358 results about "INFERTILITY MALE" patented technology

The present invention discloses methods for isolating, proliferating and differentiating germ-line stem cell in vitro. More specifically, the present invention discloses a method for the in vitro isolation and proliferation of mammalian germ-line stem cells, characterized in culturing cells isolated from mammalian testis in an embryonic stem cell culture medium, and for the in vitro differentiation, characterized in encapsulating mammalian germ-line stem cells and Sertoli cells with calcium alginate and co-culturing with peritubular cells. Also, the present invention discloses germ-line stem cells obtained by above methods, a composition for the treatment of male infertility, comprising the germ-line stem cells, and a method for the treatment of male infertility by the use of the germ-line stem cells.

The invention discloses a method for diagnosing male infertility, and discloses a biological marker indicating male infertility, a method for indentifying and detecting the biological marker indicating male infertility, and a kit for diagnosing or detecting male infertility. The method combined with seminal fluid routine inspection helps to realize the accurate diagnosis on male infertility.

The present invention relates to a novel method of treating male infertility such as, for example, idiopathic asthenospermia. In one aspect the present invention relates to the treatment or prevention of male infertility through the administration of a composition containing ubiquinone, ubiquinol or both, and optionally, a member chosen from the group consisting of a reducing agent, a solubilizer, an emulsifier, a surfactant, a triglyceride, an oil, a fat, a lipid, a phospholipid, a carbohydrate, an antioxidant, a carnitine, creatine, an herbal extract, a vitamin, a coenzyme, a mineral, an electrolyte, a pharmaceutical, a water, a carrier, an excipient, and any combination thereof.

A sperm-producing decoction is disclosed and warms and invigorates kidney yang. The decoction is used for patients with male infertility caused by insufficiency of the kidney yang, low sperm count, azoospermia, or a sperm amount lower than a normal value, and low sperm motility. A traditional Chinese medicine composition is prepared from following raw material medicines by weight: prepared rehmannia root, Chinese wolfberry, raspberry, common dodder, pulp of dogwood fruit, fruit of magnoliavine, semen plantaginis, epimeddium and morinda root. The sperm-producing decoction has good effects for azoospermatism, oligospermia, and normal sperm count type infertility, and is good in effects and quick-acting in curative effects for male infertility. Natural traditional Chinese components are free of side effects for human bodies. The sperm-producing decoction has functions of strengthening sperms and effectively increasing the amount of sperms.

The invention discloses a medicament for treating male infertility, which is prepared from astragalus root, Siberian solomonseal rhizome, epimeddium, cnidium fruit, plantain seeds, ginseng, raspberry, deer horn gum, asparagus root, poria cocos, leek seeds, broomrape, anemarrhena rhizome and eucommia bark through steps of disintegrating, grilling, extracting concrete, and the medicament can be prepared into powders, granules, tablets or capsules.

The invention relates to a corning pill which comprises plantain seed, fringed pink, polygonum aviculare, soapstone, earthworm, seaweed, laminaria, pangolin, red flower, peach kernel, honeysuckle, cordate houttuynia, gardenia, scutellaria baicalensis, agilawood, fructus amomi, cowherb seed, elecampane, mangnolia officinalis and fructus aurantii. The corning pill has the major functions of treatingmale genitourinary system diseases including male infertility, prostatitis, orchitis, seminal vesiculitis, male and female urethritis, cystitis and the like.

The invention provides a kit for male azoospermia diagnosis and a use method. The kit contains biological marker detection reagents for measuring different expression levels of plasma miR-15b, and azoospermia is prompted by excessive expression of the miR-15b. Tiny RNA (ribonucleic acid) genes miR-15b playing an important role in the sperm generation process are discovered, the miR-15b is differently expressed in male infertility patients, and significant expression change of the miR-15b can be detected in the plasma. Based on the principle of quantitative PCR (polymerase chain reaction), and the kit is fine in result repeatability, objective, accurate and easily popularized in different detection mechanisms, hospital laboratories, male laboratories and the like.

