28 results about "Sucrose solution" patented technology

The invention provides Saccharomyces cerevisiae for producing squalene as well as a screening method and application of the Saccharomyces cerevisiae. The method comprises the following steps: culturing a Saccharomyces cerevisiae engineering strain ySC782 for synthesizing squalene to obtain a to-be-mutagenized bacterial solution, carrying out ARTP mutagenesis on the to-be-mutagenized bacterial solution, adding the to-be-mutagenized bacterial solution into a YPD liquid culture medium, culturing to obtain a resuscitation bacterial solution, adding the resuscitation bacterial solution into a sucrose solution with gradient concentration distribution, screening, and repeating the ARTP mutagenesis-sucrose solution screening step to obtain the squalene mutant strain. And finally, the saccharomyces cerevisiae strain ySC782-M5 is obtained through screening, and the saccharomyces cerevisiae strain ySC782-M5 is obtained through screening. The yield of squalene produced by the saccharomyces cerevisiae ySC782-M5 reaches 49.83 g / L, so that a solution is provided for breaking through the yield bottleneck of squalene synthesized by yeast.

The invention discloses a composition containing ginseng exosome and super-elastic protein and application of the composition, and belongs to the technical field of biology. The composition containing the ginseng exosome and the super-elastic protein is prepared by compounding the ginseng exosome and the super-elastic protein according to the weight ratio of 10: (100-150), the ginseng exosome is prepared by the following method: extracting the ginseng exosome from ginseng root tissues by using a differential centrifugation method to obtain a ginseng exosome crude extract; adding sucrose solutions with different concentrations into the ginseng exosome crude extract, and performing ultra-high-speed centrifugal purification to obtain purified ginseng exosome; the amino acid sequence of the super-elastic protein is as shown in SEQ ID NO. 3. According to the invention, the ginseng exosome and the super-elastic protein are combined for use for the first time, and both the ginseng exosome and the super-elastic protein have the effect of promoting cell proliferation, so that the use safety is high; the ginseng exosome and the super-elastic protein can synergistically improve the skin tightening and anti-wrinkle effects and the skin injury repairing effect.

The invention relates to a germplasm cryopreservation method suitable for bait microalgae, and belongs to the field of algae preservation, and the method comprises the following steps: carrying out low-temperature domestication on microalgae in a logarithmic phase, and centrifugally collecting algae mud; mixing the algae mud with a protective solution for pretreatment; after pretreatment, mixing with a cryoprotectant with specific composition, carrying out gradient cooling, and storing at-80 DEG C; during thawing, the cryopreservation tube is quickly thawed in a water bath, and the cryoprotectant is subjected to gradient elution through a sucrose solution. The method successfully realizes long-term stable preservation of various bait microalgae at-80 DEG C by optimizing a cryoprotectant combination and optimizing key parameters such as a protective solution, balance time and thawing temperature, has high survival rate and stable physiological traits after resuscitation, and is low in cost, convenient to operate and suitable for industrial production. And a new technical scheme is provided for protection and utilization of microalgae germplasm resources.

The utility model relates to the technical field of filtering for sucralose decoloration, in particular to a filtering device for sucralose production, which comprises a coarse filtering pipe and a fine filtering pipe which are vertically arranged, the coarse filtering pipe is communicated with the fine filtering pipe through a transverse pipe, and the fine filtering pipe is detachably connected with the transverse pipe. The lower end of the coarse filter pipe is closed, the upper portion of the coarse filter pipe is communicated with a filter screen, the lower end of the fine filter pipe is closed through a baffle, a plurality of water outlet holes are formed in the baffle, filter cloth is laid on the top face of the baffle, and a pressing plate matched with the inner diameter of the suction filter pipe and capable of being arranged above the transverse pipe is arranged above the fine filter pipe. The pressing plate is driven by a driving device to move up and down along the suction filter pipe. By using the sucralose filtering device, the sucralose solution can be secondarily filtered by using the filter screen and the filter cloth, so that the sucralose solution can be prevented from containing carbon powder, and the purity of the sucralose solution is ensured.

