122 results about "New genetics" patented technology

The invention belongs to the field of plant genetic engineering, and discloses application of a vitis amurensis cold-resistant gene VaWRKY65 in plant cold-resistant genetic improvement, the VaWRKY65 gene is a transcription factor separated and cloned from extremely cold-resistant vitis amurensis, and the sequence of the VaWRKY65 gene is shown as SEQ ID NO.1. The invention further discloses a preparation method of the vitis amurensis cold-resistant gene VaWRKY65. The gene is used for constructing an overexpression and interference vector, the overexpression and interference vector is respectively introduced into arabidopsis thaliana and vitis amurensis through agrobacterium tumefaciens-mediated genetic transformation, and biological function verification of the obtained transgenic plant shows that the cloned VaWRKY65 gene has the function of improving the cold resistance of the plant. The discovery of the gene provides a new gene resource for plant stress resistance molecular design breeding and provides a new genetic resource for implementation of green agriculture and water-saving agriculture, and development and utilization of the genetic resource are beneficial to reduction of agricultural production cost and realization of environmental friendliness.

The invention relates to the technical field of gene engineering, and discloses an METS2 gene for regulating and controlling the grain shape of rice, a protein and application of the METS2 gene, and the nucleotide sequence of the METS2 gene is shown as SEQ ID NO.1. The invention further discloses application of the METS2 gene in regulation and control of the rice grain shape. According to the METS2 gene for regulating and controlling the rice grain shape, the protein and the application of the METS2 gene, the METS2 gene is overexpressed in a rice plant, short-grain-shaped rice can be obtained, the short-grain-shaped rice is not prone to breakage in the processing process, and the quality of a finished product is high; a basis is provided for further clarification of the molecular mechanism of the rice grain shape, and new gene resources and breeding resources are provided for rice breeding.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a wheat transcription factor TaWRKY22 and a coding gene thereof in regulation and control of plant stripe rust resistance. The function of the wheat transcription factor TaWRKY22 is researched through molecular biology and genetics methods, it is found that knockout of the TaWRKY22 gene significantly improves the resistance of wheat to stripe rust, overexpression of the TaWRKY22 gene reduces the resistance of wheat to stripe rust, and then it is indicated that the wheat transcription factor TaWRKY22 plays a negative regulation role in wheat stripe rust resistance. Therefore, the TaWRKY22 gene is subjected to targeted modification through a gene editing technology, a new genetic material and a molecular breeding strategy can be provided for wheat stripe rust resistance breeding, and an important material and technical support are provided for breeding of wheat disease-resistant varieties.

The invention discloses a method for obtaining leymus chinensis mutants by using heavy ion beams to irradiate seed calluses, relates to the technical field of leymus chinensis breeding biology, and solves the three-low problems of low heading rate, low maturing rate and low germination rate of leymus chinensis seeds. After the leymus chinensis callus is subjected to induction culture, selecting faint yellow granular callus obtained by induction culture, and transferring the faint yellow granular callus into a culture dish filled with an induction culture medium for subculture; carrying out irradiation treatment by using a 5Gy carbon ion beam, transferring the callus subjected to irradiation treatment into a sterile culture bottle filled with a differential culture medium, and culturing to obtain adventitious buds; cutting off callus adhered to the base under a sterile condition, and inserting into a rooting culture medium for rooting culture after a rooting point is exposed; and after obvious root systems grow out, carrying out potting culture to obtain the leymus chinensis mutant. According to the invention, new genetic variation can be created, early in-vitro selection is realized, more effective mutants are obtained as new germplasm resources of leymus chinensis, and the breeding process of leymus chinensis is accelerated.

