468 results about "Sucrose solution" patented technology

A plurality of chromatographic separation operations, including a first simulated moving bed operation, are coupled into a process which functions, preferably through the application of continuous displacement chromatography, to recover a fraction rich in small organic molecules, notably betaine and / or invert from sucrose solutions, enabling the subsequent production of a high purity sucrose product.

A plurality of chromatographic separation operations, including a first simulated moving bed operation, are coupled into a process which functions, preferably through the application of continuous displacement chromatography, to recover a fraction rich in small organic molecules, notably betaine and / or invert from sucrose solutions, enabling the subsequent production of a high purity sucrose product.

This invention discloses a method for preparing multi-level porous beta-zeolite. The method comprises: soaking monolithic silica gel column in sucrose solution, drying, polymerizing, carbonizing to obtain C-Si composite, wetting the C-Si composite with a mixed solution of Al source, inorganic alkali, organic amine and water, crystallizing, and recovering the product. In this method, carbon material can effectively protect the porous structure of monolithic silica gel column from damage during crystallization process. The obtained beta-zeolite has multi-level pores, including micrometer-scale macropores, mesopores and micropores. The macropores can shorten the diffusion distances of reactive molecules, reduce the pressure decrease of the equipment, raise the unit processing capacity of the equipment, and make the adjustment and control of the product selectivity easier. The mesopores can provide large inner specific surface area, which is meaningful to the catalytic reaction of macromolecules.

An article to be inserted into the human body has a superhydrophobic surface. The superhydrophobic surface is coated with a water soluble thin but durable protective coat. One positioned inside the body the coating is rapidly dissolved by the blood or other fluids and exposes the superhydrophobic surface. To coat article the water based coating is mixed with a liquid capable of wetting the superhydrophobic surface but is still dissolvable or at least miscible in the coating. As an example, a glucose or sucrose solution in water is mixed with alcohol and used to coat the surface. After water and alcohol evaporation, a durable protective coat of sugar remains. After the coated article is inserted into the body, the coating is rapidly dissolved and absorbed by the body.

A method for intensifying limonite sintering has the advantage of changing the property of the moisture of the mixture by adding sucrose solution with weight concentration being 4-10%. The solution has certain viscosity which is reduced with the increase of the temperature. In the processes of mixing and granulating, the mineral powder with fine grains is well adhered to the surfaces of pellets, thus intensifying limonite granulating, improving the intensity of the pellets of the mixture and achieving the aims of intensifying limonite sintering, improving the quality of sintered minerals and reducing solid fuel consumption. The method has better effects especially on the limonite with finer grain sizes. The sucrose has the greatest characteristic of totally reacting in the process of sintering and leaving no residues in the sinter, thus having no impact on the quality of the sinter.

The invention discloses a method for determining influence of exogenous dsRNA on toxicity of ladybugs. The method comprises the following specific steps: uniformly mixing the exogenous dsRNA into a sucrose solution, directly feeding the ladybugs for 2-3 days and then feeding with pea aphids; then detecting and analyzing the expression changes of target genes in the ladybugs, and observing the biological changes of the ladybugs so as to evaluate the toxicity of the exogenous dsRNA to the ladybugs, wherein the ladybugs include harmonia axyridis, coccinella septempunctata or henosepilachna pusillanima. By the method, direct influence of the exogenous dsRNA on the toxicity of the harmonia axyridis, the coccinella septempunctata or the henosepilachna pusillanima or other ladybugs can be effectively determined; in addition, the method is simple, feasible and high in effectiveness and sensitivity, and has a great significance and an application prospect in study on functions of related genesas well as environmental risk assessment on related RNAi transgenic crops.

The invention discloses a method for preparing potato crispy chips through osmotic dehydration and hot air and microwave combined drying, and belongs to the technical field of fruit and vegetable processing. The method mainly comprises the following steps of: cleaning, peeling and slicing potatoes (the thickness of the slices is 4-8mm), blanching for deactivating enzyme (at the temperature of between 90 and 110DEG C), performing osmotic dehydration (penetrating fluid, namely a 40 mass percent sucrose solution is prepared, a mass ratio of the potato crispy chips to the penetrating fluid is 1:(10-12), the soaking temperature is 30-40DEG C, and the soaking time is 30 to 40 minutes), draining water, drying with hot air at the temperature of between 60 and 70DEG C at the air speed of 1-3m / s to obtain a semi-finished product of which the water content is 30 to 40 percent, and performing microwave drying (1-3W / g) finally to obtain the finished product of which the water content is 2 to 5 percent. A new process of assisting in dehydrating through osmotic soaking and performing normal-pressure hot air and vacuum microwave combined drying is adopted, the obtained potato product has nutrient components and color which are maintained well, has crispy texture and avoids defects of high oil content and greasy mouthfeel of a fried food, the production cost is far lower than that of a vacuum freeze-drying product, and the method has the characteristics of short production period, low cost, and good product quality.

