50 results about "Genotype determination" patented technology

Methods and compositions are provided for predicting whether a subject will comply with an antidepressant treatment regimen. In practicing the subject methods, a subject's serotonin transporter gene-linked polymorphic region (5HTTLPR) is genotyped. Based on the identified genotype, a particular antidepressant treatment regimen suitable for the patient is determined. Also provided are kits for practicing the subject methods.

The invention is applicable to the field of biological engineering and provides a method for detecting mononucleotide polymorphism. The method comprises the following steps: sequencing fragments obtained by high throughput sequencing technology are compared on a referenced genome sequence; the likelihood ratio of various genotypes of the corresponding sites on the genome to be tested is obtained according to the sequencing mass fraction of each basic group in the genome to be tested and obtained by sequencing; the posterior probability of each genotype of each site on the referenced genome is calculated according to the likelihood ratio and the prior probability preset for each genotype, and the genotype which has the highest posterior probability is determined as the most likely right genotype of the corresponding sites on the genome to be tested to obtain the consistent sequence of the genome to be tested; and the sites of the genome to be tested, which are inconsistent with the sequence of the referenced genome in the consistent sequence are detected to obtain the polymorphism sites of the genome to be tested. The embodiment of the invention can achieve a more accurate test result as the influence of the prior probability on mononucleotide polymorphism test result is considered in advance.

The invention discloses a method of detecting the quality of pork. In the method, the gene type of the pig is determined by detecting that the ribonucleotide is C or G on the 451st site on the 51end of the sequence 1 or the 112th site on the 5 (1) end of the sequence 2; and the quality of pork is determined by the gene type; the method of determining the gene type of the pig is as follows: when the ribonucleotide is C on the 451st site on the 5 (1) end of the sequence 1, the homozygote gene type is AA; when the ribonucleotide is G on the 112th site on the 51 end of the sequence 2, the homozygote gene type is BB; the heterozygous gene type is AB; the method of determining the quality of pork via the gene type is as follows: the AA gene type pork tenderness and pH are higher than the AB gene type pork; the AB gene type AB gene type are higher than the BB gene type pork. The method of invention can be applied to detect the tenderness and pH value of the pig which indicate the quality of muscle, which provides a method of accurately and conveniently detecting the hereditary character for the molecular breeding of pig.

The present invention relates to methods and compositions for predicting drug responses. In particular, the present invention provides methods and compositions for determining individualized Warfarin dosages based on genotype of DNA polymorphisms and haplotypes derived from them in the VKORC1 gene.

The present invention relates to methods and compositions for predicting drug responses. In particular, the present invention provides methods and compositions for determining individualized Warfarin dosages based on genotype of DNA polymorphisms and haplotypes derived from them in the VKORC1 gene.

The present invention relates to methods and compositions for predicting drug responses. In particular, the present invention provides methods and compositions for determining individualized Warfarin dosages based on genotype of DNA polymorphisms and haplotypes derived from them in the VKORC1 gene.

The invention provides methods for determining the clinical outcomes for treatment with various treatment regimens available to cancer patients based on genotypes of the patients for genetic polymorphism markers. The invention also provides kits for making the determination.

The present invention provides a method for determining the individual effect of a plurality of genetic marker alleles on udder health, fertility and / or other health of a plurality of reference bovine subjects. The marker effects are employed in another aspect of the invention for determining a genomic estimated breeding value of a bovine subject based on the genotype of said bovine subject by correlating its genotype with the effect of each individual genetic marker allele on udder health, fertility, other health and / or estimated breeding value of the reference bovine subjects. Further provided are methods and computer program products and computer readable media for executing the methods of the invention.

The invention discloses a method for identification or auxiliary identification of day age of a pig at 100kg weight and a special kit of the method. The method comprises the following steps: detecting whether deoxyribonucleotide on the 402nd site of H19 gene of a pig individual is either C or T or both of them, so as to determine whether the genotype of the pig individual is TT or CC or TC; and determining the day age of the pig at 100kg weight according to the genotype of the pig individual: the day age of the pig individual of the TT genotype at 100kg weight is more than that of the pig individual of the CC genotype, and the day age of the pig individual of the CC genotype at 100kg weight is more than that of the pig individual of the TC genotype. Tests prove that the method disclosed by the invention, which is used for identifying the day age of the pig at 100kg weight through single nucleotide polymorphism on the 402nd site of the H19 gene, is consistent with an actual determination result of the day age of the pig at 100kg weight; therefore, the method is of great significance to the selection of excellent pigs.

The invention relates to the technical field of breeding of livestock and poultry, and especially relates to an efficient goat breeding method based on meat properties. The method comprises the following steps: determination on body weight, DNA sample acquisition, primer synthesis, template PCR amplification of a primer, genotype determination and genotyping, sequencing verification on individual PCR products with different genotypes, individual MBLUP heredity assessment, matching and establishing of a family, formation of a core colony through colony continuous-progeny selection, and manual dispersion of excellent genes. By determining body weight and detecting molecular marker genotype, MBLUP technology is employed for evaluating breeding value of breeding goats so as to perform selection, body-weight determination and genotype detection are performed on selected progeny according to a designed selection target, and the MBLUP method is used to perform colony continuous-progeny selection, so that finally a new variety of north Guizhou Ma goat for meat is formed. By taking selection of north Guizhou Ma goat as an example, the body weight of rams and ewes in a selected north Guizhou Ma goat core colony for meat is improved by 14.12 Kg and 9.61 Kg compared with a basic colony.

The present invention discloses an apparatus for detecting gene mutation, and a kit for typing the genotype of pregnant women and fetuses. The apparatus comprises a detection module, a comparison module, a target mixing genotype determination module and a mutation site screening module, wherein the different SNP loci between maternal and fetal genomic information and a reference genome can be obtained through the detection module and the comparison module, and the mixing genotype of the pseudotetraploid comprising the pregnant woman and the fetus is typed by using the target mixing genotype determination module to obtain the maternal and fetal genotypes at each SNP locus, such that all possible genetic mutations of the fetus can be detected by using only the peripheral blood sample of thepregnant woman; and with the apparatus, the separate sequencing of the sample from the father source or the mother source is not required, and all possible genetic mutations of the fetus can be detected by only using the peripheral blood sample sequencing data of the pregnant woman, such that the convenient and diversified services are provided for the fetal gene detection.

The invention discloses a method for identifying pig backfat thickness based on rs80995809 locus genotyping and its application. The method is used to detect whether the genotype of the rs80995809 locus in a pig individual is a GG genotype, a TT genotype or a GT genotype, according to the genotype of individual pigs, the backfat thickness of pigs is determined: the backfat thickness of the pigs with GG genotype is thicker than the backfat thickness of the pigs with GT genotype, and the backfat thickness of the pigs with GT genotype is greater than the backfat thickness of the pigs with TT genotype. The experiment proves that the rs80995809 locus has a significant effect on the pig backfat thickness, the selected SNP can be used as a molecular genetic marker, the numbers of trait loci thatare related or closely linked to the backfat thickness can be looked for, which can directly perform genotype selection or marker-assisted selection for large white pigs, and the breeding of dominantvarieties of the large white pigs can be accelerated.

The invention discloses a method for detecting the productive capacity of a goat. The method is to detect the 757 bit nucleic acid of a GnRHR gene in the gene group of the detected goat to be G or be mutated into A and confirms the genotype of the goat, then confirms the productive capacity of the goat by the genotype; if the 757 bit nucleic acid of the GnRHR gene in the gene group of the detected goat is G, the genotype of the homozygote thereof is AA; when the 757 bit nucleic acid of the GnRHR gene in the gene group of the detected goat is A, the genotype of the homozygote thereof is BB; the genotype of the heterozygous thereof is AB; the productive capacity of the goat of BB genotype is better than that of the goats with AB genotype and AA genotype. The method of the invention utilizes inheritance to mark the polymorphism for confirming the productive capacity of the goat, can fast and conveniently confirm the productive capacity of the goat of Jining and provides an accurate and simple method for detecting the productive capacity of the goat for the breeding of the goat.

The invention discloses a method and a special product for the assisted identification of swine backfat thickness character. The method for the assisted identification of swine backfat thickness character comprises the following steps of: determining whether the deoxyribonucleotide 47033 of the GenBank Accession Number GU565976.1 of a peroxisome proliferator-activated receptor delta gene of a to be detected swine is T, or C, or T and C, so as to determine whether the genotype of the to be detected swine is TT or TC or CC, and determining the backfat thickness character according to the genotype of the to be detected swine, wherein the backfat thickness of the to-be-detected swine with the TT genotype is greater than that of the to be detected swine with the TC genotype, and the backfat thickness of the to be detected swine with the TC genotype is greater than that of the to be detected swine with the CC genotype. The method and the special product disclosed by the invention are used for breeding swine, so that the to be detected swine can be screened in the early stage, the problem of long time needed for selecting good breeding swine in the practical production can be effectively solved, the breeding cost is reduced, and the swine backfat thickness in the practical production is effectively reduced or increased.

The invention belongs to the technical field of molecular biological breeding, and relates to a PCR marker related to cabbage dominant nuclear gene male sterilityand application thereof. A method foridentifying or assisting in identifying the cabbage dominant nuclear gene male sterility is provided, it is detected that the genotype of to-be-detected cabbage C09 chromosome 29, 141, 514 positions is A / A or A / - or - / -, and the fertility of cabbage is determined according to the genotype of to-be-detected cabbage; the to-be-detected cabbage of the A / A genotype is normal; the to-be-detected cabbage of the A / - genotype is the heterozygous sterile; to-be-detected cabbage of the A / - genotype is homozygous sterile. By means of the PCR marker and a primer, the male sterility of the cabbage is assisted to be identified; the PCR marker has the advantages of being easy to operate, high in specificity and good in stability; the coincidence rate between identification results and field phenotypes reaches100%, early breeding of cabbage varieties can be achieved, and the PCR marker has great application prospects.

The present invention is directed to methods for determining the presence or absence of a genetic defect in an IVF embryo prior to transfer comprising identifying a set of informative SNPs in the genotype of the embryo's parents; assaying the genotype of two or more informative SNPs from the set of informative SNPs on one or more chromosomes collected from a cell of the embryo; determining the presence or absence of a genetic defect in the embryo based on the genotype of the two or more informative SNPs on one or more chromosomes of the embryo; and selecting a candidate IVF embryo determined to be without genetic defect for transfer.

The invention discloses a method and a kit for detecting Chinese Holstein milk protein rate traits. The method to detect the milk protein rate of Chinese Holstein cows is to detect whether the deoxyribonucleotide of Chinese Holstein cattle from GenBank AccessionNo. , determine the genotype of the Chinese Holstein cattle, and then determine the milk protein rate trait by genotype. The kit of the present invention includes primers for PCR amplification of a genomic fragment containing nucleotides from the 873rd position from the 5' end (ie, the 279th position of GHR exon 8) from GenBank Accession No. NM_176608, restriction nucleic acid Endonuclease SSpI, PCR reagents other than the primers and restriction endonuclease buffer. The method and the kit of the invention are easy to operate, fast and sensitive, and the results detected by the method and the kit are reliable, stable and accurate. Therefore, the method and the kit of the present invention will have broad application prospects in the field of animal breeding.

It is intended to provide a means of diagnosing myocardial infarction which shows a high accuracy and a high predictability. The risk of myocardial infarction is diagnosed by a method comprising the following steps: (i) the step of analyzing 2 or more polymorphisms among 10 gene polymorphisms or 5 gene polymorphisms proved as relating to myocardial infarction; (ii) the step of determining the genotype of a nucleic acid sample based on the polymorphism data obtained in the above step; and (iii) the step of determining the genetic risk of myocardial infarction from the genotype thus obtained.

The invention discloses a method for identifying or assisting in identifying the eye muscle area of the 100-kg weight of a pig and a kit special for the method. The method includes the steps of detecting whether the 16th-site deoxyribonucleotide of the H19 gene of the pig to be detected is C or T or C and T, determining whether the gene type of the pig to be detected is TT or CC or TC, and determining the eye muscle area of the 100-kg weight of the pig according to the gene type of the pig, wherein the eye muscle area of the 100-kg weight of the pig of the TT gene type is larger than that of the pig of the CT gene type, and the eye muscle area of the 100-kg weight of the pig of the CT gene type is larger than that of the pig of the CC gene type. It is proved through tests that by means of the method, the eye muscle area of the 100-kg weight of the pig can be accurately and rapidly identified, and important reference significance in genetic breeding and sexual ability testing of the pig is achieved.

The invention discloses a molecular marker TaSnRK2.3B related with the thousand seed weight of wheat and the content of soluble sugar in wheat straw and an application of the molecular marker TaSnRK2.3B and provides a method for identification or assistant identification of to-be-detected wheat characters. The method comprises steps as follows: the genotype of the E locus and the F locus of a to-be-detected wheat population genome is detected; the to-be-detected wheat character is determined according to the genotype of the E locus and the F locus as follows: the thousand seed weight of the wheat population with the genotype of the E locus being C / C and the genotype of the F locus being G / G is higher than that of the wheat population with the genotype of the E locus being T / T and the genotype of the F locus being C / C. Experiments prove that wheat with higher thousand seed weight and smaller plant height can be found by detecting the SNP (single nucleotide polymorphism) of the two loci. The molecular marker provides a new method for molecular marker assisted selection type breeding of wheat and has great significance in culture or studies of high-yield and low-stalk wheat varieties.

