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Genetic marker for porcine litter size character and application of genetic marker

A technology of genetic markers and litter size, applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc.

Active Publication Date: 2015-01-28
INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the effect of porcine STIM1 gene on sow litter size has not been reported

Method used

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  • Genetic marker for porcine litter size character and application of genetic marker
  • Genetic marker for porcine litter size character and application of genetic marker
  • Genetic marker for porcine litter size character and application of genetic marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Obtaining of porcine STIM1 gene fragment and establishment of polymorphism detection method

[0024] 1. Extraction of pig genomic DNA

[0025] The test pig breeds of the present invention are large white pigs, Meishan pigs and DVI pigs (the samples come from the Institute of Animal Husbandry and Veterinary Medicine of the Hubei Academy of Agricultural Sciences and Huazhong Agricultural University, the Large White pigs are foreign blood-related pigs, and the Meishan pigs are Chinese local pig blood-related pigs. , DVI pig is a new breed of large white pig and Tongcheng pig crossbreeding). The extraction of pig genomic DNA adopts the genomic DNA kit (operated according to the instructions of the kit) produced by Beijing Biotech Biotechnology Co., Ltd., and the specific steps are as follows:

[0026] (1) Take pig ear or tail tissue, put it into a 2ml centrifuge tube, add 200μl lysate TL, and blow it evenly with the tip of the pipette;

[0027] (2) Add 20 μl of ...

Embodiment 2

[0048] Example 2 The polymorphic distribution detection application of the genetic markers prepared by the present invention in different pig herds

[0049] The applicant detected the distribution frequencies of the two alleles of the pig STIM1 gene in three populations of Large White pigs, DVI lines, and Meishan pigs. Low, only 18.97%, 40.19% in the DVI line, the highest in Meishan pigs, up to 56.25%. Among the three groups, the average litter sizes per litter of Large White pigs, DVI line and Meishan pigs were 10.99±1.95, 11.67±1.77 and 15.21±3.14, respectively. It can be seen that the higher the litter size, the higher the N allele frequency.

[0050] Table 1 Distribution of two alleles of porcine STIM1 gene in different populations

[0051]

Embodiment 3

[0052] Example 3 Association analysis and application of genetic markers prepared by the present invention and pig litter size traits

[0053] The applicant recorded reproductive traits in 174 Large White pig herds (from the Institute of Animal Husbandry and Veterinary Medicine, Hubei Academy of Agricultural Sciences) and 107 Chinese lean pig new line DIV (from Huazhong Agricultural University) and a total of 726 parities The relationship between the genetic markers prepared by the present invention and the litter size traits of pigs was analyzed. The PCR direct sequencing method established in Example 1 was used for genotyping detection, and the SPSS statistical software (Statistical Package for the Social Sciences, Version 17.0) general linear model GLM was used for statistical analysis, wherein the fixed effects included field number, year and calving season effect.

[0054] The results of the correlation analysis are summarized in Table 2. It can be seen from the table th...

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Abstract

The invention belongs to the technical field of the preparation of livestock molecular genetic markers and particularly relates to a genetic marker for a porcine litter size character and an application of the genetic marker. A T base insertion / deletion mutation of a tenth intron tail end sequence of a porcine STIM1 gene is taken as a genetic marker related to the porcine litter size character. According to the genetic marker provided by the invention, the genetic marker related to the porcine litter size character is obtained through preparation, wherein a nucleotide sequence of the genetic marker of a Yorkshire pig is as shown in SEQ ID NO:1, and a T base insertion mutation exists at 256bp of the sequence; a nucleotide sequence of the genetic marker of a Meishan pig is as shown in SEQ ID NO:2, and a T base deletion mutation exists at 256bp of the sequence; and the mutations can obviously influence the porcine litter size character. The invention further discloses a preparation method for the genetic marker and an application of the genetic marker to the association analysis of the porcine litter size character. A new genetic marker is provided for the molecular marker auxiliary breeding of the porcine litter size character.

Description

technical field [0001] The invention belongs to the technical field of livestock molecular marker (genetic marker) preparation, and in particular relates to a genetic marker and application of pig litter size traits. The genetic marker is cloned from porcine STIM1 gene. Background technique [0002] The litter size of pigs is an important economic trait among reproductive traits, which is directly related to the economic benefits of the pig industry. Increasing the litter size of pigs is of great significance to improve the overall economic benefits of the pig industry. According to statistics, in my country, if the litter size of sows is increased by 1 head per litter, net profit of RMB 19 billion will be added each year (Wang Lixing and Lu Shaoxiong, 2008). The litter size trait of sows is a compound trait composed of several component traits with chronological characteristics such as ovulation, fertilization, implantation, and fetal development. Breeding technology is u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 吴俊静梅书棋彭先文乔木武华玉刘贵生李凤娥宋忠旭孙华李良华李明波董斌科雷彬陶虎
Owner INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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