Breeding method for silkworm variety whose secreted fibroin fibril contains sericin protein

A protein and variety technology, applied in animal husbandry, etc., can solve the problems of short growth period, not meeting the practical needs of production, and inability to produce efficiently, and achieve the effect of improving the short growth period

Active Publication Date: 2017-12-19
SUZHOU UNIV
View PDF6 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The transgenic mutant with highly efficient expression of sericin in the rear silk gland obtained by using the above patented technology, although it is a mutant homozygous silkworm, has various excellent cocoon silk production such as silk quantity, fineness, clarity, cocoon color, etc. The potential of traits, but it does not meet the production practical needs of modern sericulture. It is a genetic resource called material in the field of silkworm breeding. The mutant retains the non-diapause and short growth period of the wild-type original variety suitable for transgenic operations. , low amount of cocoon silk, poor development uniformity, etc. Cocoon silk quali...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Breeding method for silkworm variety whose secreted fibroin fibril contains sericin protein
  • Breeding method for silkworm variety whose secreted fibroin fibril contains sericin protein
  • Breeding method for silkworm variety whose secreted fibroin fibril contains sericin protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Breeding of yellow cocoon hybrids with high expression of sericin in the posterior silk gland

[0036] Present embodiment 1 comprises the following steps:

[0037] (1) The Japanese system N4 variety that synthesizes and secretes sericin in the posterior silk gland is prepared according to the method of Chinese patent application 201610348866.0 to obtain a transgenic mutant. The female moth of the mutant is used as the female parent, and the yellow cocoon-bearing moth is used as the female parent. The male moths of the Japanese system M in the dimorphic practical hybrid combination Mzhong×Mri (name of silkworm variety, Caicoon No. 1) mate, and a single moth lays eggs to obtain the first generation of silkworm eggs;

[0038] (2) Raise the hatched larvae of the first generation of silkworm eggs with the method of single moth breeding, take the individual with cocoon silk as green fluorescence as the positive indicator, select the moth area with more than 90% positive indiv...

Embodiment 2

[0058] Breeding of white cocoon hybrids with high expression of sericin in the posterior silk gland

[0059] Present embodiment 2 comprises the following steps:

[0060](1) The female moth of the transgenic mutant of the Japanese system N4 variety (prepared by the method of Chinese patent application 201610348866.0 by using the Japanese system N4 variety) that synthesizes and secretes sericin from the posterior silk glands is used as the female parent, and forms a white cocoon with the female moth The Japanese system 9206 male moths of the dimorphic practical hybrid combination 9205×9206 mate, and a single moth lays eggs to obtain the first generation of silkworm eggs;

[0061] (2) Raise the hatched larvae of the first generation of silkworm eggs with the method of single moth breeding, take the individual with cocoon silk as green fluorescence as the positive indicator, select the moth area with more than 90% positive individuals, and further optimize the positive individual ...

Embodiment 3

[0081] Breeding of special yellow cocoon high-silk hybrid varieties with high-efficiency expression of sericin in the posterior silk gland

[0082] Present embodiment 3 comprises the following steps:

[0083] (1) The female moth of the Japanese system N4 variety transgenic mutant (prepared by the method of Chinese patent application 201610348866.0 by using the Japanese system N4 variety) that synthesizes and secretes sericin from the posterior silk glands is used as the female parent, and has yellow cocoons The dimorphic practical hybridization combination silkworm variety Mzhong×Mday Japanese system Mday male moth mating, single moth lays eggs, and obtains the first generation of silkworm eggs;

[0084] (2) Raise the larvae hatched by the first-generation eggs with the method of raising single moths, and the individuals with cocoon silk showing green fluorescence are positive indicators, and select the moth districts with more than 90% positive individuals, and further optimi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a breeding method for a silkworm variety whose secreted fibroin fibril contains sericin protein. The method comprises the following steps: using female moths of Japanese system variety transgenosis mutants which synthesize and secrete sericin by rear part sericteriums as female parents, mating with male months of a Japanese system silkworm variety of a digoneueism hybridization combination, further using selfing, backcross, marker gene tracking and other methods to breed a Japanese system protospecies whose rear part sericteriums efficiently express sericin; directly hybridizing the bred Japanese system protospecies with a Chinese system protospecies in the above digoneueism hybridization combination, to breed a silkworm hybridization combination variety whose secreted fibroin fibril contains sericin protein. The method can efficiently and rapidly breed the silkworm whose secreted fibroin fibril contains sericin protein by the silkworm hybrid variety which produces common silkworm cocoons, so as to obtain novel silk fibers whose fiber property is changed. The method is suitable for commercialized breeding requirement.

Description

technical field [0001] The invention relates to the field of silkworm variety breeding for special purposes, in particular to a silkworm variety breeding method capable of efficiently and quickly breeding common cocoon-producing silkworm hybrid varieties into mutant silkworm varieties containing sericin protein in the produced silk fibroin fibers. Background technique [0002] The silkworm is a silk insect that has been used for thousands of years. The silk fiber produced by the cocoon silk produced by the mature larvae of the silkworm is known as the queen of fibers. The silk gland of silkworm is a specialized organ for synthesizing and secreting silk protein, and it is the main target of human domestication, selection and transformation of silkworm. The protein synthesis and secretion function of the silk gland of silkworm determines the yield and quality of silkworm cocoons, and fundamentally affects the main yield and quality level of the whole silk industry. Improving ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01K67/04
CPCA01K67/04
Inventor 徐世清王玉军司马杨虎陈息林陈学冬王永锋李秋颖陶卉邢瑞殷为民董慧玲杜贝贝
Owner SUZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap