74 results about "Sucrose solution" patented technology

The invention discloses an extraction and purification method and application of plant-derived exosome-like nanoparticles, and relates to the technical field of plant exosome preparation. The extraction and purification method comprises the following steps: washing fresh ginseng, airing for later use, adding a proper amount of buffer solution, mixing, breaking walls, filtering, centrifuging filtrate to obtain supernate, carrying out ultracentrifugation on the supernate to obtain ginseng exosome nano-particle precipitates, resuspending the precipitates, respectively adding pure water sucrose solutions with different concentrations, and carrying out ultracentrifugation to obtain ginseng exosome nano-particles; and re-suspending the target strip, and carrying out ultracentrifugation to remove redundant cane sugar so as to obtain the purified ginseng exosome-like nano-particles. The ginseng exosome-like nano-particles disclosed by the invention have a remarkable effect when being applied to cosmetics or medicines with skin photoaging resistance, skin oxidation resistance and wrinkle resistance. In an anti-UVB (Ultraviolet B) induced cell injury experiment, the ginseng exosome-like nano-particles have an excellent anti-UVB induced cell injury effect.

The invention discloses an acer elegans cross breeding method based on flowering phase difference regulation and control, and belongs to the technical field of garden plant breeding. The method comprises the following steps: selecting an acer elegans variety of which the natural early flowering phase difference is greater than or equal to 10 days and the genetic distance is greater than or equal to 0.5 as a cross parent, carrying out low-temperature refrigeration and shading blue light supplementing treatment on a female parent to delay the flowering phase, and carrying out prolonged illumination, red light supplementing and hormone and nutrient solution spraying on a male parent to advance the flowering phase; male parent pollen is collected through a vacuum negative pressure adsorption device and stored in a closed container, a sucrose solution is sprayed to female parent stigmas before pollination, and repeated pollination is conducted through a directional nozzle at 9-11 o'clock every day; carrying out stratification treatment and temperature-difference temperature-control germination on the hybrid seeds, and carrying out light-temperature regulation and control seedling culture after sowing; the molecular marker is used for verifying the hybridization authenticity and target traits, phenotype determination is carried out on three-year-old seedlings to complete breeding, the hybridization obstacle caused by asynchronous flowering phases of the caenorhabditis elegans varieties is solved through the flowering phase regulation and control technology, and the hybridization success rate is increased.

The invention relates to an extraction method, a modification method and application of dendrobium nobile outer vesicles. The method comprises the following steps: placing dendrobium nobile leaves in a phosphate buffer solution, crushing and separating to ensure that plant cells are fully broken and outer vesicles are released; then a differential centrifugation method is combined with sucrose gradient centrifugation, finally, the outer vesicles in a sucrose solution with the mass concentration of 30-45% are selected, and the extracted dendrobium nobile outer vesicles are moderate in mass and high in purity. Then cholesterol structural analogues such as sterol compounds and triterpenoid saponin active molecules are embedded into a phospholipid bilayer of the dendrobium nobile outer vesicles to modify the dendrobium nobile outer vesicles, and the modification strategy can effectively improve the stability of the dendrobium nobile outer vesicles in a complex physiological environment; and the treatment effect can be enhanced through the synergism of active molecules and endogenous components of the vesicles.

The invention relates to the field of battery materials, and discloses a preparation method of a porous hard carbon material and application of the porous hard carbon material in a sodium-ion battery, the preparation method of the material comprises the following steps: S1, preparing a sucrose solution by taking sucrose as a carbon source, carrying out hydrothermal reaction treatment on the sucrose solution, and then filtering and separating to obtain a hard carbon precursor; s2, dissolving zinc salt with distilled water to prepare a solution, adding the hard carbon precursor obtained in S1 into the solution, then adding absolute ethyl alcohol into the solution, mixing and stirring at normal temperature, finally heating to evaporate the solvent to dryness, and fully grinding to obtain a uniformly mixed material containing the zinc salt; and S3, putting the material obtained in the S2 into a furnace body, heating to 600 DEG C under a protective atmosphere, carrying out primary carbonization, then heating to 1300 DEG C, carrying out high-temperature carbonization, cooling, taking out, and uniformly grinding, so as to obtain the porous hard carbon material. The material provided by the invention has the characteristics of convenient preparation method, high first-week coulombic efficiency and high specific capacity.

