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Method for detecting GAL3ST1 gene copy number variation of cattle and application thereof

A technology of gene copy number and copy number variation, applied in the field of molecular genetics detection, can solve problems such as cattle breeds that have not yet been seen, and achieve the effect of convenient marker-assisted selection and low cost

Active Publication Date: 2020-07-10
NORTHWEST A & F UNIV
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Problems solved by technology

At present, there is no literature report on the effect of GAL3ST1 gene CNVs on the growth traits of cattle breeds (for example, Qinchuan cattle)

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  • Method for detecting GAL3ST1 gene copy number variation of cattle and application thereof
  • Method for detecting GAL3ST1 gene copy number variation of cattle and application thereof
  • Method for detecting GAL3ST1 gene copy number variation of cattle and application thereof

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Embodiment Construction

[0027] The invention will be described in further detail below in conjunction with the accompanying drawings and embodiments, and the embodiments are explanations of the present invention, rather than limiting the protection scope of the present invention.

[0028] In the previous study on cattle genome resequencing, it was found that there was a copy number variation between position 71847414 and position 71869923 (NC_007315.6) of the bovine GAL3ST1 gene sequence. The present invention designs specific fragment amplification primers according to the region of copy number variation in the GAL3ST1 gene sequence obtained by resequencing, and uses Qinchuan cattle genomic DNA as a template to perform qPCR amplification. According to the quantitative results (using the general transcription factor 3 gene, That is, the BTF3 gene is used as the internal reference gene) to calculate and determine the type of copy number variation of the individual. The association analysis between dif...

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Abstract

The invention discloses a method for detecting copy number variation of a GAL3ST1 gene of cattle and application thereof. According to the method, based on real-time quantitative PCR, the DNA of a genome of cattle (such as Qinchuan cattle) is used as a template, partial fragments of the copy number variation region of the GAL3ST1 gene of cattle are amplified by utilizing a pair of specific PCR primers, partial fragments of a universal transcription factor 3 gene of the cattle are used as internal reference by utilizing another pair of specific PCR primers, the quantitative result is calculatedby utilizing a method of 2*2-delta delta Ct, and the copy number variation type of an individual is determined. The method is established on the correlation basis between the copy number variation and growth traits of the GAL3ST1 gene of cattle and is favorable to accelerating the cattle molecular mark assisted selective breeding work. The method is simple and quick and is convenient for popularization and application.

Description

technical field [0001] The invention belongs to the field of molecular genetics detection, and in particular relates to a method for detecting the variation of the copy number of cattle GAL3ST1 gene. -ΔΔCt The calculated value thus determines the copy number variation type of the individual. Background technique [0002] With the rapid development of biotechnology, the study of copy number variation has entered a period of vigorous development. The so-called copy number variation (Copy Number Variations, CNVs) refers to the insertion or deletion variation of the genome sequence greater than 50 bp between two individuals, which belongs to the type of genome structure variation. CNV can affect the function of genes and the phenotype of individuals through dose effects, position effects, blocking functional genes, fusion genes, exposure of recessive alleles and potential transition effects. With the completion of the whole genome sequencing of cattle, the study of CNVs in bov...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/156C12Q2600/124
Inventor 黄永震梁君桐蔡翠翠彭梦阳贺花亐开兴张子敬施巧婷黄必志雷初朝陈宏
Owner NORTHWEST A & F UNIV
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