Fermentation method for producing pullulan polysaccharide
A technology of pullulan polysaccharide and fermentation method, which is applied in the field of microbial fermentation, can solve the problems of long fermentation time and low yield, and achieve the effects of shortening fermentation time, increasing product yield, and small molecular weight range
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Embodiment 1
[0015] (1) Take a ring of Aureobasidium pullulans and insert it into a 500 ml Erlenmeyer flask with 100 ml of seed culture medium. Shake flask seed culture medium, its aqueous solution is composed of (g / L): soybean oil 30.0, KH 2 PO 4 6.0, (NH 4 ) 2 SO 4 0.5, MgSO 4 .7H 2 O0.2, NaCl1.0, FeSO 4 .7H 2 O0.01, yeast extract 0.5, pH value 6.0, sterilized at 121°C for 20 minutes, shake the flask at 28°C, and cultivate it on a shaker with a rotation speed of 240rpm for 48 hours, as the primary seed solution.
[0016] (2) Take 5 milliliters of the primary seed solution and put it into a 500 milliliter Erlenmeyer flask containing 100 milliliters of seed medium. The composition of the shake flask seed medium is the same as that in step 1. Sterilize at 121°C for 20 minutes, and culture the shake flask at 28°C on a shaker with a rotation speed of 240rpm for 24 hours as the secondary seed solution.
[0017] (3) Put the shake flask seeds into a 30-liter fermenter according to the i...
Embodiment 2
[0021] (1) Take a ring of Aureobasidium pullulans and insert it into a 500 ml Erlenmeyer flask with 100 ml of seed culture medium. Shake flask seed medium, its aqueous solution is composed of (g / L): peanut oil 40.0, KH 2 PO 4 5.0, (NH 4 ) 2 SO 4 0.6, MgSO 4 .7H 2 O0.2, NaCl0.8, FeSO 4 .7H 2 O0.01, yeast extract 0.4, pH value 5.5, sterilized at 121°C for 20 minutes, shake the flask at 28°C, and cultivate it on a shaker with a rotation speed of 240rpm for 48 hours, as the primary seed solution.
[0022] (2) Take 5 milliliters of the primary seed solution and put it into a 500 milliliter Erlenmeyer flask containing 100 milliliters of seed medium. The composition of the shake flask seed medium is the same as 1. Sterilize at 121°C for 20 minutes, and culture the shake flask at 28°C on a shaker with a rotation speed of 240rpm for 24 hours as the secondary seed solution.
[0023](3) Put the shake flask seeds into a 30-liter fermenter according to the inoculum amount of 5%. ...
Embodiment 3
[0027] (1) Take a ring of Aureobasidium pullulans and insert it into a 500 ml Erlenmeyer flask with 100 ml of seed culture medium. Shake flask seed medium, its aqueous solution is composed of (g / L): soybean oil 40.0, KH 2 PO 4 6.0, (NH 4 ) 2 SO 4 0.5, MgSO 4 .7H 2 O0.2, NaCl1.0, FeSO 4 .7H 2 O0.01, yeast extract 0.6, pH value 5.5, sterilized at 121°C for 20 minutes, shake the flask at 28°C, and cultivate it on a shaking table with a rotation speed of 240rpm for 48 hours, as the primary seed solution.
[0028] (2) Take 5 milliliters of the primary seed solution and put it into a 500 milliliter Erlenmeyer flask containing 100 milliliters of seed medium. The composition of the shake flask seed medium is the same as 1. Sterilize at 121°C for 20 minutes, and culture the shake flask at 28°C on a shaker with a rotation speed of 240rpm for 24 hours as the secondary seed solution.
[0029] (3) Put the shake flask seeds into a 30-liter fermenter according to the inoculum amount...
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