98 results about "Sucrose solution" patented technology

The invention discloses an extraction and purification method and application of plant-derived exosome-like nanoparticles, and relates to the technical field of plant exosome preparation. The extraction and purification method comprises the following steps: washing fresh ginseng, airing for later use, adding a proper amount of buffer solution, mixing, breaking walls, filtering, centrifuging filtrate to obtain supernate, carrying out ultracentrifugation on the supernate to obtain ginseng exosome nano-particle precipitates, resuspending the precipitates, respectively adding pure water sucrose solutions with different concentrations, and carrying out ultracentrifugation to obtain ginseng exosome nano-particles; and re-suspending the target strip, and carrying out ultracentrifugation to remove redundant cane sugar so as to obtain the purified ginseng exosome-like nano-particles. The ginseng exosome-like nano-particles disclosed by the invention have a remarkable effect when being applied to cosmetics or medicines with skin photoaging resistance, skin oxidation resistance and wrinkle resistance. In an anti-UVB (Ultraviolet B) induced cell injury experiment, the ginseng exosome-like nano-particles have an excellent anti-UVB induced cell injury effect.

The invention discloses an acer elegans cross breeding method based on flowering phase difference regulation and control, and belongs to the technical field of garden plant breeding. The method comprises the following steps: selecting an acer elegans variety of which the natural early flowering phase difference is greater than or equal to 10 days and the genetic distance is greater than or equal to 0.5 as a cross parent, carrying out low-temperature refrigeration and shading blue light supplementing treatment on a female parent to delay the flowering phase, and carrying out prolonged illumination, red light supplementing and hormone and nutrient solution spraying on a male parent to advance the flowering phase; male parent pollen is collected through a vacuum negative pressure adsorption device and stored in a closed container, a sucrose solution is sprayed to female parent stigmas before pollination, and repeated pollination is conducted through a directional nozzle at 9-11 o'clock every day; carrying out stratification treatment and temperature-difference temperature-control germination on the hybrid seeds, and carrying out light-temperature regulation and control seedling culture after sowing; the molecular marker is used for verifying the hybridization authenticity and target traits, phenotype determination is carried out on three-year-old seedlings to complete breeding, the hybridization obstacle caused by asynchronous flowering phases of the caenorhabditis elegans varieties is solved through the flowering phase regulation and control technology, and the hybridization success rate is increased.

The invention relates to an extraction method, a modification method and application of dendrobium nobile outer vesicles. The method comprises the following steps: placing dendrobium nobile leaves in a phosphate buffer solution, crushing and separating to ensure that plant cells are fully broken and outer vesicles are released; then a differential centrifugation method is combined with sucrose gradient centrifugation, finally, the outer vesicles in a sucrose solution with the mass concentration of 30-45% are selected, and the extracted dendrobium nobile outer vesicles are moderate in mass and high in purity. Then cholesterol structural analogues such as sterol compounds and triterpenoid saponin active molecules are embedded into a phospholipid bilayer of the dendrobium nobile outer vesicles to modify the dendrobium nobile outer vesicles, and the modification strategy can effectively improve the stability of the dendrobium nobile outer vesicles in a complex physiological environment; and the treatment effect can be enhanced through the synergism of active molecules and endogenous components of the vesicles.

The invention relates to the field of battery materials, and discloses a preparation method of a porous hard carbon material and application of the porous hard carbon material in a sodium-ion battery, the preparation method of the material comprises the following steps: S1, preparing a sucrose solution by taking sucrose as a carbon source, carrying out hydrothermal reaction treatment on the sucrose solution, and then filtering and separating to obtain a hard carbon precursor; s2, dissolving zinc salt with distilled water to prepare a solution, adding the hard carbon precursor obtained in S1 into the solution, then adding absolute ethyl alcohol into the solution, mixing and stirring at normal temperature, finally heating to evaporate the solvent to dryness, and fully grinding to obtain a uniformly mixed material containing the zinc salt; and S3, putting the material obtained in the S2 into a furnace body, heating to 600 DEG C under a protective atmosphere, carrying out primary carbonization, then heating to 1300 DEG C, carrying out high-temperature carbonization, cooling, taking out, and uniformly grinding, so as to obtain the porous hard carbon material. The material provided by the invention has the characteristics of convenient preparation method, high first-week coulombic efficiency and high specific capacity.