The invention provides a preparation method of an exosome antibody microsphere conjugate, which belongs to the technical field of exosomes, and comprises the following steps: preparing a carboxyl polystyrene microsphere suspension with the particle size of 50 nanometers, and ensuring that the size of the microsphere is stabilized in a range of 50 + / -5 nanometers by optimizing centrifugation and cross-linking conditions. And then coupling the exosome marker antibody with the microspheres, and closing unreacted active groups. A mathematical model is adopted to optimize purification process parameters for purification. A dynamic light scattering and transmission electron microscope is utilized to characterize coupling physics properties, and a conjugate meeting requirements is screened. And finally, obtaining fluffy porous exosome antibody microsphere conjugate freeze-dried powder under the protection of a sucrose solution by adopting a vacuum freeze-drying method. The method solves the technical problems that in an existing exosome microsphere conjugate preparation process, centrifugation and purification parameters are often set according to artificial experience, optimization is difficult to obtain, and the quality of the exosome microsphere conjugate is affected.

The invention provides Saccharomyces cerevisiae for producing squalene as well as a screening method and application of the Saccharomyces cerevisiae. The method comprises the following steps: culturing a Saccharomyces cerevisiae engineering strain ySC782 for synthesizing squalene to obtain a to-be-mutagenized bacterial solution, carrying out ARTP mutagenesis on the to-be-mutagenized bacterial solution, adding the to-be-mutagenized bacterial solution into a YPD liquid culture medium, culturing to obtain a resuscitation bacterial solution, adding the resuscitation bacterial solution into a sucrose solution with gradient concentration distribution, screening, and repeating the ARTP mutagenesis-sucrose solution screening step to obtain the squalene mutant strain. And finally, the saccharomyces cerevisiae strain ySC782-M5 is obtained through screening, and the saccharomyces cerevisiae strain ySC782-M5 is obtained through screening. The yield of squalene produced by the saccharomyces cerevisiae ySC782-M5 reaches 49.83 g / L, so that a solution is provided for breaking through the yield bottleneck of squalene synthesized by yeast.

The invention belongs to the technical field of medicine, and particularly relates to an aprepitant nanocrystalline composition and a preparation method and application thereof.The aprepitant and a sucrose solution are mixed, a stabilizer is added to obtain a solution to be ground, the solution to be ground is ground through a wet grinding process, and the aprepitant nanocrystalline composition is obtained; uniformly mixing the suspension with a spray drying protective agent and a flow aid to obtain a mixed solution, and drying and curing the mixed solution by adopting a spray dryer to obtain the aprepitant nanocrystal composition in the form of a dry suspension, and directly spray-drying the suspension, uniformly mixing the suspension with a skeleton agent and an adhesive, adding water to a constant volume, carrying out vacuum degassing, and carrying out pre-freezing and freeze-drying to obtain the freeze-dried orally disintegrating tablet type aprepitant nanocrystal composition.

The invention discloses a composition containing ginseng exosome and super-elastic protein and application of the composition, and belongs to the technical field of biology. The composition containing the ginseng exosome and the super-elastic protein is prepared by compounding the ginseng exosome and the super-elastic protein according to the weight ratio of 10: (100-150), the ginseng exosome is prepared by the following method: extracting the ginseng exosome from ginseng root tissues by using a differential centrifugation method to obtain a ginseng exosome crude extract; adding sucrose solutions with different concentrations into the ginseng exosome crude extract, and performing ultra-high-speed centrifugal purification to obtain purified ginseng exosome; the amino acid sequence of the super-elastic protein is as shown in SEQ ID NO. 3. According to the invention, the ginseng exosome and the super-elastic protein are combined for use for the first time, and both the ginseng exosome and the super-elastic protein have the effect of promoting cell proliferation, so that the use safety is high; the ginseng exosome and the super-elastic protein can synergistically improve the skin tightening and anti-wrinkle effects and the skin injury repairing effect.

