47 results about "New genetics" patented technology

The invention discloses a chloroplast RNA edited gene OsPPR7 for temperature-sensitive regulation and control of rice and application of the gene OsPPR7, and belongs to the technical field of gene engineering. The CDS sequence of the OsPPR7 gene is as shown in SEQ ID NO. 1. The OsPPR7 is an RNA (Ribonucleic Acid) editing factor which has a bidirectional regulation and control function and is sensitive to temperature. Editing of multiple sites on the rpoB gene is negatively regulated and controlled, and meanwhile RNA editing of multiple genes such as ndhB and ndhD which are crucial to the chloroplast function is positively regulated and controlled under high-temperature stress. Under the stress of high temperature of 35 DEG C, the loss of functions can lead to obvious reduction of RNA editing efficiency of a plurality of ndh genes, and serious yellowing of seedlings is caused. The key function of the rice OsPPR7 gene in regulation and control of rice growth and development and high-temperature response is clarified for the first time, the importance of the OsPPR7 gene in maintenance of chloroplast functions under high-temperature stress is clarified, and a new gene resource and a technical path are provided for cultivation of new varieties of high-temperature-resistant rice.

The invention belongs to the technical field of plant genetic engineering, and particularly discloses application of an important member of a potato mitogen-activated protein kinase (MAPK) family, namely an StMKK9 gene in regulation and control of maturity and cold resistance of potatoes. CDNA of a cultivar Desiree leaf is used as a template, the potato StMKK9 gene is separated and cloned, an over-expression vector is constructed, and the over-expression vector is used for expression of the potato. Genetic transformation of the potatoes is further carried out, it is found that the overexpressed StMKK9 gene can promote prematurity of the potatoes, meanwhile, the cold resistance of potato plants can be improved, and a new genetic material and a theoretical basis are provided for analysis of a potato tuberization molecular mechanism and improved breeding of the potatoes.

The invention discloses an application of an overexpressed CrOASTL1 gene in improving the soluble protein content of leguminous forage, and belongs to the technical field of biology. 1, and the CDS sequence of the CrOASTL1 gene is as shown in SEQ ID NO. 2. The method comprises the following steps: cloning a CrOASTL1 gene segment, constructing a plant overexpression vector, carrying out plant transgenosis operation, and introducing into leguminous forage plant cells to obtain a leguminous forage transgenosis line with the overexpressed CrOASTL1 gene. The CrOASTL1 gene is over-expressed in medicago sativa, and the soluble protein content of leaves of the medicago sativa is remarkably increased by about 19%. The CrOASTL1 gene of chlamydomonas reinhardtii is applied to quality improvement of leguminous forage for the first time, the technical obstacle that the genetic relationship of species is relatively far is overcome, a brand new gene resource and an efficient technical approach are provided for cultivating a new variety of leguminous forage with high protein and high nutritional value, and the method has important significance in promoting sustainable development of grass and animal husbandry.

The application discloses a corn kernel size related protein SSRP1 and application thereof. The technical problem solved is how to regulate the size of corn kernels. Specifically disclosed is the application of a protein, a substance for regulating the expression of a coding gene of the protein, or a substance for regulating the activity or content of the protein in regulating the size of plant kernels and / or preparing a product for regulating the size of plant kernels and / or plant breeding and / or preparing a plant breeding product, the protein being a protein as shown in sequence 3 in the sequence listing. The application provides a new genetic resource for the study of the size of plant kernels, in particular corn kernels, and will play an important role in the application in the field of corn seed production and breeding.

The invention relates to the field of plant genetic engineering, in particular to novel application of a soybean ACR gene family member GmACR39 in improving saline-alkaline tolerance of plants. The invention provides application of a separated gene in preparation of a product for improving saline-alkaline tolerance of plants, and the gene encodes a nucleotide sequence shown in SEQ ID NO: 1 or encodes a protein which has at least 80% identity with SEQ ID NO: 2 and has a function of improving saline-alkaline tolerance of plants. The expression of the gene is up-regulated under saline-alkaline stress, and the saline-alkaline tolerance of a transgenic plant can be remarkably improved by over-expression of the gene. The invention also provides an expression vector containing the gene, an sgRNA molecule targeting a promoter of the gene, a CRISPR activation system, and related reagents, kits, a method for cultivating saline-alkaline tolerant plants and detection means. The invention provides a new gene resource and a molecular breeding tool for cultivating new varieties of saline-alkaline tolerant crops.