The invention provides a method for detecting neuraminidase influencing sperm functions. The method is a novel laboratory detecting method for assessing quality and functions of a sperm and can provide detection and clinical consultations for male infertility patients without clear clinical reasons for infertility. The method is characterized in that expressions of neuraminidase in the sperm are visually quantized through single sperm fluorescence strength analysis or are visually observed by means of fluorescence imaging under a microscope, and if the neuraminidase is highly expressed in the sperm, capacitation of the sperm is achieved through external capacitation liquid, and neuraminidase activity is detected in a fluorescence method.

The invention relates to a traditional Chinese medicine composition used for treating male sterility, a preparation method and a quality control method thereof. The traditional medicine composition contains crude drugs such as fruits of Chinese wolfberry, dodder (fried), raspberry, fructus schizandrae (steamed), plantago seeds (fried with salt) and the like. The preparation method comprises an ultrafine grinding technology and the like. The quality control method of the medicine composition comprises a thin-layer identification method for fruits of Chinese wolfberry, dodder, raspberry, fructus schizandrae and plantago seeds as well as a method for measuring the content of schizandrin B in the plantago seeds. The medicine composition has obvious therapeutic effect in treating renal deficiency and lumbago, post-micturition dribble, spermatorrhea, premature ejaculation, impotence and sterility.

The invention provides a method for diagnosing male infertility in unknown aetiology and relates to an analytical test of a body fluid sample. In certain aspects, a biomarker for indicating male infertility in unknown aetiology is included; a method for identifying and detecting the biomarker is included; a kit for diagnosing or detecting male infertility in unknown aetiology is also included. According to the diagnosing method, the simple, accurate and non-invasive diagnosis of male infertility in unknown aetiology can be realized.

The present invention relates to a cistanche health-care tea and its processing method. Its raw material composition includes (by wt%) 30-36% of cistanche stem, 8-10% of curculigo root, 10-15% of polygonatum root, 8-10% of epimedium, 8-10% of lyceum berry and 30-36% of tea. Its processing method includes the following steps: selecting raw materials, cleaning, pulverizing, stirring, granulating and drying. It can be extensively used for curing the diseases of impotence due to insufficiency of the kidney-yang, female sterility, intolerance of cold, lumbago, male infertility due to insufficiency of essence and blood and constipation, etc.

The present invention related to a functional briefs for men is characterized in natural separation and storage of penis and scrotum without any measures or restrictions when wearing the functional briefs for men, and no escape of penis and scrotum from the containing pocket without regard to the shrinkage or the expansion of the penis and the scrotum, or exercise or daily activities. The characteristics explained hereinbefore are advantageous in having a fresh sense of touch in the briefs and in preventing the uncomfortable feelings of the scrotum and male infertility due to sweat and increasing temperature.

The invention discloses a receipt of gypenoside lipidosome consisting of sterol. The receipt comprises gypenoside, a phosphatide material and the sterol. The invention further discloses a preparation method of the gypenoside lipidosome consisting of the sterol. A preparation form of the lipidosome can be a freeze-drying injection, an injection, a transdermal preparation, aerosol, oral liquid, a solution, an eye-drops preparation, a tablet or a capsule. The gypenoside lipidosome has important treatment effects for hyperlipidemia and cardiovascular and cerebrovascular diseases, and furthermore, has positive treatment and prevention effects for tumor, hepatitis, gastritis, gastric and duodenal ulcer, gastroptosis, stomatitis, diabetes mellitus, astriction, haemorrhoids, asthma, cephalagra, acne, stain, gall-stone, numbness of limbs, pachulosis, male infertility, recession of sexual functions, fatness, white hair and baldness. Furthermore, the physical strength of a human body can be enhanced, the immunity of the organism is improved, the state of fatigue can be relieved, the sleep quality can be obviously improved, the appetite can be whetted, and the senescence can be delayed.