The utility model discloses a drying machine for sucralose production, which relates to the technical field of sucralose production and comprises a shell, side plates are fixedly connected to two sides of the inside of the shell, the shell forms a wrapping structure for the tops and two sides of the side plates, exhaust windows are arranged on one side of the shell, a drying structure is arranged between the two exhaust windows, and the two exhaust windows are connected with the drying structure. The drying structure comprises four transmission rollers rotationally connected between the two exhaust windows, the outer sides of the four transmission rollers are rotationally connected with a conveying belt, the conveying belt is of a trapezoidal structure, the top of the conveying belt is arranged to be an inclined face, and the exhaust windows are located at the lower end of the top of the conveying belt. According to the drying machine for sucralose production, heat is diffused and evenly projected to a sucralose solution on the conveying belt through the heating fins, a stable heating environment is formed, the heating fins and the inclined face of the top of the conveying belt are distributed in parallel, it is guaranteed that heating is concentrated and even, and the common problem of local overheating or uneven drying in traditional drying equipment is solved.

The invention relates to a germplasm cryopreservation method suitable for bait microalgae, and belongs to the field of algae preservation, and the method comprises the following steps: carrying out low-temperature domestication on microalgae in a logarithmic phase, and centrifugally collecting algae mud; mixing the algae mud with a protective solution for pretreatment; after pretreatment, mixing with a cryoprotectant with specific composition, carrying out gradient cooling, and storing at-80 DEG C; during thawing, the cryopreservation tube is quickly thawed in a water bath, and the cryoprotectant is subjected to gradient elution through a sucrose solution. The method successfully realizes long-term stable preservation of various bait microalgae at-80 DEG C by optimizing a cryoprotectant combination and optimizing key parameters such as a protective solution, balance time and thawing temperature, has high survival rate and stable physiological traits after resuscitation, and is low in cost, convenient to operate and suitable for industrial production. And a new technical scheme is provided for protection and utilization of microalgae germplasm resources.

The invention discloses a method for establishing a bee sleep rhythm injury model, which is characterized by comprising the following steps of: animal selection: selecting a mature bee as an object for establishing the bee sleep rhythm injury model; preparing a caffeine cane sugar solution: dissolving caffeine in the sterile cane sugar solution to prepare the caffeine cane sugar solution; and model establishment: performing light-dark alternate culture on mature bees and feeding the bees with the caffeine sucrose solution to establish the bee sleep rhythm injury model. The invention further discloses application of the germ bee sleep rhythm injury model and / or the sleep rhythm injury sterile bee model established by the method. The method is easy and convenient to operate, low in cost, short in modeling period, remarkable in sleep rhythm injury symptom and adjustable in disease degree, and the established bee sleep rhythm injury model can be used for screening new methods or new drugs for preventing or treating sleep rhythm injury related diseases.

The invention discloses a sucrose-based piezoelectric coating and a sensor preparation method and application thereof. The preparation method comprises the following steps: performing first heat treatment on a sucrose solution to obtain an amorphous sucrose film; and pressing the amorphous sucrose film to promote vertical growth of the seed crystal so as to obtain the sucrose-based piezoelectric coating. The columnar sucrose-based piezoelectric coating can be successfully prepared without large-scale film preparation equipment, the obtained sucrose-based piezoelectric coating has high crystallinity and good piezoelectric performance, meanwhile, based on the melting point of sucrose, the sucrose-based piezoelectric coating and the sensor thereof do not need to be prepared at a high temperature, and the preparation cost is low. The piezoelectric sensor can be formed on the surface of a material with poor temperature tolerance, and piezoelectric sensing detection is realized.

The utility model relates to the technical field of filtering for sucralose decoloration, in particular to a filtering device for sucralose production, which comprises a coarse filtering pipe and a fine filtering pipe which are vertically arranged, the coarse filtering pipe is communicated with the fine filtering pipe through a transverse pipe, and the fine filtering pipe is detachably connected with the transverse pipe. The lower end of the coarse filter pipe is closed, the upper portion of the coarse filter pipe is communicated with a filter screen, the lower end of the fine filter pipe is closed through a baffle, a plurality of water outlet holes are formed in the baffle, filter cloth is laid on the top face of the baffle, and a pressing plate matched with the inner diameter of the suction filter pipe and capable of being arranged above the transverse pipe is arranged above the fine filter pipe. The pressing plate is driven by a driving device to move up and down along the suction filter pipe. By using the sucralose filtering device, the sucralose solution can be secondarily filtered by using the filter screen and the filter cloth, so that the sucralose solution can be prevented from containing carbon powder, and the purity of the sucralose solution is ensured.