The application provides a method for extracting and application of broccoli exosome, and the extraction method is as follows: (1) cut the washed broccoli into pieces, mix with PBS buffer, beat pulp, filter, and take the supernatant; (2) centrifuge the supernatant obtained in step 1 for multiple times; (3) ultracentrifuge the supernatant obtained in step 2 after multiple centrifugations, reserve the precipitate, and resuspend with PBS buffer; (4) ultracentrifuge the suspension in step 3 in sucrose solutions with different concentrations, and collect and combine the bands between different concentration sucrose solution layers; (5) mix the bands obtained in step 4 with PBS buffer, ultracentrifuge, remove sucrose, and the obtained precipitate is broccoli exosome. Meanwhile, the application also provides application of the broccoli exosome obtained by the above extraction method in preparation of a medicine for relieving constipation.

This invention relates to the field of molecular biology, and more specifically, to a novel application of *Tea glomerata* exovesicles, namely, their application in the preparation of anti-inflammatory drugs, specifically in drugs for treating acute pneumonia. The method for preparing *Tea glomerata* exovesicles includes the following steps: Fresh *Tea glomerata* slices are juiced, and the juice is collected. The juice is centrifuged sequentially, and the supernatant from the third centrifugation is collected and centrifuged at 120,000-140,000g for 50-60 minutes to obtain a crude extract. The crude extract is added to a gradient concentration sucrose solution, centrifuged, and a solution containing 30%-45% bands is collected. This solution is then centrifuged at 150,000-160,000g for 50-60 minutes to obtain the *Tea glomerata* exovesicles. The method of this invention can yield *Tea glomerata* exovesicles that meet standards and have stable quality.

The present application relates to a kind of complex component radioactive waste liquid processing method, steps are as follows: organic matter is contained in waste liquid and sodium hydroxide and sucrose solution is added to pretreat and carry out oxidation decomposition treatment;pH value is adjusted, steam heating is carried out, ammonia in waste liquid is recycled under the condition of high gravity state and is rotated and separated, and after the pH value of deamination waste liquid is adjusted, it is input into first reverse osmosis unit, and is concentrated in circulation, and the concentrated first concentrated phase is carried out rotary evaporation and crystallization, and waste liquid steam and first dilute phase are sent to second reverse osmosis unit and are concentrated in circulation, and the obtained second concentrated phase is recycled;Second dilute phase is discharged or it is input into reverse osmosis+EDI unit and is deeply treated and reused.The present application designs the process flow of preferentially treating waste liquid containing organic matter, and then treating waste liquid not containing organic matter, forms simple and efficient process route;With the advantages of efficient and clear, purification is thorough, easy to operate, simple and reliable, effectively solve the purification treatment problem of complex component radioactive waste liquid.

The invention relates to the technical field of extraction of fresh idesia polycarpa fruits, in particular to a preparation method of high-stability phenol-rich oil from fresh idesia polycarpa flesh. The method comprises the following steps: adjusting the pH value of pulp of fresh idesia polycarpa fruits to 4-7, then adding a first enzyme seed and a second enzyme seed, extracting for 0.5-4 hours at the temperature of 30-50 DEG C, centrifuging, and collecting a first emulsion; the first enzyme comprises pectinase, and further comprises at least one of hemicellulase and cellulase; the second enzyme is composed of tannase and glycosidase; and washing the first emulsion with water or a sucrose aqueous solution, centrifuging, and collecting a second emulsion to obtain the oil body. Through simultaneous enzymolysis of the first enzyme and the second enzyme, free polyphenol is generated in situ around the oil body, interaction of the oil body and polyphenol is promoted, part of polyphenol, protein and pectin are combined on the surface of the oil body, part of weak-polarity polyphenol enters the oil body, and the physical and chemical stability of the oil body is synergistically improved.

