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Molecular marker of rhabdomyosarcoma fusion gene related circular RNA and application of molecular marker

A technology of rhabdomyosarcoma and molecular markers, applied in the field of biomedical testing, can solve problems such as the specific mechanism to be elucidated, and achieve high detection rate and sensitivity

Active Publication Date: 2022-01-28
BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
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Problems solved by technology

The above studies have shown that F-circRNA produced by chromosomal translocation can lead to the development of tumors, but the specific mechanism remains to be elucidated
So far, there have been no reports of rhabdomyosarcoma-specific chromosomal translocations producing fusion gene-associated F-circRNAs

Method used

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  • Molecular marker of rhabdomyosarcoma fusion gene related circular RNA and application of molecular marker
  • Molecular marker of rhabdomyosarcoma fusion gene related circular RNA and application of molecular marker
  • Molecular marker of rhabdomyosarcoma fusion gene related circular RNA and application of molecular marker

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Embodiment 1

[0034] This embodiment provides a molecular marker of rhabdomyosarcoma fusion gene-related circular RNA, the marker is F-circP3F, and the nucleotide sequence of the marker is shown in SEQ ID No.1. The molecular markers can be used in the diagnosis of rhabdomyosarcoma products.

[0035]The design idea of ​​this application is: when the applicant uses RT-PCR technology to amplify the fusion gene PAX3-FOXO1 and its related circ RNA in the acinar RMS, it is speculated that PAX3-FOXO1 may be composed of intron 4 and intron 4 of PAX3 during the transcription process. Intron 2 of FOXO1 is complementary paired to form a circular lariat, and the intron is back-spliced ​​to form a circular RNA associated with the fusion gene. Then, by optimizing the primer design and linear RNA digestion, a positive target fragment was amplified in the acinar RMS cell line using divergent primers, and it was confirmed as a fusion by Sanger sequencing the PCR product and comparing it with the NCBI databa...

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Abstract

The invention provides a molecular marker of rhabdomyosarcoma fusion gene related circular RNA and application of the molecular marker. The molecular marker of the rhabdomyosarcoma PAX3-FOXO1 fusion gene related circular RNA is F-circP3F, and the nucleotide sequence of the molecular marker is as shown in SEQ ID No. 1. The F-circP3F provided by the invention has very high sensitivity and specificity to alveolar rhabdomyosarcoma, and can be used as a novel molecular marker to be applied to detection of rhabdomyosarcoma. According to the method, by optimizing primers and an RT-PCR reaction system, the F-circP3F can be successfully amplified under the condition that the RNA content in the alveolar rhabdomyosarcoma cells and tissues is low, and the sensitivity and the specificity are high; and F-circP3F not only can be applied to diagnosis, classification, prognosis evaluation and the like of rhabdomyosarcoma, but also can provide a new way for epigenetic regulation and molecular diagnosis and treatment strategy research of rhabdomyosarcoma.

Description

technical field [0001] The invention relates to the technical field of biomedical testing, in particular to a molecular marker of rhabdomyosarcoma fusion gene-related circular RNA and its application. Background technique [0002] Rhabdomyosarcoma (rhabdomyosarcoma, RMS) is the most common soft tissue malignancy in children and adolescents, accounting for about 50% of all soft tissue malignancies in children. According to clinical and histomorphological characteristics, it can be divided into four types: embryonal RMS (ERMS), alveolar RMS (ARMS), spindle cell RMS (SRMS) and pleomorphic RMS (PRMS). main types. They differ not only in age of onset, location, tissue morphology, etc., but also in treatment and prognosis. So far, studies have found that the occurrence and development of rhabdomyosarcoma (RMS) are closely related to molecular genetic changes such as the formation of chromosomal translocation fusion genes. The specific information on chromosomal translocations an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/113C12N15/11
CPCC12Q1/6886C12N15/113C12Q2600/158C12Q2600/178C12Q2600/112C12N2310/532
Inventor 李锋宋凌勰宋阳光贾兴元文豪张莹郭丹丹缪冉
Owner BEIJING CHAOYANG HOSPITAL CAPITAL MEDICAL UNIV
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