The invention discloses a method for predicating the depression sheep fecundity by Fec genes. According to the method, the 746th site nucleotide of the Fec genes in the depression sheep genome to be detected is detected, so the genotype of depression sheep is determined, and then, the depression sheep fecundity is determined through the genotype; when the 746th site nucleotide of the Fec genes in the depression sheep genome is A, the homozygote genotype is ++, and when the 746th site nucleotide of the Fec genes in the depression sheep genome is G, the homozygote genotype is BB; the heterozygote genotype is B+; the fecundity of the BB genotype is higher that of the B+ genotype, and the fecundity of the B+ genotype is higher than that of ++ type. The method has the advantages that the depression sheep fecundity is determined through polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and PCR-restricted fragment length polymorphism (RFLP) genetic marker polymorphism, the depression sheep fecundity can be fast, simply and conveniently detected, and a method for accurately, simply and conveniently detecting the depression sheep fecundity is provided for the depression sheep breeding.

The invention discloses a molecular marker primer pair capable of predicting and identifying the natural length of sheep and wool and application thereof, wherein the pair of primers is shown in sequence lists Seq ID No. 1 and Seq ID No. 2. The primer pairs were designed and the sheep genomic DNA was extracted for amplification, and then digested with enzyme to obtain digested products. Enzyme digestion product electrophoresis separation, according to the electrophoresis separation result carries on the genotype determination; The results showed that the natural wool length of GG genotype population was significantly higher than that of AA genotype and GA genotype population. Finally, the ewes with GG genotype were selected as breeding population. The invention can predict and identify thenatural length of sheep wool, provides a more effective molecular marker method for improving the quality traits of sheep wool, and is applied to the field of screening sheep molecular assisted breeding with long natural length of wool fiber. The invention is simple in operation and low in cost.

The invention discloses a MUC4 molecular marker capable of identifying the susceptibility / resistibility to diarrhea of weaned piglet F4 of the western pigs and applicable to the genetic improvement of boars, and the application of the MUC4 molecular marker in breeding anti-diarrhea disease traits of the improved boars. Firstly, an identification of MUC4 polymorphic sites is processed, wherein, g.13383C is greater than T, g.15580A is greater than G, g.21208A is greater than G, and g.26002A is greater than C. Secondly, genotyping determination is applied to each of the polymorphic sites by using the PCR-RELP method. Finally, a haplotype is constructed. The invention detects the four polymorphic sites by the modern molecular biology technique, constructs the haplotype, and selects a favorable haplotype to reserve seeds for individuals by using marker assisted selection (MAS), thus being capable of significantly improving the ability to resist the diarrhea disease of the species weaned piglets and greatly reducing death rate of the weaned piglets.

The invention relates to a kit for specifically detecting the genotype of ApoE4 and application of the kit. The kit comprises sucrose water of 4 percent, a NaOH solution of 10mmol / L, a Tris-HCL (pH 7.5) solution of 1mol / L, DMSO (Dimethylsulfoxide), a reagent I, a reagent II, Hha I incision enzyme and 10*M Buffer. The application of the kit comprises the following steps of: preparing and preserving a sample; extracting genome DNA; carrying out PCR (Polymerase Chain Reaction) amplification; and identifying the genotype of ApoE4. Comparing with the prior art, the reagent provided by the kit can noninvasively obtain individual genome DNA; the ApoE4 gene can be efficiently amplified, the genotype of the ApoE4 can be quickly and accurately determined, and the genotype determination of a large quantity of individuals can be simultaneously carried out; and the kit has the advantages of short determination time and high accuracy and is suitable for large-scale screening of molecular epidemiology and preventive medicine.

Disclosed is a method for determining a genotype of a gene associated with alcohol susceptibility, which comprises the steps of: bringing a solid test sample containing the gene into direct contact with a solution comprising a buffer and a DNA polymerase and primer DNA for the amplification of the gene to carry out a method selected from a polymerase chain reaction, an LAMP method, a chain substitution amplification method, a reverse transcriptase chain substitution amplification method, a reverse transcriptase polymerase chain reaction, a reverse transcription LAMP method, an amplification method based on a nucleotide sequence, a transcription-mediated amplification method, and a rolling circle-type amplification method, thereby amplifying the gene; and determining the genotype of the gene using the amplified gene. The method enables the determination of the genotype of a gene associated with alcohol susceptibility safely, rapidly and at low cost.