The invention provides a preparation method of a spherical mesoporous carbon material. The method comprises the following steps: obtaining a sucrose solution; adding sodium polyacrylate into the sucrose solution; adding silica sol into the sucrose solution added with sodium polyacrylate, and uniformly mixing; adding concentrated sulfuric acid into the sucrose solution added with sodium polyacrylate and silica sol, uniformly mixing, and heating for reaction; washing the reaction product to be neutral, and then drying the reaction product; grinding the dried reaction product, and calcining in a nitrogen atmosphere; and eliminating the silicon template in the reaction product to obtain the spherical mesoporous carbon material. The invention also provides a spherical mesoporous carbon material and a method for simultaneously detecting diisopropylnaphthalene, polychlorinated biphenyl and a photoinitiator in paper for cigarettes. According to the preparation method of the spherical mesoporous carbon material, the prepared high-dispersity spherical mesoporous carbon material is developed in pore structure and high in specific surface area, more adsorption sites can be provided, and the mesoporous structure is beneficial to rapid diffusion of the material in a solution.

The invention provides a preparation method of an exosome antibody microsphere conjugate, which belongs to the technical field of exosomes, and comprises the following steps: preparing a carboxyl polystyrene microsphere suspension with the particle size of 50 nanometers, and ensuring that the size of the microsphere is stabilized in a range of 50 + / -5 nanometers by optimizing centrifugation and cross-linking conditions. And then coupling the exosome marker antibody with the microspheres, and closing unreacted active groups. A mathematical model is adopted to optimize purification process parameters for purification. A dynamic light scattering and transmission electron microscope is utilized to characterize coupling physics properties, and a conjugate meeting requirements is screened. And finally, obtaining fluffy porous exosome antibody microsphere conjugate freeze-dried powder under the protection of a sucrose solution by adopting a vacuum freeze-drying method. The method solves the technical problems that in an existing exosome microsphere conjugate preparation process, centrifugation and purification parameters are often set according to artificial experience, optimization is difficult to obtain, and the quality of the exosome microsphere conjugate is affected.

The invention provides Saccharomyces cerevisiae for producing squalene as well as a screening method and application of the Saccharomyces cerevisiae. The method comprises the following steps: culturing a Saccharomyces cerevisiae engineering strain ySC782 for synthesizing squalene to obtain a to-be-mutagenized bacterial solution, carrying out ARTP mutagenesis on the to-be-mutagenized bacterial solution, adding the to-be-mutagenized bacterial solution into a YPD liquid culture medium, culturing to obtain a resuscitation bacterial solution, adding the resuscitation bacterial solution into a sucrose solution with gradient concentration distribution, screening, and repeating the ARTP mutagenesis-sucrose solution screening step to obtain the squalene mutant strain. And finally, the saccharomyces cerevisiae strain ySC782-M5 is obtained through screening, and the saccharomyces cerevisiae strain ySC782-M5 is obtained through screening. The yield of squalene produced by the saccharomyces cerevisiae ySC782-M5 reaches 49.83 g / L, so that a solution is provided for breaking through the yield bottleneck of squalene synthesized by yeast.

The invention discloses a magnetic bead method endotoxin-free plasmid extraction kit, relates to the technical field of plasmid extraction and endotoxin removal, including: solution I, solution II, solution III, magnetic bead liquid I, magnetic bead liquid II, cleaning solution and eluent; solution I includes: sucrose, EDTA, Tris-HCl, NaCl; solution II includes: KO H, SDS, EDTA; solution III includes: potassium acetate, sodium acetate, trehalose, pH is 5-5.6; magnetic bead liquid I includes: NaCl, sodium citrate, magnetic beads I, pH is 5.0-5.5; magnetic bead liquid II includes: guanidine hydrochloride, urea, anhydrous ethanol, isopropanol, magnetic beads II, pH is 5.5-6.5. The present invention prepares an endotoxin-free plasmid extraction kit based on a double magnetic bead method, which has the advantages of convenience, low cost and good safety, can remove endotoxins in the plasmid extraction process, and can also be used to directly remove endotoxins in plasmids, and the obtained plasmids can meet the requirements of molecular biology experiments and cell experiments such as enzyme cutting, sequencing, transfection and virus packaging. At the same time, full automation of endotoxin-free plasmid extraction can be achieved.

The invention belongs to the technical field of medicine, and particularly relates to an aprepitant nanocrystalline composition and a preparation method and application thereof.The aprepitant and a sucrose solution are mixed, a stabilizer is added to obtain a solution to be ground, the solution to be ground is ground through a wet grinding process, and the aprepitant nanocrystalline composition is obtained; uniformly mixing the suspension with a spray drying protective agent and a flow aid to obtain a mixed solution, and drying and curing the mixed solution by adopting a spray dryer to obtain the aprepitant nanocrystal composition in the form of a dry suspension, and directly spray-drying the suspension, uniformly mixing the suspension with a skeleton agent and an adhesive, adding water to a constant volume, carrying out vacuum degassing, and carrying out pre-freezing and freeze-drying to obtain the freeze-dried orally disintegrating tablet type aprepitant nanocrystal composition.

The invention discloses a composition containing ginseng exosome and super-elastic protein and application of the composition, and belongs to the technical field of biology. The composition containing the ginseng exosome and the super-elastic protein is prepared by compounding the ginseng exosome and the super-elastic protein according to the weight ratio of 10: (100-150), the ginseng exosome is prepared by the following method: extracting the ginseng exosome from ginseng root tissues by using a differential centrifugation method to obtain a ginseng exosome crude extract; adding sucrose solutions with different concentrations into the ginseng exosome crude extract, and performing ultra-high-speed centrifugal purification to obtain purified ginseng exosome; the amino acid sequence of the super-elastic protein is as shown in SEQ ID NO. 3. According to the invention, the ginseng exosome and the super-elastic protein are combined for use for the first time, and both the ginseng exosome and the super-elastic protein have the effect of promoting cell proliferation, so that the use safety is high; the ginseng exosome and the super-elastic protein can synergistically improve the skin tightening and anti-wrinkle effects and the skin injury repairing effect.

The utility model discloses a drying machine for sucralose production, which relates to the technical field of sucralose production and comprises a shell, side plates are fixedly connected to two sides of the inside of the shell, the shell forms a wrapping structure for the tops and two sides of the side plates, exhaust windows are arranged on one side of the shell, a drying structure is arranged between the two exhaust windows, and the two exhaust windows are connected with the drying structure. The drying structure comprises four transmission rollers rotationally connected between the two exhaust windows, the outer sides of the four transmission rollers are rotationally connected with a conveying belt, the conveying belt is of a trapezoidal structure, the top of the conveying belt is arranged to be an inclined face, and the exhaust windows are located at the lower end of the top of the conveying belt. According to the drying machine for sucralose production, heat is diffused and evenly projected to a sucralose solution on the conveying belt through the heating fins, a stable heating environment is formed, the heating fins and the inclined face of the top of the conveying belt are distributed in parallel, it is guaranteed that heating is concentrated and even, and the common problem of local overheating or uneven drying in traditional drying equipment is solved.

The invention relates to a germplasm cryopreservation method suitable for bait microalgae, and belongs to the field of algae preservation, and the method comprises the following steps: carrying out low-temperature domestication on microalgae in a logarithmic phase, and centrifugally collecting algae mud; mixing the algae mud with a protective solution for pretreatment; after pretreatment, mixing with a cryoprotectant with specific composition, carrying out gradient cooling, and storing at-80 DEG C; during thawing, the cryopreservation tube is quickly thawed in a water bath, and the cryoprotectant is subjected to gradient elution through a sucrose solution. The method successfully realizes long-term stable preservation of various bait microalgae at-80 DEG C by optimizing a cryoprotectant combination and optimizing key parameters such as a protective solution, balance time and thawing temperature, has high survival rate and stable physiological traits after resuscitation, and is low in cost, convenient to operate and suitable for industrial production. And a new technical scheme is provided for protection and utilization of microalgae germplasm resources.

The invention discloses a method for establishing a bee sleep rhythm injury model, which is characterized by comprising the following steps of: animal selection: selecting a mature bee as an object for establishing the bee sleep rhythm injury model; preparing a caffeine cane sugar solution: dissolving caffeine in the sterile cane sugar solution to prepare the caffeine cane sugar solution; and model establishment: performing light-dark alternate culture on mature bees and feeding the bees with the caffeine sucrose solution to establish the bee sleep rhythm injury model. The invention further discloses application of the germ bee sleep rhythm injury model and / or the sleep rhythm injury sterile bee model established by the method. The method is easy and convenient to operate, low in cost, short in modeling period, remarkable in sleep rhythm injury symptom and adjustable in disease degree, and the established bee sleep rhythm injury model can be used for screening new methods or new drugs for preventing or treating sleep rhythm injury related diseases.

The invention discloses a sucrose-based piezoelectric coating and a sensor preparation method and application thereof. The preparation method comprises the following steps: performing first heat treatment on a sucrose solution to obtain an amorphous sucrose film; and pressing the amorphous sucrose film to promote vertical growth of the seed crystal so as to obtain the sucrose-based piezoelectric coating. The columnar sucrose-based piezoelectric coating can be successfully prepared without large-scale film preparation equipment, the obtained sucrose-based piezoelectric coating has high crystallinity and good piezoelectric performance, meanwhile, based on the melting point of sucrose, the sucrose-based piezoelectric coating and the sensor thereof do not need to be prepared at a high temperature, and the preparation cost is low. The piezoelectric sensor can be formed on the surface of a material with poor temperature tolerance, and piezoelectric sensing detection is realized.

The invention discloses a manufacturing process for preparing liquid syrup from cane sugar, which is characterized in that the addition amount of glacial acetic acid and citric acid, heating temperature and heating time are taken as experimental factors, sensory evaluation is taken as experimental indexes, and process conditions for preparing invert syrup from cane sugar are optimized through single factor and orthogonal experiments. And adding 0.4-0.6% of citric acid and 0.2-0.4% of glacial acetic acid, and heating for 90 minutes at 95 DEG C. The prepared sucrose invert syrup has the optimal taste, and when the syrup is stored at room temperature, the pH is slowly reduced, the sucrose conversion rate is gradually increased, but the sensory quality is not obviously changed. According to the manufacturing process for preparing the liquid syrup from the cane sugar, the invert syrup produced by taking the cane sugar as the raw material has the advantages of good taste, good flavor, single color, small viscosity, stable quality, long shelf life, no sand return phenomenon and the like, blood sugar rise and weight increase caused by eating starch syrup for a long time are avoided, body health is facilitated, and when the invert syrup is applied to milk tea shops, the liquid syrup has good taste. And the requirements of convenient use and consumer health are met.

The invention relates to a preparation method of an isomaltulose crystal. The preparation method of the isomaltulose crystal comprises the following steps: (1) stirring and mixing a sucrose solution and sucrose isomerase; (2) adding an isomaltulose seed crystal, feeding cane sugar, and keeping stirring in the whole process; and (3) performing centrifugal separation to obtain isomaltulose crystals. According to the method, sucrose isomerism is carried out by adopting sucrose isomerase to obtain isomaltulose, isomaltulose crystal seeds are added in the conversion process, meanwhile, the sucrose is fed, the conversion and crystallization processes are carried out at the same time, isomaltulose gradually generated through conversion grows on the crystal seeds at the same time, and the obtained crystals are regular in crystal form, high in purity and uniform in particle size.

The utility model relates to the technical field of filtering for sucralose decoloration, in particular to a filtering device for sucralose production, which comprises a coarse filtering pipe and a fine filtering pipe which are vertically arranged, the coarse filtering pipe is communicated with the fine filtering pipe through a transverse pipe, and the fine filtering pipe is detachably connected with the transverse pipe. The lower end of the coarse filter pipe is closed, the upper portion of the coarse filter pipe is communicated with a filter screen, the lower end of the fine filter pipe is closed through a baffle, a plurality of water outlet holes are formed in the baffle, filter cloth is laid on the top face of the baffle, and a pressing plate matched with the inner diameter of the suction filter pipe and capable of being arranged above the transverse pipe is arranged above the fine filter pipe. The pressing plate is driven by a driving device to move up and down along the suction filter pipe. By using the sucralose filtering device, the sucralose solution can be secondarily filtered by using the filter screen and the filter cloth, so that the sucralose solution can be prevented from containing carbon powder, and the purity of the sucralose solution is ensured.

The application belongs to the technical field of traditional Chinese medicine exosomes, and particularly relates to mulberry-derived extracellular vesicle particles, a preparation method thereof and application thereof in the preparation of medicines for preventing and treating hyperlipidemia and related symptoms. The mulberry-derived extracellular vesicle particles provided by the application are prepared by using mulberries as raw materials, centrifuging the mulberries at different centrifugal speeds after juice extraction, and then performing density gradient centrifugation with a sucrose solution with a specific density. Experimental research shows that the mulberry-derived extracellular vesicle particles can significantly reduce total cholesterol, triglyceride and low-density lipoprotein in blood, inhibit the formation of aortic plaques caused by hyperlipidemia and lipid deposition in the liver, and are safe and non-toxic, and therefore can be used for the preparation of medicines for preventing and treating hyperlipidemia and related symptoms.

The application provides a method for extracting and application of broccoli exosome, and the extraction method is as follows: (1) cut the washed broccoli into pieces, mix with PBS buffer, beat pulp, filter, and take the supernatant; (2) centrifuge the supernatant obtained in step 1 for multiple times; (3) ultracentrifuge the supernatant obtained in step 2 after multiple centrifugations, reserve the precipitate, and resuspend with PBS buffer; (4) ultracentrifuge the suspension in step 3 in sucrose solutions with different concentrations, and collect and combine the bands between different concentration sucrose solution layers; (5) mix the bands obtained in step 4 with PBS buffer, ultracentrifuge, remove sucrose, and the obtained precipitate is broccoli exosome. Meanwhile, the application also provides application of the broccoli exosome obtained by the above extraction method in preparation of a medicine for relieving constipation.

The present invention provides a tetrachlorosucrose synthesis method, and relates to the technical field of chemical industry production, and the tetrachlorosucrose synthesis method comprises the following steps: (1) cooling trichloroethane, adding thionyl chloride, adding a sucrose solution, and carrying out heat preservation to obtain a material A; (2) heating the material A for the first time, preserving heat for the first time, heating for the second time, preserving heat for the second time, heating for the third time, preserving heat for the third time, heating for the fourth time, preserving heat for the fourth time and cooling to obtain a material B; (3) heating for the fifth time, preserving heat for the fifth time, heating again after cooling, and repeating the steps; heating for the sixth time, preserving heat for the sixth time, heating again after cooling, and repeating the steps; heating for the seventh time, preserving heat for the seventh time, cooling and then heating again, and repeating the steps to obtain a material C; (3) cooling the material C, neutralizing, reacting, and adjusting the pH value; and (4) carrying out rotary evaporation, and removing trichloroethane and DMF to obtain the tetrachlorosucrose. The method has the advantages of low cost, high yield, environmental protection and the like.

The invention discloses an embryo freezing resuscitation reagent and a resuscitation method, and belongs to the technical field of embryo freezing resuscitation. According to the embryo freezing resuscitation reagent provided by the invention, resuscitation liquid is improved, and compared with an existing 1M sucrose solution, the same resuscitation effect can be achieved only by adopting a 0.3-035 M sucrose solution. The reagent concentration is remarkably reduced, and the cost can be effectively reduced. The invention further provides a rapid resuscitation method matched with the embryo freezing resuscitation reagent, the whole resuscitation process of one embryo is about 2 min, 20 FET periods can be completed only about 40 min, the working efficiency is greatly improved, the operation process is simplified, the waiting time of a patient is shortened, and the satisfaction degree of the patient is improved. The embodiment proves that the rapid resuscitation scheme has no influence on the survival of the blastocyst, the survival rate of the resuscitated blastocyst reaches 100%, the continuous pregnancy rate can be effectively increased, and the abortion rate is reduced.