The present invention discloses a calcium hydroxide content detection apparatus, and relates to the technical field of calcium hydroxide content detection, the calcium hydroxide content detection apparatus comprises an identification assembly for adding water and a hydrochloric acid standard titration solution into a sample, the identification assembly comprises a camera, and the camera performs photographing analysis on the color change of the sample, by comparing an HSV color space, when the sample becomes colorless is judged, meanwhile, the usage amount of a hydrochloric acid standard titration solution is determined, the content of calcium hydroxide in the sample is calculated, and the sample is placed in a stable light environment by the recognition assembly in the analysis process, so that the accuracy of photographing analysis of the sample is improved; the device is also provided with a transfer assembly and a stirring assembly, the transfer assembly drives the sample to move to a working position and shakes up the transfer assembly, and the stirring assembly stirs the sample added with the sucrose solution, so that the detection efficiency and accuracy are improved.

The invention discloses a non-steroidal anti-inflammatory drug suspension drop and a preparation method thereof. The suspension drop comprises ibuprofen, xanthan gum, pregelatinized starch, glycerin, sucrose, anhydrous citric acid, sodium benzoate, polysorbate 80, strawberry essence and water. The preparation method comprises the following steps: 1) sieving ibuprofen with a 20-mesh sieve for later use; the preparation method comprises the following steps: adding polysorbate 80 into purified water, stirring and dissolving, adding ibuprofen according to the prescription amount, and stirring and dispersing uniformly; 2) adding 100 DEG C purified water and glycerol into the liquid preparation tank, and stirring and mixing; (3) starting a rotor pump and a homogenizing pump to form internal circulation in the liquid preparation tank, opening a ball valve of a charging hopper, adding xanthan gum and pregelatinized starch, closing the valve of the hopper, and homogenizing and stirring until the step (6) is finished; (4) weighing cane sugar, adding the cane sugar into boiling water at 100 DEG C, and stirring for dissolving; 5) continuously stirring in the liquid preparation tank, adding a sucrose solution, washing the container with water, and adding into the liquid preparation tank; 6) adding anhydrous citric acid and sodium benzoate into the liquid preparation tank, and stirring for more than 1 hour after the steps 3-6 are finished; 7) closing the homogenizing pump, adding an ibuprofen dispersion liquid into the liquid preparation tank, washing the dispersion container with water for several times, and stirring; (8) adding essence into the liquid preparation tank, and stirring; 9) adding the total amount of purified water, and stirring; and 10) filling. The drops with good dispersibility and good stability are obtained by screening the prescription and optimizing the preparation process.

The invention discloses beta-cyclodextrin as well as a preparation method and application thereof by taking sucrose as a substrate, and belongs to the technical field of cyclodextrin production. The method comprises the following steps: by taking sucrose as a raw material, firstly adding a sodium citrate buffer solution to prepare a sucrose solution, regulating the temperature and the pH value of a feed liquid, adding a sucrose glucosyltransferase mutant and a cyclodextrin glucosyltransferase mutant to react, transferring and heating the feed liquid after the reaction is finished, adding licolal high-temperature-resistant amylase, and continuously stirring to obtain the sucrose-cyclodextrin glucosyltransferase mutant. The preparation method comprises the following steps: preparing beta-cyclodextrin, then decolorizing and filtering the feed liquid through activated carbon, adding n-hexane into the filtrate, crystallizing at room temperature, finally removing n-hexane through evaporation, removing oligosaccharide through filtration, and performing vacuum drying to obtain the beta-cyclodextrin. The invention discloses a preparation method for producing beta-cyclodextrin by adopting multienzyme reaction, the prepared beta-cyclodextrin has good purity and recovery rate, metal ions do not need to be added for assistance, the preparation process is simple, convenient and easy to control, and efficient preparation of the beta-cyclodextrin can be realized.