The application belongs to the technical field of traditional Chinese medicine exosomes, and particularly relates to mulberry-derived extracellular vesicle particles, a preparation method thereof and application thereof in the preparation of medicines for preventing and treating hyperlipidemia and related symptoms. The mulberry-derived extracellular vesicle particles provided by the application are prepared by using mulberries as raw materials, centrifuging the mulberries at different centrifugal speeds after juice extraction, and then performing density gradient centrifugation with a sucrose solution with a specific density. Experimental research shows that the mulberry-derived extracellular vesicle particles can significantly reduce total cholesterol, triglyceride and low-density lipoprotein in blood, inhibit the formation of aortic plaques caused by hyperlipidemia and lipid deposition in the liver, and are safe and non-toxic, and therefore can be used for the preparation of medicines for preventing and treating hyperlipidemia and related symptoms.

The application provides a method for extracting and application of broccoli exosome, and the extraction method is as follows: (1) cut the washed broccoli into pieces, mix with PBS buffer, beat pulp, filter, and take the supernatant; (2) centrifuge the supernatant obtained in step 1 for multiple times; (3) ultracentrifuge the supernatant obtained in step 2 after multiple centrifugations, reserve the precipitate, and resuspend with PBS buffer; (4) ultracentrifuge the suspension in step 3 in sucrose solutions with different concentrations, and collect and combine the bands between different concentration sucrose solution layers; (5) mix the bands obtained in step 4 with PBS buffer, ultracentrifuge, remove sucrose, and the obtained precipitate is broccoli exosome. Meanwhile, the application also provides application of the broccoli exosome obtained by the above extraction method in preparation of a medicine for relieving constipation.

The invention discloses an embryo freezing resuscitation reagent and a resuscitation method, and belongs to the technical field of embryo freezing resuscitation. According to the embryo freezing resuscitation reagent provided by the invention, resuscitation liquid is improved, and compared with an existing 1M sucrose solution, the same resuscitation effect can be achieved only by adopting a 0.3-035 M sucrose solution. The reagent concentration is remarkably reduced, and the cost can be effectively reduced. The invention further provides a rapid resuscitation method matched with the embryo freezing resuscitation reagent, the whole resuscitation process of one embryo is about 2 min, 20 FET periods can be completed only about 40 min, the working efficiency is greatly improved, the operation process is simplified, the waiting time of a patient is shortened, and the satisfaction degree of the patient is improved. The embodiment proves that the rapid resuscitation scheme has no influence on the survival of the blastocyst, the survival rate of the resuscitated blastocyst reaches 100%, the continuous pregnancy rate can be effectively increased, and the abortion rate is reduced.

This invention relates to the field of molecular biology, and more specifically, to a novel application of *Tea glomerata* exovesicles, namely, their application in the preparation of anti-inflammatory drugs, specifically in drugs for treating acute pneumonia. The method for preparing *Tea glomerata* exovesicles includes the following steps: Fresh *Tea glomerata* slices are juiced, and the juice is collected. The juice is centrifuged sequentially, and the supernatant from the third centrifugation is collected and centrifuged at 120,000-140,000g for 50-60 minutes to obtain a crude extract. The crude extract is added to a gradient concentration sucrose solution, centrifuged, and a solution containing 30%-45% bands is collected. This solution is then centrifuged at 150,000-160,000g for 50-60 minutes to obtain the *Tea glomerata* exovesicles. The method of this invention can yield *Tea glomerata* exovesicles that meet standards and have stable quality.

The present application relates to a kind of complex component radioactive waste liquid processing method, steps are as follows: organic matter is contained in waste liquid and sodium hydroxide and sucrose solution is added to pretreat and carry out oxidation decomposition treatment;pH value is adjusted, steam heating is carried out, ammonia in waste liquid is recycled under the condition of high gravity state and is rotated and separated, and after the pH value of deamination waste liquid is adjusted, it is input into first reverse osmosis unit, and is concentrated in circulation, and the concentrated first concentrated phase is carried out rotary evaporation and crystallization, and waste liquid steam and first dilute phase are sent to second reverse osmosis unit and are concentrated in circulation, and the obtained second concentrated phase is recycled;Second dilute phase is discharged or it is input into reverse osmosis+EDI unit and is deeply treated and reused.The present application designs the process flow of preferentially treating waste liquid containing organic matter, and then treating waste liquid not containing organic matter, forms simple and efficient process route;With the advantages of efficient and clear, purification is thorough, easy to operate, simple and reliable, effectively solve the purification treatment problem of complex component radioactive waste liquid.

