72 results about "New genetics" patented technology

The invention relates to the technical field of gene engineering, and discloses an METS2 gene for regulating and controlling the grain shape of rice, a protein and application of the METS2 gene, and the nucleotide sequence of the METS2 gene is shown as SEQ ID NO.1. The invention further discloses application of the METS2 gene in regulation and control of the rice grain shape. According to the METS2 gene for regulating and controlling the rice grain shape, the protein and the application of the METS2 gene, the METS2 gene is overexpressed in a rice plant, short-grain-shaped rice can be obtained, the short-grain-shaped rice is not prone to breakage in the processing process, and the quality of a finished product is high; a basis is provided for further clarification of the molecular mechanism of the rice grain shape, and new gene resources and breeding resources are provided for rice breeding.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a wheat transcription factor TaWRKY22 and a coding gene thereof in regulation and control of plant stripe rust resistance. The function of the wheat transcription factor TaWRKY22 is researched through molecular biology and genetics methods, it is found that knockout of the TaWRKY22 gene significantly improves the resistance of wheat to stripe rust, overexpression of the TaWRKY22 gene reduces the resistance of wheat to stripe rust, and then it is indicated that the wheat transcription factor TaWRKY22 plays a negative regulation role in wheat stripe rust resistance. Therefore, the TaWRKY22 gene is subjected to targeted modification through a gene editing technology, a new genetic material and a molecular breeding strategy can be provided for wheat stripe rust resistance breeding, and an important material and technical support are provided for breeding of wheat disease-resistant varieties.

The invention belongs to the technical field of biology, and particularly relates to a TaGAT2-1B gene resistant to wheat stripe rust and application of the TaGAT2-1B gene, and the nucleotide sequence of the TaGAT2-1B gene is shown as SEQ ID NO.3. It is found that the TaGAT2-1B gene and the encoding protein thereof can effectively improve the wheat stripe rust resistance, the TaGAT2-1B gene is a new stripe rust resistance gene in the wheat adult-plant stage, infection of various stripe rust strains can be effectively resisted, the field phenotype of the TaGAT2-1B gene shows the characteristic of slow rust, and the TaGAT2-1B gene and the encoding protein thereof have good application prospects. The discovery of the gene provides a new genetic resource for the research of the wheat stripe rust resistance mechanism and the wheat stripe rust resistance breeding.

The invention provides an application of a citrus transcription factor CsAGL9 gene and a protein coded by the citrus transcription factor CsAGL9 gene in regulating and controlling the content of citric acid in citrus fruits, the nucleotide sequence of the CsAGL9 gene is shown as SEQ ID NO.1, and the amino acid sequence of the protein is shown as SEQ ID NO.2. The content of citric acid in the fruits can be reduced through overexpression in the oranges; the content of citric acid in fruits can be increased by silencing the gene. It is further verified that the gene promotes the activity of the citrus citric acid transporter CsTCT1. The discovery provides a new gene resource for citrus quality improvement and molecular design breeding, and provides an efficient tool for fruit quality improvement.

The invention belongs to the technical field of biology, and particularly relates to application of a GmNIC1 gene and a GmNIC2 gene to regulation and control of soybean cyst nematode resistance of plants, and the nucleotide sequence of the GmNIC1 gene is shown as SEQ ID NO.1; the nucleotide sequence of the GmNIC2 gene is as shown in SEQ ID NO. 2. The invention discloses the negative regulation effect of GmNIC1 and GmNIC2 genes in the resistance of soybean to cyst nematode (SCN) for the first time, and by constructing gene silencing mutants of GmNIC1 and GmNIC2, it is verified that the resistance of soybean to SCN can be remarkably enhanced due to function deficiency of the gene family. The application provides a new target for deep understanding of a soybean-nematode interaction mechanism, and provides a brand new gene resource and technical path for molecular design breeding of resistant soybeans.