The invention discloses a flexible sucrose coating, a preparation method thereof and a sensor. The preparation method of the flexible sucrose coating comprises the following steps: carrying out first heat treatment on a sucrose solution containing a flexible substance to obtain an amorphous sucrose film; carrying out friction treatment on the amorphous sucrose film to generate seed crystals on the surface layer, and then carrying out second heat treatment to obtain a first coating; the step of preparing the first coating is repeated, a plurality of coatings continue to be formed on the surface of the first coating, and the flexible sucrose coating is obtained. According to the method, large-scale film preparation equipment is not needed, the flexible sucrose coating with good detection performance can be constructed by repeatedly preparing the multiple sucrose coatings, growth of sucrose crystals in a proper direction is activated through friction treatment, the direction is a b axis perpendicular to the surface, and the strongest longitudinal wave can be generated in the direction; the thickness of the flexible sucrose coating can be well adjusted, and the flexible sucrose coating can be formed on the surface of a sample in any shape.

The invention relates to a frozen microneedle loaded with hair follicle stem cells as well as a preparation method and application of the frozen microneedle, and relates to the technical field of frozen microneedles. The freezing microneedle comprises a substrate and a plurality of needle bodies distributed on the substrate in an array mode, the substrate is prepared from a substrate solution, the needle bodies are prepared from hair follicle stem cells and an anti-freezing mixed solution, the substrate solution is a sucrose-PBS solution, and the anti-freezing mixed solution comprises sodium alginate, polyvinyl alcohol, dimethyl sulfoxide and a sucrose-PBS solution. According to the anti-freezing mixed solution prepared by the preparation method provided by the invention, the transdermal mechanical strength of the microneedle can be improved while the living cell loading of the frozen microneedle is ensured, and the frozen microneedle loaded with the hair follicle stem cells prepared by the scheme can realize a minimally invasive cell therapy for promoting hair growth.

The application provides a preparation method and a kit of Nymphoides peltatum leaf protoplast. The application takes Nymphoides peltatum leaf as material, adjusts an enzymolysis system, a washing system and a purification method, and establishes a Nymphoides peltatum leaf protoplast preparation system. The enzymolysis effect is good when 1% cellulase, 0.5% pectinase and 0.5% macerase are in the enzymolysis liquid system; the compounding of MES, sorbitol and KCl can ensure that the external medium and the osmotic potential of the cell itself are the same when the protoplast is separated, maintain the activity of the protoplast, and the 26% sucrose solution can better maintain the osmotic pressure stability of the protoplast; the method can quickly and efficiently dissociate the protoplast cells of Nymphoides peltatum leaf tissue, the protoplasts obtained by separation are numerous, have high activity and a clean background. The result can be directly used for single cell sequencing, and provides an important basis for exploring the molecular cell biology research and the molecular mechanism of development of Nymphoides peltatum heterophyllous leaves.

The utility model relates to a device for continuously preparing cane sugar seed crystals in batches, which comprises a cane sugar solution storage tank, an ethanol storage tank, an opposed nozzle mixing tank and a seed crystal collection storage box, and the opposed nozzle mixing tank comprises a mixing tank body and 1-2 groups of nozzles. Each group of nozzles consists of a cane juice nozzle and an ethanol nozzle which are oppositely arranged on the mixing tank body, the central axes of the cane juice nozzle and the ethanol nozzle in the same group of nozzles coincide, and the cane sugar solution storage tank is communicated with the cane juice nozzle of the mixing tank with the opposite nozzles through a pipeline; and the ethanol storage tank is communicated with the ethanol nozzle of the opposed nozzle mixing tank through a pipeline. The device is simple in structure, can be used for continuously preparing sucrose seed crystals on a large scale, and is high in production efficiency, and the prepared seed crystals are regular in shape, uniform in particle size distribution and good in quality.

