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38 results about "FOXO1" patented technology

Forkhead box protein O1 (FOXO1) also known as forkhead in rhabdomyosarcoma is a protein that in humans is encoded by the FOXO1 gene. FOXO1 is a transcription factor that plays important roles in regulation of gluconeogenesis and glycogenolysis by insulin signaling, and is also central to the decision for a preadipocyte to commit to adipogenesis. It is primarily regulated through phosphorylation on multiple residues; its transcriptional activity is dependent on its phosphorylation state.

Benzimidazole derivative, and preparation method and application thereof

The invention relates to a benzimidazole derivative 2-m-phenylaminobenzimidazolyl-1H-pyrazolyl-3-formamide of which the structural formula is disclosed in the specification. The benzimidazole derivative can promote growth of microzyme, enhance the metabolic activity of the microzyme, obviously lower the plasma triglyceride, total cholesterol and high density lipoprotein of a C57 BL / 6J mouse which is fed by high-fat feed for a long time, lower the contractive pressure and diastolic pressure of the model mouse, lower the fasting blood-glucose of the model mouse, improve the sugar tolerance of the mouse, influence the expression of SIRT1 and related genes (FOXO1, P53, PPARgamma and PGC-1alpha), and obviously improve the metabolism of glucose and fat; and therefore, the benzimidazole derivative can be used for preparing medicines for preventing and treating diabetes and complications thereof.
Owner:斯芬克司药物研发(天津)股份有限公司

Qinchuan cattle FoxO1 gene mononucleotide polymorphism molecular marker detection method and application

The invention discloses cattle FoxO1 gene mononucleotide polymorphism and a detection method thereof. The gene mononucleotide polymorphism comprises that: with a Qinchuan cattle whole genome DNA comprising the FoxO1 gene as a template, and with a primer pair P as primers, the Qinchuan cattle FoxO1 gene is subjected to PCR amplification; a restriction endonuclease HhaI is used for digesting the PCR amplification product, and the digested segment is subjected to agarose gel electrophoresis; and according to the electrophoresis result, base polymorphism on the 178132 site of the Qinchuan cattle FoxO1 gene is identified. The FoxO1 gene is important for animal muscle fat traits and growth traits. With the method, the molecular genetic marker closely related to Qinchuan cattle growth traits is screened and detected on a DNA level. The method is used in Qinchuan cattle assisted selection and molecular breeding, and assists in accelerating Qinchuan cattle breeding speed.
Owner:NORTHWEST A & F UNIV

Detection method of yak FOXO1 gene single nucleotide polymorphism and kit thereof

The invention relates to the technical field of gene polymorphism detection, in particular to a detection method of yak FOXO1 gene single nucleotide polymorphism and a kit thereof. The detection method comprises the following steps: 1) preparing a yak FOXO1 gene amplification product; 2) synthesizing a high and low temperature interior label; 3) preparing an HRM-PCR (High Resolution Melt-Polymerase Chain Reaction) amplification product; 4) collecting a fluorescence signal. Compared with an HRM method used for detecting polymorphic sites in the prior art, a gene probe designed by the detection method is more accurate and flexible in positioning, is accurate in parting, does not need PCR post-processing, has high working efficiency and good repeatability and is low in sample quality and quantity requirements, especially, the concentration requirement of a DNA template can be lowered to 0.1ng / mu L, and time and labor are saved when a sample size is large. Meanwhile, the detection kit has the advantages of high sensitiveness, high detection speed and good stability.
Owner:GANSU AGRI UNIV

Modulation of PPARgamma2 gene promoter by FOXO1

A method for detecting a modulator of transcription of a human PPAR gene promoter is provided, comprising contacting a candidate compound with a cell transfected with an expression vector containing a heterologous gene operably linked to a PPAR promoter and an additional expression vector containing the FOXO1 gene, and comparing the level of expression of said heterologous gene in the presence of the compound and in the absence thereof, whereby a modulator of transcription of the human PPAR gene promoter is identified. The PPAR gene promoter is preferably the PPARγ2 promoter, and the DNA-binding domain of the FOXO1 protein binds to a sequence encompassing the 63 to 323 bp region of the human PPARγ2 promoter, preferably the 270 to 310 bp region.
Owner:TECHNION RES & DEV FOUND LTD

