43 results about "Dna polymorphism" patented technology

The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, e.g. aneuploidy. The present invention involves labeling regions of genomic DNA in each cell in said mixed sample with different labels wherein each label is specific to each cell and quantifying the labeled regions of genomic DNA from each cell in the mixed sample. More particularly the invention involves quantifying labeled DNA polymorphisms from each cell in the mixed sample.

The present invention provides a method called genome distribution analysis, which simultaneously scans the nucleic acid sequences (including genome differential sequences, group-specific sequences, and DNA polymorphisms) of various types of biological characteristics in complex biological samples. exist. The invention also includes probes, detection assemblies and related molecules for use in the methods of the invention.

A method for identifying resistance of a rice plant to rice blast is provided. More particularly, a method for identifying resistance of a rice plant to rice blast by testing a genotype of the rice genome using a DNA marker (G271), which is closely linked to a gene controlling the field resistance to rice blast, is disclosed. The disclosed invention allows for evaluation of the field resistance of a rice plant to rice blast using a DNA marker, thereby allowing for a resistant variety to be conveniently and accurately selected. The disclosed invention contributes to reducing the time and labor that have conventionally been required for cross breeding, and is useful in developing novel rice varieties having a high degree of field resistance to rice blast.

The invention discloses a multiple detection method for deoxyribose nucleic acid (DNA) of a genome and a special probe thereof, and provides a single chain DNA molecule. The single chain DNA molecule consists of the following segments sequentially from 5' tail end to 3' tail end: a left side oligonucleotide segment complementary with a 5' end of DNA to be detected, a main body segment and a rightside oligonucleotide segment complementary with a 3' end of the DNA to be detected. Experiments prove that: in the single chain DNA molecule provided by the invention, loci with different markers andalleles are distinguished from one another through a ligase reaction, buckled probes which have different lengths and are successfully connected through polymerase chain reaction (PCR) amplification can simultaneously detect the polymorphism of a plurality of DNA markers through a gel or capillary electrophoretic separation and detection system, high sensitivity and accuracy can be realized, and the requirements of high throughout and low cost are met simultaneously.

The invention provides a bPrimer batch PCR primer design method based on a Primer 3.The method includes the steps that an original sequence of a target DNA sequence is obtained; a high-frequency polymorphic locus in DNA polymorphic data is extracted; the high-frequency polymorphic locus is labeled; an annotated sequence of the labeled high-frequency polymorphic locus is output, and the annotated sequence and the original sequence are identical in basic group length; the annotated sequence is read to generate candidate primers; the candidate primers are screened.The bPrimer batch PCR primer design method based on the Primer 3 can avoid the high-frequency polymorphic locus, reduces amplification failure caused by genetic diversity of target population, can detect specificity of primers in batches, reduces amplification failure caused by nonspecific amplification, primer dimer and other reasons, can be used for evaluating specificity of existing primers, and can automatic segment long target segments.

The invention discloses a method for assisting identification of meat production related traits of sheep and application thereof. The invention provides a method for assisting identification of meat-producing related traits of sheep, which comprises the following steps of: adopting specific primers to carry out PCR amplification on genomic DNA of sheep to be tested, utilizing DNA sequencing and asymmetric PCR; and using the specific primers to amplify the genomic DNA of sheep to be tested. DNA polymorphism detection and rapid typing by SSCP, The 209th nucleotide from 5 'end of PCR amplified product was A or G, and the genotype of sheep was GG or AA. There was a certain correlation between the SNP locus and the meat-producing traits of sheep. The meat-producing performance of sheep with GGgenotype was higher than that of sheep with AA genotype. The invention can be used for early screening of sheep meat production, effectively increasing the meat production of sheep breeding, reducingbreeding cost, and improving the economic benefit of sheep breeding. The method of the invention is simple in operation, low in cost and high in accuracy, and will have important significance for sheep breeding.

The present invention relates to methods, compositions, and kits for identifying biomarkers useful in characterizing biological states. In particular, the invention relates to methods and compositions for molecular characterization of biological states by gene expression profiling. The invention also relates to assessing effects of DNA polymorphisms on regulation of transcription. The biomarkers and polymorphisms identified find use in diagnostic and treatment approaches, e.g., some embodiments of the invention provide methods and kits for detecting bronchogenic carcinoma and risks thereof.

The invention belongs to the technical field of molecular breeding of citrus, and particularly relates to a codominant RFP molecular marker applicable for breeding citrus grandis with a red-peel characteristic and application of the codominant RFP molecular marker. Most varieties of citrus grandis are light yellow in peel and single in color in China; the citrus grandis with the red-peel characteristic is novel in color and variety, thereby being more attractive to consumers. The method includes: starting from a unique material, the red-peel citrus grandis, using a primer pair MYB2F/R to amplify the red-peel citrus grandis and common citrus grandis for regulating promoters of anthocyanin synthesis key transcription factor CgMYB2 genes so as to obtain two DNA fragments, then subjecting DNA polymorphic regions to PCR amplification, cloning and sequencing, and comparing two obtained nucleotide sequences to find that one segment of insertion mutation of 162bp is between the two sequences, resulting in polymorphism of the sequence-characterized amplified region; designing primer pairs RFP F/R1 and RFP F/R2 according to differences of the two nucleotide sequences prior to PCR amplification to obtain the codominant RFP molecular marker capable of distinguishing the red-peel citrus grandis from the common citrus grandis (being light yellow in peel). The invention further discloses a method for preparing the molecular marker.

The invention provides an erigeron breviscapus DNA polymorphism detection method and application thereof. In the invention, an amplified fragment length polymorphism technology is used for erigeron breviscapus DNA polymorphism detection, an optimum primer combination is screened out, and reaction condition is optimized. The method can be effectively applied to the inheritance breeding, the resource protection, the introduction domestication, the breed optimization and the like of erigeron breviscapus.

The invention relates to DNA polymorphisms in sterol regulator element binding proteins (SREBP) that are characteristic of a higher risk of genetic diseases in humans such as hyperchlolesterolemia. The corresponding polymorphisms, especially the polymorphisms on SREBP-1 and SREBP-2 are frequently observed in Alzheimer patients (SREBP-2). They are also characterized by a specific behavior in the therapy of HIV patients with proteas inhibitors and appear to have an influence on the mortality.

The invention provides a method for simultaneously detecting genomic DNA polymorphism and methylation. The method comprises the following steps: performing DNA fragmentation, and digesting the 3' endof the DNA fragment with DNA exonuclease; adding a dNTPs mixture containing 5-hydroxymethylcytosine deoxyribonucleotide, and performing completion the 3' end of the DNA fragment; performing bisulfiteconversion treatment on the modified DNA fragment and connecting an adaptor adapted to a high-throughput sequencing platform, and finally constructing a sequencing library by PCR amplification. By combining a double-end sequencing mode of the high-throughput sequencing platform, genomic DNA polymorphism and methylation detection can be performed simultaneously, and the goal of simultaneous detection of genome and epigenome is achieved.