The invention provides new genetic engineering bacteria used for producing beta-alanine, having strong synthetic ability and having higher enzyme activity, construction, expression and purification of the high-production engineering bacteria, and methods for synthesis of beta-alanine respectively through whole cell transformation and fermentation liquid direct transformation. A synthetic method comprises the steps: an L-aspartic-alpha-alanine decarboxylase (PanD) gene of lactobacillus plantarum is obtained by a gene engineering method and has the gene sequence number of NC-004567.2, the gene is constructed into a high-efficiency expression vector, then the high-efficiency expression vector is transformed into recipient bacteria, and thus the genetic engineering bacteria for producing beta-alanine are obtained. The genetic engineering bacteria are subjected to fermentation culture, 80 g/L of a substrate can be transformed by whole cells, and the beta-alanine content reaches 59.7 g/L; and the substrate is directly added into a fermentation liquid, the transformation concentration can reach 10 g/L, the beta-alanine content reaches 6.8 g/L, and the synthetic ability is higher than that of conventional reported genetic engineering bacteria.