The invention publishes the preparation methods to produce ass hide and ginseng and stibene wine. This medicated wine has function of curing and health protection. The distilled spirit acts as groundsustance for ass hide and ginseng and stibene wine and then dip ginseng, prepared rehmannia root, dangshen, medlar, astragalus root, angelica, poria conos, licorice root, chuanxiong rhizome, white fat peony and bighead atractylodes rhizome, after this, add ass hide and sugar liquor to get the medicated wine. The thirteen good drug are mixed reasonably, the distilled spirit is used to dip and distill and sugar is used to season, so the drug can be absorbed by men. The invention has many function, such as helping buiding blood and invigoratin qi, strengthening the spleen and stomach, nourishing the heart and tranquilization, nourishing yin and moisturizing the lung, promoting blood circulation and improving acuity of vision and refreshment. It is good for not only weaken habitus but also blood vessel of brain and heart and gynecology illness.

The invention relates to a preparation method for an iron sucrose bulk drug. The method comprises the following steps: (1) crystallization: the pH value of sucrose solution is adjusted to 1-4 with acetic acid, iron salt solution is added to the sucrose solution to form mixed liquor, the pH value of the mixed liquor is adjusted to 1-3 with Na2CO3 solution, then the mixed liquor is stirred and is continued to be added with the Na2CO3 solution until turbidity appears in the mixed liquor, the pH value of the mixed liquor is adjusted to 4-7, and then the mixed liquor is filtered and added with water to obtain iron cake solution; (2) complexing: the sucrose solution is heated and added with alkaline liquor for alkalization, and then the alkalized sucrose solution is added with the iron cake solution and stands for the night to obtain complexing solution; and (3) refining: the complexing solution after standing for the night is filtered, and the filtrate precipitates with 2-3 times of 95 percent of ethanol and is filtered to obtain the iron sucrose bulk drug. The invention has the advantages of simple process steps, easy control of the reaction processes, good product quality, high yieldand lower production cost and is a high-novelty preparation method for the iron sucrose bulk drug and the injection thereof.

The invention provides a nano-gold catalyst supported on combined metal oxide, a preparation method and an application thereof. The preparation method comprises the following steps: preparing layered double hydroxides (LDH) precursor crystal nucleus and dispersing the LDH precursor crystal nucleus into sucrose solution; then adding chloroauric acid solution in the obtained mixture so as to reduce Au3+ in HAuCl4 to Au single substances by using the reduction of glucose and fructose produced by sucrose hydrolysis; in the process of reduction, crystallizing the LDHs so as to obtain solid LDH supported on nano-gold; and carrying out high-temperature roasting on the solid LDH supported on the nano-gold so as to obtain the nano-gold catalyst supported on the combined metal oxide. The combined oxide of the catalyst is taken as a carrier on which the nanosize gold particles is supported, wherein the combined oxide refers to MgO or compound of ZnO and Al2O3, and the mole ratio of the MgO to the compound of ZnO and Al2O3 is 2-6:1; the gold capacity is 0.5 to 3 percent, the size of the gold particle is 8 to 15nm, and the gold particle is ellipsoidal or polyhedral. When the catalyst is used in the catalytic hydrogenation reaction of unsaturated aldehydes, the conversion rate of reactants reaches 60 to 97 percent, and the selectivity of cinnamyl alcohol is 40 to 75 percent.