Application of FoxO1 gene in drug preparation

The invention belongs to the field of biological medicine and particularly relates to an application of a FoxO1 gene in drug preparation. According to the invention, the function of the FoxO1 gene in osteoblast proliferation differentiation under high sugar condition is researched so as to research the function of the FoxO1 gene in bone injury and osteoporosis induced by diabetes, in order to provide theoretical basis and experimental data for clinical treatment of diabetic bone injury and osteoporosis disease. The invention verifies that the FoxO1 gene can promote osteoblast proliferation, FoxO1 is used as a factor used for mainly regulating and controlling bone mass and has an antioxidant stress function on osteoblast, the FoxO1 gene generates a certain protection function on osteoporosis no matter in environment with increased oxidative stress level caused by ageing or diabetes or high blood sugar, and provides a new target for research and development of new anti-osteoporosis drugs.
Owner:RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE

Molecular marker of rhabdomyosarcoma fusion gene related circular RNA and application of molecular marker

The invention provides a molecular marker of rhabdomyosarcoma fusion gene related circular RNA and application of the molecular marker. The molecular marker of the rhabdomyosarcoma PAX3-FOXO1 fusion gene related circular RNA is F-circP3F, and the nucleotide sequence of the molecular marker is as shown in SEQ ID No. 1. The F-circP3F provided by the invention has very high sensitivity and specificity to alveolar rhabdomyosarcoma, and can be used as a novel molecular marker to be applied to detection of rhabdomyosarcoma. According to the method, by optimizing primers and an RT-PCR reaction system, the F-circP3F can be successfully amplified under the condition that the RNA content in the alveolar rhabdomyosarcoma cells and tissues is low, and the sensitivity and the specificity are high; and F-circP3F not only can be applied to diagnosis, classification, prognosis evaluation and the like of rhabdomyosarcoma, but also can provide a new way for epigenetic regulation and molecular diagnosis and treatment strategy research of rhabdomyosarcoma.
Owner:BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV

Methods and kits for diagnosing ulcerative colitis in a subject

InactiveUS20160230230A1Microbiological testing/measurementSLC19A3SLC22A3
The present invention relates to methods and kits for diagnosing ulcerative colitis in a subject. In particular, the present invention relates to a method for diagnosing ulcerative colitis in a subject comprising the steps consisting of determining in a sample obtained from the subject the expression level of at least one gene selected from the group consisting of ADH4, ADH6, ADHFE1, AKR1A1, AKR7A2, ALDH1A3, ALDH1L1, ALDH7A1, AOX1, BCHE, CBR3, CES1, CYP1B1, CYP2E1, CYP2W1, CYP4F11, CYP51A1, ESD, KCNAB2, COMT, GSTA4, GSTP1, INMT, MGST2, SULT2A1, TPMT, UGT1A4, UGT1A9, UGT2B7, ABCA1, ABCA2, ABCB1, ABCC1, ABCC10, ABCC5, ABCC6, ABCG2, ATP7A, SLC1A3, SLC7A5, SLC10A2, SLC15A1, SLC15A2, SLC19A2, SLC19A3, SLC22A3, SLC28A3, SLC29A2, SLC38A1, SLC38A5, SLC47A1, SLCO2B1, SLCO4C1, ARNT, FOXO1, HIF3A, NCOA2, NCOR2, NR1H3, NR3C1, PPARD, PPARGC1A, RARB, RXRB, and THRB.
Owner:INST NAT DE LA SANTE & DE LA RECHERCHE MEDICALE (INSERM) +3

Method for preparing of endothelial cells by transformation (transdifferentiation) of adult fibroblast, and use thereof

The present invention relates to a method of preparing vascular endothelial cells by transforming (transdifferentiating) adult fibroblasts and a composition, which includes vascular endothelial cells prepared according to the method, for preventing and treating ischemic diseases, the method including a step of transducing adult fibroblasts with a gene. In particular, the present invention confirms that five factors, Foxo1, Er71, Klf2, Tal1, and Lmo2, induce transdifferentiation of adult fibroblasts into induced vascular endothelial cells. Furthermore, the present invention confirms that three factors, Er71, Klf2, and Tal1 induce transdifferentiation of human adult fibroblasts into induced vascular endothelial cells. The resultant induced endothelial cells enable lower limb salvaging by angiogenesis in lower limb ischemic animal models, showing that the induced endothelial cells can be effectively used for prevention or treatment of ischemic diseases.
Owner:SEOUL NAT UNIV R&DB FOUND

