56 results about "Pistia" patented technology

The invention provides a method with effects of saving cost and improving efficiency for liquid pollination of pear tree, which belongs to the fruit trees production technical field. The method comprises the following steps: preparing a nutrient solution in full-bloom stage of pear flowers, adding 0.8g pure pollen in every liter of nutrient solution, spraying and pollinating by an electrostatic sprayer to achieve the purpose of artificial pollination. The pollination method has the advantages of simple operation, fast pollination speed, uniform powder injection, concentration, accuracy, pollen and manual work saving, low cost and the like, and solves the practical problems that pollen can not be uniformly dissolved in water in liquid pollination of fruit trees, pollen is easy to adhere with the wall of a container, pollen is obstructed a nozzle, uneven powder injection causes poor pollination effect and the real application of liquid pollination can not be realized for a long time. According to the invention, the new collected pure pollen (germination rate is 60%-80%) in current year required by per mu is 8-11g. In addition, the method of the invention is capable of increasing the moisture and nutrient of pistil stigma and prolonging the fecundation time of stigma pollination, and the fruit quality can not be affected by increasing the fruit setting rate.

The invention discloses Taiwania flousiana Gaussen with herbicidal activity and an application of Taiwania flousiana Gaussen and an application of a Taiwania flousiana Gaussen extract as a herbicide. The research finds that the Taiwania flousiana Gaussen and the Taiwania flousiana Gaussen extract have the efficacies of remarkably inhibiting germination, synthesis of chlorophyll a and growth of multiple classical farmland weeds, invasive weeds and aquatic weeds such as crab grass, amaranthus retroflexus, tropic ageratum herb, feather cockscomb, Bidens pilosa, mikania micrantha, Pistia stratiotes and spirogyra, have remarkable inhibition effects, can be applied to the prevention and kill-off of the farmland weeds and can be developed into efficient and pollution-free environment-friendly herbicides, or a lead compound is provided for the creation of novel herbicides; and the Taiwania flousiana Gaussen and the Taiwania flousiana Gaussen extract have remarkable social and economic benefits and good application prospects.

The invention provides a plant restoration method for uranium polluted water, and the method is characterized in that a floating aquatic plant Pistia stratiotes L is stocked in uranium polluted water, the plant root can quickly absorb and concentrate uranium in large amounts, simultaneously some uranium is transferred upward to leaves above water, through timing salvage, collection, and concentrated treatment, large amounts of uranium is removed from water, and after a certain restoration period, the uranium content in water can decline to the scope which is allowed in drinking water standard. The initial concentration of uranium is 1500 micrograms per liter; the proportion of added Pistia stratiotes L is about 1/5 of the water weight; after one day, the removing rate exceeds 98%, and the uranium concentration effectively declines to be lower than the drinking water standard (30 micrograms per liter). The method restores uranium polluted water quickly, purifies water thoroughly, and is suitable for various uranium polluted water. The method has no special requirement for equipment and a place to be restored, and is simple and easy for use.

The invention discloses a method for breeding Medicago sativa L. male sterile line. The method is characterized by comprising the following steps of: performing interspecific distant hybridization on wild Medicago falcata L. from Xilin Gol grassland of Inner Mongolia which is used as female parents purple alfalfa from Jungar grassland of Inner Mongolia which is used as male parents; obtaining male sterile line Ms-4 from the offsprings; back crossing four male parents with the male sterile line Ms-4; performing cuttage propagation on the male sterile plants in each back cross offspring, and testing the combining ability of the agronomic traits for further selecting two excellent male sterile lines of the Medicago sativa L. with high combining ability and high volume of production from the male sterile plants, wherein the two excellent male sterile lines are named Ms-4-1 and Ms-4-2. The sterile lines obtained by the method have high combining ability, excellent fertility, high male infertility rate up to 100% and stable heredity.

The invention provides a method for performing artificial crossbreeding without removing stamen from catalpa bungei. The method particularly comprises the following steps: (1) collecting pollen: collecting anther before the anther of the catalpa bungei is mature and does not crack, drying the anther indoors at the room temperature of 20 to 22 DEG C and collecting pollen after the anther cracks; and (2) performing artificial pollination: when the stamen of each flower of catalpa bungei is mature, the stigma is opened in a reverse V shape and the surface has milk white luster, lightly touching with hands or shaking catkin and quickly closing to perform artificial pollination. By the method, the artificial pollination is properly performed by selecting the optimum pollination time at the appropriate air temperature and under the clear, windless or breeze condition, so that a seed tree can be fertilized successfully and fructify a large number for fruits, and the requirements of scientific research and production can be met.

