Enzymology method for preparing polysaccharide bioflocculant

A biological flocculant and polysaccharide technology, applied in the direction of flocculation/sedimentation water/sewage treatment, fermentation, etc., to achieve excellent quality and good flocculation effect

Active Publication Date: 2009-03-25
威海汉邦生物环保科技股份有限公司
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Problems solved by technology

[0004] The purpose of the present invention is to provide an advanced enzymatic method for preparing polysaccharide bioflocculants against the defects of flocculants currently used in water treatment, so as to industrially synthesize various molecular weights on a large scale under controlled conditions, especially Ultra-high molecular weight polysaccharide bioflocculant to meet the urgent demand for such products in the current market

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  • Enzymology method for preparing polysaccharide bioflocculant

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preparation example Construction

[0023] The preparation of dextran sucrase, including the selection and cultivation of enzyme-producing bacteria, enzyme production, separation and purification, is a relatively complicated and specialized process. For the detailed process, please refer to the Chinese patent "A Syrup Containing Isomaltooligosaccharides and Fructose and Its Preparation Method" (ZL 03100280.3).

[0024] Polysaccharide bioflocculants with different molecular weights can be prepared by changing the substrate concentration, enzyme concentration and reaction conditions. According to the special requirements put forward by several largest water treatment companies in the world, the dynamic viscosity at 25°C is as follows:

[0025] A. High molecular weight: 3000~5000CPS (15% concentration)

[0026] B. Higher molecular weight: 5000~15000CPS (15% concentration)

[0027] C. Ultra-high molecular weight: 30000~45000CPS (15% concentration)

[0028] D. Extremely high molecular weight: 60000~80000CPS (10% c...

Embodiment 1

[0069] Take a clean 2000 ml beaker or a three-neck flask, add 274 grams of high-quality sucrose, then add an appropriate amount of distilled water, stir to dissolve, and adjust the volume to 1000 ml, further add 10 ml of 0.5% calcium chloride solution, stir and mix; Then adjust the pH to 5.2-5.4 with 30% acetic acid buffer. After the above solution was boiled for 15 minutes, it was cooled to 25°C.

[0070] Add 800 IU (ie 17.5 ml) of freshly prepared enzyme solution (the enzyme activity of this batch is 456 IU / ml) and mix well, then adjust the pH to 5.2-5.4 with 10% acetic acid.

[0071] Put the above reactants in a constant temperature water bath at 20-22°C, turn on the stirrer, adjust the stirring speed to 30-40 rpm, and start timing, and then operate in the manner of stirring for 1 hour and stopping for 2 hours, during which the temperature should be Maintain constant and keep the operating environment clean to avoid bacterial contamination.

[0072] After the reaction has...

Embodiment 2

[0076] Take a clean 10-liter glass or stainless steel container, add 1.25 kg of high-quality sucrose, add 3 liters of distilled water, stir to dissolve, then add distilled water to make the solution volume 5 liters, stir and mix well, then add 50 ml of 0.5% calcium chloride solution, stir Mix well, then adjust the pH to 5.2-5.4 with 30% acetic acid buffer, heat and boil for 15 minutes, then cool to room temperature.

[0077] Put the above solution in a constant temperature water bath at 22-24°C and stir for about half an hour. When the internal temperature of the solution is constant within the above range, add 50,000 IU (i.e. 109.6 ml) of enzyme solution (the activity is the same as in Example 1), stir and mix well, and use Adjust the pH to 5.2-5.4 with 10% acetic acid, continue to stir, and adjust the speed to 40-60 rpm. After stirring for 1 hour, stop for half an hour until the viscosity reaches 5,000-15,000, then heat to terminate the reaction. The separation, purification...

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Abstract

The invention belongs to the technological field of environmentally-friendly water treatment, in particular to an enzymology method for preparing polysaccharide bio-flocculant. In the method, sucrose solution with different concentrations of substrate is prepared according to the requirements of products with different molecular weights; and then 1 percent of calcium chloride solution is added and 30 percent of acetic acid buffer solution is used for regulating the pH to be 5.2 to 5.4; the amount of enzyme added is regulated and the pH is regulated to range from 5.2 to 5.4; simultaneously, reaction conditions of temperature, stirring speed, reaction time and the like are controlled; termination is determined to stop the reaction by measuring kinetic viscosity, and then ethanol, methanol or isopropanol is adopted for precipitation, or a ultrafiltration membrane system is used for separation and purification so that various technological indexes of products with different molecular weights can fully reach the quality standard of international market on a novel polysaccharide bioflocculant. A controllable molecular weight can be synthesized by the method, particularly the polysaccharide bioflocculant with ultra-high molecular weight and also can be used for large scale industrialized production.

Description

technical field [0001] The invention belongs to the technical field of environmental protection water treatment, and in particular relates to an enzymatic method for preparing a polysaccharide biological flocculant. Background technique [0002] Water treatment is an important environmental protection issue related to people's production and life. Flocculants are the most commonly used and effective agents in water treatment. Unfortunately, the vast majority of flocculants used at home and abroad are inorganic or organic compounds, many of which have certain toxic and side effects. Therefore, the research and development of non-toxic and harmless bioflocculants without secondary pollution is one of the most important topics in the field of water treatment today. [0003] Research on bioflocculants, including polysaccharide bioflocculants, is quite active and some progress has been made. But generally speaking, it is still in its infancy. In many biosynthetic processes, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C02F1/56
Inventor 庄汉忠
Owner 威海汉邦生物环保科技股份有限公司
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