Compositions comprising intermediate non-coding RNA regulators modulating the expression of etv6 or foxo1 and uses thereof

The present invention relates to: compositions for modulating the expression of FOXO1 and / or ETV6, the composition comprising one or more intermediate non-coding RNA regulators; attenuating splicing factor expression, the composition comprising an expression modulator of FOXO1 and / or ETV6; attenuating cell senescence and / or re-entry to cell cycle, the composition comprising an expression modulator of FOXO1 and / or ETV6 or their downstream targets related to splicing factor expression; or a composition capable of modulating splicing factor expression, the composition comprising one or more compounds able to bind to, or inhibit, FOXO1 and / or ETV6 genes or their downstream targets related to splicing factor expression. Such compositions have therapeutic benefits in the prevention, management, amelioration or treatment of an age-related disease or condition or cancer or also as a research tool / reagent.
Owner:SENISCA LTD

Grass carp FoxO1 polypeptide antigen and antibody and preparation method and application thereof

The invention relates to a grass carp FoxO1 polypeptide antigen and an antibody and a preparation method and application thereof, and belongs to the technical field of biochemistry and molecular immunology. According to the invention, the secondary structure, immunogenicity, hydrophilicity, hydrophobicity, surface probability and the like of a full-length functional protein amino acid sequence ofthe grass carp FoxO1 are analyzed, it is found that 15 amino acids of the grass carp FoxO1 polypeptide antigen, close to the N end, are strong in antigenicity, hydrophilicity and surface probability,therefore the 15 amino acids are used as the suitable grass carp FoxO1 polypeptide antigen. The polyclonal antibody for resisting the grass carp FoxO1 polypeptide antigen is prepared by immunizing NewZealand rabbits by taking the FoxO1 polypeptide antigen as an immunogen. The polyclonal antibody has the advantages of specifically recognizing the FoxO1 full-length functional protein in grass carptissues, filling in the blanks in the field of detection and research of the FoxO1 full-length functional protein of the grass carp, and laying a foundation for the research of regulating the fat metabolism function of an animal by the full-length functional protein of the grass carp FoxO1.
Owner:XINXIANG MEDICAL UNIV

Use of epigallocatechin gallate (EGCG) in preparation of food and drug for preventing and treating myocardial energy metabolism disorders

InactiveCN104042605APrevention and treatment of myocardial glucose metabolism disordersImprove myocardial functionOrganic active ingredientsMetabolism disorderAcute hyperglycaemiaDisease
The invention discloses a use of epigallocatechin gallate (EGCG) in preparation of a food and a drug for preventing and treating myocardial energy metabolism disorders. An EGCG action target is an intracellular FoxO1 signal channel. EGCG can inhibit myocardial energy metabolism disorders-caused myocardial cell autophagy and relieve myocardial mitochondria loss. EGCG has an effective concentration of 20 micromoles per liter to myocardial cells, or based on individual weight, an intake ratio of EGCG is 5-10mg / kg / day. EGCG can adjust a myocardial energy metabolism capacity, recover energy metabolism disorders-caused myocardial cell incompetence, inhibit myocardial cell H9c2 glycometabolism disorders-related autophagy increasing, inhibit myocardial cell H9c2 glycometabolism disorders-related oxidative stress, and reduce myocardial cell H9c2 glycometabolism disorders-related insulin resistance. EGCG as a food functional factor and a drug can be used for preventing and treating diseases such as myocardial damage caused by myocardial energy metabolism disorders, myocardial insulin resistance and hyperglycemia.
Owner:XI AN JIAOTONG UNIV

Method for directly transdifferentiating mesenchymal stem cells into sperms by using transcription factor FOXO1

InactiveCN111269940AUnderstanding the Regulatory MechanismFungiMicroorganism based processesMale infertilityINFERTILITY MALE
The invention provides a method for directly transdifferentiating mesenchymal stem cells into sperms by using a transcription factor FOXO1, comprising the following specific steps: introducing the transcription factor FOXO1 into the mesenchymal stem cells in a transfection mode, and carrying out induced culture on the mesenchymal stem cells to obtain the sperms. According to the method, the transcription factor FOXO1 is introduced into mesenchymal stem cells, and the mesenchymal stem cells are induced to develop towards sperms, so that not only can a regulatory mechanism of the stem cells on the vital movement of the organism be known, but also a signal path for maintaining the dynamic balance of the spermatogonial stem cells SSCs is discussed, and a new scheme for inheriting biological information of a male infertility patient, especially a male azoospermia patient, is provided.
Owner:SHENZHEN CHILDRENS HOSPITAL

