The invention belongs to the technical field of
molecular breeding of citrus, and particularly relates to a codominant RFP
molecular marker applicable for breeding
citrus grandis with a red-peel characteristic and application of the codominant RFP
molecular marker. Most varieties of
citrus grandis are light yellow in peel and single in color in China; the
citrus grandis with the red-peel characteristic is novel in color and variety, thereby being more attractive to consumers. The method includes: starting from a unique material, the red-peel citrus grandis, using a primer pair
MYB2F / R to amplify the red-peel citrus grandis and common citrus grandis for regulating promoters of
anthocyanin synthesis key
transcription factor CgMYB2 genes so as to obtain two
DNA fragments, then subjecting
DNA polymorphic regions to PCR amplification,
cloning and sequencing, and comparing two obtained
nucleotide sequences to find that one segment of
insertion mutation of 162bp is between the two sequences, resulting in polymorphism of the sequence-characterized amplified region; designing primer pairs RFP F / R1 and RFP F / R2 according to differences of the two
nucleotide sequences prior to PCR amplification to obtain the codominant RFP
molecular marker capable of distinguishing the red-peel citrus grandis from the common citrus grandis (being light yellow in peel). The invention further discloses a method for preparing the molecular marker.