The invention relates to the technical field of extraction of fresh idesia polycarpa fruits, in particular to a preparation method of high-stability phenol-rich oil from fresh idesia polycarpa flesh. The method comprises the following steps: adjusting the pH value of pulp of fresh idesia polycarpa fruits to 4-7, then adding a first enzyme seed and a second enzyme seed, extracting for 0.5-4 hours at the temperature of 30-50 DEG C, centrifuging, and collecting a first emulsion; the first enzyme comprises pectinase, and further comprises at least one of hemicellulase and cellulase; the second enzyme is composed of tannase and glycosidase; and washing the first emulsion with water or a sucrose aqueous solution, centrifuging, and collecting a second emulsion to obtain the oil body. Through simultaneous enzymolysis of the first enzyme and the second enzyme, free polyphenol is generated in situ around the oil body, interaction of the oil body and polyphenol is promoted, part of polyphenol, protein and pectin are combined on the surface of the oil body, part of weak-polarity polyphenol enters the oil body, and the physical and chemical stability of the oil body is synergistically improved.

The application discloses an active dye adsorbent and a preparation method thereof, and an environmentally-friendly dyeing method for jean fabric, and belongs to the field of textiles. The preparation method comprises the following steps: dissolving sucrose and sodium periodate in water, adjusting the mixed solution to be acidic, adding a precipitant after reaction, and filtering to obtain a formyl sucrose solution; dissolving chitosan in an acetic acid solution to obtain a chitosan solution; mixing the formyl sucrose solution and the chitosan solution, stirring, and filtering to obtain a solid active dye adsorbent. The adsorbent has good adsorption capacity for active blue 19 in an acidic environment, can elute the active blue 19 in an alkaline environment, and has good reusability, so that the active dye and the adsorbent can be reused, the environmentally-friendly dyeing method for jean fabric is applied, waste water can also have value, waste of the active dye can be reduced, subsequent waste water treatment pressure can be reduced, and waste water treatment cost can be reduced.

The present invention discloses a calcium hydroxide content detection apparatus, and relates to the technical field of calcium hydroxide content detection, the calcium hydroxide content detection apparatus comprises an identification assembly for adding water and a hydrochloric acid standard titration solution into a sample, the identification assembly comprises a camera, and the camera performs photographing analysis on the color change of the sample, by comparing an HSV color space, when the sample becomes colorless is judged, meanwhile, the usage amount of a hydrochloric acid standard titration solution is determined, the content of calcium hydroxide in the sample is calculated, and the sample is placed in a stable light environment by the recognition assembly in the analysis process, so that the accuracy of photographing analysis of the sample is improved; the device is also provided with a transfer assembly and a stirring assembly, the transfer assembly drives the sample to move to a working position and shakes up the transfer assembly, and the stirring assembly stirs the sample added with the sucrose solution, so that the detection efficiency and accuracy are improved.

The invention discloses a caffeic acid adsorbent based on cycleanine polymer as well as a preparation method and application thereof, the adsorbent is prepared by taking cycleanine as a functional monomer through cross-linking polymerization with tri (bromomethyl) benzene, and the adsorbent has a specific porous structure and surface chemical properties. The preparation method comprises the steps of reflux reaction in an acetonitrile solvent, washing, drying and the like. During application, the adsorbent is added into a sucrose solution, and phenolic substances such as caffeic acid and the like can be efficiently removed under optimized conditions. The adsorption capacity of the adsorbent to caffeic acid is remarkably higher than that of a traditional material, the adsorbent can be regenerated and reused through alkali washing, and the technical problems that an existing clarification process is low in phenolic substance removal rate and secondary pollution is generated are solved. The technology can be embedded into an existing production line of a sugar factory, clean production is achieved while the quality of the white granulated sugar is improved, and the technology has important application value.

