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BBS2 molecular marker associated with beef quality traits of beef cattle, and detection kit thereof

A technology of meat quality traits and molecular markers, which is applied in the determination/testing of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc., and can solve the problems of unreported correlation analysis of meat quality traits

Active Publication Date: 2021-07-30
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the research on the SNPs of this gene and its correlation with beef cattle growth and meat quality traits

Method used

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  • BBS2 molecular marker associated with beef quality traits of beef cattle, and detection kit thereof
  • BBS2 molecular marker associated with beef quality traits of beef cattle, and detection kit thereof
  • BBS2 molecular marker associated with beef quality traits of beef cattle, and detection kit thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Embodiment 1: test material and method

[0057] 1. Test material

[0058] 1.1 Test animals

[0059] Taking the Qinchuan cattle group, which is representative of local yellow cattle in my country, as the test object, blood samples were collected through venous blood collection.

[0060] 1.2 Test reagents

[0061] Agarose (BIOWEST); PCR primers were synthesized by Sangon Bioengineering (Shanghai) Co., Ltd.; blood genome extraction kit.

[0062] 1.3 DNA analysis software

[0063] PCR primer design software: Primer Premier 5

[0064] Sequence analysis software: DNAman, Chromas

[0065] Data analysis software: IBM SPSS statistics 19

[0066] 2. Detection of genetic polymorphism

[0067] The extraction of genomic DNA was performed according to the instructions of the blood genomic DNA extraction kit from TIANGEN Company, the quality of genomic DNA was detected by 1.2% agarose gel electrophoresis, and the extracted genomic DNA was stored at -80°C for later use.

[0068...

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PUM

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Abstract

The invention discloses a BBS2 molecular marker associated with beef quality traits of beef cattle, a detection kit and application of the BBS2 molecular marker. The molecular marker is located at a g.24223562G / A site, a g.24226239C / T site and a g.24227851G / A site of a BBS2 gene coding region, and the molecular marker is remarkably related to beef cattle eye muscle area, eye muscle depth and intermuscular fat content meat quality traits. The marker is a potential breeding genetic marker for local yellow cattle meat represented by Qinchuan cattle. Based on the discovery, the kit for rapidly detecting the BBS2 genotype to predict the beef quality traits of the beef cattle is developed, and the kit can be used for early breeding of local cattle represented by Qinchuan cattle, the genetic progress of the beef cattle is accelerated, and the production cost is saved. The beef cattle early-stage selection and reservation are realized, and the beef breeding process is accelerated.

Description

technical field [0001] The invention belongs to the technical field of animal molecular markers, and in particular relates to a BBS2 molecular marker related to beef quality traits, a detection kit and applications thereof. Background technique [0002] Improved breeds of livestock and poultry are the "chips" of animal husbandry, contributing more than 40% to the development of animal husbandry. There are 55 local cattle breeds in my country, which is one of the countries with the most abundant cattle breed resources in the world. However, due to the influence of the long-term use mainly for draft and the late start of meat breeding, compared with foreign specialized beef cattle breeds, the local yellow cattle that currently support the development of my country's beef cattle industry generally have slower growth rates and less carcass meat production. , low beef quality and many other deficiencies. Although some results have been achieved in improving local cattle breeds t...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 成功张孜怡杨森陈嘉玥田媛龙凤李奇隆昝林森
Owner NORTHWEST A & F UNIV
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