The invention relates to a composite traditional Chinese preparation medicine for treating male infertility, impotence and premature ejaculation and a preparation method thereof, and in particular relates to a yang-tonifying traditional Chinese medicine for generating and strengthening sperms, which is prepared by a plurality of plant Chinese medicines and less animal traditional Chinese medicines as materials for treating the diseases such as aspermia, oligospermia, weak sperm, teratospermia, non-liquefied semen, impotence, premature ejaculation and sexual dysfunction in male infertility, belonging to the technical field of traditional Chinese medicine production. The yang-tonifying traditional Chinese medicine is prepared by the following materials in parts by weight: 0.5-1.5 parts of bloody velvet deer antler, 5-10 parts of morinda officinalis, 6-12 parts of Chinese yam, 5-10 parts of fruit of Chinese wolfberry, 5-10 parts of cortex moutan, 5-15 parts of radix rehmanniae preparata, 5-10 parts of pulp of dogwood fruit, 5-15 parts of poria, 5-10 parts of rhizoma alismatis, 5-15 parts of cuscutaceae, 5-10 parts of raspberry, 2-6 parts of rhizoma curculiginis, 5-10 parts of herba epimedii and 2-10 parts of schisandra chinensis. Water is directly added to the components for boiling the mixture to be decoction, or crushing and mixing the components into powder, capsules or pills. The yang-tonifying traditional Chinese medicine provided by the invention has a simple preparation process, a remarkable effect, no toxic or side effect, an effective rate of 100%, a cure rate of 85% of after being taken for one month, and a curate rate of 91% after being taken for two months.

The invention discloses a method for breeding Medicago sativa L. male sterile line. The method is characterized by comprising the following steps of: performing interspecific distant hybridization on wild Medicago falcata L. from Xilin Gol grassland of Inner Mongolia which is used as female parents purple alfalfa from Jungar grassland of Inner Mongolia which is used as male parents; obtaining male sterile line Ms-4 from the offsprings; back crossing four male parents with the male sterile line Ms-4; performing cuttage propagation on the male sterile plants in each back cross offspring, and testing the combining ability of the agronomic traits for further selecting two excellent male sterile lines of the Medicago sativa L. with high combining ability and high volume of production from the male sterile plants, wherein the two excellent male sterile lines are named Ms-4-1 and Ms-4-2. The sterile lines obtained by the method have high combining ability, excellent fertility, high male infertility rate up to 100% and stable heredity.

The invention discloses an epididymal protease inhibitor protein monoclonal antibody and application thereof, which belong to the field of biological engineering. The monoclonal antibody is secreted by hybridoma preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of C200849. The monoclonal antibody can be combined to the epididymal protease inhibitor protein Eppin protein of specific expression in a testis and epididymis tissue by specificity. The monoclonal antibody can be used for preparing male infertility diagnostic reagents, male contraceptives and anti-fertility study biological products.

The invention belongs to the fields of analytical chemistry and clinical medicine, and discloses a urine fatty acid metabolite marker related to idiopathic male infertility, as well as a detection method and application thereof. The marker is urine fatty acid metabolites, namely, tetradecanedioic acid and / or hexadecanedioic acid, and is detected by adopting a UPLC-Q exactive MS method; the marker can be used for auxiliary diagnosis and monitoring of the idiopathic male infertility, has high sensitivity and specificity, and has a clinical popularization value.

The invention belongs to the technical field of vegetable molecular breeding, and particularly relates to an SNP molecular marker related to infertility of capsicum and application thereof. The SNP molecular marker related to the infertility of the capsicum is SNP2, SNP3, SNP4, SNP7 or SNP8, the nucleotide sequence of an SNP site is shown in SEQ ID NO.1-5, and the molecular marker is co-separatedor closely linked to pepper fertility phenotype. The invention also provides a KASP primer for identifying the above SNP molecular marker; the primer can be used for rapidly identifying the pepper fertility phenotype, and can greatly accelerate the transfer process of a novel capsicum male infertility line. The SNP molecular marker related to the infertility of the capsicum developed belongs to acosegregation SNP marker of a nuclear infertility gene, and the plant fertility can be determined in the seedling stage of a pepper, thereby reducing the seed production cost. The marker can also accelerate the transfer process of the novel capsicum male infertility line.