The invention discloses a chloroplast RNA edited gene OsPPR7 for temperature-sensitive regulation and control of rice and application of the gene OsPPR7, and belongs to the technical field of gene engineering. The CDS sequence of the OsPPR7 gene is as shown in SEQ ID NO. 1. The OsPPR7 is an RNA (Ribonucleic Acid) editing factor which has a bidirectional regulation and control function and is sensitive to temperature. Editing of multiple sites on the rpoB gene is negatively regulated and controlled, and meanwhile RNA editing of multiple genes such as ndhB and ndhD which are crucial to the chloroplast function is positively regulated and controlled under high-temperature stress. Under the stress of high temperature of 35 DEG C, the loss of functions can lead to obvious reduction of RNA editing efficiency of a plurality of ndh genes, and serious yellowing of seedlings is caused. The key function of the rice OsPPR7 gene in regulation and control of rice growth and development and high-temperature response is clarified for the first time, the importance of the OsPPR7 gene in maintenance of chloroplast functions under high-temperature stress is clarified, and a new gene resource and a technical path are provided for cultivation of new varieties of high-temperature-resistant rice.

The invention belongs to the technical field of plant genetic engineering, and particularly discloses application of an important member of a potato mitogen-activated protein kinase (MAPK) family, namely an StMKK9 gene in regulation and control of maturity and cold resistance of potatoes. CDNA of a cultivar Desiree leaf is used as a template, the potato StMKK9 gene is separated and cloned, an over-expression vector is constructed, and the over-expression vector is used for expression of the potato. Genetic transformation of the potatoes is further carried out, it is found that the overexpressed StMKK9 gene can promote prematurity of the potatoes, meanwhile, the cold resistance of potato plants can be improved, and a new genetic material and a theoretical basis are provided for analysis of a potato tuberization molecular mechanism and improved breeding of the potatoes.

The invention discloses an application of an overexpressed CrOASTL1 gene in improving the soluble protein content of leguminous forage, and belongs to the technical field of biology. 1, and the CDS sequence of the CrOASTL1 gene is as shown in SEQ ID NO. 2. The method comprises the following steps: cloning a CrOASTL1 gene segment, constructing a plant overexpression vector, carrying out plant transgenosis operation, and introducing into leguminous forage plant cells to obtain a leguminous forage transgenosis line with the overexpressed CrOASTL1 gene. The CrOASTL1 gene is over-expressed in medicago sativa, and the soluble protein content of leaves of the medicago sativa is remarkably increased by about 19%. The CrOASTL1 gene of chlamydomonas reinhardtii is applied to quality improvement of leguminous forage for the first time, the technical obstacle that the genetic relationship of species is relatively far is overcome, a brand new gene resource and an efficient technical approach are provided for cultivating a new variety of leguminous forage with high protein and high nutritional value, and the method has important significance in promoting sustainable development of grass and animal husbandry.

The invention discloses a gene OsNST2 for transporting nucleotide sugar and application of the gene OsNST2. A nucleotide sequence of a coding region of the gene is as shown in SEQ ID No. 1, and an amino acid sequence coded by the gene is as shown in SEQ ID No. 2. It is found for the first time that the growth, development and yield of rice are affected by the OsNST2 by regulating and controlling formation of rice cell walls, an OsNST2 gene knock-down strain is created through an RNAi technology, an OsNST2 gene overexpression strain is obtained through an overexpression transgenic technology, it is verified that the OsNST2 gene is a new gene for regulating and controlling formation of the rice cell walls, and a new gene resource is provided for high yield of rice.

The invention relates to the technical field of plant genetic engineering, in particular to application of a rice OsERF141 protein and a coding gene thereof in improving low-temperature tolerance of plants. Experiments show that the low-temperature resistance of an OsERF141 function deletion mutant strain is remarkably reduced, and the low-temperature resistance of an overexpressed OsERF141 transgenic strain is remarkably improved. The invention provides a new gene resource for the cold resistance problem of rice, and has important significance for the molecular mechanism research of low-temperature stress and the cultivation of new low-temperature-resistant varieties.

The application discloses a corn kernel size related protein SSRP1 and application thereof. The technical problem solved is how to regulate the size of corn kernels. Specifically disclosed is the application of a protein, a substance for regulating the expression of a coding gene of the protein, or a substance for regulating the activity or content of the protein in regulating the size of plant kernels and / or preparing a product for regulating the size of plant kernels and / or plant breeding and / or preparing a plant breeding product, the protein being a protein as shown in sequence 3 in the sequence listing. The application provides a new genetic resource for the study of the size of plant kernels, in particular corn kernels, and will play an important role in the application in the field of corn seed production and breeding.