Uses of glycerokinase as glucose metabolism disorder treatment target

The present invention relates to uses of glycerokinase as a glucose metabolism disorder treatment target. According to the present invention, the results show that glycerokinase can catalyze glycerol to generate 3-phosphoglycerol so as to participate in gluconeogenesis, can regulate the expressions of gluconeogenesis enzyme glucose-6-phosphatase (G6pase) and phosphoenolpyruvate carboxykinase (PEPCK) through an AKT-FOXO1 signaling pathway so as to regulate gluconeogenesis, and can provide important effects in the maintaining of the blood glucose homeostasis, such that the glycerokinase and the regulator thereof are the new target for blood glucose regulation and diabetes treatment.
Owner:SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI

Methods of diagnosing alzheimer's disease and risk of progression to alzheimer's disease

In one aspect, methods of diagnosing a subject as having Alzheimer's disease and prognosing a subject as being at risk of progressing to Alzheimer's disease are provided. In some embodiments, the method comprises determining one or more of the level of expression of rhotekin 2 (RTKN2), the level of expression of microtubule-associated Ser / Thr kinase 4 (MAST4), the level of binding of forkhead box O1 (FOXO1) to the RTKN2 promoter, and the level of binding of amyloid precursor protein (APP) to the MAST4 promoter in a sample from the subject.
Owner:LOMA LINDA UNIV HEALTH

Preparation method and application of cell, tissue or organ cold preservation liquid

ActiveCN112602703AImprove external cold storage effectImprove developmentDead animal preservationSomatic cellCoenocyte
The invention relates to the field of preservation of medical materials, in particular to a preparation method and application of a cell, tissue or organ cold preservation solution. According to a cold adaptation mechanism and a patent WO2019 / 040359 A1 discovered by early hibernation mode animal research of the inventor, extension is performed, and the in-vitro cold preservation effect of donor cells, tissues or organs is improved by regulating and controlling nucleus entry of FOXO1 through a medicine. Taking cornea cold preservation as an example, under the cold preservation strategy that C6 ceramide, Apigenin and Pi are added into the novel cornea preservation solution, corneal endothelial cells survive better, the death rate is only 2.5%, and the corneal permeability is improved. The quality of cornea in-vitro cold preservation is guaranteed, and more selectable cornea donors are provided for successfully carrying out a transplantation operation. The hibernation cold adaptation is based on effectiveness in different tissues and different species, and it is prompted that we can perform improved development of preserving fluid for different species and different organs.
Owner:THE THIRD AFFILIATED HOSPITAL OF SUN YAT SEN UNIV

Selective foxo inhibitors for treatment of diabetes and other disorders related to impaired pancreatic function

PendingUS20220185797A1Organic active ingredientsOrganic chemistryDiseasePANCREATIC FUNCTIONS
Various embodiments relate to a compound (represented by Formula I) or a pharmaceutically acceptable salt or tautomer thereof. The compound may selectively inhibit a Forkhead Box O1 (FOXO1) transcription factor. Various embodiments relate to methods comprising administering to a mammal having a disease or disorder associated with impaired pancreatic endocrine function, a therapeutically effective amount of the compound or a pharmaceutically acceptable salt or tautomer thereof. Various embodiments relate to methods for producing enteroendocrine cells that make and secrete insulin in a mammal, comprising administering to the mammal an effective amount of the compound or a pharmaceutically acceptable salt or tautomer thereof.
Owner:THE TRUSTEES OF COLUMBIA UNIV IN THE CITY OF NEW YORK +1

A drug for improving aortic endothelial cell function and its application

The disclosure relates to a composition for improving aortic endothelial cell function and use thereof. The composition includes acetic acid, lactic acid, polyoxyethylene castor oil, and disodium alkyl polyoxyethylene sulfosuccinate. The composition is capable of inhibiting inflammatory response of the human aortic endothelial cells caused by saturated fatty acids, increasing a mitochondrial respiration and metabolism of vascular endothelial cells, and preventing an occurrence and progression of atherosclerosis. The composition is capable of reducing human aortic endothelial inflammation caused by saturated fatty acids, for example, reducing the mRNA levels of interleukin-6 (IL-6) and matrix metalloproteinase-1 (MMP-1), and is capable of effectively protecting the function of mitochondria in human aortic endothelium from being damaged by saturated fatty acids, for example, increasing the expression of mitochondrial complexes I and III and mitochondrial metabolism-associated Foxo1.
Owner:XI AN JIAOTONG UNIV

