Breeding method for silkworm variety whose secreted fibroin fibril contains sericin protein

Abstract

The invention provides a breeding method for a silkworm variety whose secreted fibroin fibril contains sericin protein. The method comprises the following steps: using female moths of Japanese system variety transgenosis mutants which synthesize and secrete sericin by rear part sericteriums as female parents, mating with male months of a Japanese system silkworm variety of a digoneueism hybridization combination, further using selfing, backcross, marker gene tracking and other methods to breed a Japanese system protospecies whose rear part sericteriums efficiently express sericin; directly hybridizing the bred Japanese system protospecies with a Chinese system protospecies in the above digoneueism hybridization combination, to breed a silkworm hybridization combination variety whose secreted fibroin fibril contains sericin protein. The method can efficiently and rapidly breed the silkworm whose secreted fibroin fibril contains sericin protein by the silkworm hybrid variety which produces common silkworm cocoons, so as to obtain novel silk fibers whose fiber property is changed. The method is suitable for commercialized breeding requirement.

Description

technical field [0001] The invention relates to the field of silkworm variety breeding for special purposes, in particular to a silkworm variety breeding method capable of efficiently and quickly breeding common cocoon-producing silkworm hybrid varieties into mutant silkworm varieties containing sericin protein in the produced silk fibroin fibers. Background technique [0002] The silkworm is a silk insect that has been used for thousands of years. The silk fiber produced by the cocoon silk produced by the mature larvae of the silkworm is known as the queen of fibers. The silk gland of silkworm is a specialized organ for synthesizing and secreting silk protein, and it is the main target of human domestication, selection and transformation of silkworm. The protein synthesis and secretion function of the silk gland of silkworm determines the yield and quality of silkworm cocoons, and fundamentally affects the main yield and quality level of the whole silk industry. Improving ...

AI Technical Summary

Problems solved by technology

The transgenic mutant with highly efficient expression of sericin in the rear silk gland obtained by using the above patented technology, although it is a mutant homozygous silkworm, has various excellent cocoon silk production such as silk quantity, fineness, clarity, cocoon color, etc. The potential of traits, but it does not meet the production practical needs of modern sericulture. It is a genetic resource called material in the field of silkworm breeding. The mutant retains the non-diapause and short growth period of the wild-type original variety suitable for transgenic operations. , low amount of cocoon silk, poor development uniformity, etc. Cocoon silk quality, such as cocoon silk fineness, clarity and strength, also need directional transformation

On the other hand, the silkworm species used in sericulture production at home and abroad are preferably the first-generation hybrids of the dimorphic Chinese system and the Japanese system, using the Chinese patent application "Posterior silk glands can synthesize and secrete hydrophilic sericin The preparation method of the silkworm from "(Application No.: 201610348866.0) The Japanese systemic variety transgenic mutant silkworm lacks the high combining ability of the Chinese systematic inbreeding variety, and cannot efficiently produce a generation of hybrids for silkworm production

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