SNP (Single Nucleotide Polymorphism) molecular marker closely linked with eggplant fruit color and application
A technology of molecular markers and eggplant fruits, applied in the field of molecular genetic breeding, can solve the problems of large workload, time-consuming and laborious, long breeding cycle, etc., and achieve the effect of reducing the workload of breeding and shortening the time of gene positioning
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[0035] Example: SNP molecular markers closely linked to eggplant fruit color , Its positioning and application steps are as follows:
[0036] 1. Construction and genetic analysis of EMS mutants
[0037] 1. Test plant material
[0038] Using EMS to mutagenize the purple-red eggplant inbred line, a stable genetic white eggplant mutant was obtained. The pericarp of the mutant was white and the total anthocyanin content was significantly reduced, such as figure 1 shown. The mutant is continuously selfed for multiple generations to obtain a homozygous inbred line, and after backcrossing the white eggplant mutant and the purple eggplant wild type, the F1 generation is generated, such as figure 2 As shown, the phenotypes were normal, all of which were red eggplants, showing recessive inheritance. figure 1Photographs of wild type (WT), mutant eggplant (L6-5) and F1 generation eggplant. F1 generation eggplants were selfed to obtain F2 generation eggplants, and F2 generation eggpl...
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