Kit and method for detecting F-circP3F in rhabdomyosarcoma

The invention relates to the technical field of biomedical examination, in particular to a kit for detecting fusion gene related circular RNA-F-circP3F in paraffin-embedded tissue of rhabdomyosarcoma, which comprises a reagent for detecting a molecular marker F-circP3F of rhabdomyosarcoma and a fusion gene PAX3-FOXO1, a primer composition and a probe. The nucleotide sequence of the molecular marker F-circP3F is as shown in SEQ ID No. 1 (sequence identifier number 1). The invention provides the kit and the method for detecting F-circP3F in the paraffin-embedded tissue of rhabdomyosarcoma on the basis of one-step RT-PCR and qRT-PCR technologies, absolute quantitative detection of F-circP3F mRNA in the paraffin-embedded tissue can be realized, the operability and the repeatability are high, the kit and the method can be used for batch detection, the sensitivity and the specificity are relatively high, and the kit and the method can be used for detecting F-circP3F in the paraffin-embedded tissue of rhabdomyosarcoma. And diagnosis, typing and prognosis evaluation of rhabdomyosarcoma are facilitated.
Owner:BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV

Application of composition of demethylated magnesium cantharidate and sorafenib in preparation of anti-hepatoma drugs

ActiveCN113662939AImprove the effect of invasion and transferImprove the effect of inhibiting invasion and metastasisAntineoplastic agentsHeterocyclic compound active ingredientsOncologyMethyl palmoxirate
The invention discloses an application of a composition of demethylated magnesium cantharidate and sorafenib in preparation of anti-hepatoma drugs, the combined composition can effectively treat hepatoma, and the effect is obviously better than that of single drugs of demethylated magnesium cantharidate and sorafenib. The combination of the demethylated magnesium cantharidate and the sorafenib can significantly activate a transcription factor FOXO1 to inhibit invasion and metastasis of liver cancer cells, which indicates that the two have a synergistic effect, and the combined pharmaceutical composition provides a new therapeutic drug for clinical treatment of liver cancer, and has a good clinical application prospect.
Owner:ZUNYI MEDICAL UNIVERSITY

Composition for inhibiting calcification of endothelial progenitor cells and detection kit

The invention provides a composition for inhibiting calcification of endothelial progenitor cells and a detection kit. The composition for inhibiting the calcification of endothelial progenitor cellscomprises one of over-expression plasmids of Sox9, interference plasmids of ZBTB16, interference plasmids of FOXO1 and interference plasmids of Galectin-2 or a combination of at least two of the components above. The composition for inhibiting the calcification of endothelial progenitor cells can effectively reduce the formation of EPC mineralized nodules induced by an osteogenesis induction culture medium and simultaneously reduce the expression of a bone morphogenetic protein BMP-2. Furthermore, the provided detection kit can remind a user of the risk of vascular calcification, help to screen patients who need further detection, and improve the diagnosis and treatment efficiency.
Owner:XIN HUA HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE

Selective foxo inhibitors for treatment of diabetes and other disorders related to impaired pancreatic function

PendingCN113766915AOrganic active ingredientsOrganic chemistryDiseasePANCREATIC FUNCTIONS
Various embodiments relate to a compound (represented by Formula I) or a pharmaceutically acceptable salt or tautomer thereof. The compound may selectively inhibit a Forkhead Box O1 (FOXO1) transcription factor. Various embodiments relate to methods comprising administering to a mammal having a disease or disorder associated with impaired pancreatic endocrine function, a therapeutically effective amount of the compound or a pharmaceutically acceptable salt or tautomer thereof. Various embodiments relate to methods for producing enteroendocrine cells that make and secrete insulin in a mammal, comprising administering to the mammal an effective amount of the compound or a pharmaceutically acceptable salt or tautomer thereof.
Owner:THE TRUSTEES OF COLUMBIA UNIV IN THE CITY OF NEW YORK +1
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