The invention relates to the field of plant genetic engineering, in particular to novel application of a soybean ACR gene family member GmACR39 in improving saline-alkaline tolerance of plants. The invention provides application of a separated gene in preparation of a product for improving saline-alkaline tolerance of plants, and the gene encodes a nucleotide sequence shown in SEQ ID NO: 1 or encodes a protein which has at least 80% identity with SEQ ID NO: 2 and has a function of improving saline-alkaline tolerance of plants. The expression of the gene is up-regulated under saline-alkaline stress, and the saline-alkaline tolerance of a transgenic plant can be remarkably improved by over-expression of the gene. The invention also provides an expression vector containing the gene, an sgRNA molecule targeting a promoter of the gene, a CRISPR activation system, and related reagents, kits, a method for cultivating saline-alkaline tolerant plants and detection means. The invention provides a new gene resource and a molecular breeding tool for cultivating new varieties of saline-alkaline tolerant crops.

The application provides a cold-resistant gene TrWRKY10a540, an expression vector, application and breeding method thereof, and belongs to the field of plant molecular genetics and genetic improvement. Specifically disclosed is a cold-resistant gene TrWRKY10a540 and an expression vector derived from Trifolium repens L. and application thereof in improving the cold resistance of plants, and the problem of insufficient research on low-temperature stress related genes of Trifolium repens in the prior art is solved. The application further discloses a breeding method for regulating the cold stress resistance of plants by the cold-resistant gene TrWRKY10a540, and research results show that the gene can significantly improve the cold resistance and antioxidant level of plants under low-temperature stress conditions, and has good functional effects. The application can be widely applied to plant molecular breeding, is particularly suitable for improving the low-temperature resistance of economic crops such as forage grasses, provides a new genetic resource for the breeding of cold-resistant plants, and has important significance for revealing the regulation mechanism of the cold resistance of plants.

The application belongs to the technical field of plant molecular genetic engineering, and particularly relates to a FveDRD1 gene for regulating sugar accumulation in strawberry fruits and application thereof. The sequence of the FveDRD1 gene is shown as SEQ ID NO:1, and the amino acid sequence encoded by the FveDRD1 gene is shown as SEQ ID NO:2. The FveDRD1 gene can positively regulate sugar accumulation, such as sucrose, glucose and fructose, in strawberry fruits. By inhibiting or eliminating the expression of the FveDRD1 gene in the strawberry fruits, the sugar accumulation in the fruits can be reduced, and the sweetness can be reduced. The application provides a new genetic resource for improving the quality of strawberries.

The invention discloses application of a lotus NnXTH2 gene to promotion of dwarfing and compactness of plant types and enhancement of rachis mechanics, and belongs to the technical field of biology. And the NnXTH2 gene is used for coding a protein as shown in SEQ ID NO. 4. The research finds that the rachis mechanics of the transgenic tobacco strain overexpressing the NnXTH2 gene is obviously enhanced, the plant height of the plant is reduced, the internode spacing is shortened, and the plant type of the plant is dwarfed and compact. The result shows that overexpression of the NnXTH2 gene can significantly enhance the rachis mechanics of the plant, shorten the plant height and internode spacing of the plant, make the plant type dwarf and compact, and better meet the plant type and rachis quality requirements of fresh cut flowers. According to the invention, the function of the NnXTH2 gene in regulation and control of plant type and rachis mechanics is defined for the first time, and new genetic resources and theoretical basis are provided for cultivating high-quality cut flower plants with rachis not easy to break and higher ornamental value.

The invention provides a VabHLH016 gene capable of improving cold resistance of plants and application of the VabHLH016 gene, and belongs to the technical field of molecular breeding. The invention provides a VabHLH016 gene derived from vitis amurensis and an application scheme of the VabHLH016 gene, so that the cold resistance of a receptor plant can be effectively improved. The method has the direct effects that the plant treated by the technology shows stronger survival activity and lighter cold injury symptom when encountering a low-temperature environment, and can better maintain a normal physiological state. The invention provides a new genetic resource and technical approach for solving the cultivation limitation of thermophilic crops such as grapes in high latitude or cold regions, and has important application value for breeding new cold-resistant varieties and ensuring stable yield and income increase of fruit tree industry.

