93 results about "New genetics" patented technology

The invention relates to the technical field of gene engineering, and discloses an METS2 gene for regulating and controlling the grain shape of rice, a protein and application of the METS2 gene, and the nucleotide sequence of the METS2 gene is shown as SEQ ID NO.1. The invention further discloses application of the METS2 gene in regulation and control of the rice grain shape. According to the METS2 gene for regulating and controlling the rice grain shape, the protein and the application of the METS2 gene, the METS2 gene is overexpressed in a rice plant, short-grain-shaped rice can be obtained, the short-grain-shaped rice is not prone to breakage in the processing process, and the quality of a finished product is high; a basis is provided for further clarification of the molecular mechanism of the rice grain shape, and new gene resources and breeding resources are provided for rice breeding.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a wheat transcription factor TaWRKY22 and a coding gene thereof in regulation and control of plant stripe rust resistance. The function of the wheat transcription factor TaWRKY22 is researched through molecular biology and genetics methods, it is found that knockout of the TaWRKY22 gene significantly improves the resistance of wheat to stripe rust, overexpression of the TaWRKY22 gene reduces the resistance of wheat to stripe rust, and then it is indicated that the wheat transcription factor TaWRKY22 plays a negative regulation role in wheat stripe rust resistance. Therefore, the TaWRKY22 gene is subjected to targeted modification through a gene editing technology, a new genetic material and a molecular breeding strategy can be provided for wheat stripe rust resistance breeding, and an important material and technical support are provided for breeding of wheat disease-resistant varieties.

The invention discloses a method for obtaining leymus chinensis mutants by using heavy ion beams to irradiate seed calluses, relates to the technical field of leymus chinensis breeding biology, and solves the three-low problems of low heading rate, low maturing rate and low germination rate of leymus chinensis seeds. After the leymus chinensis callus is subjected to induction culture, selecting faint yellow granular callus obtained by induction culture, and transferring the faint yellow granular callus into a culture dish filled with an induction culture medium for subculture; carrying out irradiation treatment by using a 5Gy carbon ion beam, transferring the callus subjected to irradiation treatment into a sterile culture bottle filled with a differential culture medium, and culturing to obtain adventitious buds; cutting off callus adhered to the base under a sterile condition, and inserting into a rooting culture medium for rooting culture after a rooting point is exposed; and after obvious root systems grow out, carrying out potting culture to obtain the leymus chinensis mutant. According to the invention, new genetic variation can be created, early in-vitro selection is realized, more effective mutants are obtained as new germplasm resources of leymus chinensis, and the breeding process of leymus chinensis is accelerated.

The invention belongs to the technical field of biology, and particularly relates to a TaGAT2-1B gene resistant to wheat stripe rust and application of the TaGAT2-1B gene, and the nucleotide sequence of the TaGAT2-1B gene is shown as SEQ ID NO.3. It is found that the TaGAT2-1B gene and the encoding protein thereof can effectively improve the wheat stripe rust resistance, the TaGAT2-1B gene is a new stripe rust resistance gene in the wheat adult-plant stage, infection of various stripe rust strains can be effectively resisted, the field phenotype of the TaGAT2-1B gene shows the characteristic of slow rust, and the TaGAT2-1B gene and the encoding protein thereof have good application prospects. The discovery of the gene provides a new genetic resource for the research of the wheat stripe rust resistance mechanism and the wheat stripe rust resistance breeding.

The invention relates to the technical field of plant molecular genetic engineering, in particular to an FvemiR172c gene for regulating and controlling anthocyanin accumulation of strawberry fruits and application of the FvemiR172c gene. The nucleotide sequence of the FvemiR172c gene is as shown in SEQ ID NO. 1 (sequence identifier number 1). The invention discloses a nucleotide sequence of an FvemiR172c gene for regulating and controlling the anthocyanin content of strawberry fruits, and experiments show that the FvemiR172c gene or an expression vector containing the FvemiR172c gene can be applied to regulation and control of the anthocyanin content of the strawberry fruits so as to regulate and control the color of the strawberry fruits. The invention provides a new gene resource for creating different colors of strawberries and molecular breeding for promoting / inhibiting anthocyanin accumulation.