The application provides a cold-resistant gene TrWRKY10a540, an expression vector, application and breeding method thereof, and belongs to the field of plant molecular genetics and genetic improvement. Specifically disclosed is a cold-resistant gene TrWRKY10a540 and an expression vector derived from Trifolium repens L. and application thereof in improving the cold resistance of plants, and the problem of insufficient research on low-temperature stress related genes of Trifolium repens in the prior art is solved. The application further discloses a breeding method for regulating the cold stress resistance of plants by the cold-resistant gene TrWRKY10a540, and research results show that the gene can significantly improve the cold resistance and antioxidant level of plants under low-temperature stress conditions, and has good functional effects. The application can be widely applied to plant molecular breeding, is particularly suitable for improving the low-temperature resistance of economic crops such as forage grasses, provides a new genetic resource for the breeding of cold-resistant plants, and has important significance for revealing the regulation mechanism of the cold resistance of plants.

The application belongs to the technical field of plant molecular genetic engineering, and particularly relates to a FveDRD1 gene for regulating sugar accumulation in strawberry fruits and application thereof. The sequence of the FveDRD1 gene is shown as SEQ ID NO:1, and the amino acid sequence encoded by the FveDRD1 gene is shown as SEQ ID NO:2. The FveDRD1 gene can positively regulate sugar accumulation, such as sucrose, glucose and fructose, in strawberry fruits. By inhibiting or eliminating the expression of the FveDRD1 gene in the strawberry fruits, the sugar accumulation in the fruits can be reduced, and the sweetness can be reduced. The application provides a new genetic resource for improving the quality of strawberries.

The invention discloses application of a lotus NnXTH2 gene to promotion of dwarfing and compactness of plant types and enhancement of rachis mechanics, and belongs to the technical field of biology. And the NnXTH2 gene is used for coding a protein as shown in SEQ ID NO. 4. The research finds that the rachis mechanics of the transgenic tobacco strain overexpressing the NnXTH2 gene is obviously enhanced, the plant height of the plant is reduced, the internode spacing is shortened, and the plant type of the plant is dwarfed and compact. The result shows that overexpression of the NnXTH2 gene can significantly enhance the rachis mechanics of the plant, shorten the plant height and internode spacing of the plant, make the plant type dwarf and compact, and better meet the plant type and rachis quality requirements of fresh cut flowers. According to the invention, the function of the NnXTH2 gene in regulation and control of plant type and rachis mechanics is defined for the first time, and new genetic resources and theoretical basis are provided for cultivating high-quality cut flower plants with rachis not easy to break and higher ornamental value.

The invention provides a VabHLH016 gene capable of improving cold resistance of plants and application of the VabHLH016 gene, and belongs to the technical field of molecular breeding. The invention provides a VabHLH016 gene derived from vitis amurensis and an application scheme of the VabHLH016 gene, so that the cold resistance of a receptor plant can be effectively improved. The method has the direct effects that the plant treated by the technology shows stronger survival activity and lighter cold injury symptom when encountering a low-temperature environment, and can better maintain a normal physiological state. The invention provides a new genetic resource and technical approach for solving the cultivation limitation of thermophilic crops such as grapes in high latitude or cold regions, and has important application value for breeding new cold-resistant varieties and ensuring stable yield and income increase of fruit tree industry.

The invention discloses a TRIM50 mutant gene of Jingmen green-shell laying hens and application of the TRIM50 mutant gene in regulation and control of cell growth, and belongs to the technical field of gene engineering. According to the invention, a TRIM50 mutant gene is identified in a group of Jingmen green-shell laying hens, and the nucleotide sequence of the TRIM50 mutant gene is shown as SEQ ID NO.4. Compared with a chicken TRIM50 wild type gene in NCBI (National Center of Biotechnology Information), the mutant gene has no obvious influence on cellular morphology and protein localization; however, the mutant gene can significantly promote cell proliferation, improve cell viability and inhibit cell apoptosis. According to the invention, a theoretical basis is provided for research on cell proliferation and apoptosis mechanisms of the Jingmen green-shell laying hens, a technical support is provided for construction of a high-activity cell model, and a new genetic resource is provided for identification of animal growth performance and molecular assisted breeding.