The invention discloses a TRIM50 mutant gene of Jingmen green-shell laying hens and application of the TRIM50 mutant gene in regulation and control of cell growth, and belongs to the technical field of gene engineering. According to the invention, a TRIM50 mutant gene is identified in a group of Jingmen green-shell laying hens, and the nucleotide sequence of the TRIM50 mutant gene is shown as SEQ ID NO.4. Compared with a chicken TRIM50 wild type gene in NCBI (National Center of Biotechnology Information), the mutant gene has no obvious influence on cellular morphology and protein localization; however, the mutant gene can significantly promote cell proliferation, improve cell viability and inhibit cell apoptosis. According to the invention, a theoretical basis is provided for research on cell proliferation and apoptosis mechanisms of the Jingmen green-shell laying hens, a technical support is provided for construction of a high-activity cell model, and a new genetic resource is provided for identification of animal growth performance and molecular assisted breeding.

The invention relates to an astaxanthin-producing engineering bacterium as well as a construction method and application thereof. According to the astaxanthin-producing engineering bacterium, a crtE gene, a crtI gene, a crtB gene, a crtY gene, a crtZ gene, a crtW gene, a dxs gene and an idi gene are over-expressed. A brand-new genetic modification strategy is designed and comprises the steps that genes in an astaxanthin synthesis route are integrated to an escherichia coli genome, engineering bacteria for producing the astaxanthin are constructed, supply of astaxanthin synthesis precursors is improved by expressing dxs genes and idi genes on the genome, the yield of the astaxanthin is further increased, in addition, a specific crtW gene source is controlled, and the yield of the astaxanthin is increased. According to the present invention, the gene expression quantities of the crtY, the crtZ and the crtW, the astaxanthin yield can be further improved, the maximum shake flask level yield can achieve 27.4 mg / L, and the gene expression on the chromosome is further beneficial to the amplification to the industrial production.

The invention discloses an SNP molecular marker linked with the sucrose content of peanut seed kernels, a KASP primer group and application of the SNP molecular marker and the KASP primer group, and belongs to the technical field of molecular genetic breeding. The molecular marker SucB06 related to the sucrose content of the peanut seed kernels is screened, a KASP primer group is developed according to the molecular marker and the SucA06 molecular marker, a peanut variety with the high sucrose content of the seed kernels can be accurately distinguished from a peanut variety with the low sucrose content of the seed kernels by utilizing a KASP genetic typing technology, and the peanut variety with the low sucrose content of the seed kernels can be accurately distinguished. The method can be used for early identification and screening of peanut materials, the peanut breeding period can be shortened, the genetic resource improvement process is accelerated, and new genetic resources and theoretical basis are provided for molecular assisted breeding.

The invention discloses application of an ASF1 gene to improvement of plant disease resistance or cultivation of disease-resistant plants, and belongs to the technical field of gene engineering. The ASF1 gene comprises an ASF1A gene and an ASF1B gene, the nucleotide sequence of the ASF1A gene is shown as SEQ ID NO. 1, and the nucleotide sequence of the ASF1B gene is shown as SEQ ID NO. 2. Phenotypes of ASF1A single knockout strains, ASF1B single knockout strains, ASF1A double knockout strains and ASF1B double knockout strains and influences on rice disease resistance are studied, and results show that compared with a wild type, a mutant plant is short and small, and the phenotype of the double knockout mutant is more obvious than that of a single knockout mutant; the single knockout strain and the double knockout strain both can improve the resistance of the rice to the xanthomonas oryzae sclerotiorum, and the double knockout strain has stronger resistance, so that a new genetic resource is provided for research on disease-resistant breeding of the rice.