The invention provides application of a poplar PtoHsfB1 gene in plant growth, and belongs to the technical field of gene engineering. The function of promoting plant growth of the populus tomentosa gene PtoHsfB1 is found for the first time. Experimental results show that the expression quantity of the PtoHsfB1 gene in the transgenic populus tomentosa is greatly improved compared with that of non-transgenic populus tomentosa, the height, dry weight and fresh weight of a transgenic plant are higher than those of a non-transgenic plant, ABA synthesis and signal transduction can be reduced through overexpression of the gene PtoHsfB1, growth and development of the populus tomentosa are promoted, and the yield of the populus tomentosa is increased. Therefore, a new genetic resource is provided for improvement and molecular breeding of the poplar and other woody plants.

The invention provides an application of a citrus transcription factor CsAGL9 gene and a protein coded by the citrus transcription factor CsAGL9 gene in regulating and controlling the content of citric acid in citrus fruits, the nucleotide sequence of the CsAGL9 gene is shown as SEQ ID NO.1, and the amino acid sequence of the protein is shown as SEQ ID NO.2. The content of citric acid in the fruits can be reduced through overexpression in the oranges; the content of citric acid in fruits can be increased by silencing the gene. It is further verified that the gene promotes the activity of the citrus citric acid transporter CsTCT1. The discovery provides a new gene resource for citrus quality improvement and molecular design breeding, and provides an efficient tool for fruit quality improvement.

The invention belongs to the technical field of biology, and particularly relates to application of a GmNIC1 gene and a GmNIC2 gene to regulation and control of soybean cyst nematode resistance of plants, and the nucleotide sequence of the GmNIC1 gene is shown as SEQ ID NO.1; the nucleotide sequence of the GmNIC2 gene is as shown in SEQ ID NO. 2. The invention discloses the negative regulation effect of GmNIC1 and GmNIC2 genes in the resistance of soybean to cyst nematode (SCN) for the first time, and by constructing gene silencing mutants of GmNIC1 and GmNIC2, it is verified that the resistance of soybean to SCN can be remarkably enhanced due to function deficiency of the gene family. The application provides a new target for deep understanding of a soybean-nematode interaction mechanism, and provides a brand new gene resource and technical path for molecular design breeding of resistant soybeans.

The invention discloses a chloroplast RNA edited gene OsPPR7 for temperature-sensitive regulation and control of rice and application of the gene OsPPR7, and belongs to the technical field of gene engineering. The CDS sequence of the OsPPR7 gene is as shown in SEQ ID NO. 1. The OsPPR7 is an RNA (Ribonucleic Acid) editing factor which has a bidirectional regulation and control function and is sensitive to temperature. Editing of multiple sites on the rpoB gene is negatively regulated and controlled, and meanwhile RNA editing of multiple genes such as ndhB and ndhD which are crucial to the chloroplast function is positively regulated and controlled under high-temperature stress. Under the stress of high temperature of 35 DEG C, the loss of functions can lead to obvious reduction of RNA editing efficiency of a plurality of ndh genes, and serious yellowing of seedlings is caused. The key function of the rice OsPPR7 gene in regulation and control of rice growth and development and high-temperature response is clarified for the first time, the importance of the OsPPR7 gene in maintenance of chloroplast functions under high-temperature stress is clarified, and a new gene resource and a technical path are provided for cultivation of new varieties of high-temperature-resistant rice.

The invention belongs to the technical field of plant molecular genetic engineering, and particularly relates to a FaAL6 gene related to strawberry fruit hardness and application of the FaAL6 gene. The nucleotide sequence of the FaAL6 gene is as shown in SEQ ID NO. 1. The invention discloses a nucleotide sequence of a FaAL6 gene for regulating and controlling the hardness of strawberry fruits and an amino acid sequence coded by the FaAL6 gene, and experiments show that the FaAL6 gene or a construct or a host cell containing the FaAL6 gene can be applied to regulating and controlling the hardness of the strawberry fruits. The invention provides a new gene resource for molecular breeding for delaying strawberry fruit softening and improving strawberry fruit storage and transportation performance.