The invention discloses an application of a traditional Chinese medicine composition in preparing a medicament for treating male infertility. The traditional Chinese medicine composition improves a microenvironment for generating sperms by promoting the blood circulation and removing meridian obstructions so as to achieve the purpose of effectively treating the male infertility.

The invention discloses a method for efficiently separating mouse spermatogonial stem cells. The method includes the following steps that firstly, mouse testis are collected, and a testicular cell suspension is prepared through a two-step enzyme digestion method; secondly, the testicular cell suspension is inoculated in a porous plate according to a certain density, and a spermatogonial stem cell culture solution is added for in-vitro culture; thirdly, the culture solution is removed, testis cells of different times are cultured through tryptic digestion, and spermatogonial stem cells are sorted from the testis cells through the magnetic activated cell sorting; fourthly, the sorted spermatogonial stem cells are inoculated to an STO trophoblast, and a spermatogonial stem cell culture solution is added for primary culture and subculture of the spermatogonial stem cells. A mouse spermatogonial stem cell system is built through the method including the steps of separation, culture, re-separation and re-culture. The method can achieve the aim of efficiently enriching the spermatogonial stem cells in vitro, and is economical and easy to implement, so that a direction is provided for protection of rare animal varieties and treatment of male infertility.

The invention discloses a gene detection method of a sperm cell apoptosis related gene. The gene detection method is characterized in that detection of the sperm cell apoptosis related gene is provided for all subjects by simultaneously detecting and analyzing SNPs site gene portability of individual NOS3 gene and FASLG gene, and forecast evaluation on a male infertility risk is carried out by combining with unique environmental exposure factors and lifestyle factors related to azoospermia and oligospermia in Chinese population.

The invention discloses a kit and a PCR amplification method for detection of one pathogenic mutation site of a male infertility Dmc1 gene. The method is characterized in that a modified mutation-detection primer resistant to 3'-5' exonuclease enzyme digestion is used in a PCR reaction in which a high-fidelity DNA polymerase participates, so that the specificity of PCR is improved; and the existing condition of a gel electrophoresis product band can be directly observed to judge the genotype of the Dmc1 gene corresponding mutation site. The detection kit has low requirements on instruments and equipment, is simple in operation, is economic, has no need of sequencing, and can be operated in common molecular biology laboratories.

The invention belongs to the field of analytical chemistry and clinical medicine and discloses an idiopathic male infertility-related medium-chain fatty acid marker in serum and a detection method and application thereof. The marker is capric acid and / or lauric acid, and an UPLC-Q exactive MS method is adopted for detection. The marker can be used for auxiliary diagnosis and monitoring of idiopathic male infertility, has high sensitivity and specificity, and has clinic promotion value.

This invention pertains to the discovery that stem cells (e.g., bone marrow stem cells) transplanted directly into a testicular environment are transdifferentiated into bona fide Sertoli cells, and / or Leydig cells, and / or and germ cells. This provides a mechanism for the treatment of male infertility and / or testosterone deficiency. Thus, in one embodiment, this invention provides a method of treating infertility or testosterone deficiency in a male mammal. The method typically involves implanting stem cells into the testes of the mammal whereby the stem cells differentiate into germ cells and / or Sertoli cells and / or Leydig cells thereby reducing infertility and / or testosterone deficiency.