The invention relates to application of a TaGRF2-A gene in enhancing heat resistance of wheat, and belongs to the field of plant genetic engineering. Specifically, the wheat TaGRF2-A gene participating in positive regulation and control of high-temperature stress resistance is cloned in wheat, so that the wheat TaGRF2-A gene is overexpressed, and compared with a wild plant, a wheat plant with the overexpressed TaGRF2-A gene shows higher high-temperature resistance; the TaGRF2-A gene enhances the tolerance of wheat under a high-temperature condition in manners of regulating the stomatal conductance of plants, optimizing the photosynthesis efficiency, improving the water utilization efficiency and the like, and a new genetic engineering modification means is provided for cultivating a new wheat variety with high yield, stable yield and high temperature resistance.

This invention belongs to the field of plant genetic engineering and relates to the application and method of reducing the expression of the Zm7649 gene in improving plant stress resistance. This invention utilizes an EMS mutant with premature termination of expression. Zm7649-ems Heterologous overexpression with Arabidopsis thaliana Zm7649-OE Verified Zm7649 The function of negatively regulating salt stress and high temperature stress was studied, and its preliminary analysis was performed. Zm00001d027649 The molecular mechanism regulating stress involves the interaction between Zm7649 and a maize catalase (Zm4848), with ubiquitination degrading Zm4848 and reducing its protein stability. This invention provides new genetic resources and theoretical basis for maize stress-resistance breeding or germplasm innovation.

This invention discloses overexpression cysK The application of genes in increasing the soluble protein content of leguminous forage grasses belongs to the field of biotechnology. cysK The CDS sequence of the gene is shown in SEQ ID NO. 2. A plant overexpression vector containing the nucleotide sequence shown in SEQ ID NO. 2 was constructed, and the plant overexpression vector was introduced into leguminous forage plant cells to obtain overexpressed genes. cysK Transgenic leguminous forage lines with overexpressed genes in alfalfa. cysK The gene significantly increases the soluble protein content in its leaves by approximately 28%. This invention is the first to utilize Escherichia coli derived from prokaryotes. cysK The successful application of genes to the quality improvement of leguminous forage grasses has overcome the technical barrier of distant species kinship, providing new genetic resources and efficient technical approaches for breeding new varieties of leguminous forage grasses with high protein and high nutritional value, which is of great significance for promoting the sustainable development of the grass animal husbandry industry.

Provided are cells that are resistant to mobile genetic elements or horizontal gene transfer, and methods for obtaining said cells. Also provided are methods for preventing the horizontal transfer of genetic information between a mobile genetic element and a first cell, cells making use of new genetic codons schemes and related subject matter, kits comprising mutually orthogonal cells, and mobile genetic elements. Also provided are methods of altering the susceptibility of a gene to mutations that alter the encoded amino acid sequence, methods for evolving or improving a protein, and methods for rendering a target gene more resistant to mutation. Additionally provided are uses of the cells for making polymers and methods comprising using the cells for making polymers.

This invention discloses a ZmLG2 gene mutant and its application in regulating maize root architecture and nitrogen utilization efficiency, relating to the field of biotechnology. The ZmLG2 gene mutant has the DNA sequence shown in SEQ ID No. 3. Compared with the wild-type ZmLG2 gene SEQ ID No. 1, it contains the following mutations: a 21-base deletion at the end of the first exon CDS1; a complete deletion of the first intron intron1; a 189-base deletion at the beginning of the second exon CDS2; a T→G mutation at +2284, a T→C mutation at +2337, a G→A mutation at +2344, a C→A mutation at +2361, and a C→A mutation at +2394 downstream of the DNA start site; and a 1-base deletion at +2838. The ZmLG2 mutant plays a key role in regulating maize root architecture and improving nitrate absorption and utilization efficiency. It has significant biological functions and application value, providing new genetic resources and technical support for breeding crop varieties with high nitrogen fertilizer utilization efficiency. It also has broad application prospects in promoting green and sustainable agricultural development.

