Detection method for 19-bp duplication and deletion polymorphism of Chinese yellow cattle PLAG1 (Pleomorphic Adenoma Gene 1) gene and application of detection method

A PLAG1, 19-bp technology, which is applied in the determination/testing of microorganisms, biochemical equipment and methods, recombinant DNA technology, etc., can solve the problems of low mutation frequency, long reproduction and breeding cycle, genetic resource limitation, etc., and achieve low cost effect

Inactive Publication Date: 2018-11-23
NORTHWEST A & F UNIV
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Problems solved by technology

Therefore, traditional breeding techniques face many challenges such as low mutati

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  • Detection method for 19-bp duplication and deletion polymorphism of Chinese yellow cattle PLAG1 (Pleomorphic Adenoma Gene 1) gene and application of detection method
  • Detection method for 19-bp duplication and deletion polymorphism of Chinese yellow cattle PLAG1 (Pleomorphic Adenoma Gene 1) gene and application of detection method
  • Detection method for 19-bp duplication and deletion polymorphism of Chinese yellow cattle PLAG1 (Pleomorphic Adenoma Gene 1) gene and application of detection method

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[0030] This embodiment provides a kind of using the whole genome DNA of Chinese yellow cattle as a template, using primer pair P1 to amplify a partial fragment of PLAG1 gene of Chinese yellow cattle by PCR; then carry out agarose gel electrophoresis to the fragments obtained by PCR amplification; identify according to the electrophoresis results The genotype of the insertion / deletion polymorphic site on the PLAG1 gene of Chinese yellow cattle;

[0031] The primer pair P1 is:

[0032] Upstream primer: 5'-GATCCCACCTGACAAACG-3';

[0033] Downstream primer: 5'-CTAGAGCTGCCTCGCACA-3'.

[0034] Wherein, the insertion / deletion polymorphic site on the Chinese cattle PLAG1 gene refers to the 19-bp repeat deletion polymorphic fragment GAACACACAGCCCACAAGG present on the AC_000171.1g.3747444-3747462 site of the Chinese cattle PLAG1 gene.

[0035] In this example, the reaction program of PCR amplification is: pre-denaturation at 95.0°C for 5 minutes; denaturation at 95.0°C for 30s, annealin...

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Abstract

The invention discloses a detection method for 19-bp duplication and deletion polymorphism of a Chinese yellow cattle PLAG1 (Pleomorphic Adenoma Gene 1) gene and application of the detection method. The method takes Chinese yellow cattle whole genome DNA (Deoxyribonucleic Acid) as a template, and a primer pair P1 is used for treating a partial segment of the Chinese yellow cattle PLAG1 gene through PCR (Polymerase Chain Reaction) amplification; then the segment obtained by the PCR amplification is subjected to agarose gel electrophoresis; a gene type of an insertion/deletion polymorphism siteon the Chinese yellow cattle PLAG1 gene is identified according to an electrophoresis result; different gene types of the duplication and deletion polymorphism of the gene are remarkably related withgrowth properties including body height, body length, chest circumference, chest width, chest depth, waist height and the like four types of Chinese yellow cattle including Qiachuan cattle, Nanyang cattle, Jiaxian cattle and Luxi cattle. The method disclosed by the invention can be applied to marker-assisted selection breeding of growth characters of beef cattle, and a good genetic resource groupwith good growth performance is easily and rapidly established.

Description

technical field [0001] The invention belongs to the field of biotechnology and livestock breeding, and relates to the detection of gene insertion and deletion (inde), in particular to a detection method and application of the 19-bp repeat deletion polymorphism of cattle PLAG1 gene, which can be quickly and accurately detected The 19-bp repeat deletion polymorphism of PLAG1 gene AC_000171.1 in Chinese yellow cattle. Background technique [0002] In order to optimize production and meet the growing human demand for animal products, the direction of livestock breeding and breeding techniques have undergone a series of historic changes. We usually use traditional breeding such as hybrid improvement and strain selection to continuously improve beef cattle breeds, and its efficiency depends on the identification of high-value individuals, selection intensity, reproduction and breeding cycles, and limited genetic diversity. Therefore, traditional breeding techniques face many chal...

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 王淑辉孙秀柱李泽樊路杰王国艳江钧益
Owner NORTHWEST A & F UNIV
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