the effect of improving the development rate of young embryos and accelerating the breeding process

Active Publication Date: 2009-10-28
HANGZHOU BLUE SKY LANDSCAPE CONSTR GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] 2. The zygotic embryos of seedless grapes are aborted during the development process and cannot develop into normal seeds. Therefore, conventional breeding can only use seedless varieties as male parents to crossbreed seedless grape varieties, but the probability of seedless grapes in the offspring is low ( 0~15.9%), low breeding efficiency
In terms of the sampling time of immature embryos, if the collection time is too early, the immature embryos are too small to survive; if the collection time is too late, the immature embryos have already died, and the purpose of cultivation cannot be achieved.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1: A kind of high-efficiency breeding technology of Eurasian seedless grape, specifically comprising the following steps: (1) conventional pollination and fertilization during flowering, picking young fruit 30 to 50 days after flowering, washing with running water under running water for 1hr, ultra-clean work On the table, soak the young fruit in 70% alcohol for 1 minute, rinse with sterile water for 3 times; then soak in 0.1% mercuric chloride for 10 minutes, then rinse with sterile water for 4 to 5 times, (2) cut open under sterile conditions Ovules were removed from berries and inoculated on embryo culture medium containing different levels of plant growth regulators gibberellin acid (GA3), indole acetic acid (IAA), 6-benzylaminoadenine (6-BA) embryo rescue. (3) Conditions for embryo rescue: place the inoculated Erlenmeyer flask in a 5-8°C incubator for 60 days at low temperature, and then transfer it to a culture room for 30 days. The volume of embryos d...

example 1

[0025] Example 1: Seedless white grapes are emasculated and bagged three days before the first flowering period. Choose a sunny morning (from 6:00 to 7:00) when the temperature is 20-30°C and there is no wind. When emasculating, remove the corolla and stamens together with pointed tweezers. It is necessary to remove the stamens and avoid damaging the stigma. In order to prevent self-fertilization caused by pollen falling on the stigma during emasculation, the stigma is washed with water after each emasculation, and bagged immediately after 10 days. More than 100 grains per ear. 100 ears of each variety were emasculated. For each variety, at least 20 vigorous plants should be selected as female parents. Post-hybridization is managed using conventional techniques.

[0026] Pick the young fruit 30-50 days after flowering, wash it under running water for 1 hour, soak the young fruit in 70% alcohol for 1 minute under aseptic conditions on the ultra-clean workbench, and rinse i...

example 2

[0032]Example 2: Excellent seedless grapes (super seedless, yellow grape) are emasculated and bagged three days before the first flowering period. Choose a sunny morning (from 6:00 to 7:00) when the temperature is 20-30°C and there is no wind. When emasculating, remove the corolla and stamens together with pointed tweezers. It is necessary to remove the stamens and avoid damaging the stigma. In order to prevent self-fertilization caused by pollen falling on the stigma during emasculation, the stigma is washed with water after each emasculation, and bagged immediately after drying. More than 100 grains per ear. 100 ears of each variety were emasculated. For each variety, at least 20 vigorous plants should be selected as female parents. Post-hybridization is managed using conventional techniques.

[0033] Pick the young fruit 30-50 days after flowering, wash it under running water for 1 hour, soak the young fruit in 70% alcohol for 1 minute under aseptic conditions on the u...

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PUM

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Abstract

The present invention relates to a high-efficient breeding technique of Eurasian raisin grape. After the raisin grape has flowered for 30-50d, the young fruit of the grape is picked. The ovule is taken out in a sterilized state. The embryo is inoculated to an MS culture medium containing different levels of plant growth regulators of gibberellic acid (GA3), indoleacetic acid (IAA) and 6-benzylaminopurine (6-BA) for executing embryo rescue. The average embryo development rate using the breeding technique of the invention is increased from 8.3-11.9% of a patient disclosed in the background art to 84%. The germination rate obtains 75.33% and the seedling rate obtains 74.17%. Furthermore the embryo rescue technique of the raisin grape obtained through the technique of the invention has the advantages of increased seedling rate of offspring, easier obtainment of more filial generation, increased parent selection range of raisin grape cross breeding, accelerated breeding process of new species of the raisin grape and increased breeding efficiency of the raisin grape through increasing the embryo development rate and the germination rate of the raisin grape.

Description

technical field [0001] The invention relates to a high-efficiency breeding technology for Eurasian seedless grapes, belonging to the technical field of plant breeding. Background technique [0002] 1. Publication No. CN 101238791 A. Title "A Technology for Improving Seedless Grape Breeding Efficiency", which is prepared by dissolving a plant growth inhibitor substance chlormequat or a cytokinin substance 6-BA The medicament is sprayed on the flower spikes 10-20 days before flowering, the grape fruit grains are picked 40-50 days after flowering, and the ovules are taken out for embryo rescue. The embryo development rate of the seedless white of the present invention is reduced from 0 to 4.4%. Increased to 8.3-11.9%; Moritani Seedless increased from 0.5-2.5% to 1.8-21.9%; Flame Seedless increased from 7.1-17.1% to 14.1-25.4%. [0003] 2. The zygotic embryos of seedless grapes are aborted during the development process and cannot develop into normal seeds. Therefore, conventio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 刘海琳
Owner HANGZHOU BLUE SKY LANDSCAPE CONSTR GROUP
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