A method for detecting the cnv marker of Qinchuan cattle gbp2 gene and its application

Abstract

The invention discloses a method for detecting a CNV (Copy Number Variation) mark of a gene GBP2 of Qinchuan cattle and the application of the CNV mark. The method comprises the following steps: on the basis of a real-time fluorescent quantitation PCR (Polymerase Chain Reaction) technology, by taking the DNA of a whole genome of blood of the Qinchuan cattle as a template, respectively multiplying two CNV regions of the gene GBP2 of the cattle by using two pairs of specific primers GBP2-CNV1 and GBP2-CNV2, multiplying a gene BTF3, which serves as a contrast, of the Qinchuan cattle by using another pair of specific PCR primers R1, and judging whether the copy number of individuals is missing or not according to a calculation result of 2-delta delta Ct. According to the method provided by the invention, on the basis of the relevance between missing of the copy number of the gene GBP2 of the Qinchuan cattle and a growth character, the Qinchuan cattle molecular mark assistant selection breeding work is accelerated favorably. The method is simple, quick and convenient for popularization and application.

Description

Technical field [0001] The present invention belongs to the field of molecular genetics research, and specifically relates to a method for detecting copy number variation of GBP2 gene of Qinchuan cattle. The method uses real-time quantitative PCR of genomic DNA and takes BTF3 gene as a reference. According to 2 -ΔΔCt Value to determine the individual's copy number variation. Background technique [0002] With the rapid development of related disciplines such as genomics and bioinformatics, the theory and technology of animal genetic breeding have also undergone major changes. Beef cattle breeding has also shifted from traditional conventional phenotypic breeding to molecular breeding. At present, molecular breeding His research mainly focuses on molecular mark-assist selection (MAS), which uses DNA molecular markers to select genetic resources or breeding materials, and to improve the comprehensive traits of livestock and poultry. In livestock and poultry breeding, through the se...

AI Technical Summary

Problems solved by technology

Some studies have been done on the function of the GBP2 gene in humans and mice, and found that it has an important impact on the regulation of cell growth and disease resistance, but there are few reports on cattle

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