The invention belongs to the technical field of biology, and particularly relates to a TaGAT2-1B gene resistant to wheat stripe rust and application of the TaGAT2-1B gene, and the nucleotide sequence of the TaGAT2-1B gene is shown as SEQ ID NO.3. It is found that the TaGAT2-1B gene and the encoding protein thereof can effectively improve the wheat stripe rust resistance, the TaGAT2-1B gene is a new stripe rust resistance gene in the wheat adult-plant stage, infection of various stripe rust strains can be effectively resisted, the field phenotype of the TaGAT2-1B gene shows the characteristic of slow rust, and the TaGAT2-1B gene and the encoding protein thereof have good application prospects. The discovery of the gene provides a new genetic resource for the research of the wheat stripe rust resistance mechanism and the wheat stripe rust resistance breeding.

The invention relates to the technical field of plant molecular genetic engineering, in particular to an FvemiR172c gene for regulating and controlling anthocyanin accumulation of strawberry fruits and application of the FvemiR172c gene. The nucleotide sequence of the FvemiR172c gene is as shown in SEQ ID NO. 1 (sequence identifier number 1). The invention discloses a nucleotide sequence of an FvemiR172c gene for regulating and controlling the anthocyanin content of strawberry fruits, and experiments show that the FvemiR172c gene or an expression vector containing the FvemiR172c gene can be applied to regulation and control of the anthocyanin content of the strawberry fruits so as to regulate and control the color of the strawberry fruits. The invention provides a new gene resource for creating different colors of strawberries and molecular breeding for promoting / inhibiting anthocyanin accumulation.

The invention provides application of a poplar PtoHsfB1 gene in plant growth, and belongs to the technical field of gene engineering. The function of promoting plant growth of the populus tomentosa gene PtoHsfB1 is found for the first time. Experimental results show that the expression quantity of the PtoHsfB1 gene in the transgenic populus tomentosa is greatly improved compared with that of non-transgenic populus tomentosa, the height, dry weight and fresh weight of a transgenic plant are higher than those of a non-transgenic plant, ABA synthesis and signal transduction can be reduced through overexpression of the gene PtoHsfB1, growth and development of the populus tomentosa are promoted, and the yield of the populus tomentosa is increased. Therefore, a new genetic resource is provided for improvement and molecular breeding of the poplar and other woody plants.

The invention provides an application of a citrus transcription factor CsAGL9 gene and a protein coded by the citrus transcription factor CsAGL9 gene in regulating and controlling the content of citric acid in citrus fruits, the nucleotide sequence of the CsAGL9 gene is shown as SEQ ID NO.1, and the amino acid sequence of the protein is shown as SEQ ID NO.2. The content of citric acid in the fruits can be reduced through overexpression in the oranges; the content of citric acid in fruits can be increased by silencing the gene. It is further verified that the gene promotes the activity of the citrus citric acid transporter CsTCT1. The discovery provides a new gene resource for citrus quality improvement and molecular design breeding, and provides an efficient tool for fruit quality improvement.

The invention belongs to the technical field of biology, and particularly relates to application of a GmNIC1 gene and a GmNIC2 gene to regulation and control of soybean cyst nematode resistance of plants, and the nucleotide sequence of the GmNIC1 gene is shown as SEQ ID NO.1; the nucleotide sequence of the GmNIC2 gene is as shown in SEQ ID NO. 2. The invention discloses the negative regulation effect of GmNIC1 and GmNIC2 genes in the resistance of soybean to cyst nematode (SCN) for the first time, and by constructing gene silencing mutants of GmNIC1 and GmNIC2, it is verified that the resistance of soybean to SCN can be remarkably enhanced due to function deficiency of the gene family. The application provides a new target for deep understanding of a soybean-nematode interaction mechanism, and provides a brand new gene resource and technical path for molecular design breeding of resistant soybeans.