The invention belongs to the technical field of plant genetic engineering, and particularly discloses application of an important member of a potato mitogen-activated protein kinase (MAPK) family, namely an StMKK9 gene in regulation and control of maturity and cold resistance of potatoes. CDNA of a cultivar Desiree leaf is used as a template, the potato StMKK9 gene is separated and cloned, an over-expression vector is constructed, and the over-expression vector is used for expression of the potato. Genetic transformation of the potatoes is further carried out, it is found that the overexpressed StMKK9 gene can promote prematurity of the potatoes, meanwhile, the cold resistance of potato plants can be improved, and a new genetic material and a theoretical basis are provided for analysis of a potato tuberization molecular mechanism and improved breeding of the potatoes.

The invention discloses an application of an overexpressed CrOASTL1 gene in improving the soluble protein content of leguminous forage, and belongs to the technical field of biology. 1, and the CDS sequence of the CrOASTL1 gene is as shown in SEQ ID NO. 2. The method comprises the following steps: cloning a CrOASTL1 gene segment, constructing a plant overexpression vector, carrying out plant transgenosis operation, and introducing into leguminous forage plant cells to obtain a leguminous forage transgenosis line with the overexpressed CrOASTL1 gene. The CrOASTL1 gene is over-expressed in medicago sativa, and the soluble protein content of leaves of the medicago sativa is remarkably increased by about 19%. The CrOASTL1 gene of chlamydomonas reinhardtii is applied to quality improvement of leguminous forage for the first time, the technical obstacle that the genetic relationship of species is relatively far is overcome, a brand new gene resource and an efficient technical approach are provided for cultivating a new variety of leguminous forage with high protein and high nutritional value, and the method has important significance in promoting sustainable development of grass and animal husbandry.

The invention discloses a gene OsNST2 for transporting nucleotide sugar and application of the gene OsNST2. A nucleotide sequence of a coding region of the gene is as shown in SEQ ID No. 1, and an amino acid sequence coded by the gene is as shown in SEQ ID No. 2. It is found for the first time that the growth, development and yield of rice are affected by the OsNST2 by regulating and controlling formation of rice cell walls, an OsNST2 gene knock-down strain is created through an RNAi technology, an OsNST2 gene overexpression strain is obtained through an overexpression transgenic technology, it is verified that the OsNST2 gene is a new gene for regulating and controlling formation of the rice cell walls, and a new gene resource is provided for high yield of rice.

The invention relates to the technical field of plant genetic engineering, in particular to application of a rice OsERF141 protein and a coding gene thereof in improving low-temperature tolerance of plants. Experiments show that the low-temperature resistance of an OsERF141 function deletion mutant strain is remarkably reduced, and the low-temperature resistance of an overexpressed OsERF141 transgenic strain is remarkably improved. The invention provides a new gene resource for the cold resistance problem of rice, and has important significance for the molecular mechanism research of low-temperature stress and the cultivation of new low-temperature-resistant varieties.

The application discloses a corn kernel size related protein SSRP1 and application thereof. The technical problem solved is how to regulate the size of corn kernels. Specifically disclosed is the application of a protein, a substance for regulating the expression of a coding gene of the protein, or a substance for regulating the activity or content of the protein in regulating the size of plant kernels and / or preparing a product for regulating the size of plant kernels and / or plant breeding and / or preparing a plant breeding product, the protein being a protein as shown in sequence 3 in the sequence listing. The application provides a new genetic resource for the study of the size of plant kernels, in particular corn kernels, and will play an important role in the application in the field of corn seed production and breeding.