The invention discloses an application of an AP2 transcription factor gene Chalk8 in regulation and control of chalkiness traits of rice, a nucleotide sequence of a Chalk8 gene coding region is shown as SEQ ID NO.1, an amino acid sequence is shown as SEQ ID NO.2, and the chalkiness traits comprise chalkiness grain rate and chalkiness degree. According to the invention, the AP2 transcription factor Chalk8 is found to be a key gene for influencing chalkiness difference between different rice varieties through a whole genome association analysis method for the first time. A gene editing technology is further utilized to create a Chalk8 gene knockout mutant, a Chalk8 gene overexpression strain is obtained through a transgenic technology, and a novel method for reducing chalkiness of rice by enhancing expression of the Chalk8 gene is verified. Besides, a Chalk8 allele related to chalkiness variation between rice varieties is identified through haplotype analysis, the Chalk8 allele comprises an excellent haplotype capable of remarkably reducing chalkiness, and a new gene resource is provided for rice quality improvement.

This invention discloses a mutant TRIM50 gene in Jingmen green-shelled laying hens and its application in regulating cell growth, belonging to the field of genetic engineering technology. This invention identified a mutant TRIM50 gene in a Jingmen green-shelled laying hen population, its nucleotide sequence of which is shown in SEQ ID NO.4. Compared to the wild-type chicken TRIM50 gene in NCBI, this mutant gene has no significant effect on cell morphology and protein localization; however, it significantly promotes cell proliferation, increases cell viability, and inhibits apoptosis. This invention provides a theoretical basis for studying the mechanisms of cell proliferation and apoptosis in Jingmen green-shelled laying hens, provides technical support for constructing high-viability cell models, and provides new genetic resources for identifying animal growth performance and molecular-assisted breeding.

This invention discloses oats AsNF-YA14 The application of genes and their encoded proteins belongs to the field of bioengineering technology. This invention discloses for the first time the properties of oats... AsNF-YA14 A novel function of genes in regulating plant flowering time. Experiments show that... AsNF-YA14 The gene is highly expressed in oat spikelets, and its expression level gradually increases with the growth process, suggesting that it may be involved in flowering regulation. Overexpression in Arabidopsis thaliana... AsNF-YA14 The transgenic plants exhibited a significant early-flowering phenotype, blooming approximately 11 days earlier than the wild-type plants, while also showing a significant reduction in the number of rosette leaves. These results indicate that... AsNF-YA14 Genes have the function of promoting earlier flowering in plants. This invention provides new genetic resources for the genetic improvement of plant flowering time, which can be used to breed new varieties of oats or other crops with early flowering and short growth periods. This is of great significance for expanding crop planting areas, increasing the multiple cropping index, and achieving stable and high yields.

The application belongs to the technical field of genetic engineering and plant disease control, and particularly relates to a gene for positively regulating plant disease resistance and application thereof. The gene is named SIT7, the nucleotide sequence of which is shown as SEQ ID NO. 1, and the amino acid sequence encoded by the gene is shown as SEQ ID NO. 2. The application proves that overexpression of the gene in plants can significantly enhance the disease resistance of the plants, which is manifested by the increase of SA accumulation in the plants and the increase of the expression level of defense-related genes, indicating that SIT7 is involved in the SA-regulated plant immune process. The application provides a brand-new genetic resource for genetic improvement of plant disease resistance, and has a good application prospect.

The invention relates to the field of plant genetic engineering, in particular to an MMR1 protein for regulating and controlling ROS homeostasis of a plant root tip meristematic region as well as a coding gene and application of the MMR1 protein. The method comprises the following steps: knocking out an MMR1 gene to obtain stable transgenic lines mmr1-1 and mmr1-2 of arabidopsis thaliana, and constructing an MMR1 gene complement line and an MMRI gene overexpression line; compared with a wild type, the mutants mmr1-1 and mmr1-2 show that the root length is shortened, the root tip meristematic region is shortened, and the root tip division capability is weakened; the back-complemented strain shows that the MMR1 protein is positioned in mitochondria; the root of an overexpressed strain is longer than that of a wild type, which shows that the MMR1 gene plays an important role in the root elongation of arabidopsis thaliana, the development of a root tip meristematic zone and the cell viability of the root tip meristematic zone. According to the invention, the blank in the field of mitochondrial localization protein regulation and control of root development and ROS homeostasis is filled up, and a new genetic gene resource is provided for root improvement and transgenic breeding.