The invention discloses a chloroplast RNA edited gene OsPPR7 for temperature-sensitive regulation and control of rice and application of the gene OsPPR7, and belongs to the technical field of gene engineering. The CDS sequence of the OsPPR7 gene is as shown in SEQ ID NO. 1. The OsPPR7 is an RNA (Ribonucleic Acid) editing factor which has a bidirectional regulation and control function and is sensitive to temperature. Editing of multiple sites on the rpoB gene is negatively regulated and controlled, and meanwhile RNA editing of multiple genes such as ndhB and ndhD which are crucial to the chloroplast function is positively regulated and controlled under high-temperature stress. Under the stress of high temperature of 35 DEG C, the loss of functions can lead to obvious reduction of RNA editing efficiency of a plurality of ndh genes, and serious yellowing of seedlings is caused. The key function of the rice OsPPR7 gene in regulation and control of rice growth and development and high-temperature response is clarified for the first time, the importance of the OsPPR7 gene in maintenance of chloroplast functions under high-temperature stress is clarified, and a new gene resource and a technical path are provided for cultivation of new varieties of high-temperature-resistant rice.

The invention belongs to the technical field of plant molecular genetic engineering, and particularly relates to a FaAL6 gene related to strawberry fruit hardness and application of the FaAL6 gene. The nucleotide sequence of the FaAL6 gene is as shown in SEQ ID NO. 1. The invention discloses a nucleotide sequence of a FaAL6 gene for regulating and controlling the hardness of strawberry fruits and an amino acid sequence coded by the FaAL6 gene, and experiments show that the FaAL6 gene or a construct or a host cell containing the FaAL6 gene can be applied to regulating and controlling the hardness of the strawberry fruits. The invention provides a new gene resource for molecular breeding for delaying strawberry fruit softening and improving strawberry fruit storage and transportation performance.

The invention belongs to the technical field of molecular biotechnology and molecular markers, and discloses a pig nipple number character related SNP molecular marker and application thereof, the site of the SNP molecular marker corresponds to the nucleic acid site at the 103001352bp site of the No.7 chromosome of the international pig reference genome Sscrofa11.1 version sequence, and the site is Ggt; and A basic group mutation. The SNP molecular marker associated with the nipple number character of the large white pig can be applied to genetic improvement of the growth and reproduction character of the large white pig, provides a new genetic marker resource for molecular marker-assisted selection of the nipple number character of the large white pig, can be applied to breeding of sows with high lactation capacity and high piglet survival rate, accelerates the breeding process, and improves the breeding efficiency. The reproductive performance of offspring large white pigs is improved.

The invention discloses a gene combination for risk prediction of acute graft versus host disease and donor screening before hematopoietic cell transplantation and application of the gene combination. According to the present invention, the gene combination with the post-transplantation aGVHD occurrence prediction and diagnosis value is found through the whole genome sequencing and the target gene capture sequencing method; on one hand, comprehensive judgment and evaluation are carried out by detecting gene combination mutation conditions of donors and recipients, selection of transplantation donors is optimized, and the survival rate and the survival quality of patients are improved; on the other hand, the occurrence of aGVHD after transplantation of the recipient can be predicted by detecting the gene combination mutation condition of the donor and the recipient, and a basis is provided for potential aGVHD diagnosis; and thirdly, a new genetic basis is provided for revealing the pathogenesis of the aGVHD, and a new drug target is provided for treating the aGVHD.

The invention belongs to the technical field of plant genetic engineering, and particularly discloses application of an important member of a potato mitogen-activated protein kinase (MAPK) family, namely an StMKK9 gene in regulation and control of maturity and cold resistance of potatoes. CDNA of a cultivar Desiree leaf is used as a template, the potato StMKK9 gene is separated and cloned, an over-expression vector is constructed, and the over-expression vector is used for expression of the potato. Genetic transformation of the potatoes is further carried out, it is found that the overexpressed StMKK9 gene can promote prematurity of the potatoes, meanwhile, the cold resistance of potato plants can be improved, and a new genetic material and a theoretical basis are provided for analysis of a potato tuberization molecular mechanism and improved breeding of the potatoes.