The invention provides a preparation method of decellularized omentum majus, and relates to the technical field of biomedical materials.The method comprises the steps that in a closed container, omentum majus subjected to freeze-drying treatment is placed in a mixed solvent to be subjected to degreasing treatment, and a first intermediate is obtained; performing vacuum pulse treatment on the closed container in the degreasing treatment process; cleaning the first intermediate to obtain a second intermediate; performing circulating oscillation treatment on the second intermediate for at least one time to obtain a third intermediate; the third intermediate is cleaned in a phosphate buffer solution and then placed in a compound enzyme solution containing pancreatin and deoxyribonuclease I to be subjected to oscillation treatment, and a fourth intermediate is obtained; the fourth intermediate is sequentially washed with deionized water and a phosphate buffer solution and then placed in a sucrose solution to be soaked, then freeze-drying and sterilization are sequentially conducted, and the decellularized omentum majus is obtained. By adopting the method disclosed by the invention, the decellularized omentum majus with high purity, low residue and complete structure can be obtained.

The invention discloses an activated carbon adsorbent for industrial sewage treatment and a preparation method thereof, and relates to the technical field of sewage treatment. The method comprises the following steps: 1) adding activated carbon into a phosphoric acid solution for dipping treatment to obtain pretreated activated carbon; 2) adding the pretreated activated carbon into a sucrose solution, carrying out hydrothermal reaction, and activating with potassium hydroxide to obtain modified activated carbon; 2, dispersing the modified activated carbon in deionized water, adding the modified polyacrylamide, and stirring for 1-2 hours; adding sodium alginate and an EDC-NHS cross-linking agent, and stirring for 18 to 24 hours; dropwise adding an aqueous solution of calcium nitrate, and standing; and washing, freeze-drying, grinding and sieving to obtain the activated carbon adsorbent. The activated carbon adsorbent prepared by the invention has good purification capacity on cyanogen-containing industrial wastewater.

The invention discloses an inosine oral solution and a production process thereof, and relates to the technical field of oral solution production, and the production process comprises the following steps: S1, dissolving sucrose in purified water to obtain a sucrose solution; s2, taking inosine, sodium benzoate and stevioside, adding water for dissolving to obtain a mixed solution, then adding the composite adsorption microspheres into the mixed solution, filtering, uniformly mixing the filtrate with a sucrose solution, continuously adding purified water, and meanwhile, adjusting the pH value by adopting sodium hydroxide; filtering and filling; the preparation method of the composite adsorption microspheres comprises the following steps: mixing activated carbon, vermiculite and magnesium lithium silicate, uniformly grinding to obtain a pretreated raw material, adding the pretreated raw material into silica sol, granulating, and then performing electrode treatment to obtain the composite adsorption microspheres. Adsorption treatment is carried out on the mixed solution, and the composite adsorption microspheres with specific composition are adopted, so that impurities in inosine can be further effectively removed, and the influence of the impurities on the stability of inosine is reduced.

The present invention discloses a method for preparing broccoli sprout powder rich in glucoraphanin and sulforaphane, belonging to the field of food processing. The broccoli sprout powder preparation method comprises the following steps: screening broccoli seeds, washing, disinfecting, and soaking them, then placing them on a tray lined with filter paper. At an ambient temperature of 25-27°C, the seeds are first germinated with distilled water for 1-2 days, then sprayed with a sucrose solution every 8-12 hours, and allowed to germinate under natural light. The sprouts are harvested after germination for 4-7 days. The harvested sprouts are first steamed, immediately cooled to room temperature, and then soaked in a zinc sulfate solution. After removal and grinding, the pulp is placed in 2 volumes of distilled water and hydrolyzed with endogenous enzymes at 35-40°C. The hydrolyzed broccoli sprouts are freeze-dried and pulverized to obtain the broccoli sprout powder. This broccoli sprout powder is rich in glucoraphanin and sulforaphane and has high nutritional value.