The invention discloses a method for portably and rapidly detecting ochratoxin A. The method comprises the following steps: first, preparing sucrose invertase-terminal alkyne modified DNA; subsequently, combining biotin-nitrine modified DNA with the surface of a magnetic bead, adding the ochratoxin A with different concentration, and mixing; adding the previously-prepared sucrose invertase-terminal alkyne modified DNA, a CuSO4 solution and an ascorbic acid solution, wherein the ascorbic acid solution can be used for reducing Cu(II) to obtain a Cu(I) compound so as to catalyze the biotin-nitrine modified DNA and the sucrose invertase-terminal alkyne modified DNA to have a click chemistry reaction; taking a supernatant liquor after the reaction is ended, then adding the supernatant liquor into a sucrose solution, wherein the sucrose invertase can effectively catalyze the sucrose in the solution to be transformed into glucose; and finally, measuring by adopting a glucometer. The method is used for organically combining the rapid reaction advantage of the click chemistry reaction with the high specificity characteristic of an aptamer, and adopting the simple and portable glucometer to acquire data, thus the operation complexity and detection cost are greatly reduced, and the sensitivity and selectivity are improved.

The invention discloses a method for preparing high fructose corn syrup, comprising the following steps: (1) adding sucrose hydrolase to a sucrose solution for hydrolyzation and carrying out filtration, discoloration, ion-exchange, concentration and chromatographic resolution on the enzymatic hydrolysate to respectively gain high-purity glucose liquid and fructose liquid; (2) carrying out discoloration, ion-exchange, nanofiltration membrane treatment and concentration on the resulting fructose solution to prepare high fructose corn syrup F90; (3) carrying out concentration, isomerization, discoloration, ion-exchange and concentration on the resulting glucose liquid to prepare high fructose corn syrup F42. By adopting the method, sucrose is decomposed by saccharase, fructose purity is increased by resin decolorization and chromatographic resolution, and impurities such as oligosaccharide are removed by a nanofiltration membrane, thus effectively improving the quality of high fructose corn syrup and simultaneously producing sugar liquor with three purities at most to meet different demands.

The invention relates to a fermenting method for producing pullulanuse polysaccharide, which is characterized in that the method comprises following steps: firstly, preparing seed culture medium, secondly, preparing fermenting initial culture medium, thirdly, inoculating liquid seeds into the fermenting initial culture medium, wherein the inoculation amount is 3-8%, the fermentation stirring speed is 200-500rpm, the fermentation temperature is 29 DEG C +- 1 DEG C, the ventilation volume is 0.5-10V / V, and the bed pressure is 0.01-0.02MPa, fourthly, complementarily adding the other carbon source after fermenting for 24 hours, wherein the adding amount is 40-80g / L and the adding liuqid is sucrose solution or glucose solution or starch hydrolysate whose pH value is 40-60, and continuingly fermenting for 60-72 hours after adding the carbon source, fifthly, flocculating, hyper-filtrating, barrier-separating, concentrating and drying the fermenting liquid and obtaining the pullulanuse polysaccharide whose molecular weight is between 20-60 million daltons. The invention has simple technique and significant effects and prominently shortens the fermenting time, the fermenting time is 80-100 hours, the production efficiency is increased greatly, energy is saved, the yield rate of product is greatly improved, and the yield can reach by 50%.

The invention relates to a process for preparing syrup through sucrose conversion, which consists of steps including dissolving sucrose with hot water, obtaining sucrose solution, charging edible sour flavor materials as conversion agents for sucrose hydrolytic reaction, adjusting pH of the hydrolysis products to 2. 8-3. 8, filtering, vacuum concentrating and cooling down. the obtained sugar syrup product has a concentration of 70-80 Deg Bx, and a conversion degree of 5. 0-99. 5%.

The invention discloses an aptamer of aflatoxin B1 and a complementary DNA sequence of the aptamer, further discloses a method for quantitatively detecting aflatoxin B1, and belongs to the field of quantitative detection of aflatoxin B1. The method includes the following steps that 1, an aptamer biosensor is prepared; 2, aflatoxin B1 in a sample to be detected is extracted to obtain sample extraction liquor, the sample extraction liquor is added into the aptamer biosensor and evenly mixed, and incubation is performed; 3, supernatant liquor is separated, and an excessive sucrose solution is added for a reaction; 4, quantitative detection is performed through a glucometer. The method for quantitatively detecting aflatoxin B1 in food by combining the aptamer biosensor with the glucometer is easy and convenient to implement, good in specificity and repeatability and high in sensitivity and provides a new means for quantitatively detecting aflatoxin B1.