The invention relates to an astaxanthin-producing engineering bacterium as well as a construction method and application thereof. According to the astaxanthin-producing engineering bacterium, a crtE gene, a crtI gene, a crtB gene, a crtY gene, a crtZ gene, a crtW gene, a dxs gene and an idi gene are over-expressed. A brand-new genetic modification strategy is designed and comprises the steps that genes in an astaxanthin synthesis route are integrated to an escherichia coli genome, engineering bacteria for producing the astaxanthin are constructed, supply of astaxanthin synthesis precursors is improved by expressing dxs genes and idi genes on the genome, the yield of the astaxanthin is further increased, in addition, a specific crtW gene source is controlled, and the yield of the astaxanthin is increased. According to the present invention, the gene expression quantities of the crtY, the crtZ and the crtW, the astaxanthin yield can be further improved, the maximum shake flask level yield can achieve 27.4 mg / L, and the gene expression on the chromosome is further beneficial to the amplification to the industrial production.

The invention discloses application of an ASF1 gene to improvement of plant disease resistance or cultivation of disease-resistant plants, and belongs to the technical field of gene engineering. The ASF1 gene comprises an ASF1A gene and an ASF1B gene, the nucleotide sequence of the ASF1A gene is shown as SEQ ID NO. 1, and the nucleotide sequence of the ASF1B gene is shown as SEQ ID NO. 2. Phenotypes of ASF1A single knockout strains, ASF1B single knockout strains, ASF1A double knockout strains and ASF1B double knockout strains and influences on rice disease resistance are studied, and results show that compared with a wild type, a mutant plant is short and small, and the phenotype of the double knockout mutant is more obvious than that of a single knockout mutant; the single knockout strain and the double knockout strain both can improve the resistance of the rice to the xanthomonas oryzae sclerotiorum, and the double knockout strain has stronger resistance, so that a new genetic resource is provided for research on disease-resistant breeding of the rice.

The invention relates to application of a TaGRF2-A gene in enhancing heat resistance of wheat, and belongs to the field of plant genetic engineering. Specifically, the wheat TaGRF2-A gene participating in positive regulation and control of high-temperature stress resistance is cloned in wheat, so that the wheat TaGRF2-A gene is overexpressed, and compared with a wild plant, a wheat plant with the overexpressed TaGRF2-A gene shows higher high-temperature resistance; the TaGRF2-A gene enhances the tolerance of wheat under a high-temperature condition in manners of regulating the stomatal conductance of plants, optimizing the photosynthesis efficiency, improving the water utilization efficiency and the like, and a new genetic engineering modification means is provided for cultivating a new wheat variety with high yield, stable yield and high temperature resistance.

This invention belongs to the field of plant genetic engineering and relates to the application and method of reducing the expression of the Zm7649 gene in improving plant stress resistance. This invention utilizes an EMS mutant with premature termination of expression. Zm7649-ems Heterologous overexpression with Arabidopsis thaliana Zm7649-OE Verified Zm7649 The function of negatively regulating salt stress and high temperature stress was studied, and its preliminary analysis was performed. Zm00001d027649 The molecular mechanism regulating stress involves the interaction between Zm7649 and a maize catalase (Zm4848), with ubiquitination degrading Zm4848 and reducing its protein stability. This invention provides new genetic resources and theoretical basis for maize stress-resistance breeding or germplasm innovation.

This invention discloses overexpression cysK The application of genes in increasing the soluble protein content of leguminous forage grasses belongs to the field of biotechnology. cysK The CDS sequence of the gene is shown in SEQ ID NO. 2. A plant overexpression vector containing the nucleotide sequence shown in SEQ ID NO. 2 was constructed, and the plant overexpression vector was introduced into leguminous forage plant cells to obtain overexpressed genes. cysK Transgenic leguminous forage lines with overexpressed genes in alfalfa. cysK The gene significantly increases the soluble protein content in its leaves by approximately 28%. This invention is the first to utilize Escherichia coli derived from prokaryotes. cysK The successful application of genes to the quality improvement of leguminous forage grasses has overcome the technical barrier of distant species kinship, providing new genetic resources and efficient technical approaches for breeding new varieties of leguminous forage grasses with high protein and high nutritional value, which is of great significance for promoting the sustainable development of the grass animal husbandry industry.