This invention relates to the field of molecular biology, and more specifically, to a novel application of *Tea glomerata* exovesicles, namely, their application in the preparation of anti-inflammatory drugs, specifically in drugs for treating acute pneumonia. The method for preparing *Tea glomerata* exovesicles includes the following steps: Fresh *Tea glomerata* slices are juiced, and the juice is collected. The juice is centrifuged sequentially, and the supernatant from the third centrifugation is collected and centrifuged at 120,000-140,000g for 50-60 minutes to obtain a crude extract. The crude extract is added to a gradient concentration sucrose solution, centrifuged, and a solution containing 30%-45% bands is collected. This solution is then centrifuged at 150,000-160,000g for 50-60 minutes to obtain the *Tea glomerata* exovesicles. The method of this invention can yield *Tea glomerata* exovesicles that meet standards and have stable quality.

The present invention belongs to the technical field of pharmaceutical preparations, and specifically relates to a kind of Phyllanthus emblica throat lozenge and a preparation method thereof, wherein the Phyllanthus emblica throat lozenge is a sandwich lozenge, comprising a tablet core and a drug-containing layer coated on the upper and lower sides of the tablet core. The present invention solves the problem of the gravel taste of the Phyllanthus emblica throat lozenge by separately preparing the tablet core and the drug-containing layer, thereby controlling the amount of water-soluble fillers in the two respectively, and at the same time, a certain amount of non-water-soluble fillers in the drug-containing layer both ensure the compressibility of the Phyllanthus emblica throat lozenge and prevent it from being dissolved too quickly in the mouth; the present invention uses sucrose solution as an adhesive, and adds sweeteners and solid flavors, which masks the sour and astringent taste of the Phyllanthus emblica itself and enhances the cool feeling; the present invention prepares the Phyllanthus emblica dry paste powder by low-temperature decompression extraction, greatly increases the content of gallic acid in the dry paste powder, and adopts low-temperature boiling granulation to further reduce the loss of gallic acid, ensure the content of effective ingredients in the Phyllanthus emblica throat lozenge, and improve the efficacy.

The present application relates to a kind of complex component radioactive waste liquid processing method, steps are as follows: organic matter is contained in waste liquid and sodium hydroxide and sucrose solution is added to pretreat and carry out oxidation decomposition treatment;pH value is adjusted, steam heating is carried out, ammonia in waste liquid is recycled under the condition of high gravity state and is rotated and separated, and after the pH value of deamination waste liquid is adjusted, it is input into first reverse osmosis unit, and is concentrated in circulation, and the concentrated first concentrated phase is carried out rotary evaporation and crystallization, and waste liquid steam and first dilute phase are sent to second reverse osmosis unit and are concentrated in circulation, and the obtained second concentrated phase is recycled;Second dilute phase is discharged or it is input into reverse osmosis+EDI unit and is deeply treated and reused.The present application designs the process flow of preferentially treating waste liquid containing organic matter, and then treating waste liquid not containing organic matter, forms simple and efficient process route;With the advantages of efficient and clear, purification is thorough, easy to operate, simple and reliable, effectively solve the purification treatment problem of complex component radioactive waste liquid.

The invention relates to the technical field of extraction of fresh idesia polycarpa fruits, in particular to a preparation method of high-stability phenol-rich oil from fresh idesia polycarpa flesh. The method comprises the following steps: adjusting the pH value of pulp of fresh idesia polycarpa fruits to 4-7, then adding a first enzyme seed and a second enzyme seed, extracting for 0.5-4 hours at the temperature of 30-50 DEG C, centrifuging, and collecting a first emulsion; the first enzyme comprises pectinase, and further comprises at least one of hemicellulase and cellulase; the second enzyme is composed of tannase and glycosidase; and washing the first emulsion with water or a sucrose aqueous solution, centrifuging, and collecting a second emulsion to obtain the oil body. Through simultaneous enzymolysis of the first enzyme and the second enzyme, free polyphenol is generated in situ around the oil body, interaction of the oil body and polyphenol is promoted, part of polyphenol, protein and pectin are combined on the surface of the oil body, part of weak-polarity polyphenol enters the oil body, and the physical and chemical stability of the oil body is synergistically improved.

