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Method for breeding rape self-incompatible line assisted by S haplotype molecular markers

A molecular marker-assisted and molecular marker technology, which is applied in botany equipment and methods, biochemical equipment and methods, horticultural methods, etc., can solve the problem of inability to accurately judge affinity/incompatibility, inability to identify plant phenotype, Problems such as short detection time can achieve the effect of reducing breeding workload, accurate judgment, and promoting rapid breeding

Inactive Publication Date: 2019-04-05
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method can identify the compatibility of plants at the flowering stage, but it is difficult to apply it to field breeding: first, it cannot accurately judge the compatibility / incompatibility, and some plants do not elongate their pollen tubes at the early flowering stage and show strong self-indulgence. Cross-incompatibility, self-incompatibility disappears in late flowering stage, and self-fertilization can still set seeds normally; second, it needs to be carried out in the flowering stage, and the detection time is extremely short, so it is impossible to identify the phenotype of a large number of plants

Method used

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  • Method for breeding rape self-incompatible line assisted by S haplotype molecular markers
  • Method for breeding rape self-incompatible line assisted by S haplotype molecular markers
  • Method for breeding rape self-incompatible line assisted by S haplotype molecular markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] 1), F 1 Obtaining the seeds:

[0065] In the spring of 2010, in the experimental farm of Huazhong Agricultural University, the self-incompatibility line "S-1300" of Brassica napus was used as the female parent, and it was crossed with the conventional variety "Zhongshuang 11" to obtain the first generation hybrid (F 1 )seed. "Zhongshuang 11" is a rapeseed variety selected by the Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences (Zhongshuang 9 / 2F10) / / 26102. It was approved by the National Crop Variety Approval Committee on December 2, 2008. The approval number It is the national inspection oil 2008030.

[0066] 2) The S haplotype of the inbred line "Zhongshuang 11"

[0067] In the autumn of 2010, "S-1300", "Zhongshuang 11" and F 1 Each seed is planted in 3 rows, and the plant and row spacing are 15cm and 30cm. At the 3-leaf stage, "S-1300", "Zhongshuang 11" and F 1 Heart leaves of 5 plants each were mixed, and DNA was extracted and purifi...

Embodiment 2

[0101] 1), F 1 acquisition of seeds

[0102] In the spring of 2011, in the experimental farm of Huazhong Agricultural University, the self-incompatibility line "S-1300" of Brassica napus was used as the female parent, and the conventional variety "Zheyou 50" was used as the male parent to obtain the first generation hybrid (F 1 )seed. "Zheyou 50" is a rapeseed variety bred by Huyou 15 / Zheshuang 6 by the Institute of Crop and Nuclear Technology Utilization of Zhejiang Academy of Agricultural Sciences. It was approved by the Second National Crop Variety Approval Committee on November 18, 2011 It was approved by the Sixth Meeting, and the approval number is National Examination Oil 2011013.

[0103] 2) The S haplotype of the inbred line "Zheyou 50"

[0104] In the autumn of 2011, "S-1300", "Zheyou 50" and F 1 Seeds were planted in 2 rows, 10 plants in each row, with a plant-to-plant spacing of 15 cm and a row spacing of 30 cm. At the 3-leaf stage, "S-1300", "Zheyou 50" and F...

Embodiment 3

[0116] 1) Identification of the S haplotype of the inbred line "Huashuang 5"

[0117] In the spring of 2009, the self-incompatibility line "S-1300" of Brassica napus was used as the female parent and the conventional variety "Huashuang 5" was used as the male parent in the experimental farm of Huazhong Agricultural University to obtain the first generation hybrid (F 1 )seed. "Huashuang 5" is a high-yielding double-low rapeseed that was bred by Huazhong Agricultural University using Zhongyou 821 / / Huayou 3 / RS-1 double-low selection line x Huashuang 3 / 3 / Zhongshuang 4 The variety was approved by the National Crop Variety Approval Committee in 2004, and the approval number is: National Examination Oil 2004006.

[0118] In the autumn of 2009, "S-1300", "Huashuang 5" and F 1 Seeds were planted in 2 rows, 10 plants in each row, with a plant-to-plant spacing of 15 cm and a row spacing of 30 cm. At the seedling stage, "S-1300", "Huashuang 5" and F 1 Heart leaves of 5 plants each wer...

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Abstract

The invention belongs to the technical field of rape molecular breeding, and discloses a method for breeding a rape self-incompatible line assisted by an S haplotype molecular marker, which comprisesthe following steps of: identifying an S haplotype of inbred line, double haploids of the self-incompatible line and inbred line, F2 populations of the self-incompatible line and inbred line, backcross populations of the self-incompatible line and inbred line and an S haplotype of the offspring by utilizing a molecular marker technology, the self-incompatible line of cabbage type rape is obtained,the method can overcome the defect of selection according to the self-incompatible phenotype in the rape breeding process, the breeding workload is greatly reduced, and the rapid cultivation of the self-incompatible line of the cabbage type rape is achieved, meanwhile, the S haplotype is detected by utilizing multiple PCR molecular markers, the error judgment on the result caused by the error ofthe experimental operation process is overcome, and the S haplotype of a plant can be accurately judged.

Description

technical field [0001] The invention belongs to the technical field of rapeseed molecular breeding, and in particular relates to a method for assisting self-incompatibility breeding of rapeseed with S haplotype molecular markers; specifically designing a self-incompatibility S haplotype molecule artificially created Method for Breeding Brassica napus Self-incompatibility Lines by Combining Detection Technology with Hybridization, Backcrossing and Microspore Culture Technology Background technique [0002] Breeding hybrids is an important way to improve Brassica napus (also known as rape). Self-incompatibility is one of the ways to utilize heterosis in rapeseed. Fu Tingdong et al. created self-incompatibility Brassica napus by crossing Brassica napus and Chinese cabbage, and proposed a self-incompatibility "three-line" (self-incompatibility line, maintainer line, restorer line) hybrid breeding method . Based on the development of a method for mass propagation of self-incom...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04A01H4/00A01H6/20C12Q1/6895
CPCA01H1/02A01H1/04A01H4/008C12Q1/6895C12Q2600/13
Inventor 马朝芝易斌文静傅廷栋
Owner HUAZHONG AGRI UNIV
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