This invention relates to preparing method for a kind of dielectric / magnet composite wave-absorbing powder in the field of composite material technology. It is prepared through these steps: (1) Prepartion for magnetic powder: mix nitrate aqueous solution that contains ferrite with sucrose solution, heat to get usher of the ferrite, treat the usher to get magnetic powder; (2) Surface treatment of the magnetic powder: add the upper magnetic powder into organic solution, pouring the liquid out after agitating, and the magnetic powder with amino function group is got; (3) originally deposit dielectric / magnet composite material; (4)After finishing above reation, remove the vessel from ice bath or water bath, suct, firstly wash some times by much deion water, then wash by ethanol or acetone until colature has no colour or no deposition when testing by diluted silver nitrite, lastly dry the wet powder and dielectric / magnet composite powder is generated. The electromechanical parameter of the composite powder is adjustable. The composite powder has homogeneous construction, which reinforces its wave-absorbing mechanism.

The invention belongs to the technological field of environmentally-friendly water treatment, in particular to an enzymology method for preparing polysaccharide bio-flocculant. In the method, sucrose solution with different concentrations of substrate is prepared according to the requirements of products with different molecular weights; and then 1 percent of calcium chloride solution is added and 30 percent of acetic acid buffer solution is used for regulating the pH to be 5.2 to 5.4; the amount of enzyme added is regulated and the pH is regulated to range from 5.2 to 5.4; simultaneously, reaction conditions of temperature, stirring speed, reaction time and the like are controlled; termination is determined to stop the reaction by measuring kinetic viscosity, and then ethanol, methanol or isopropanol is adopted for precipitation, or a ultrafiltration membrane system is used for separation and purification so that various technological indexes of products with different molecular weights can fully reach the quality standard of international market on a novel polysaccharide bioflocculant. A controllable molecular weight can be synthesized by the method, particularly the polysaccharide bioflocculant with ultra-high molecular weight and also can be used for large scale industrialized production.

A method for separating sucrose and a second dissolved component from a sucrose-containing solution, preferably a beet-derived sucrose-containing solution, wherein the solution is subjected to a first fractionation by a continuous or sequential chromatographic simulated moving bed process to yield a sucrose-enriched fraction and a fraction enriched with the second dissolved component. The resulting fraction enriched with the second component is subjected to a second chromatographic fractionation that is a simulated moving bed or batch type process, to yield a second sucrose-enriched fraction and a second fraction enriched with the second dissolved component wherein both fractions have an improved yield or purity.

The invention discloses a method for synthesizing dihydric alcohol and polyhydric alcohol, comprising the following steps of: firstly reacting an aqueous sucrose solution with the pH value of 11-14 and the weight percentage content of 10-50 percent for 0.5-2 hours in the presence of a hydrogenolysis catalyst at the reaction temperature of 130-150 DEG C and the hydrogen pressure of 6-8 MPa, wherein sucrose is used as a raw material; then reacting for 0.5-2 hours at the reaction temperature of 220-250 DEG C and the hydrogen pressure of 10-13 MPa; and cooling, filtering and rectifying a reactionproduct to obtain the dihydric alcohol and the polyhydric alcohol, wherein the hydrogenolysis catalyst is Raney nickel, ruthenium / carbon, nickel / ruthenium or CuO-ZnO, the usage amount of the hydrogenolysis catalyst is 15-30 percent relative to the mass of the sucrose, the dihydric alcohol comprises ethylene glycol, propylene glycol and butanediol, and the polyhydric alcohol comprises propanetriol, sorbierite and mannite. The invention is mainly used for synthesizing the dihydric alcohol and the polyhydric alcohol.

Improved processes for the purification of raw or refined sugar, or sucrose, to produce sucrose and sucrose-related products having substantially no inorganic impurities are described, wherein the processes include the use of both cation and anion exchange resins. In accordance with the process, a sucrose starting material, such as refined sugar or invert syrup, is dissolved in water at a temperature sufficient to dissolve the sucrose product and produce a low visicosity sucrose solution having not more than about 76 wt. % solids. Thereafter, the process includes contacting the low viscosity sucrose solution with one or more ion exchange resin beds, which can be separate or mixed, for a time sufficient to yield a highly-purified sucrose product that is substantially free of inorganic elemental impurities.

A method for separating sucrose and a second dissolved component from a sucrose-containing solution, preferably a beet-derived sucrose-containing solution, wherein the solution is subjected to a first fractionation by a continuous or sequential chromatographic simulated moving bed process to yield a sucrose-enriched fraction and a fraction enriched with the second dissolved component. The resulting fraction enriched with the second component is subjected to a second chromatographic fractionation that is a simulated moving bed or batch type process, to yield a second sucrose-enriched fraction and a second fraction enriched with the second dissolved component wherein both fractions have an improved yield or purity.

