Cabbage type rape female and male sterile line Bnmfs molecular marker screening primer and method

Abstract

The invention belongs to the technical field of rape breeding and discloses a cabbage type rape female and male sterile line Bnmfs molecular marker screening primer and a method. The sequence of the primer is as shown in SEQ ID NO:1 to SEQ ID NO:22. The molecular marker screening primer disclosed by the invention is a marker that two sides of a sterile gene of a sterile line Bnmfs are tightly interlinked, the interval of sterile genes in cabbage type rape chromosomes is reduced, and a base is made for later gene cloning; the molecular marker screening primer is good in specificity and stability, and in addition a marker screening method is simple to operate; a co-dominance marker is screened, dominant homozygosis, single plants of dominant heterozygosis and recessive homozygosis can be identified and separated, the characteristic that plants are screened according to phenotypes in conventional breeding is overcome, the breeding workload is reduced, the breeding years are reduced, and the breeding progress is accelerated.

Description

technical field [0001] The invention belongs to the technical field of rapeseed breeding, and in particular relates to a primer and a method for screening the male and female sterile line Bnmfs of Brassica napus by using molecular markers. Background technique [0002] In Ganbai interspecific hybrid progeny, the trait of simultaneous male and female abortion was found for the first time, which was controlled by a pair of recessive genes. The sterile line was named Bnmfs, and the gene mapping was carried out. In 2009, Li Ping's research group at Sichuan Agricultural University discovered a male and female sterile rice mutant MFS-Z9, and located it on chromosome 2 of rice. Male and female were found in other crops, such as wheat and soybeans. Sterile plants, but mostly focused on cytological studies. According to the current research, there is no linkage marker for the male and female sterility gene of Brassica napus, and the markers of other crops cannot be applied to this m...

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Problems solved by technology

[0004] Problems and solutions in the prior art: since the obtained male and female sterile mutants are recessive mutations, and both male and female are sterile, only the heterozygous individual plants can be isolated through selfing, and in addition, the obtained The polymorphic markers were verified in 955 sterile individual plants, and there was a high exchange rate, which may be related to F 2 Derived populations have large genetic background noise caused by

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