48 results about "Qinchuan cattle" patented technology

The invention discloses a method for detecting a CNV (Copy Number Variation) mark of a gene GBP2 of Qinchuan cattle and the application of the CNV mark. The method comprises the following steps: on the basis of a real-time fluorescent quantitation PCR (Polymerase Chain Reaction) technology, by taking the DNA of a whole genome of blood of the Qinchuan cattle as a template, respectively multiplying two CNV regions of the gene GBP2 of the cattle by using two pairs of specific primers GBP2-CNV1 and GBP2-CNV2, multiplying a gene BTF3, which serves as a contrast, of the Qinchuan cattle by using another pair of specific PCR primers R1, and judging whether the copy number of individuals is missing or not according to a calculation result of 2-delta delta Ct. According to the method provided by the invention, on the basis of the relevance between missing of the copy number of the gene GBP2 of the Qinchuan cattle and a growth character, the Qinchuan cattle molecular mark assistant selection breeding work is accelerated favorably. The method is simple, quick and convenient for popularization and application.

The invention discloses a method for detecting the body sizes of Qinchuan cattle through using ANAPC13 gene. The method adopts the combination of a PCR-SSCR technology with DNA sequencing to detect the variation sites of the ANAPC13 gene of the cattle. An SSCP result shows that there are three banding patterns, and a sequencing result shows that there are two variation sites comprising an A-G mutation at 17bp of a first exon and a C-T mutation at 42bp of the first exon. Later sequencing verification shows that the mutations of the two sites in 98% of individuals belong to a linkage mutation. So the three banding patterns are named as AACC, AGCT and GGTT respectively, and the Qinchuan cattle with the number being 404 undergo parting statistics and association analysis of the parting statistics with the body size character. The method is used for auxiliary selection and molecular breeding of the Qinchuan cattle to accelerate the breeding speed of fine breeds of the Qinchuan cattle through screening and detecting molecular genetic markers closely related with the growth and development traits of the Qinchuan cattle in DNA level. The body sizes of the Qinchuan cattle are predicted and determined through the analytic comparison of the genotype, so the method has the advantages of simple operation, low cost, high accuracy, and rapid detection.