The invention provides a preparation technology of a novel functional healthy sugar, belonging to the technical field of the preparation of a functional sugar. The technology comprises the following steps of: mixing sucrose or sucrose solution with L-arabinose or L-arabinose solution; and preparing the novel functional healthy sugar by means of reducing temperature, melting and crystallizing. The technology specifically comprises the steps of: mixing the materials; taking the sucrose and the L-arabinose, mixing according to a certain dried material quality ratio, and evenly stirring; melting or concentrating in vacuum: melting the mixture of the two sugars in a heating way or concentrating the mixture of the two sugars in the vacuum to obtain the melted solution of the sucrose and the L-arabinose or the mixed solution with the water content less than 1%; reducing temperature, melting and crystallizing: reducing the temperature of the mixed solution and stirring, so that the crystals of the mixed solution is mutually penetrated and embedded to form into eutectic pasty mixture; and cutting, smashing and sieving: cutting the eutectic pasty mixture, sending the eutectic pasty mixture into a smashing device to smash, and sieving to obtain the novel functional healthy sugar with different mesh numbers.

A method for separating sucrose and a second dissolved component from a sucrose-containing solution, preferably a beet-derived sucrose-containing solution, wherein the solution is subjected to a first fractionation by a continuous or sequential chromatographic simulated moving bed process to yield a sucrose-enriched fraction and a fraction enriched with the second dissolved component. The resulting fraction enriched with the second component is subjected to a second chromatographic fractionation that is a simulated moving bed or batch type process, to yield a second sucrose-enriched fraction and a second fraction enriched with the second dissolved component wherein both fractions have an improved yield or purity.

The invention discloses a method for preparing isomaltulose by polyvinyl alcohol immobilized sucrose isomerase producing bacteria. The polyvinyl alcohol immobilized sucrose isomerase producing bacteria are obtained by adding 5 to 30 weight percent of sucrose isomerase producing wet bacteria into 4 to 50 percent of water solution pulp of an enzyme carrier which is polyvinyl alcohol or / and kieselguhr at 25 to 30 DEG C and uniformly stirring and hardening at -20 DEG C for later use, wherein the ratio of polyvinyl alcohol to kieselguhr is 2:(0.8-1.21). When used, the immobilized enzyme producing bacteria are taken out, naturally unfrozen at room temperature, cut into grains and added into sucrose solution to perform isomerization conversion; then produced sucrose isomerase is used to decompose sucrose remaining in conversion solution; the conversion solution is purified by decolorizing and ion exchange; and an isomaltulose product is obtained by concentration crystallization and centrifugal separation. The method has the advantages that: the operation is simple and efficiency; the material is cheap and readily available; the grains of immobilized bacteria have high strength and are insusceptible to breakage; the activity of sucrose isomerase is high; the service life of the sucrose isomerase is long; and the like. The isomaltulose has high quality, the purity of the isomaltulose is over 98 percent, the sucrose residue content is less than 0.1, and the isomaltulose is more suitable for preparing high-quality isomalt by hydrogenation.

The invention relates to a method for preparing high-concentration fructose syrup by hydrolyzing sucrose, which comprises the following steps: (1) decolorizing a high-concentration sucrose solution by using acid with dosage accounting for 0.1-1.0% of the high-concentration sucrose solution as the hydrolyst while adding 0.05-0.8% of powder active carbon, and carrying out the reaction at 50-80 DEG C for 15-75 minutes to obtain the high-concentration fructose syrup of which the concentration is more than or equal to 45%, wherein the degree of hydrolysis is more than or equal to 97%; (2) and removing acid radicals and a small amount of pigments in the fructose syrup by using weak-base anion-exchange resin, and removing cations by using a strong-acid cation exchange resin, thereby adjusting the pH value of the fructose syrup. Compared with the exisiting method, the method of the invention is simple and convenient. Through deep research into the hydrolysis conditions, the invention provides the hydrolysis conditions capable of ensuring the hydrolysis effect and reducing the production cost. In addition, the invention carries out the hydrolysis and the decoloring synchronously, thereby ensuring the appearance of the syrup and simplifying the production technology. Then, the acid radicals and the cations in the syrup are removed by using anion and cation exchange resins, thereby obtaining the qualified high-concentration fructose syrup.

