Molecular marker closely linked with oil content QTL of cabbage type rape

A cabbage type rapeseed, molecular marker technology, applied in the direction of microbial determination/inspection, DNA/RNA fragmentation, recombinant DNA technology, etc. Effect

Active Publication Date: 2016-04-20
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the synthesis pathway of triacylglycerol (the main storage form of oil) is well understood, we still lack in-depth understanding of the genetic and molecular mechanisms of oil content variation in oil crops, including rapeseed

Method used

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  • Molecular marker closely linked with oil content QTL of cabbage type rape
  • Molecular marker closely linked with oil content QTL of cabbage type rape
  • Molecular marker closely linked with oil content QTL of cabbage type rape

Examples

Experimental program
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Effect test

Embodiment 1

[0039] 1. Construction and field experiment of oil content positioning population of Brassica napus

[0040]In this example, the cabbage rape variety Zhongshuang No. 11 (in charge of the Ministry of Agriculture of China, approved by the National Crop Variety Appraisal Committee, document number: National Examination Oil 2008030; the oil content measured in 2013 was 51.4%) was used as the female parent, With low oil content Brassica napus strain A254 (the oil content measured in 2013 is 45.3%, the source of this material is: China Invention Patent Authorization; Patentee: Huazhong Agricultural University; Patent No. ZL2009102734352; Invention Name: Brassica napus High oleic acid molecular marker and preparation method and application; publication number CN101824472A; publication date 2010.09.08) as male parent hybridization to obtain F1, using the pollen of the F1 plant to be cultured by microspores (for the method of microspore culture, refer to the literature, Yu Fengqun, etc....

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Abstract

The invention belongs to the technical field of rape molecular marker preparation, and particularly relates to a molecular marker closely linked with the oil content QTL of cabbage type rape. The novel molecular marker OilA5 which is closely linked with the oil content QTL and can be used independently or/and used in combination is obtained through screening, and the nucleotide sequence of the molecular marker is shown in the sequence table SEQ ID NO:5 or 6. A primer pair OilA5-A and a primer pair OilA5-G for detecting the molecular marker OilA5 are constructed, and the nucleotide sequences of the primer pairs are shown in the sequence tables SEQ ID NO:1-4. The molecular marker can be applied to molecular marker auxiliary selection in cabbage type rape oil content character improvement and fine mapping and map-based cloning of oil content character sites.

Description

technical field [0001] The invention belongs to the technical field of rapeseed molecular marker preparation, in particular to a molecular marker closely linked to the oil content QTL of Brassica napus, the molecular marker or a combination thereof can be used for marker-assisted selection in the improvement of oil content traits of Brassica napus Quantitative trait locus fine-mapping and map-based cloning applications. Background technique [0002] As one of the most important oil crops in the world, rapeseed can produce about 26 million tons of vegetable oil every year, accounting for about 15% of the total vegetable oil production. The main purpose of rapeseed production is to harvest rapeseed and press it into oil, and increasing its oil yield is the ultimate goal of rapeseed production. The oil yield of rapeseed depends on two factors: rapeseed yield and rapeseed oil content. Compared with the rapid growth of production in the past few decades, the oil content of new ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
CPCC12Q1/6895C12Q2600/13
Inventor 周永明刘晟范楚川杨庆勇
Owner HUAZHONG AGRI UNIV
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