The application discloses an active dye adsorbent and a preparation method thereof, and an environmentally-friendly dyeing method for jean fabric, and belongs to the field of textiles. The preparation method comprises the following steps: dissolving sucrose and sodium periodate in water, adjusting the mixed solution to be acidic, adding a precipitant after reaction, and filtering to obtain a formyl sucrose solution; dissolving chitosan in an acetic acid solution to obtain a chitosan solution; mixing the formyl sucrose solution and the chitosan solution, stirring, and filtering to obtain a solid active dye adsorbent. The adsorbent has good adsorption capacity for active blue 19 in an acidic environment, can elute the active blue 19 in an alkaline environment, and has good reusability, so that the active dye and the adsorbent can be reused, the environmentally-friendly dyeing method for jean fabric is applied, waste water can also have value, waste of the active dye can be reduced, subsequent waste water treatment pressure can be reduced, and waste water treatment cost can be reduced.

The present invention discloses a calcium hydroxide content detection apparatus, and relates to the technical field of calcium hydroxide content detection, the calcium hydroxide content detection apparatus comprises an identification assembly for adding water and a hydrochloric acid standard titration solution into a sample, the identification assembly comprises a camera, and the camera performs photographing analysis on the color change of the sample, by comparing an HSV color space, when the sample becomes colorless is judged, meanwhile, the usage amount of a hydrochloric acid standard titration solution is determined, the content of calcium hydroxide in the sample is calculated, and the sample is placed in a stable light environment by the recognition assembly in the analysis process, so that the accuracy of photographing analysis of the sample is improved; the device is also provided with a transfer assembly and a stirring assembly, the transfer assembly drives the sample to move to a working position and shakes up the transfer assembly, and the stirring assembly stirs the sample added with the sucrose solution, so that the detection efficiency and accuracy are improved.

The invention discloses a caffeic acid adsorbent based on cycleanine polymer as well as a preparation method and application thereof, the adsorbent is prepared by taking cycleanine as a functional monomer through cross-linking polymerization with tri (bromomethyl) benzene, and the adsorbent has a specific porous structure and surface chemical properties. The preparation method comprises the steps of reflux reaction in an acetonitrile solvent, washing, drying and the like. During application, the adsorbent is added into a sucrose solution, and phenolic substances such as caffeic acid and the like can be efficiently removed under optimized conditions. The adsorption capacity of the adsorbent to caffeic acid is remarkably higher than that of a traditional material, the adsorbent can be regenerated and reused through alkali washing, and the technical problems that an existing clarification process is low in phenolic substance removal rate and secondary pollution is generated are solved. The technology can be embedded into an existing production line of a sugar factory, clean production is achieved while the quality of the white granulated sugar is improved, and the technology has important application value.

The invention relates to the technical field of bioengineering, in particular to a universal method for constructing Ribo-seq sequencing libraries of different tissues and cells of mice and people. The method specifically comprises the following steps: splitting a sample, collecting a splitting product supernatant, adding the splitting product supernatant to the surface of a 30% sucrose solution, centrifuging, re-suspending and precipitating, adding RNase I with the concentration of 128 U / OD, and carrying out enzyme digestion for 0.5 h at the temperature of 25 DEG C; and carrying out RPF extraction, library construction sequencing and data analysis on the enzyme digestion product. The invention aims to construct a method of an improved Ribo-seq sequencing library widely applicable to different tissues and cells of mice and people, condition exploration for different tissues is not needed, the application range is wide, and the experiment difficulty is reduced.

The invention provides a preparation method of decellularized omentum majus, and relates to the technical field of biomedical materials.The method comprises the steps that in a closed container, omentum majus subjected to freeze-drying treatment is placed in a mixed solvent to be subjected to degreasing treatment, and a first intermediate is obtained; performing vacuum pulse treatment on the closed container in the degreasing treatment process; cleaning the first intermediate to obtain a second intermediate; performing circulating oscillation treatment on the second intermediate for at least one time to obtain a third intermediate; the third intermediate is cleaned in a phosphate buffer solution and then placed in a compound enzyme solution containing pancreatin and deoxyribonuclease I to be subjected to oscillation treatment, and a fourth intermediate is obtained; the fourth intermediate is sequentially washed with deionized water and a phosphate buffer solution and then placed in a sucrose solution to be soaked, then freeze-drying and sterilization are sequentially conducted, and the decellularized omentum majus is obtained. By adopting the method disclosed by the invention, the decellularized omentum majus with high purity, low residue and complete structure can be obtained.