The invention discloses a method for preparing a carbon-doped titanium dioxide composite material, comprising the following steps of: fully mixing a TiCl3 solution at the mass concentration of 10%-20% and a sucrose solution at the mass concentration of 1%-10% according to the volume ratio of 1:1-6 with magnetically stirring; placing the mixed solution into a thermostat to perform a reaction at the temperature of 100-150 DEG C for 6-12 hours to hydrolyze TiCl3 and carbonize the sucrose to form an amorphous titanium dioxide-carbon composite material; washing the obtained substance with an organic solvent for 3 to 7 times, and washing with distilled water for 3 to 7 times, followed by drying; crushing the obtained substance, placing the ground substance into an activation furnace, adjusting the temperature to 400-700 DEG C at the heating rate of 3-5 DEG C / min under the protection of nitrogen, calcining at constant temperature for 2-6 hours to produce the needed product. According to the invention, titanium dioxide is embedded in translucent carbon films to form tiny briquettes, thus overcoming the shortcoming that nanometer TiO2 is not easy to settle in a water solution and is difficult to recycle.

The invention relates to an improved ultrasonic-assisted pollen mediated plant genetic transformation method, and aims to obviously increase the setting percentage of the plant fertilized by pollen subjected to ultrasonic treatment, thereby increasing the number of transformants obtained for each treatment. The method comprises the following steps: by using an agrobacterium Ti plasmid carrying an exogenous gene segment, colibacillus plasmid or any other DNA vector as a genetic donor and using plant pollen as a receptor, mixing the pollen and exogenous DNA in a 5-50% sucrose solution subjected to aeration and low-temperature treatment, and transferring the exogenous gene into the receptor pollen under the assisting action of ultrasonic; fertilizing the treated pollen onto the stigma of the plant, and harvesting when the grains become ripe; and in the subsequent growth season, sowing the harvested seeds which are obtained after the fertilization of the transformed pollen, screening the germinated seeds and seedlings, carrying out PCR (Polymerase Chain Reaction) amplification and Southern hybridization on the DNA of a seedling sample, and further determining the transformant. The method provided by the invention does not need tissue culture, does not have species or genotype dependency, and can shorten the genetic transformation breeding time and save the manpower and material resources.

The invention provides a binder-free NiO / C-Ni lithium ion battery negative electrode material and a preparation method therefor. NiO / C uniformly grows on the surface of foamed nickel. The specific preparation process comprises: uniformly mixing de-ionized water with hydrogen peroxide, and fully stirring to obtain a uniform solution; transferring the uniform solution to a liner of a hydrothermal reaction kettle, putting multiple pieces of the foamed nickel into the liner of the hydrothermal reaction kettle, performing a hydrothermal reaction at the temperature of 90-150 DEG C for 12-24 hours, then naturally cooling, taking out the foamed nickel, and completely washing the foamed nickel with the de-ionized water; and putting the foamed nickel into a citrate-glucose solution or a sucrose solution, standing for 5-10 hours, taking out a product, drying the product in a drying oven at the temperature of 70 DEG C, finally sintering the product in an N2 atmosphere at the temperature of 300-400 DEG C for 5 hours, and naturally cooling to obtain an NiO / C-Ni composite structure. The electrode preparation method is simple, low in cost and high in controllability; the NiO / C in the prepared NiO / C-Ni uniformly grows on the surface of the foamed nickel and is in good contact with the foamed nickel; and the prepared NiO / C-Ni electrode is relatively high in charging / discharging capacity and excellent in cycle performance.

This is the preparation method of isomalttulose. The steps includes: (1) the raw material is aqueous sucrose solutions, make it through the stablized alpha-glucosyltransferase reaction column or can to produce the transformation liquid which contains isomaltose and other sugers that transformed from enzyme; (2) make the prepared transformation liquid through de-sugers reactor with yeast immobilization in it, to get rid of the sugers other than isomaltose in transformation liquid; (3) then remove the residua in the acentric transformation liquid and get the clear liquid that contains isomaltose, then catalytic hydrogenation to produce isomalttulose. The content of isomaltose in the transformation liquid can reach more than 98%, then after catalytic hydrogenation, the obtained isomalttulose can increase the utilization rate of sucrose. Besides, it can also save energy and increase productivity.