38 results about "FOXO1" patented technology

The present invention relates to uses of glycerokinase as a glucose metabolism disorder treatment target. According to the present invention, the results show that glycerokinase can catalyze glycerol to generate 3-phosphoglycerol so as to participate in gluconeogenesis, can regulate the expressions of gluconeogenesis enzyme glucose-6-phosphatase (G6pase) and phosphoenolpyruvate carboxykinase (PEPCK) through an AKT-FOXO1 signaling pathway so as to regulate gluconeogenesis, and can provide important effects in the maintaining of the blood glucose homeostasis, such that the glycerokinase and the regulator thereof are the new target for blood glucose regulation and diabetes treatment.

The invention discloses a reagent used for detecting forkhead transcription factor O1 (FOXO1) gene mutation, a polymerase chain reaction (PCR) detection method and an application, belonging to the technical field of basic biological research and biological detection. The detection reagent comprises primers, peptide nucleic acid (PNA) fluorescent probes and wild type complementary PNA sequences, wherein the forward primers are shown in SEQ ID NO.1 and NO.3 in a sequence table; the reverse primers are shown in SEQ ID NO.2 and NO.4 in the sequence table; the PNA fluorescent probes are shown in SEQ ID NO.5 and NO.6 in the sequence table; and the wild type complementary PNA sequences are shown in SEQ ID NO.7 and NO.8 in the sequence table. The reagent can rapidly discover mutation of the FOXO1 gene in a Wnt signal pathway from the transcriptional level, has the obvious advantages of strong specificity, high sensitivity, and the like and provides a tool for anti-cancer drug screening, new targeted drug discussion and basic scientific research.

The invention discloses cattle FoxO1 gene mononucleotide polymorphism and a detection method thereof. The gene mononucleotide polymorphism comprises that: with a Qinchuan cattle whole genome DNA comprising the FoxO1 gene as a template, and with a primer pair P as primers, the Qinchuan cattle FoxO1 gene is subjected to PCR amplification; a restriction endonuclease HhaI is used for digesting the PCR amplification product, and the digested segment is subjected to agarose gel electrophoresis; and according to the electrophoresis result, base polymorphism on the 178132 site of the Qinchuan cattle FoxO1 gene is identified. The FoxO1 gene is important for animal muscle fat traits and growth traits. With the method, the molecular genetic marker closely related to Qinchuan cattle growth traits is screened and detected on a DNA level. The method is used in Qinchuan cattle assisted selection and molecular breeding, and assists in accelerating Qinchuan cattle breeding speed.