Single nucleotide polymorphism (SNP) of ADD1 gene of cattle and detection method thereof

A single nucleotide polymorphism and detection method technology, which is applied in the application field of cattle breeding, can solve problems such as inconsistent research results, and achieve the effects of easy popularization and application, high accuracy and low cost

Inactive Publication Date: 2011-04-27
XUZHOU NORMAL UNIVERSITY
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Problems solved by technology

However, the research results on the relationship between ADD1 gene variation and hypertension are quite inconsistent.
The research on ADD1 gene and growth and d...

Method used

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  • Single nucleotide polymorphism (SNP) of ADD1 gene of cattle and detection method thereof
  • Single nucleotide polymorphism (SNP) of ADD1 gene of cattle and detection method thereof
  • Single nucleotide polymorphism (SNP) of ADD1 gene of cattle and detection method thereof

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Embodiment Construction

[0029] The present invention first designs primers according to the conservative sequence of the ADD1 gene, uses the genomic DNA of three cattle breeds as templates to perform PCR amplification, mixes the PCR products, purifies them, and sequence them. Then, perform sequencing map analysis and sequence comparison to screen out SNP sites; secondly, perform PCR-SSCP detection of polymorphic sites in the population to be tested; finally, perform population genetic statistical analysis based on the genotypes detected in the population Based on correlation analysis with growth traits, molecular markers closely related to cattle growth traits were screened out. The present invention will be described in detail below, which is an explanation rather than a limitation of the present invention.

[0030] a. Cloning of partial DNA sequence of ADD1 gene of local cattle breed

[0031] 1. Collection and processing of blood samples

[0032] Take a blood sample of 10mL, add 500μL of 0.5mol / L EDTA f...

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Abstract

The invention discloses a method for detecting the single nucleotide polymorphism (SNP) of an ADD1 gene of cattle, which comprises the following steps: through taking a genomic DNA of cattle containing an ADD1 gene as a template and taking a primer mix P as a primer, the ADD1 gene of cattle is amplified through PCR (polymerase chain reaction), then the segment size of the obtained product is amplified through AGE (agarose gel electrophoresis) detection; and then, the detection on SSCP (single strand conformation polymorphism) is performed. The polymorphism of the gene comprises a base polymorphism that the 70027th bit in an ADD1 gene sequence table SEQID No.8 of cattle is G or A, and a base polymorphism that the 70051th bit in the ADD1 gene sequence table SEQID No.8 of cattle is G or A. The analysis on the correlation between the SSCP polymorphism and the production performance shows that: a certain correlation exits between the polymorphic site and the production performance, therefore, the weight of a Qinchuan cattle (genotype: BB) is significantly higher than that of a cattle (genotype: AA). The method is used for the auxiliary selection and molecular breeding of cattle through screening and detecting molecular genetic markers closely relating to the growth properties of cattle on a DNA level, therefore, the method plays a vital role in improving the production performance of cattle.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and relates to the screening and detection of single nucleotide polymorphism (SNP) of the ADD1 gene of cattle and its application as an auxiliary selection marker in cattle breeding. Background technique [0002] Gene polymorphism refers to the difference in genome sequence between different species or different individuals within the same species. These differences are caused by nucleotide changes in DNA alleles in chromosomes, which include base substitutions, insertions, deletions, and duplicate sequence copies Number of changes. [0003] Single nucleotide polymorphism refers to the polymorphism caused by the substitution of a single nucleotide (A / T / C / G) in the genomic DNA sequence, which is mainly caused by the conversion or transversion of a single base. SNPs with conversion variants accounted for about 2 / 3, and other SNPs were at similar levels. The cytosine of CpG dinucleotide is the most prone to ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 陈宏钱丽娜张春雷房兴堂
Owner XUZHOU NORMAL UNIVERSITY
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