Provided are cells that are resistant to mobile genetic elements or horizontal gene transfer, and methods for obtaining said cells. Also provided are methods for preventing the horizontal transfer of genetic information between a mobile genetic element and a first cell, cells making use of new genetic codons schemes and related subject matter, kits comprising mutually orthogonal cells, and mobile genetic elements. Also provided are methods of altering the susceptibility of a gene to mutations that alter the encoded amino acid sequence, methods for evolving or improving a protein, and methods for rendering a target gene more resistant to mutation. Additionally provided are uses of the cells for making polymers and methods comprising using the cells for making polymers.

This invention discloses a ZmLG2 gene mutant and its application in regulating maize root architecture and nitrogen utilization efficiency, relating to the field of biotechnology. The ZmLG2 gene mutant has the DNA sequence shown in SEQ ID No. 3. Compared with the wild-type ZmLG2 gene SEQ ID No. 1, it contains the following mutations: a 21-base deletion at the end of the first exon CDS1; a complete deletion of the first intron intron1; a 189-base deletion at the beginning of the second exon CDS2; a T→G mutation at +2284, a T→C mutation at +2337, a G→A mutation at +2344, a C→A mutation at +2361, and a C→A mutation at +2394 downstream of the DNA start site; and a 1-base deletion at +2838. The ZmLG2 mutant plays a key role in regulating maize root architecture and improving nitrate absorption and utilization efficiency. It has significant biological functions and application value, providing new genetic resources and technical support for breeding crop varieties with high nitrogen fertilizer utilization efficiency. It also has broad application prospects in promoting green and sustainable agricultural development.

The invention discloses an application of an AP2 transcription factor gene Chalk8 in regulation and control of chalkiness traits of rice, a nucleotide sequence of a Chalk8 gene coding region is shown as SEQ ID NO.1, an amino acid sequence is shown as SEQ ID NO.2, and the chalkiness traits comprise chalkiness grain rate and chalkiness degree. According to the invention, the AP2 transcription factor Chalk8 is found to be a key gene for influencing chalkiness difference between different rice varieties through a whole genome association analysis method for the first time. A gene editing technology is further utilized to create a Chalk8 gene knockout mutant, a Chalk8 gene overexpression strain is obtained through a transgenic technology, and a novel method for reducing chalkiness of rice by enhancing expression of the Chalk8 gene is verified. Besides, a Chalk8 allele related to chalkiness variation between rice varieties is identified through haplotype analysis, the Chalk8 allele comprises an excellent haplotype capable of remarkably reducing chalkiness, and a new gene resource is provided for rice quality improvement.

This invention discloses a mutant TRIM50 gene in Jingmen green-shelled laying hens and its application in regulating cell growth, belonging to the field of genetic engineering technology. This invention identified a mutant TRIM50 gene in a Jingmen green-shelled laying hen population, its nucleotide sequence of which is shown in SEQ ID NO.4. Compared to the wild-type chicken TRIM50 gene in NCBI, this mutant gene has no significant effect on cell morphology and protein localization; however, it significantly promotes cell proliferation, increases cell viability, and inhibits apoptosis. This invention provides a theoretical basis for studying the mechanisms of cell proliferation and apoptosis in Jingmen green-shelled laying hens, provides technical support for constructing high-viability cell models, and provides new genetic resources for identifying animal growth performance and molecular-assisted breeding.

