Cabbage type rape low erucic acid molecular marker, preparation method and application thereof

Abstract

The invention belongs to the rape molecular breeding field, which relates to a codominant SNP molecular marker closely linked with the content of a cabbage type rape erucic acid, a preparation method thereof and application in auxiliary selection of the low erucic acid cabbage type rape. A primer is used to amplify the genome DNA of the low erucic acid cabbage type rape and a high erucic acid cabbage type rape in order to obtain two amplified DNA fragments, and then cloning, sequencing and sequence alignment are carried out on the DNA fragments; according to the single nucleotide polymorphism of mutations 845C-845T in SEQ ID NO: 1 and SEQ ID NO:2, second bases at the 3' ends of forward and reverse primers are simultaneously used to carry out mismatch processing, and a primer pair is designed according to the sequence difference of SEQ ID NO: 3 and SEQ ID NO: 4; the primer pair is validated by PCR amplification and tested by group detection and effect analysis to obtain the codominant SNP molecular marker closely linked with the cabbage type rape low erucic acid gene. The invention can be applied to the development and detection of a cabbage type rape SNP polymorphic marker and the development of SNP markers by using forward and reverse primers simultaneously mismatched in allopolyploid crops such as the cabbage type rape.

Description

technical field [0001] The invention belongs to the technical field of rapeseed molecular breeding, in particular to the preparation and application of a low erucic acid molecular marker of Brassica napus. The molecular markers can be used as marker-assisted selection for canola rape breeding, and provide new molecular markers for cultivating genetically stable new varieties of canola. Background technique [0002] Erucic acid is a 22-carbon long-chain fatty acid with the molecular formula CH 3 (CH 2 ) 7 CH=CH(CH 2 ) 11 COOH. It mainly exists in the seeds of Cruciferae (Cruciferae) and Tropaeolaceae (Tropaeolaceae). The erucic acid content in the seeds of Brassica spp. and Erysimum spp. plants in the Brassicaceae family is generally 30% to 60%, and the erucic acid content in the seeds of Trollaceae plants can be as high as 80%. Erucic acid is also found in the oils of marine animals. Like other fatty acids, erucic acid can mostly be digested and absorbed and metaboli...

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Problems solved by technology

Although the contribution rate of the two loci to the content of erucic acid is still controversial, it is agreed that there are two loci in Brassica napus that control the synthesis of erucic acid and show an additive effect (Fourmann et al., The two genes homologous to Arabidopsis FAE1 co-segregate with the two loci governing erucic acid content in Brassica napus. Theor Appl Genet, 1998, 96: 852-858)

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