The invention discloses a method for establishing a bee Parkinson's disease model based on characterization of dyskinesia and the like, which is characterized by comprising the following steps of: animal selection: selecting a mature bee as an object for establishing the bee Parkinson's disease model; preparing a modeling diet: preparing a sucrose solution containing casein hydrolysate and rotenone; and model establishment: feeding the mature bees with a modeling diet for 7 days to establish the bee Parkinson's disease model. The invention further discloses an application of the parkinson's disease model, established by the method, of the bacteria bees and / or the sterile bees. The method is easy and convenient to operate and low in cost, the motion distance and the motion speed of the built model bee are remarkably decreased, obvious dyskinesia is shown, and the survival rate of 5 days in an existing bee Parkinson model building technology is effectively increased to about 80% of 7 days from 5 days lower than 30%. The bee Parkinson's disease model established by the method can be used for screening new methods or new drugs for preventing or treating dyskinesia related diseases.

The invention relates to the technical field of biology, in particular to a method for preparing fructo-oligosaccharide through an enzyme method. The fructo-oligosaccharide with high kestose content is obtained by utilizing the compound stabilizer to improve the enzyme activity, supplementing the sucrose solution in batches, realizing double-enzyme synergism, improving the substrate utilization rate, improving the rejection rate by utilizing the compound nanofiltration membrane and realizing a gradient temperature control synergistic effect.

A gluten-free baked good is provided. The gluten-free cookie, cracker, or wafer includes one or more gluten-free flours, 3-10 wt% pregelatinized starch, 3-15 wt% fat, and 1-32 wt% sweetener; wherein the gluten-free flour has a D90 of less than about 125 µm and includes rice flour, oat flour, buckwheat flour, sorghum flour, potato raw starch, or combinations thereof; wherein the pregelatinized starch is pregelatinized corn starch; and wherein the combination of the gluten-free flour and the pregelatinized starch has a solvent retention value greater than 100 for a 50% sucrose solution, a solvent retention value less than 75 for a 5% sodium carbonate solution, and a solvent retention value less than 75 for water. These cookies have a taste, texture, and other properties similar to baked goods made with wheat flour despite the absence or low levels of gluten.

The invention discloses a method for extracting bee mitochondria. The method comprises the following steps: (1) adding a bee tissue specific buffer solution into bee tissues, and homogenizing to obtain homogenate; (2) adding trypsin into the homogenate, oscillating, and standing on ice to obtain lysate; (3) centrifuging the lysate by using a differential centrifugation method, and resuspending and precipitating by using a bee tissue specific buffer solution to obtain a crude extract; and (4) taking 15-35% (w / v) sucrose solution as a gradient medium, centrifuging the crude extract by a density gradient method, collecting suspended solids in 25-35% (w / v) sucrose solution, and centrifuging to obtain the bee mitochondria. The bee mitochondria extraction method is based on a bee tissue specific buffer solution with an optimized formula, and compared with a commercially available kit, the activity and concentration of the extracted mitochondria can be effectively improved; and the mitochondria obtained based on the extraction method is high in integrity, and a foundation is laid for subsequent downstream experiment research related to the bee mitochondria.

This application relates to a method for deodorizing tilapia using yeast fermentation and its application, belonging to the field of aquatic product processing and flavor control technology. This application aims to solve the technical problems of complex deodorization processes, unstable flavor improvement, and the tendency to produce off-odors in existing tilapia technologies. The tilapia deodorization method using yeast fermentation of this application includes the following steps: S1, adding highly active dry yeast to a sucrose solution to prepare a yeast culture; S2, heating the yeast culture and adding it to tilapia meat, adjusting the pH of the system, fermenting, and filtering after fermentation to obtain deodorized tilapia meat. The method provided in this application utilizes the metabolic activity of yeast to reduce the content of fishy or irritating volatile substances such as 2,3-octanedione, 2,4-decadienal, and 2-nonenal in tilapia, and increases the content of sweet-smelling components such as ethanol, phenethyl alcohol, 2-acetylpyrrole, and esters, thereby achieving deodorization and flavor enhancement.