This invention discloses oats AsNF-YA14 The application of genes and their encoded proteins belongs to the field of bioengineering technology. This invention discloses for the first time the properties of oats... AsNF-YA14 A novel function of genes in regulating plant flowering time. Experiments show that... AsNF-YA14 The gene is highly expressed in oat spikelets, and its expression level gradually increases with the growth process, suggesting that it may be involved in flowering regulation. Overexpression in Arabidopsis thaliana... AsNF-YA14 The transgenic plants exhibited a significant early-flowering phenotype, blooming approximately 11 days earlier than the wild-type plants, while also showing a significant reduction in the number of rosette leaves. These results indicate that... AsNF-YA14 Genes have the function of promoting earlier flowering in plants. This invention provides new genetic resources for the genetic improvement of plant flowering time, which can be used to breed new varieties of oats or other crops with early flowering and short growth periods. This is of great significance for expanding crop planting areas, increasing the multiple cropping index, and achieving stable and high yields.

The application belongs to the technical field of genetic engineering and plant disease control, and particularly relates to a gene for positively regulating plant disease resistance and application thereof. The gene is named SIT7, the nucleotide sequence of which is shown as SEQ ID NO. 1, and the amino acid sequence encoded by the gene is shown as SEQ ID NO. 2. The application proves that overexpression of the gene in plants can significantly enhance the disease resistance of the plants, which is manifested by the increase of SA accumulation in the plants and the increase of the expression level of defense-related genes, indicating that SIT7 is involved in the SA-regulated plant immune process. The application provides a brand-new genetic resource for genetic improvement of plant disease resistance, and has a good application prospect.

This invention discloses a gene that regulates the content of gliadin in rice grains. qPro7 This invention relates to the fields of genetic engineering and plant molecular breeding, and its applications. The amino acid sequence of the gene is shown in SEQ ID NO.1. Through genome-wide association analysis, this invention identified and cloned the gene on rice chromosome 7. qPro7 By constructing genes qPro7 Knockout vectors and self-promoted overexpression vectors were used to transform rice, and the results showed that knockout vectors... qPro7 The gene can significantly reduce the content of prolactin in rice grains, while overexpression qPro7 The gene significantly increases the content of prolamins in rice grains; this invention provides new genetic resources for the genetic improvement of rice quality, especially the regulation of protein content, and has important application value.

The invention discloses application of a fluctuating light response gene in regulation and control of rice growth, and belongs to the technical field of gene engineering. The nucleotide sequence of the fluctuation light response gene is as shown in SEQ ID NO. 1. The gene for regulating and controlling the fluctuation light response is excavated, and the gene can regulate and control the photosynthesis of the rice, so that the heading time, plant height and other characters of the rice are regulated and controlled. After the fluctuating photoresponse gene is knocked out, the heading time of the rice is late, but the maturing rate and the yield are kept unchanged, the filling efficiency is improved, and stable yield formation is facilitated; the plant height of the rice is reduced, so that lodging resistance is facilitated; and the number of abortive rice grains can be reduced. The invention provides a new genetic resource and theoretical basis for improving the photosynthetic efficiency of crops and lodging-resistant breeding of crops under natural conditions.

The application discloses a growth and development gene of Gossypium hirsutum and application thereof. The gene Gohir.A08G240900 related to growth and development is mined out by reverse transcription of RNA of Gossypium hirsutum, a nucleotide sequence of the gene is shown as SEQ ID No. 1, the gene can affect plant height of Gossypium hirsutum, and the expression amount of the gene is more in Gossypium hirsutum with high plant height, and the expression amount of the gene is significantly reduced in Gossypium hirsutum with short plant height. Meanwhile, through a virus-induced gene silencing (VIGS) experiment, it is found that the gene Gohir.A08G240900 causes growth and development of a plant to be damaged after silencing, thereby affecting growth of the plant, so that a phenotype of plant height being significantly short is shown, and it is shown that the gene Gohir.A08G240900 is a potential target for regulating plant height, and a new genetic basis for improving plant height of Gossypium hirsutum is provided.

This invention relates to the field of plant genetic engineering, specifically to the MMR1 protein, its encoding gene, and its applications in regulating ROS homeostasis in the root tip meristem of plants. This invention involves knocking out the MMR1 gene to obtain stable transgenic lines of Arabidopsis thaliana. mmr1-1, mmr1-2 and build MMR1 Gene replacement lines and MMRI Gene overexpression lines; mutants compared to wild type mmr1-1, mmr1-2 The results showed shortened root length, shortened root tip meristem, and reduced root tip division ability; reintroduced lines indicated that the MMR1 protein was located in the mitochondria; the roots of the overexpressing lines were longer than those of the wild type, indicating... MMR1 The gene plays an important role in root elongation, root apical meristem development, and root apical meristem cell activity in Arabidopsis thaliana. This invention fills a gap in the field of mitochondrial-localized protein regulation of root development and ROS homeostasis, providing new genetic resources for root improvement and transgenic breeding.