The invention relates to the field of plant genetic engineering, in particular to novel application of a soybean ACR gene family member GmACR39 in improving saline-alkaline tolerance of plants. The invention provides application of a separated gene in preparation of a product for improving saline-alkaline tolerance of plants, and the gene encodes a nucleotide sequence shown in SEQ ID NO: 1 or encodes a protein which has at least 80% identity with SEQ ID NO: 2 and has a function of improving saline-alkaline tolerance of plants. The expression of the gene is up-regulated under saline-alkaline stress, and the saline-alkaline tolerance of a transgenic plant can be remarkably improved by over-expression of the gene. The invention also provides an expression vector containing the gene, an sgRNA molecule targeting a promoter of the gene, a CRISPR activation system, and related reagents, kits, a method for cultivating saline-alkaline tolerant plants and detection means. The invention provides a new gene resource and a molecular breeding tool for cultivating new varieties of saline-alkaline tolerant crops.

The application provides a cold-resistant gene TrWRKY10a540, an expression vector, application and breeding method thereof, and belongs to the field of plant molecular genetics and genetic improvement. Specifically disclosed is a cold-resistant gene TrWRKY10a540 and an expression vector derived from Trifolium repens L. and application thereof in improving the cold resistance of plants, and the problem of insufficient research on low-temperature stress related genes of Trifolium repens in the prior art is solved. The application further discloses a breeding method for regulating the cold stress resistance of plants by the cold-resistant gene TrWRKY10a540, and research results show that the gene can significantly improve the cold resistance and antioxidant level of plants under low-temperature stress conditions, and has good functional effects. The application can be widely applied to plant molecular breeding, is particularly suitable for improving the low-temperature resistance of economic crops such as forage grasses, provides a new genetic resource for the breeding of cold-resistant plants, and has important significance for revealing the regulation mechanism of the cold resistance of plants.

The application belongs to the technical field of plant molecular genetic engineering, and particularly relates to a FveDRD1 gene for regulating sugar accumulation in strawberry fruits and application thereof. The sequence of the FveDRD1 gene is shown as SEQ ID NO:1, and the amino acid sequence encoded by the FveDRD1 gene is shown as SEQ ID NO:2. The FveDRD1 gene can positively regulate sugar accumulation, such as sucrose, glucose and fructose, in strawberry fruits. By inhibiting or eliminating the expression of the FveDRD1 gene in the strawberry fruits, the sugar accumulation in the fruits can be reduced, and the sweetness can be reduced. The application provides a new genetic resource for improving the quality of strawberries.

The invention discloses a cucumber CsCCD1 gene and application of the cucumber CsCCD1 gene in regulating and controlling the color of cucumber pulp. According to the invention, a CCD family gene CsCCD1 is cloned from cucumber, and the cucumber CsCCD1 gene has the function of regulating and controlling the pulp color for the first time. A coding region of the CsCCD1 gene is edited by utilizing a CRISPR-Cas9 technology, and after the CsCCD1 is mutated, the color of the cucumber pulp is obviously yellowed, and the carotenoid content of the fruit is obviously improved. The invention not only lays an important foundation for revealing genetic and biological mechanisms of cucumber pulp color, but also provides a new gene resource for directional improvement of cucumber varieties and cultivation of high-quality new varieties, lays a theoretical foundation for later molecular breeding work, and has important application value.

The present invention relates to the field of gene engineering technology, and specifically to a gene vector mediated by CsCMV, and a preparation method and application thereof. Based on the pCsCMV-CM vector, the present invention successfully inserts the Bsm BI site into the non-coding region (UTR) after the stop codon (TAG) at the 3' end of CsCMV CP by the In-Fusion seamless cloning method, and constructs an infectious cloning pCsCMV-Bs vector based on the Golden gate Cloning strategy, which can quickly and high-throughput clone the target gene fragment into the viral genome, and is applied to cassava gene silencing and gene editing with higher stability and longer phenotypic duration, providing a new genetic tool for rapid and efficient gene function verification and directional improvement of cassava traits.