This invention relates to the field of microwave absorbing material preparation technology, and discloses a method for preparing composite microwave absorbing materials based on decolorized waste attapulgite. Specifically, the method involves mixing decolorized waste attapulgite and red mud, grinding them evenly, then adding waste cooking oil and stirring evenly. Next, an equal amount of sucrose solution is added to the solid mixture, stirred evenly, dried, ground, and placed in a tube furnace. Nitrogen gas is introduced, and the temperature is increased to 700-800℃ at a rate of 5℃ / min, and held for 2-3 hours to prepare the composite microwave absorbing material. This invention produces a composite microwave absorbing material with excellent microwave absorption performance, realizing high-value-added resource utilization of various types of waste.

The present application relates to the technical field of molecular biology, more particularly to the application of camellia sinensis var. assamica exovesicles in the preparation of anti-inflammatory drugs, and a preparation method of the camellia sinensis var. assamica exovesicles, comprising the following steps: collecting juice after fresh camellia sinensis var. assamica slices are juiced, sequentially centrifuging, and collecting the supernatant of the third time, centrifuging at 120000g-140000g for 50-60min to obtain a crude extract; adding the crude extract into a gradient concentration sucrose solution, centrifuging, collecting the solution of the band between 30%-45%, and then centrifuging at 150000g-160000g for 50-60min to obtain the camellia sinensis var. assamica exovesicles. The camellia sinensis var. assamica exovesicles prepared by the method of the present application meet the standards and are stable in quality. The present application also obtains miRNAs related to anti-inflammatory treatment in the camellia sinensis var. assamica exovesicles.

The invention discloses a method for increasing the content of fructo-oligosaccharide and co-producing calcium gluconate, and belongs to the technical field of biological engineering. The invention discloses a method for increasing the content of fructo-oligosaccharide and co-producing calcium gluconate, which comprises the following steps: adding fructosyl transferase, glucose oxidase and catalase into a sucrose solution and regulating the pH value to obtain a reaction solution of fructo-oligosaccharide and calcium gluconate; and filtering, crystallizing, centrifuging and drying the reaction liquid to obtain calcium gluconate. And meanwhile, carrying out ion exchange on the centrifugate, and concentrating to obtain the fructo-oligosaccharide syrup. According to the method disclosed by the invention, the high-content fructooligosaccharide is directly prepared from the sucrose through the synergistic effect of the fructosyl transferase, the glucose oxidase and the catalase, the purification and impurity removal processes in the fructooligosaccharide production process are simplified, and efficient separation of the fructooligosaccharide and the calcium gluconate is realized through subsequent processes of filtration, crystallization and the like; the co-production of fructo-oligosaccharide and calcium gluconate is realized, so that the resource utilization efficiency is greatly improved.

This invention discloses a method for producing low-GI brown sugar from raw sugar, belonging to the field of edible sugar production technology. Using food-grade cane raw sugar as the core ingredient, the method involves first preparing a homogenized sucrose solution by constant-temperature re-dissolving of the raw sugar. This solution is then gently purified using physical low-pressure filtration combined with natural plant clarifying agents, without removing the natural honey aroma substances, minerals, and natural pigments of the sugarcane. Subsequently, natural slow-glycemic prebiotic components are added to the purified sugar solution, and a low-temperature gradient cooking process is used to control the slight polymerization degree of sucrose, inhibiting rapid decomposition and absorption. The glycemic rate is optimized solely through physical modification and natural compounding, without the use of large-scale biological enzyme conversion of sucrose. After cooking to the required standard, the product is subjected to low-temperature slow coagulation, natural shaping, and low-temperature drying to obtain the finished low-GI brown sugar.