The invention discloses a ZmRL10 gene and application thereof in regulation and control of corn plant height and shading response, and relates to the technical field of plant genetic engineering, the nucleotide sequence of the ZmRL10 gene is as shown in SEQ ID NO.1, and the amino acid sequence of encoded protein of the ZmRL10 gene is as shown in SEQ ID NO.2. According to the invention, herbicide resistance screening of a transformation receptor is endowed through a Bar marker gene, and the expression level of the ZmRL10 gene is detected through qPCR analysis, so that positive seedlings can be accurately and rapidly detected, and phenotypic observation is carried out on a ZmRL10 gene overexpressed corn plant, and it is found that compared with wild type corn LH244, the ZmRL10 gene overexpressed corn plant has the advantages that the yield of the ZmRL10 gene overexpressed corn plant is greatly improved; the overexpression corn plant containing the ZmRL10 gene shows the characteristic that the plant height is remarkably reduced, meanwhile, the sensitivity to shading light signals under the shading condition is remarkably weakened, the shading reaction is weakened, and the overexpression corn plant has a better plant type structure and higher adaptability under the close planting condition and has a good application prospect. A new genetic resource and a technical approach are provided for cultivating high-yield and close-planting-resistant corn varieties, and the crop yield is further increased.

The application discloses a genetic element for accelerating morphological differentiation of actinomycetes, and belongs to the fields of synthetic biology and molecular biology. eshA Gene overexpression vector pIB139-PermE-his- eshA -his is transformed into target actinomycetes streptomyces coelicolor, and an engineering strain is obtained through screening, and through plate phenotype observation and growth curve determination, the significant effect of gene overexpression is verified. eshA The overexpression strain O eshA S. coelicolor WT appears aerial hyphae and mature spores earlier. The application provides a simple and specific technical scheme for controlling the speed of morphological differentiation of actinomycetes, provides a new genetic element for accelerating morphological differentiation of actinomycetes, and has important application value.​

The invention discloses a corn meiosis recombinant antagonistic gene ZmRecQ4 and an application thereof. The ZmRecQ4 mutation can significantly improve the recombination level of corn, provides a new genetic tool for improving the recombination potential of different corn varieties, and also provides a feasible path for reducing the screening group scale, shortening the breeding period and accelerating the cultivation of good varieties in precise breeding.

The invention discloses a method and a system for integrating a chip and filling data to optimize genome selection, and belongs to the technical field of genome breeding. The method comprises the following steps: firstly, acquiring chip data and whole genome filling data; then executing a chip anchoring and filling expansion strategy: screening a core SNP set from chip data based on whole genome association analysis, and screening an additional significant SNP set from filling data; and finally, combining the two sets to form an optimized SNP set and carrying out genome selection analysis. The internal bottleneck that high-quality filling data is directly used for genome selection to cause reduction of accuracy is disclosed for the first time, through an innovative integration strategy, the prediction robustness is ensured by utilizing chip data, new genetic signals are explored by utilizing the filling data, advantage complementation is realized, the breeding value prediction accuracy is remarkably improved, and the breeding value prediction method is suitable for large-scale popularization and application. An effective scheme is provided for solving the application problem of high-density genotype data in breeding.

This invention relates to the field of plant genetic engineering technology, specifically to the application of HSFA1 protein or its encoding gene in regulating plant mesocotyl growth. This invention provides the application of the plant HSF family transcription factor HSFA1 in regulating the temperature-responsive growth of plant mesocotyls. HSFA1 is a negative regulator of the high-temperature response of mesocotyls. Under high environmental temperatures, the mesocotyl length of HSFA1 knockout mutants is significantly longer than that of the wild type, exhibiting greater sensitivity to high environmental temperatures. This has important application value in direct-seeded rice production under high-temperature conditions, providing new genetic resources and methods for breeding rice varieties suitable for direct seeding.