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Method for breeding Medicago sativa L. male sterile line

A male-sterile line and male-sterile technology, which is applied in the field of breeding of alfalfa male-sterile lines, can solve problems such as rareness, and achieve the effects of improving the fertility of pistils, reducing the probability and increasing the seed setting rate.

Inactive Publication Date: 2011-08-10
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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However, reports on related breeding methods and patent releases are rare

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  • Method for breeding Medicago sativa L. male sterile line

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Embodiment Construction

[0018] Enumerate the embodiment of a selection method below in conjunction with accompanying drawing: 1, in grassland No. 1 miscellaneous alfalfa (with the wild yellow alfalfa of Inner Mongolia Xilin Gol grassland as female parent, Zhungeer alfalfa is male parent hybridization) flowering stage, field Check the anthers of each plant. If the anthers are found to be shriveled, there is no pollen or the plants with very little pollen are tagged, and the codes are marked in sequence, and 6 male sterile plants are selected from an average of 70,000 to 100,000 plants. One male sterile plant was produced.

[0019] 2. The pollen fertility of 6 sterile plants was repeatedly identified, and 3-4 inflorescences were cut from each plant tagged in the field, brought back to the room, observed under a microscope and tested for pollen fertility. Among them, the volume of fertile pollen grains is larger, 3-4 times that of sterile pollen grains, and the pollen grains are filled with nuclei and t...

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Abstract

The invention discloses a method for breeding Medicago sativa L. male sterile line. The method is characterized by comprising the following steps of: performing interspecific distant hybridization on wild Medicago falcata L. from Xilin Gol grassland of Inner Mongolia which is used as female parents purple alfalfa from Jungar grassland of Inner Mongolia which is used as male parents; obtaining male sterile line Ms-4 from the offsprings; back crossing four male parents with the male sterile line Ms-4; performing cuttage propagation on the male sterile plants in each back cross offspring, and testing the combining ability of the agronomic traits for further selecting two excellent male sterile lines of the Medicago sativa L. with high combining ability and high volume of production from the male sterile plants, wherein the two excellent male sterile lines are named Ms-4-1 and Ms-4-2. The sterile lines obtained by the method have high combining ability, excellent fertility, high male infertility rate up to 100% and stable heredity.

Description

technical field [0001] The invention relates to a forage breeding method, in particular to a method for selecting male sterile lines of alfalfa. Background technique [0002] Alfalfa (Medicago sativa L.) is one of the most widely planted leguminous forages in the world. There are more than 60 species in the world, and there are 16 species in my country. Because its nutritional value and forage yield are obviously superior to other grasses, it is called the "king of forage grass". In addition, alfalfa has a well-developed root system, root nodules fix nitrogen, improve soil and fertilize fields, and has excellent ecological functions. With the development of animal husbandry, the construction of the ecological environment and the promotion of the commodity economy, the alfalfa planting area has been expanding year by year in recent years, and its industrialization scale has developed rapidly. By 2008, the national alfalfa planting area reached 4.13 million ha 2 (Lu Xinshi 2...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 石凤翎高翠萍蔡丽艳高霞吴永敷云锦凤米福贵李志勇
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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