Brassica napus L. grain weight major QTLs molecular marker and application thereof

A Brassica napus and molecular marker technology, applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of few reports on gene cloning and analysis related to grain weight, limited number of SSR markers, no Problems such as main effect QTLs, to achieve the effect of shortening the breeding period, speeding up the process, and reducing the breeding workload

Inactive Publication Date: 2011-04-20
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, due to the limited number of SSR markers, it is still difficult to conduct horizontal comparisons between different populations in QTL mapping studies.
[0006] At present, there are no reports on major QTLs related to grain weight that can be stably detected in different genetic backgrounds in rapeseed, and there are very few reports on the cloning and analysis of genes related to grain weight

Method used

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  • Brassica napus L. grain weight major QTLs molecular marker and application thereof
  • Brassica napus L. grain weight major QTLs molecular marker and application thereof
  • Brassica napus L. grain weight major QTLs molecular marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Acquisition of site-specific molecular markers for major QTLs for grain weight in Brassica napus

[0033] (1) Construction of DH population of Brassica napus A254 / A177 grain relocation population, field experiment and 1000-grain weight analysis. line) as the male parent to obtain F1, plant F1, take flower buds from the F1 plant and carry out microspore culture to obtain double haploid (DH) segregation population, a total of 238 lines of DH lines were obtained, and 190 lines were randomly selected for use. Construction of genome-wide genetic linkage map and mapping of grain weight QTL.

[0034] The DH line obtained above and its parents A254 / A177 and F1 were planted in the field in 2007-2008 and 2008-2009. The field experiment adopted a completely randomized block design, repeated three times, and each line planted two rows. There are 11-12 individual plants in each row, the average distance between plants is about 24cm, and the distance between rows is 30cm....

Embodiment 2

[0045] Example 2: Verification of the validity of the locus-specific markers of the main QTLs for grain weight in Brassica napus

[0046] (1) Validation of locus-specific markers for major QTLs for grain weight in Brassica napus

[0047] In order to detect the main effect of the two markers I0509 and J0609 on phenotypic variation, in the DH population, each line was grouped by the genotypes of these two loci, and the average value of thousand-grain weight was calculated. For the I0509 locus, in the AA genotype (allele locus from A254) group, the number of alleles containing positive additive effects from A254 in two years was significantly higher than that from A177 is higher. There is the same trend for J0609 (Table 3). It can be seen from the results in the table that the two sites located at A7 in Brassica napus are the main factors determining the grain weight.

[0048] (2) Verification of the combined effects of QTLs for grain weight in Brassica napus

[0049] The com...

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Abstract

The invention belongs to the technical field of rape molecular breeding and preparation of molecular markers, and particularly relates to preparation of a brassica napus L. grain weight major QTLs locus specific molecular marker. The marker can be used for auxiliary selection of molecular markers, fine positioning of grain weight trait loci and based cloning in the improvement on brassica napus L. grain weight traits. The marker is characterized in that double haploid (DH) is constructed by hybridizing brassica napus L. first A254 (large-grain pure line materials) as a female parent and brassica napus L. first A177 (small-grain pure line materials) as a male parent, and the molecular marker which is tightly linked with the grain weight major QTLs is obtained by analyzing DH group genotypes and thousand grain weight data and is named I0509 and J0609, and is relatively verified and applied. A new genetic marker is provided for molecular breeding of rape grain weight and also useful information is provided for fine positioning of thousand grain weight trait loci of the brassica napus L. and based cloning of relative genes.

Description

technical field [0001] The invention belongs to the fields of molecular breeding and biotechnology of rapeseed, and specifically relates to the discovery and separation of QTL related to grain weight of Brassica napus, and the development and application of related molecular markers. Background technique [0002] Brassica napus L. (hereinafter referred to as rapeseed) is one of the most important oil crops in the world. The seeds of rapeseed are not only the storage organs of oil and protein, but also the organs of plant life cycle continuation. Seed size or weight are very important economical traits. First of all, grain weight is one of the three major factors constituting plant yield per plant (effective silique number per plant, number of silique grains per plant, and grain weight), and therefore also determines yield (Clarke and Simpson, 1978; Butruille et al., 1999; Shi et al., 2009); secondly, seed size is also related to oil and protein content (Morgan et al., 1998...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11C12N15/10
Inventor 周永明傅廷栋范楚川蔡光勤
Owner HUAZHONG AGRI UNIV
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