Method for detecting Qinchuan cattle Wnt7a gene single nucleotide polymorphism, and application thereof

A single nucleotide polymorphism, Qinchuan cattle technology, applied in the field of molecular genetics, can solve problems such as affecting the expression level of protein structure and lack of research.

Inactive Publication Date: 2013-09-25
NORTHWEST A & F UNIV
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Problems solved by technology

[0007] (2) Representative: Some SNPs located inside the gene may directly affect the protein structure or expression level
The research on Wnt7a gene polymorphism is al

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  • Method for detecting Qinchuan cattle Wnt7a gene single nucleotide polymorphism, and application thereof
  • Method for detecting Qinchuan cattle Wnt7a gene single nucleotide polymorphism, and application thereof
  • Method for detecting Qinchuan cattle Wnt7a gene single nucleotide polymorphism, and application thereof

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Embodiment Construction

[0033] The present invention detects the single nucleotide polymorphism that may cause a change in the conformation of the encoded protein due to the missense mutation at position 63777 of the Wnt7a gene of Qinchuan cattle, so as to be used for marker-assisted selection of growth traits of Chinese Qinchuan beef, and quickly Establish a Qinchuan cattle population with excellent genetic resources. In the following, the present invention will be further described in detail in combination with the detection of specific samples and the association of traits, which is an explanation of the present invention rather than a limitation.

[0034] a. Design of PCR primers for the fourth exon region of Qinchuan cattle Wnt7a gene

[0035] Taking the bovine Wnt7a gene (NC_007320.5) sequence published by NCBI as a reference, Primer 5.0 was used to design PCR primers capable of amplifying the fourth exon region of the Qinchuan cattle Wnt7a gene. The sequence of the primer M is as follows:

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Abstract

The invention discloses a method for detecting Qinchuan cattle Wnt7a gene single nucleotide polymorphism (SNP). According to the invention, genome DNA of a Chinese local cattle breed Qinchuan cattle is adopted as a template; Wnt7a gene sequence is subjected to PCR amplification by using Taq DNA polymerase, Buffer (a buffer solution), Mg<2+>, dNTPs and primers M; a PCR product is subjected to enzyme digestion by using restriction endonuclease Alw44I; a fragment is subjected to separation and detection through agarose gel electrophoresis, and Qinchuan cattle Wnt7a gene single nucleotide polymorphism is finally determined. The Wnt7a gene function is related to growth traits such as weight and body length, such that the detection method provided by the invention establishes a basis for establishing a relationship between the SNP of the Wnt7a gene and growth traits. Therefore, marker-assisted selection of meat-purpose growth traits of Chinese Qinchuan cattle is facilitated, and a Qinchuan cattle population with excellent genetic resource can be rapidly established.

Description

technical field [0001] The invention belongs to the field of molecular genetics and relates to the detection of gene single nucleotide polymorphism (SNP), in particular to a method for detecting the 63777th SNP of Qinchuan cattle Wnt7a gene. Background technique [0002] Gene polymorphism refers to polymorphism (polymorphism), which means that in a randomly mated population, more than two genotypes can exist at the same gene locus. Gene polymorphism in biological groups is very common, among which, the structure, expression and function of human genes have been studied in depth. Human gene polymorphisms come from both the difference in the copy number of repeated sequences in the genome, the variation of single-copy sequences, and the conversion or replacement of biallelic genes. According to the order of attention and research, it is usually divided into three categories: DNA fragment length polymorphism, DNA repeat sequence polymorphism, and single nucleotide polymorphism...

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 陈宏薛璟孙加节蓝贤勇雷初朝
Owner NORTHWEST A & F UNIV
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