Culture medium composition suitable for germ-free germination of orchid seeds and method thereof

A kind of germination medium, the technology of culture medium, is applied in the field of aseptic germination medium composition suitable for Cymbidium cymbidium seeds, can solve long germination period (half a year to more than one year, can't realize large-scale factory production, does not disclose improved MS Formula and other issues, to achieve the effect of improving breeding efficiency, saving rare and endangered plants, and shortening the breeding cycle

Inactive Publication Date: 2010-01-13
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Application Information

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Problems solved by technology

However, in the research on the aseptic germination of Cymbidium seeds in the above article, it is only roughly mentioned that the selected basic medium is improved MS, and the specific formula for improving MS is not disclosed; The method is more cumbersome to operate and can easily cause pollution
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  • Culture medium composition suitable for germ-free germination of orchid seeds and method thereof

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Embodiment 1

[0023] Embodiment 1 (being suitable for Cymbidium seed aseptic sowing optimization medium and rapid germination method 1)

[0024] 1) The preparation of the medium, including the content of basic medium and seed germination medium additives:

[0025] (1) Basic medium: self-improved MS, in which sucrose is 20-30g / L, agar is 6g / L, and pH is 5.2;

[0026] (2) Germination medium: basic medium+6-BA1.0mg / L+NAA 1.0mg / L+activated carbon 1g / L+coconut juice 50ml / L;

[0027] 2) Selection of treatment objects: take the uncracked capsules that have grown for 6 months after pollination as treatment objects;

[0028] 3) Pretreatment of the treatment object: the treatment object was stored in the micro-freezing layer of the refrigerator for 10 days at 1-3°C.

[0029] 4) Aseptic seeding of the treated object: take it out from the refrigerator, rinse it with detergent and tap water, soak it in 75% alcohol for 3.0 minutes under sterile conditions, then sterilize it with 0.1% mercury liter solu...

Embodiment 2

[0032] Embodiment 2 (suitable for Cymbidium seed aseptic sowing optimization medium and rapid germination method 2)

[0033] 1) The preparation of the medium, including the content of basic medium and seed germination medium additives:

[0034] (1) Basic medium: improved MS, in which sucrose is 30g / L, agar is 5g / L, and pH is 5.6;

[0035] (2) Germination medium: basic medium + 6-BA0.5mg / L + NAA 0.5mg / L + activated carbon 1g / L + coconut milk 100ml / L;

[0036] 2) Selection of treatment objects: take the uncracked capsules that have grown for 7 months after pollination as treatment objects;

[0037] 3) Pretreatment of the treatment object: the treatment object was stored in the micro-freezing layer of the refrigerator for 10 days at 1-3°C.

[0038] 4) Aseptic seeding of the treated object: take it out from the refrigerator, rinse it with detergent and tap water, soak it in 75% alcohol for 3.0 minutes under sterile conditions, then sterilize it with 0.1% mercury liter solution f...

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Abstract

The invention mainly relates to a culture medium composition suitable for germ-free germination of orchid seeds and a method thereof. Various components of a basic culture medium and a seed germination culture medium are as follows: 500mg/L of calcium nitrate, 200mg/L of potassium chloride, 20-30g/L of sucrose or white sugar and 5-7g/L of agar, wherein the basic culture medium is selected from self-improved MS, i.e. on the basis of the original MS culture medium, 440mg/L of calcium chloride in the MS basic culture medium is replaced into 500mg/L of calcium nitrate and 200mg/L of potassium chloride; and the pH value is 5.0-5.6. The seed germination culture medium comprises the basic culture medium, 0.5-1.0mg/L of 6-BA, 0.5-1.0mg/L of NAA, 1.0g/l of active carbon and 50-100m/L of coconut milk. The invention has the advantages that: (1) the orchid group-cultured optimized culture medium has strong pertinence, good adaptability and high seed germination rate; (2) the method greatly shortens the breeding period of orchid so as to rapidly obtain new genetic improvement; and (3) the method is convenient for operation and reduces the pollution to the minimum extent.

Description

technical field [0001] The invention relates to the technical field of aseptic sowing of plant seeds, in particular to a suitable aseptic germination medium composition for Cymbidium seeds and a method thereof. Optimal medium formula for aseptic germination of Cymbidium seeds and a method for promoting germination. Background technique [0002] Cymbidium faberi, also known as Xiahui, Jiujielan, etc., is a large species of orchids in China. Because of its strong fragrance, beautiful flower shape and rare famous products, it is regarded as a high-end potted flower in Southeast Asia. Cymbidium grows naturally in hillside forests, and most of the cultivated varieties are selected from the natural variation of wild species. Due to the long-term indiscriminate digging by humans and the continuous deterioration of the living environment of orchids, coupled with their extremely slow reproduction speed, the resources of wild Cymbidium in my country are becoming increasingly scarce,...

Claims

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Application Information

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IPC IPC(8): A01G31/00A01G9/10A01C1/00
Inventor 孙崇波郭方其黎侠林森洪秦德辉张姗姗
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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