The invention discloses application of a lotus NnXTH2 gene to promotion of dwarfing and compactness of plant types and enhancement of rachis mechanics, and belongs to the technical field of biology. And the NnXTH2 gene is used for coding a protein as shown in SEQ ID NO. 4. The research finds that the rachis mechanics of the transgenic tobacco strain overexpressing the NnXTH2 gene is obviously enhanced, the plant height of the plant is reduced, the internode spacing is shortened, and the plant type of the plant is dwarfed and compact. The result shows that overexpression of the NnXTH2 gene can significantly enhance the rachis mechanics of the plant, shorten the plant height and internode spacing of the plant, make the plant type dwarf and compact, and better meet the plant type and rachis quality requirements of fresh cut flowers. According to the invention, the function of the NnXTH2 gene in regulation and control of plant type and rachis mechanics is defined for the first time, and new genetic resources and theoretical basis are provided for cultivating high-quality cut flower plants with rachis not easy to break and higher ornamental value.

The invention provides a VabHLH016 gene capable of improving cold resistance of plants and application of the VabHLH016 gene, and belongs to the technical field of molecular breeding. The invention provides a VabHLH016 gene derived from vitis amurensis and an application scheme of the VabHLH016 gene, so that the cold resistance of a receptor plant can be effectively improved. The method has the direct effects that the plant treated by the technology shows stronger survival activity and lighter cold injury symptom when encountering a low-temperature environment, and can better maintain a normal physiological state. The invention provides a new genetic resource and technical approach for solving the cultivation limitation of thermophilic crops such as grapes in high latitude or cold regions, and has important application value for breeding new cold-resistant varieties and ensuring stable yield and income increase of fruit tree industry.

The invention discloses application of a rice nitrogen utilization gene NU3 in regulation and control of rice growth, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the rice nitrogen utilization gene NU3 disclosed by the invention is as shown in SEQ ID NO. 1; the regulation of rice growth refers to regulation of rice nitrogen utilization rate, regulation of rice seed quality and regulation of seed protein content. According to the invention, the expression mode of the gene NU3 in rice is identified, and a function deletion mutant of the gene NU3 is obtained by using a gene editing technology. The rice seedling growth amount of the functional deletion mutant is reduced, the thousand seed weight is obviously reduced, the protein content of seeds is reduced, and the nitrogen utilization rate is reduced. The invention provides new gene resources and research basis for rice nitrogen metabolism research, growth regulation mechanism research and seed quality regulation.

The invention discloses a TRIM50 mutant gene of Jingmen green-shell laying hens and application of the TRIM50 mutant gene in regulation and control of cell growth, and belongs to the technical field of gene engineering. According to the invention, a TRIM50 mutant gene is identified in a group of Jingmen green-shell laying hens, and the nucleotide sequence of the TRIM50 mutant gene is shown as SEQ ID NO.4. Compared with a chicken TRIM50 wild type gene in NCBI (National Center of Biotechnology Information), the mutant gene has no obvious influence on cellular morphology and protein localization; however, the mutant gene can significantly promote cell proliferation, improve cell viability and inhibit cell apoptosis. According to the invention, a theoretical basis is provided for research on cell proliferation and apoptosis mechanisms of the Jingmen green-shell laying hens, a technical support is provided for construction of a high-activity cell model, and a new genetic resource is provided for identification of animal growth performance and molecular assisted breeding.

The present invention provides a gene related to the yellow-green leaf phenotype of Wucai, the gene is a BcAFR gene, and the nucleotide sequence is shown in SEQ ID NO.1. The present invention also provides the use of the above gene in cultivating a new variety of Wucai with a yellow-green phenotype. At the same time, the present invention also provides a BcAFR gene-silenced plant and a method for regulating the yellow-green phenotype of Wucai leaves. The present invention screened the BcAFR gene in Wucai, and verified the role of the BcAFR gene in controlling the yellow-green phenotype of Wucai leaves. At the same time, the Wucai BcAFR gene-silenced plant was obtained through the VIGS experiment, and further verified that BcAFR can regulate the yellow-green phenotype of Wucai leaves, laying a foundation for exploring the molecular mechanism of Wucai leaf color, and providing new gene resources for the creation of breeding materials, and also providing important scientific basis and germplasm materials for the breeding of Wucai varieties with different leaf colors.