The invention specifically relates to an assisted selection marker for the abdominal fat weight and carcass weight of a goose bred for the liver, belonging to the technical field of poultry breeding.The assisted selection marker has a SNP site which is located at the downstream position 807 bp of the initiator codon of the MC5R gene. The invention also discloses a method for assisted selection ofthe abdominal fat weight and carcass weight characters of the goose bred for the live by using the molecular marker. The method comprises the following steps: selecting to-be-detected Landes goose individuals; drawing blood and extracting DNAs; performing a polymerase chain reaction (PCR) by using a provided kit and detecting a product; delivering the PCR product to a sequencing company for sequencing and genotype determination; and selecting qualified individuals according to the genotypes and keeping the selected qualified individuals as breeding geese. The marker and the method of the invention can overcome the disadvantages that a slaughtering determination method leads to high cost and great workload and excellent individuals cannot be kept as breeding geese, and can be used for large-scale breeding geese selection and speed up breeding process.

The invention discloses a method for improving fecundity of Sunit sheep by gene GDF9 (growth differentiation factor 9). The genotype of Sunit sheep is determined by detecting nucleotide at the site 118 upstream of a promoter of the gene GDF9 in a genome of the to-be-tested Sunit sheep, and then fecundity of the Sunit sheep is determined according to the genotype; if the nucleotide at the site 118upstream of the promoter of a coding region of the gene GDF9 in the genome of the Sunit sheep is T, the genotype of homozygote is TT; when the 118th nucleotide upstream of the promoter of the gene coding region in the genome of the Sunit sheep is G, the genotype of homozygote is GG and the genotype of heterozygote is TG. Experiments prove that the average lambing number of the GG genotype Sunit sheep is higher than that of the GT genotype, and the average lambing number of the genotype GT Sunit sheep is higher than that of the TT genotype. Molecular marker polymorphism is detected by PCR-RFLP(restriction fragment length polymorphism) rapidly and simply, and an accurate and simple method is provided for improving fecundity of the Sunit sheep with the molecular marker assisted breeding technology.

The invention discloses a molecular marking method for predication and identification of a sheep multiple ovulation trait and molecular marker primers, and discloses the molecular marking method. With utilization of a sheep FSHR gene 5' control region c.-365C>T mutation (C-365T) mutation site, the molecular marker primers are designed to be FSHRPF and FSHRPR. The method comprises the steps: firstly, amplifying a sheep FSHR gene 5' control region section by the primers; secondly, carrying out enzyme digestion detection on the C-365T mutation site in the PCR amplified fragment by using Sau3AI; thirdly, carrying out genotype determination; and fourthly, constructing a linear model, and carrying out correlation analysis, wherein a BB genotype donor has higher ovulation number in a process of multiple ovulation, and has higher multiple ovulation efficiency. The method is simple to operate, low in detection cost, high in precision, and suitable for automatic detection. The relevance of the genotype information of the FSHR gene 5' control region section with the sheep multiple ovulation trait is utilized.

For automatic genotype determination based on microsatellites, when a +A peak occurrence pattern largely varies due to excessively intense fluorescent signal, incapability of separating peaks completely, and incomplete experimental conditions, a decreased precision in noise removal and time requirement for visual check and correction become bottlenecks in the analysis. On the basis of data about alleles which have been reported to be observed in a marker, it is investigated whether or not a sample is suitable to be used for examining a +A peak occurrence pattern, which peak can be a starting point for examining the +A peak occurrence pattern, and whether the reported allele is a true peak or the +A peak thereof. According to the obtained data, the +A peak occurrence pattern is estimated. In this process, it is possible to perform an accurate estimation, and therefore, a precision for noise removal in automatic genotype discrimination can be improved.

The invention provides a primer for identifying recessive white feather genotype of Xianglu mountain chickens and an application of the primer. Two pairs of primers, i.e., three primers, are adopted through design; double PCR amplification are carried out on the TYR gene colored feather allele C and the white feather allele c, the amplified PCR product is electrophoresed on 1.2% agarose gel electrophoresis, and then the agarose gel is photographed by a gel-imaging system, and the obtained electrophoresis pattern result is subjected to genotype determination: if only a 169 bp band can be obtained, the allelotype of the chicken to be tested is cc-type recessive white feather homozygotic type; if only a 526 bp band can be obtained, the allelotype genotype of the test chicken is CC colored feather homozygotic type; and if both bands of 169 bp and 526 bp are obtained at the same time, the allelotype of the chicken to be tested is a Cc colored heterozygous type. The primer disclosed by the invention has the characteristic of rapidly and accurately identifying colored feather homozygotic type and recessive homozygotic type and heterozygosis individuals of the Xianglu mountain chickens.