Method for detecting body sizes of Qinchuan cattle through using ANAPC13 gene

Abstract

The invention discloses a method for detecting the body sizes of Qinchuan cattle through using ANAPC13 gene. The method adopts the combination of a PCR-SSCR technology with DNA sequencing to detect the variation sites of the ANAPC13 gene of the cattle. An SSCP result shows that there are three banding patterns, and a sequencing result shows that there are two variation sites comprising an A-G mutation at 17bp of a first exon and a C-T mutation at 42bp of the first exon. Later sequencing verification shows that the mutations of the two sites in 98% of individuals belong to a linkage mutation. So the three banding patterns are named as AACC, AGCT and GGTT respectively, and the Qinchuan cattle with the number being 404 undergo parting statistics and association analysis of the parting statistics with the body size character. The method is used for auxiliary selection and molecular breeding of the Qinchuan cattle to accelerate the breeding speed of fine breeds of the Qinchuan cattle through screening and detecting molecular genetic markers closely related with the growth and development traits of the Qinchuan cattle in DNA level. The body sizes of the Qinchuan cattle are predicted and determined through the analytic comparison of the genotype, so the method has the advantages of simple operation, low cost, high accuracy, and rapid detection.

Description

technical field [0001] The invention belongs to the field of zoology, and in particular relates to a method for predicting and detecting the body size of Qinchuan cattle by using genes. The method can detect the body length, body height, waist height, waist angle width, bust circumference and ischial end width. Background technique [0002] Qinchuan cattle have a long history. In the eighth century BC, there was a record of "choosing good cattle to offer to the master" in Guanzhong. The working people have always used big cattle as seeds, requiring the bulls to be one long (long trunk), two squares (mouth square, buttocks square), three wide (forehead wide, chest wide, hindquarters wide), and four tight (four hooves forked). Tight), five short (short neck, short limbs), non-purplish red does not work as a seed. As early as 1950, Xu Zhenying, an academician of the Chinese Academy of Sciences and a professor of the Northeast Agricultural College, pointed out: "The Qinchuan c...

AI Technical Summary

Problems solved by technology

Some studies have shown that the variation of this gene significantly affects the height of humans. At present, there are few studies on the variation of this gene, and there is no relevant report on cattle

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