48 results about "Qinchuan cattle" patented technology

The invention discloses a method for detecting the body sizes of Qinchuan cattle through using ANAPC13 gene. The method adopts the combination of a PCR-SSCR technology with DNA sequencing to detect the variation sites of the ANAPC13 gene of the cattle. An SSCP result shows that there are three banding patterns, and a sequencing result shows that there are two variation sites comprising an A-G mutation at 17bp of a first exon and a C-T mutation at 42bp of the first exon. Later sequencing verification shows that the mutations of the two sites in 98% of individuals belong to a linkage mutation. So the three banding patterns are named as AACC, AGCT and GGTT respectively, and the Qinchuan cattle with the number being 404 undergo parting statistics and association analysis of the parting statistics with the body size character. The method is used for auxiliary selection and molecular breeding of the Qinchuan cattle to accelerate the breeding speed of fine breeds of the Qinchuan cattle through screening and detecting molecular genetic markers closely related with the growth and development traits of the Qinchuan cattle in DNA level. The body sizes of the Qinchuan cattle are predicted and determined through the analytic comparison of the genotype, so the method has the advantages of simple operation, low cost, high accuracy, and rapid detection.