The invention provides herba epimedii soup. The soup has the functions of warming the kidney, replenishing essence, and invigorating qi and blood. According to the main function, the soup is rapid in curative effect and excellent in effect on male infertility caused by too many dead sperms. A traditional Chinese medicine composition is formed by compatibility of a dozen of medicinal materials including, by weight, herba epimedii, cistanche, Semen Cuscutae, glossy privet fruits, the fruits of Chinese wolfberry, prepared fleece flower roots, radix paeoniae alba, Angelica sinensis, pulp of dogwood fruits, dipsacus roots, Eclipta alba, Astragalus membranaceus, semen plantaginis and liquorice. The soup aims at the male function, has the functions of warming the kidney, replenishing essence, and invigorating qi and blood, and is rapid in curative effect on the male infertility caused by the too many dead sperms, natural Chinese herbal medicinal ingredients have no side effect on the human body, and the formula can enhance sperm motility and can effectively increase the number of sperms.

The invention belongs to the fields of analytical chemistry and clinical medicine, and discloses an idiopathic male infertility-related seminal plasma metabolic small molecular marker as well as a detection method and application thereof. The marker is prepared from seminal plasma metabolic small molecular acetylcarnitine and / or tryptophan, and is detected by adopting a UPLC-Q exactive MS method; the marker can be used for auxiliary diagnosis and monitoring of the idiopathic male infertility, and has higher sensitivity and specificity, thus having a clinical popularization value.

Disclosed is a medicine for treating male infertility, which mainly comprises curculigo rhizome, epimeddium, raspberry, leek seeds, dodder seed, wolferry fruit, plantain seeds, schisandra fruit, mulberry seeds, pilose asiabell root, and licorice root. The medicine is made into the dosage form of oral liquid.

The invention provides a health care belt which comprises a belt body, tourmaline ceramic powder, magnetic iron oxide and a medicine bag, wherein the tourmaline ceramic powder, the magnetic iron oxide and the medicine bag are attached to the belt, and medicine in the medicine bag is composed of a mixture of the original medicine materials and extractives of teasel root, Asclepiadaceae, achyranthes bidentata, fructus evodiae, poria cocos, star-flower lysimachia, semen cassiae, gynostemma pentaphylla, edgeworthia chrysantha lindl root, epimedium and Rhizoma Drynariae. The health care belt fully utilizes thermal therapy effect of infrared heat dissipation of human body, and fully plays medicine activity of the medicine bag. The medicine bag in the health care belt also has good activity in male infertility treatment.

The diagnosis of male infertility is based predominantly on the results of standard semen analysis for concentration, total motility, progressive motility, volume, pH, viscosity and / or morphology. When sperm enter the female reproductive tract, they must undergo a series of physiological changes, known as capacitation, in order to fertilize an egg. This process involves plasma membrane changes that occur in response to stimuli within the female tract. These changes include removal of sterols and redistribution of the ganglioside GM1. Semen analysis identifies only half the cases of male infertility due to standard semen analysis providing little information on sperm functional competence. Previous data demonstrated that localization of the ganglioside, GM1, identifies sub-populations of sperm capable of undergoing the functional maturation process known as capacitation and tracks strongly with fertility.

The invention discloses Chinese medicinal powder for treating male infertility with kidney essence deficiency, which is characterized in that: the Chinese medicinal powder is prepared from the following raw materials by weight: 10 to 14 grams of hairy antler, 10 to 14 grams of medlar, 8 to 12 grams of panax ginseng, 8 to 12 grams of Chinese caterpillar fungus, 13 to 17 grams of south dodder seed, 13 to 17 grams of epimedium brevicornum, 11 to 15 grams of siberian solomonseal rhizome, 16 to 20 grams of tuber fleeceflower root, 18 to 22 grams of mulberry, 10 to 14 grams of common curculigo rhizome, 8 to 12 grams of eucommia bark, 18 to 22 grams of european verbena and 13 to 17 grams of ginkgo leaf. The Chinese medicinal herb of the invention has the effect of tonifying kidney and yang and increasing essences and promoting fertility through organic combination, syndrome differentiation and treatment and mutual supplement and complement to fulfill the aim of treating the male infertility with kidney essence deficiency. 978 men who suffer from the male infertility with kidney essence deficiency are treated clinically, and the total effective rate reaches 92.2 percent. The Chinese medicinal powder has the advantages of low cost, high cure rate, no side effect and convenient administration.