The invention discloses a molecular marker related to the sucrose content of peanut seed kernels, a KASP primer group and application of the molecular marker and the KASP primer group, and belongs to the technical field of molecular genetic breeding. The molecular marker disclosed by the invention is positioned at 108307340bp of a B03 chromosome, and the polymorphism of the molecular marker is G / A. The molecular marker related to the sucrose content of the peanut seed kernels is provided, a KASP primer group and a KASP analysis technology are developed according to the molecular marker, and the molecular marker can be used for detecting the sucrose content of the peanut seed kernels. When the molecular marker and the KASP primer group thereof are used for early identification and genetic breeding or genetic resource improvement of peanut strains, the peanut breeding period can be shortened, the genetic resource improvement process is accelerated, and new genetic resources and theoretical basis are provided for molecular assisted breeding.

The invention discloses an application of an overexpressed CrOASTL1 gene in improving the soluble protein content of leguminous forage, and belongs to the technical field of biology. 1, and the CDS sequence of the CrOASTL1 gene is as shown in SEQ ID NO. 2. The method comprises the following steps: cloning a CrOASTL1 gene segment, constructing a plant overexpression vector, carrying out plant transgenosis operation, and introducing into leguminous forage plant cells to obtain a leguminous forage transgenosis line with the overexpressed CrOASTL1 gene. The CrOASTL1 gene is over-expressed in medicago sativa, and the soluble protein content of leaves of the medicago sativa is remarkably increased by about 19%. The CrOASTL1 gene of chlamydomonas reinhardtii is applied to quality improvement of leguminous forage for the first time, the technical obstacle that the genetic relationship of species is relatively far is overcome, a brand new gene resource and an efficient technical approach are provided for cultivating a new variety of leguminous forage with high protein and high nutritional value, and the method has important significance in promoting sustainable development of grass and animal husbandry.

The present application relates to a kind of genes for regulating rice grain shape and its application, it is related to the field of plant genetic engineering.A kind of genes for regulating rice grain shape, the gene is CRC gene, sequence as shown in SEQ ID NO:1.The gene is obtained by reverse genetics method, can be applied to regulate rice grain shape, growth period, not only enriches the molecular mechanism of rice grain shape regulation, also provides new genetic resources for the study of rice grain shape improvement, provides new material selection for rice design breeding, in rice knockout the gene can cultivate small grain shape rice variety, provides new selection for the improvement of rice grain shape.

The invention discloses a gene OsNST2 for transporting nucleotide sugar and application of the gene OsNST2. A nucleotide sequence of a coding region of the gene is as shown in SEQ ID No. 1, and an amino acid sequence coded by the gene is as shown in SEQ ID No. 2. It is found for the first time that the growth, development and yield of rice are affected by the OsNST2 by regulating and controlling formation of rice cell walls, an OsNST2 gene knock-down strain is created through an RNAi technology, an OsNST2 gene overexpression strain is obtained through an overexpression transgenic technology, it is verified that the OsNST2 gene is a new gene for regulating and controlling formation of the rice cell walls, and a new gene resource is provided for high yield of rice.

The invention relates to the technical field of plant genetic engineering, in particular to application of a rice OsERF141 protein and a coding gene thereof in improving low-temperature tolerance of plants. Experiments show that the low-temperature resistance of an OsERF141 function deletion mutant strain is remarkably reduced, and the low-temperature resistance of an overexpressed OsERF141 transgenic strain is remarkably improved. The invention provides a new gene resource for the cold resistance problem of rice, and has important significance for the molecular mechanism research of low-temperature stress and the cultivation of new low-temperature-resistant varieties.

The invention relates to the technical field of gene engineering, in particular to a gene vector based on CsCMV mediation and a preparation method and application thereof. On the basis of a pCsCMV-CM vector, a Bsm BI site is successfully inserted into a non-coding region (UTR) behind a termination codon (TAG) at a CsCMV CP 3'end through an In-Fusion seamless cloning method, an infectious clone pCsCMV-Bs vector based on a Golden gate Coning strategy is constructed, a target gene segment can be rapidly cloned into a virus genome with high throughput, and the target gene segment can be cloned into the virus genome. The method is applied to cassava gene silencing and gene editing, the stability is higher, the phenotype duration time is longer, and a new genetic tool is provided for rapid and efficient gene function verification and directional character improvement of cassava.