The present application relates to a method and system for detecting the sweetness perception threshold based on dynamic gradient feedback, and belongs to the technical field of animal behavior detection. The method comprises: configuring sucrose solutions with gradient concentrations; collecting the time and pressure of the animal contacting the bottle mouth respectively; calculating the contact proportion of the animal to the sucrose solution under each concentration; constructing a mathematical model to fit the relationship curve between the contact proportion and the concentration of the sucrose solution; determining whether there is a significant change in the animal's perception of the sugar concentration according to the slope, and determining the sweetness perception threshold; and inducing sugar solution aversion reaction to verify whether the threshold result is reliable. The present application introduces the L w slope of the value-concentration curve to analyze the sensitivity of the animal to sucrose solutions with different concentrations, which can effectively distinguish between physiological perception and random preference, and effectively improve the accuracy of sweetness perception threshold detection; and the calculation of L w integrates the double parameter indicators of contact time and licking pressure, further improving the accuracy of sweetness perception threshold detection.

The application belongs to the technical field of polymer materials, and particularly discloses a preparation method of modified polyester master granules, a preparation method of black polyester film, and the black polyester film prepared by the method. The preparation method comprises the following steps: dissolving a sucrose solution in desalted water; uniformly dispersing silicon dioxide powder into the sucrose soaking solution and soaking for a predetermined time; putting the silicon dioxide sucrose soaking solution after soaking for the predetermined time into an evaporation container to completely evaporate, so as to obtain sucrose-silicon dioxide; placing the sucrose-silicon dioxide into a heating furnace, heating to a set temperature, and keeping warm for a set time, so that the sucrose is completely carbonized to obtain modified silicon dioxide; and then preparing modified PET polyester master granules. The obtained modified silicon dioxide can replace part of carbon black materials for preparing a film surface layer, and when producing full-shading black polyester film, the addition amount of core layer carbon black powder can be greatly reduced, thereby reducing the production cost.

The present application relates to the technical field of plant tissue protoplast separation, and in particular to a preparation method and application of Phyllostachys edulis root tip protoplast. The preparation method comprises the following steps: collecting root tips of Phyllostachys edulis spring bamboo shoots as raw materials; washing the raw materials with 0.6-0.8M mannitol, and then placing the washed raw materials in an enzymatic solution for enzymolysis for 2.5-3.5h to obtain a protoplast single cell suspension; centrifuging the protoplast single cell suspension at 40g-60g, removing the supernatant, resuspending the cells with mannitol, and obtaining a cell resuspension; mixing the cell resuspension with a sucrose solution with a mass concentration of 18-22%, and then centrifuging at 40g-60g to obtain the protoplast. The preparation method can obtain protoplast with high cell activity and pure background, and can meet the use requirements of Phyllostachys edulis root single cell transcriptome sequencing library construction, and has great significance for Phyllostachys edulis root single cell sequencing.

The application discloses an inosine oral solution and a production process thereof, relates to the technical field of oral solution production, and comprises the following steps: S1: dissolving sucrose in purified water to obtain a sucrose solution; S2: taking inosine, sodium benzoate and stevioside, dissolving the inosine, the sodium benzoate and the stevioside in water to obtain a mixed solution, then adding composite adsorption microspheres into the mixed solution, filtering, uniformly mixing the filtrate with the sucrose solution, continuously adding purified water, and simultaneously adjusting the pH value by using sodium hydroxide; filtering and filling; wherein, the preparation method of the composite adsorption microspheres is as follows: mixing activated carbon, vermiculite and magnesium lithium silicate, uniformly grinding the mixture to obtain pretreated raw materials, adding the pretreated raw materials into silica sol for granulation, then performing electrode treatment to obtain the composite adsorption microspheres. The application can effectively further remove impurities in inosine by adopting the specific composite adsorption microspheres to adsorb the mixed solution, so that the influence of the impurities on the stability of the inosine is reduced.