BBS2 molecular marker associated with beef quality traits of beef cattle, and detection kit thereof
A technology of meat quality traits and molecular markers, which is applied in the determination/testing of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., and can solve the problems of unreported correlation analysis of meat quality traits
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[0056] Embodiment 1: test material and method
[0057] 1. Test material
[0058] 1.1 Test animals
[0059] Taking the Qinchuan cattle group, which is representative of local yellow cattle in my country, as the test object, blood samples were collected through venous blood collection.
[0060] 1.2 Test reagents
[0061] Agarose (BIOWEST); PCR primers were synthesized by Sangon Bioengineering (Shanghai) Co., Ltd.; blood genome extraction kit.
[0062] 1.3 DNA analysis software
[0063] PCR primer design software: Primer Premier 5
[0064] Sequence analysis software: DNAman, Chromas
[0065] Data analysis software: IBM SPSS statistics 19
[0066] 2. Detection of genetic polymorphism
[0067] The extraction of genomic DNA was performed according to the instructions of the blood genomic DNA extraction kit from TIANGEN Company, the quality of genomic DNA was detected by 1.2% agarose gel electrophoresis, and the extracted genomic DNA was stored at -80°C for later use.
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