The invention relates to an astaxanthin-producing engineering bacterium as well as a construction method and application thereof. According to the astaxanthin-producing engineering bacterium, a crtE gene, a crtI gene, a crtB gene, a crtY gene, a crtZ gene, a crtW gene, a dxs gene and an idi gene are over-expressed. A brand-new genetic modification strategy is designed and comprises the steps that genes in an astaxanthin synthesis route are integrated to an escherichia coli genome, engineering bacteria for producing the astaxanthin are constructed, supply of astaxanthin synthesis precursors is improved by expressing dxs genes and idi genes on the genome, the yield of the astaxanthin is further increased, in addition, a specific crtW gene source is controlled, and the yield of the astaxanthin is increased. According to the present invention, the gene expression quantities of the crtY, the crtZ and the crtW, the astaxanthin yield can be further improved, the maximum shake flask level yield can achieve 27.4 mg / L, and the gene expression on the chromosome is further beneficial to the amplification to the industrial production.

The invention discloses an SNP molecular marker linked with the sucrose content of peanut seed kernels, a KASP primer group and application of the SNP molecular marker and the KASP primer group, and belongs to the technical field of molecular genetic breeding. The molecular marker SucB06 related to the sucrose content of the peanut seed kernels is screened, a KASP primer group is developed according to the molecular marker and the SucA06 molecular marker, a peanut variety with the high sucrose content of the seed kernels can be accurately distinguished from a peanut variety with the low sucrose content of the seed kernels by utilizing a KASP genetic typing technology, and the peanut variety with the low sucrose content of the seed kernels can be accurately distinguished. The method can be used for early identification and screening of peanut materials, the peanut breeding period can be shortened, the genetic resource improvement process is accelerated, and new genetic resources and theoretical basis are provided for molecular assisted breeding.

The invention discloses application of an ASF1 gene to improvement of plant disease resistance or cultivation of disease-resistant plants, and belongs to the technical field of gene engineering. The ASF1 gene comprises an ASF1A gene and an ASF1B gene, the nucleotide sequence of the ASF1A gene is shown as SEQ ID NO. 1, and the nucleotide sequence of the ASF1B gene is shown as SEQ ID NO. 2. Phenotypes of ASF1A single knockout strains, ASF1B single knockout strains, ASF1A double knockout strains and ASF1B double knockout strains and influences on rice disease resistance are studied, and results show that compared with a wild type, a mutant plant is short and small, and the phenotype of the double knockout mutant is more obvious than that of a single knockout mutant; the single knockout strain and the double knockout strain both can improve the resistance of the rice to the xanthomonas oryzae sclerotiorum, and the double knockout strain has stronger resistance, so that a new genetic resource is provided for research on disease-resistant breeding of the rice.

The invention relates to application of a TaGRF2-A gene in enhancing heat resistance of wheat, and belongs to the field of plant genetic engineering. Specifically, the wheat TaGRF2-A gene participating in positive regulation and control of high-temperature stress resistance is cloned in wheat, so that the wheat TaGRF2-A gene is overexpressed, and compared with a wild plant, a wheat plant with the overexpressed TaGRF2-A gene shows higher high-temperature resistance; the TaGRF2-A gene enhances the tolerance of wheat under a high-temperature condition in manners of regulating the stomatal conductance of plants, optimizing the photosynthesis efficiency, improving the water utilization efficiency and the like, and a new genetic engineering modification means is provided for cultivating a new wheat variety with high yield, stable yield and high temperature resistance.

This invention belongs to the field of plant genetic engineering and relates to the application and method of reducing the expression of the Zm7649 gene in improving plant stress resistance. This invention utilizes an EMS mutant with premature termination of expression. Zm7649-ems Heterologous overexpression with Arabidopsis thaliana Zm7649-OE Verified Zm7649 The function of negatively regulating salt stress and high temperature stress was studied, and its preliminary analysis was performed. Zm00001d027649 The molecular mechanism regulating stress involves the interaction between Zm7649 and a maize catalase (Zm4848), with ubiquitination degrading Zm4848 and reducing its protein stability. This invention provides new genetic resources and theoretical basis for maize stress-resistance breeding or germplasm innovation.