The invention belongs to the technical field of food processing, and particularly relates to a method for improving hardness of canned peaches. The invention provides a method for improving hardness of canned peaches. The method comprises the following preparation steps: S1, removing peach hair from yellow-meat wild peaches; s2, soaking peaches in alkali liquor, peeling the peaches, and cleaning the peaches until the peaches are neutral; s3, denucleating the peaches, cutting the peaches into petals to obtain peach petals, soaking the peach petals in a low-esterification-degree wild peach pectin-cane sugar solution, and carrying out electromagnetic treatment; and S4, soaking the peach petals in a calcium ion solution, carrying out vacuum treatment, and then cleaning the peach petals with deionized water to obtain the peach petals. The natural low-esterification-degree pectin is added into the sucrose solution, the characteristic that the low-esterification-degree pectin is combined with calcium ions to form gel under the acidic condition is utilized, and electromagnetic treatment is assisted to promote combination of the low-esterification-degree pectin and the calcium ions, so that the problem that the hardness of the yellow peaches for canning is reduced in the processing process is effectively solved; meanwhile, the integrity of the pulp tissue structure is protected, and the natural flavor and nutritional ingredients are effectively reserved.

The invention discloses a non-steroidal anti-inflammatory drug suspension drop and a preparation method thereof. The suspension drop comprises ibuprofen, xanthan gum, pregelatinized starch, glycerin, sucrose, anhydrous citric acid, sodium benzoate, polysorbate 80, strawberry essence and water. The preparation method comprises the following steps: 1) sieving ibuprofen with a 20-mesh sieve for later use; the preparation method comprises the following steps: adding polysorbate 80 into purified water, stirring and dissolving, adding ibuprofen according to the prescription amount, and stirring and dispersing uniformly; 2) adding 100 DEG C purified water and glycerol into the liquid preparation tank, and stirring and mixing; (3) starting a rotor pump and a homogenizing pump to form internal circulation in the liquid preparation tank, opening a ball valve of a charging hopper, adding xanthan gum and pregelatinized starch, closing the valve of the hopper, and homogenizing and stirring until the step (6) is finished; (4) weighing cane sugar, adding the cane sugar into boiling water at 100 DEG C, and stirring for dissolving; 5) continuously stirring in the liquid preparation tank, adding a sucrose solution, washing the container with water, and adding into the liquid preparation tank; 6) adding anhydrous citric acid and sodium benzoate into the liquid preparation tank, and stirring for more than 1 hour after the steps 3-6 are finished; 7) closing the homogenizing pump, adding an ibuprofen dispersion liquid into the liquid preparation tank, washing the dispersion container with water for several times, and stirring; (8) adding essence into the liquid preparation tank, and stirring; 9) adding the total amount of purified water, and stirring; and 10) filling. The drops with good dispersibility and good stability are obtained by screening the prescription and optimizing the preparation process.

The invention discloses beta-cyclodextrin as well as a preparation method and application thereof by taking sucrose as a substrate, and belongs to the technical field of cyclodextrin production. The method comprises the following steps: by taking sucrose as a raw material, firstly adding a sodium citrate buffer solution to prepare a sucrose solution, regulating the temperature and the pH value of a feed liquid, adding a sucrose glucosyltransferase mutant and a cyclodextrin glucosyltransferase mutant to react, transferring and heating the feed liquid after the reaction is finished, adding licolal high-temperature-resistant amylase, and continuously stirring to obtain the sucrose-cyclodextrin glucosyltransferase mutant. The preparation method comprises the following steps: preparing beta-cyclodextrin, then decolorizing and filtering the feed liquid through activated carbon, adding n-hexane into the filtrate, crystallizing at room temperature, finally removing n-hexane through evaporation, removing oligosaccharide through filtration, and performing vacuum drying to obtain the beta-cyclodextrin. The invention discloses a preparation method for producing beta-cyclodextrin by adopting multienzyme reaction, the prepared beta-cyclodextrin has good purity and recovery rate, metal ions do not need to be added for assistance, the preparation process is simple, convenient and easy to control, and efficient preparation of the beta-cyclodextrin can be realized.

The present invention discloses a method for constructing a bumblebee model of acute or chronic neuropathy. The method uses newly emerged bumblebees as the subjects for establishing the bumblebee model of neuropathy; acrylamide is dissolved in a sterile sucrose solution to prepare an acrylamide sucrose solution; the newly emerged bumblebees are fed the acrylamide sucrose solution to obtain the bumblebee model of neuropathy; the model constructed by the method of the present invention can be used to screen methods or drugs for preventing or treating neurotoxicity-related diseases, can be applied to explore the specific mechanisms of nervous system-related diseases, and has good application prospects.