Grass carp FoxO1 polypeptide antigen and antibody and preparation method and application thereof

Abstract

The invention relates to a grass carp FoxO1 polypeptide antigen and an antibody and a preparation method and application thereof, and belongs to the technical field of biochemistry and molecular immunology. According to the invention, the secondary structure, immunogenicity, hydrophilicity, hydrophobicity, surface probability and the like of a full-length functional protein amino acid sequence ofthe grass carp FoxO1 are analyzed, it is found that 15 amino acids of the grass carp FoxO1 polypeptide antigen, close to the N end, are strong in antigenicity, hydrophilicity and surface probability,therefore the 15 amino acids are used as the suitable grass carp FoxO1 polypeptide antigen. The polyclonal antibody for resisting the grass carp FoxO1 polypeptide antigen is prepared by immunizing NewZealand rabbits by taking the FoxO1 polypeptide antigen as an immunogen. The polyclonal antibody has the advantages of specifically recognizing the FoxO1 full-length functional protein in grass carptissues, filling in the blanks in the field of detection and research of the FoxO1 full-length functional protein of the grass carp, and laying a foundation for the research of regulating the fat metabolism function of an animal by the full-length functional protein of the grass carp FoxO1.

Description

technical field [0001] The invention relates to a grass carp FoxO1 polypeptide antigen, an antibody and a preparation method and application thereof, belonging to the technical fields of biochemistry and molecular immunology. Background technique [0002] Adipose tissue is the largest energy storage place in animals and plays a key role in energy balance in the body. Unbalanced development of adipose tissue often leads to many health problems. The location of fat deposition may be determined by tissue-specific lipid metabolism pathways, which are regulated by key enzymes and genes at the molecular level. Therefore, it is imperative to use molecular biology techniques to study the molecular mechanism of functional genes regulating fat metabolism. [0003] Grass carp is a unique freshwater fish in my country. It belongs to one of the "four major fishes" and has high economic value. Most farmed fish (especially grass carp) will experience problems such as fat accumulation, li...

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Problems solved by technology

[0007] The present invention also provides an antibody against grass carp FoxO1 polypeptide antigen, which can specifically recognize FoxO1 full-length functional protein in grass carp tissue, and can be used to detect the expression level of FoxO1 full-length functional protein in grass carp fat, liver and muscle tissue , solve the problem of lack of commercial grass carp FoxO1 antibody in the prior art, and fill the gap in the field of grass carp FoxO1 full-length functional protein detection

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