Method for detecting depression sheep fecundity

Abstract

The invention discloses a method for predicating the depression sheep fecundity by Fec genes. According to the method, the 746th site nucleotide of the Fec genes in the depression sheep genome to be detected is detected, so the genotype of depression sheep is determined, and then, the depression sheep fecundity is determined through the genotype; when the 746th site nucleotide of the Fec genes in the depression sheep genome is A, the homozygote genotype is ++, and when the 746th site nucleotide of the Fec genes in the depression sheep genome is G, the homozygote genotype is BB; the heterozygote genotype is B+; the fecundity of the BB genotype is higher that of the B+ genotype, and the fecundity of the B+ genotype is higher than that of ++ type. The method has the advantages that the depression sheep fecundity is determined through polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and PCR-restricted fragment length polymorphism (RFLP) genetic marker polymorphism, the depression sheep fecundity can be fast, simply and conveniently detected, and a method for accurately, simply and conveniently detecting the depression sheep fecundity is provided for the depression sheep breeding.

Description

technical field [0001] A method for testing the fecundity of lowland sheep. Background technique [0002] Fec B The gene was discovered in Australia's Booroola Merino sheep breed in 1985 and is the first major gene described to affect sheep ovulation rate and fecundity. Booroola mutations, point mutations in the coding region of the BMPR-IB locus, have been crossed into other sheep breeds, and other productive sheep breeds other than Booroola Merino sheep have also been found in the natural state. Fec B The gene is located in the narrow region of sheep chromosome 6 6q23~q31, between osteopontin and epidermal growth factor. Fec B Genotype assignment of loci, initially based on detection of ovulation rates and lambing records, later used to link genetic markers to the Booroola gene, can now be obtained by molecular genotyping of individuals at the BMPR-IB locus. a Fec B One copy increases the number of ovulations by 1.3-1.6, two copies of FecB increase the number of ovul...

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Problems solved by technology

In particular, the local coastal wetlands are severely saline-alkali, which is very corrosive to sheep's hoofs, and it is difficult for other breeds of sheep to adapt

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