The application discloses a corn kernel size related protein SSRP1 and application thereof. The technical problem solved is how to regulate the size of corn kernels. Specifically disclosed is the application of a protein, a substance for regulating the expression of a coding gene of the protein, or a substance for regulating the activity or content of the protein in regulating the size of plant kernels and / or preparing a product for regulating the size of plant kernels and / or plant breeding and / or preparing a plant breeding product, the protein being a protein as shown in sequence 3 in the sequence listing. The application provides a new genetic resource for the study of the size of plant kernels, in particular corn kernels, and will play an important role in the application in the field of corn seed production and breeding.

The invention relates to the field of plant genetic engineering, in particular to novel application of a soybean ACR gene family member GmACR39 in improving saline-alkaline tolerance of plants. The invention provides application of a separated gene in preparation of a product for improving saline-alkaline tolerance of plants, and the gene encodes a nucleotide sequence shown in SEQ ID NO: 1 or encodes a protein which has at least 80% identity with SEQ ID NO: 2 and has a function of improving saline-alkaline tolerance of plants. The expression of the gene is up-regulated under saline-alkaline stress, and the saline-alkaline tolerance of a transgenic plant can be remarkably improved by over-expression of the gene. The invention also provides an expression vector containing the gene, an sgRNA molecule targeting a promoter of the gene, a CRISPR activation system, and related reagents, kits, a method for cultivating saline-alkaline tolerant plants and detection means. The invention provides a new gene resource and a molecular breeding tool for cultivating new varieties of saline-alkaline tolerant crops.

The invention belongs to the technical field of crop breeding, and relates to application of a rice gene OsPOK1 to regulation and control of a rice grain shape and a mutant of the rice gene OsPOK1. The coding sequence of the rice gene OsPOK1 is as shown in SEQ ID NO. 1, and the amino acid sequence of the protein coded by the rice gene OsPOK1 is as shown in SEQ ID NO. 2. According to the invention, the new application of the rice gene OsPOK1 in regulation and control of the rice grain shape is found, a constructed rice modified plant containing the modified rice gene OsPOK1 disclosed by the invention can significantly increase the grain length and yield traits of rice grains, and the improvement of the rice grain shape and yield provides a new gene resource for rice breeding.

The application provides a cold-resistant gene TrWRKY10a540, an expression vector, application and breeding method thereof, and belongs to the field of plant molecular genetics and genetic improvement. Specifically disclosed is a cold-resistant gene TrWRKY10a540 and an expression vector derived from Trifolium repens L. and application thereof in improving the cold resistance of plants, and the problem of insufficient research on low-temperature stress related genes of Trifolium repens in the prior art is solved. The application further discloses a breeding method for regulating the cold stress resistance of plants by the cold-resistant gene TrWRKY10a540, and research results show that the gene can significantly improve the cold resistance and antioxidant level of plants under low-temperature stress conditions, and has good functional effects. The application can be widely applied to plant molecular breeding, is particularly suitable for improving the low-temperature resistance of economic crops such as forage grasses, provides a new genetic resource for the breeding of cold-resistant plants, and has important significance for revealing the regulation mechanism of the cold resistance of plants.

The application belongs to the technical field of plant molecular genetic engineering, and particularly relates to a FveDRD1 gene for regulating sugar accumulation in strawberry fruits and application thereof. The sequence of the FveDRD1 gene is shown as SEQ ID NO:1, and the amino acid sequence encoded by the FveDRD1 gene is shown as SEQ ID NO:2. The FveDRD1 gene can positively regulate sugar accumulation, such as sucrose, glucose and fructose, in strawberry fruits. By inhibiting or eliminating the expression of the FveDRD1 gene in the strawberry fruits, the sugar accumulation in the fruits can be reduced, and the sweetness can be reduced. The application provides a new genetic resource for improving the quality of strawberries.

