Method for producing isomaltulose by transforming sucrose by biological enzymatic method

A technology of isomaltulose and bio-enzyme method is applied in the field of bio-enzymatic conversion of sucrose to produce isomaltulose, which can solve the problems of low bacterial cell concentration, large tolerance, insufficient contact between materials and bacterial cells, etc., and achieve High reaction conditions, good product quality, and not easy to infect bacteria

Active Publication Date: 2009-12-02
GUANGXI INVESTMENT GROUP VECTOR BIOTECH
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  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows for quick decomposition and efficient use of sugar solutions while maintaining their clarification capabilities. It also removes impurities from converted liquids effectively without forming large clumps that could affect its final properties like coloration. Additionally, this technique uses specific types of reagents such as saccharides instead of existing methods requiring expensive equipment. Overall, these technical improvements improve the production yield and purity level of isoaltonic acid (IAA).

Problems solved by technology

This patented describes methods for improving the yield and purity of certain substances called betelutathione glue. These technical means include improvements such as separation techniques like filtrations, solvacillification, and moleculum transferring processes.

Method used

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  • Method for producing isomaltulose by transforming sucrose by biological enzymatic method

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Embodiment 1

[0028] Embodiment 1 prepares the technological process of isomaltulose:

[0029] (1) Circulating the sucrose solution through a transformation tank equipped with immobilized cells with α-glucosyltransferase for transformation, the reaction temperature is 26-32°C, and the reaction produces isomaltulose and other miscellaneous sugars containing more than 85% (such as sucrose, glucose, fructose, etc.) conversion solution;

[0030] (2) Circulate the obtained transformation liquid into the reaction tank for removing miscellaneous sugars equipped with immobilized Saccharomyces cerevisiae, and transform by aeration under the condition of 28-32°C for 24-36 hours, until no bubbles are generated to show miscellaneous sugars has been consumed, and the conversion solution of removing miscellaneous sugars is obtained;

[0031] (3) The conversion solution after removing impurities is subjected to ion exchange to remove impurities to obtain a clear liquid containing isomaltulose, and the cl...

Embodiment 2

[0033] Embodiment two prepares the technological process of immobilized Saccharomyces cerevisiae:

[0034] Immobilized Saccharomyces cerevisiae (Saccharomyces cerevisiae) cultivation can adopt the following specific preparation process: a. medium (mass%): 1-3% yeast extract, 2-6% sucrose, 0.05~0.1% defoamer (peanut oil or poly Silicone oil), pH4~7, sterilized at 120~125℃, sterilized for 25~30min. Cool to 32.5-35.5°C, inoculate yeast strains according to the inoculum amount of 2-10%, and cultivate them with an aeration rate of 0.3-10% (air volume per minute: fermentation volume) and a stirring speed of 100-200rpm 48±12h, put in the tank; b. Centrifuge the fermented liquid through a tube centrifuge at 16,000rpm to collect the wet yeast; c. Dissolve the wet yeast with sterile water, filter, and add 7-12% pre-dissolved sodium alginate solution , and make the concentration of wet yeast in the solution 10-20%, mix well; d. put the above yeast emulsion and sodium alginate mixture in...

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Abstract

The invention discloses a method for producing isomaltulose by transforming sucrose by a biological enzymatic method, which comprises the following processes: (1) fully transforming sucrose solution and immobilized cells with alpha-glucosyltransferase in a transformation tank to obtain transformation solution containing isomaltulose and other impurity sugar, wherein the content of the isomaltulose in the transformation solution reaches more than 85 percent; (2) introducing the obtained transformation solution into an impurity sugar removal reaction tank which is filled with immobilized saccharomyces cerevisiae to remove most of the impurity sugar except the isomaltulose in the transformation solution; and (3) obtaining crystals of the isomaltulose from the transformation solution after the impurity sugar is removed through decolorizing and filter pressing, ion exchange, condensation, high-temperature vacuum crystallization, and removing residual impurity sugar and other impurities by centrifugation.

Description

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Claims

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Application Information

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Owner GUANGXI INVESTMENT GROUP VECTOR BIOTECH
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