Polymorphism of MDM2 gene related to occurrence of nasopharyngeal carcinoma and lymphatic metastasis and detection method thereof

A technology for lymph node metastasis and MDM2, applied in the field of detection kits for the above-mentioned susceptible genotypes, can solve problems such as the role that has not been clearly studied, and achieve the effect of reducing the risk of disease and more serious lymph node metastasis.

Inactive Publication Date: 2008-11-05
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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Problems solved by technology

However, the role of SNP309 ...

Method used

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  • Polymorphism of MDM2 gene related to occurrence of nasopharyngeal carcinoma and lymphatic metastasis and detection method thereof
  • Polymorphism of MDM2 gene related to occurrence of nasopharyngeal carcinoma and lymphatic metastasis and detection method thereof
  • Polymorphism of MDM2 gene related to occurrence of nasopharyngeal carcinoma and lymphatic metastasis and detection method thereof

Examples

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Embodiment 1

[0036] In this example, a set of primer pairs was designed and synthesized, and on the basis of the primer pairs, a method for detecting the SNP309 polymorphism site of the MDM2 gene associated with the risk of nasopharyngeal carcinoma and more serious lymph node metastasis was designed .

[0037] 1. Extraction of genomic DNA:

[0038] The phenol / chloroform method in this field is used to extract genomic DNA from peripheral blood leukocytes.

[0039] 1) Take sodium citrate or EDTA-Na 2 Anticoagulant whole blood 5ml (try not to use heparin for anticoagulation), placed in a 50ml centrifuge tube with a lid;

[0040] 2) Add 3-5 times the volume of cold distilled water, reverse and mix repeatedly, place in ice for 5 minutes, and centrifuge at 4°C at 2000 rpm for 20 minutes;

[0041] 3) Pour the supernatant slowly, add pre-chilled 0.1% Triton-X100 (the same volume as above) to the pellet, gently mix the pellet, centrifuge as above, and discard the supernatant;

[0042] 4) Add 4ml of lys...

Embodiment 2

[0054] This example uses the method established in Example 1 to analyze the SNP309 of the MDM2 gene in two populations (Guangxi population: 593 nasopharyngeal cancer patients and 480 controls; Guangdong population: 239 nasopharyngeal cancer patients and 286 controls). The distribution of locus genotypes was analyzed.

[0055] 1. Test object:

[0056] This study included two nasopharyngeal carcinoma case-control populations whose residences were Guangxi Zhuang Autonomous Region and Guangdong Province in southern China. The Guangxi population includes 593 nasopharyngeal cancer patients and 480 control individuals. All individuals are genetically unrelated Chinese living in Nanning and surrounding areas. Nasopharyngeal cancer patients were continuously collected from Guangxi Cancer Hospital (Nanning, China) from September 2003 to July 2005. They were all newly diagnosed and confirmed pathologically, and did not have other types of tumors. The patient's response rate is 95%. Tumor sta...

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Abstract

The invention discloses an MDM2 gene which is a novel predisposing gene of nasopharyngeal darcinoma by relating the SNP 309 polymorphic loci of the MDM2 gene with the risk of the nasopharyngeal darcinoma and the more serious lymph node metastasis, thereby providing new genetic counseling contents and the molecule criterion for people to prevent and control the nasopharyngeal darcinoma. In addition, the invention also relates to a method for detecting the SNP309 polymorphic loci and the corresponding detecting kit, wherein, the detecting method is the PCR product direct sequencing method that is the polymerase chain reaction-sequencing method.

Description

Technical field [0001] The present invention relates to individual susceptibility in the prevention of nasopharyngeal cancer population and its genetic detection method. Specifically, the present invention relates to the genotype of the SNP309 polymorphism site of the susceptibility gene MDM2 related to the susceptibility of nasopharyngeal carcinoma and its detection method. The present invention further relates to a detection kit for the above-mentioned susceptible genotype, and application of the kit. In addition, the present invention also relates to the application of the above detection method and susceptibility gene in the prevention and control of nasopharyngeal carcinoma and related genetic counseling. Background technique [0002] Nasopharyngeal carcinoma (NPC) is a malignant tumor of epithelial origin, and its incidence has obvious regional and ethnic differences. It is relatively high in southern China and Southeast Asian countries or regions (such as Singapore, Taiwan...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 贺福初周钢桥翟芸张小爱张红星
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
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