The invention discloses a cucumber CsCCD1 gene and application of the cucumber CsCCD1 gene in regulating and controlling the color of cucumber pulp. According to the invention, a CCD family gene CsCCD1 is cloned from cucumber, and the cucumber CsCCD1 gene has the function of regulating and controlling the pulp color for the first time. A coding region of the CsCCD1 gene is edited by utilizing a CRISPR-Cas9 technology, and after the CsCCD1 is mutated, the color of the cucumber pulp is obviously yellowed, and the carotenoid content of the fruit is obviously improved. The invention not only lays an important foundation for revealing genetic and biological mechanisms of cucumber pulp color, but also provides a new gene resource for directional improvement of cucumber varieties and cultivation of high-quality new varieties, lays a theoretical foundation for later molecular breeding work, and has important application value.

The present invention relates to the field of gene engineering technology, and specifically to a gene vector mediated by CsCMV, and a preparation method and application thereof. Based on the pCsCMV-CM vector, the present invention successfully inserts the Bsm BI site into the non-coding region (UTR) after the stop codon (TAG) at the 3' end of CsCMV CP by the In-Fusion seamless cloning method, and constructs an infectious cloning pCsCMV-Bs vector based on the Golden gate Cloning strategy, which can quickly and high-throughput clone the target gene fragment into the viral genome, and is applied to cassava gene silencing and gene editing with higher stability and longer phenotypic duration, providing a new genetic tool for rapid and efficient gene function verification and directional improvement of cassava traits.

The invention discloses application of a lotus NnXTH2 gene to promotion of dwarfing and compactness of plant types and enhancement of rachis mechanics, and belongs to the technical field of biology. And the NnXTH2 gene is used for coding a protein as shown in SEQ ID NO. 4. The research finds that the rachis mechanics of the transgenic tobacco strain overexpressing the NnXTH2 gene is obviously enhanced, the plant height of the plant is reduced, the internode spacing is shortened, and the plant type of the plant is dwarfed and compact. The result shows that overexpression of the NnXTH2 gene can significantly enhance the rachis mechanics of the plant, shorten the plant height and internode spacing of the plant, make the plant type dwarf and compact, and better meet the plant type and rachis quality requirements of fresh cut flowers. According to the invention, the function of the NnXTH2 gene in regulation and control of plant type and rachis mechanics is defined for the first time, and new genetic resources and theoretical basis are provided for cultivating high-quality cut flower plants with rachis not easy to break and higher ornamental value.

The invention provides a VabHLH016 gene capable of improving cold resistance of plants and application of the VabHLH016 gene, and belongs to the technical field of molecular breeding. The invention provides a VabHLH016 gene derived from vitis amurensis and an application scheme of the VabHLH016 gene, so that the cold resistance of a receptor plant can be effectively improved. The method has the direct effects that the plant treated by the technology shows stronger survival activity and lighter cold injury symptom when encountering a low-temperature environment, and can better maintain a normal physiological state. The invention provides a new genetic resource and technical approach for solving the cultivation limitation of thermophilic crops such as grapes in high latitude or cold regions, and has important application value for breeding new cold-resistant varieties and ensuring stable yield and income increase of fruit tree industry.

The present invention discloses a rape RNA processing factor NCBP gene and its application, and relates to the field of gene engineering technology. The gene is located on the A10 chromosome of rape and has a nucleotide sequence as shown in SEQ ID NO.1, or has a nucleotide sequence complementary to the sequence shown in SEQ ID NO.1. The present invention discloses for the first time the use of the NCBP gene in regulating the flowering period of plants. The rape NCBP gene family is gene-edited by genetically transforming the Brassica napus variety Westar using CRISPR‑Cas9 technology, and further screening is performed to obtain homozygous mutant materials containing target gene mutations. The mutant materials show late flowering, and the overexpression of NCBP.A10 in Arabidopsis shows early flowering, which confirms the use of the NCBP gene in regulating the flowering period of rape. The present invention provides new gene resources and new ideas for cultivating Brassica napus with an appropriate growth period.