The invention discloses a soybean MADS-box gene and applications thereof in floral organ modification, belonging to the field of biotechnology. GmMADS1, transcription factor in nucleus, is expressed in all the floral organs of four circles and, however, expressed in large quantity in petals and stamens, tobacco expressing excessive GmMADS1 increases the number of sepals, stamens and petals, a transformation from the sepals and the stamens to the petals is formed, as a result, the occurrence of cracking petals and bending stamens etc. causes the stamens to fail to come into contact with pistils, thus normal pollination and normal anther dehiscence cannot be realized and pollen activity is lowered. The above results represent that GmMADS1 plays an important regulation and control role in determining the position and the number of the petals and the development of male organs. New floral organ-variant material, resulted from the GmMADS1 gene, can be used for the breeding of crops and ornamental horticulture plants.

The present invention relates to a method for external regeneration and propagation method of pistia stratiotes. Said method includes the following steps: selecting pistia stratiotes stem, cleaning and disinfecting it to obtain sterile explant; cutting its stem into slices, then placing the slice on the MS culture medium containing plant hormone 2,4-D and BA, inducing to form callus; then transferring said callus onto the MS culture medium containing plant hormone BA to make regeneration and obtain seedling, further rooting so as to obtain whole regenerated plant, then transferring the regenerated plant onto SH liquid culture medium to make extensive propagation.

The present invention provides a Chinese cabbage pistil development related gene BrCRF11a and an application thereof, and belongs to the technical field of plant genetic engineering. A DNA sequence ofthe Chinese cabbage BrCRF11a is shown as SEQ ID No. 1 and a gene artificial miRNA sequence is shown as SEQ ID No. 2. The gene and the artificial miRNA designed for the gene are respectively transferred into brassicae parachinensis bailey, var. Youqing-Siqiu by an agrobacterium-mediated method to obtain transgenic brassicae parachinensis strains with overexpression and inhibition expression of BrCRF11a, expression changes of Chinese cabbage BrCRF11a enable the brassicae parachinensis to show a phenotype of nested flowers and the phenotype is caused by continuous maintenance of stem cell activity by floral meristem. The Chinese cabbage BrCRF11a is closely related with pistil development. The gene is applied in Chinese cabbages and other horticultural plant breeding and has good applicationprospects.

A process and composition for thinning fruit blossoms with a calcium chloride solution is provided. The calcium chloride is a common salt, often referred to as CaCl, but having the chemical formula CaCl2 and is preferably applied to the blooms in a solution or brine. The calcium chloride brine is a natural material that is classified as a "generally regarded as safe" (GRAS) compound. The calcium chloride solution in water apparently interrupts the pollination of blooms through the dessication of the pistils, when it is applied during the blooming period. The CaCl2 blossom or bloom thinning agent solution is effective on many varieties of fruit trees, especially apples. The calcium chloride is preferably applied in a substantially aqueous solution at a rate of between 5 and 40 pounds of calcium chloride per acre. The CaCl2 solution is not an artificial or derived substance and carries none of the environmental and health concerns raised by other hormonal or caustic chemical thinning agents.

The invention discloses C-glycosyl transferase and application thereof in synthesis of schaftoside and isoschaftoside. 2-hydroxyflavanone C-glycosyl transferase genes (SbCGTa, GuCGTa, PsCGTa, LpCGTa and AeCGTa) and C-glucose-2-hydroxyflavanone C-glycosyl transferase genes (SbCGTb, ZmCGTb, OsCGTb1, OsCGTb2, GuCGTb, PsCGTb, LpCGTb and AeCGTb) are separated from scutellaria baicalensis, corn, rice, licorice, pistia stratiotes, spirodela oligorrhiza and arisaema erubescens. The invention finds for the first time that proteins coded by the genes participate in synthesis of phytoalexin schaftoside and isoschaftoside in plants, so that a feasible method is provided for synthesizing the schaftoside, the isoschaftoside and analogues thereof by utilizing enzyme catalysis in vitro.

The invention relates to an aristolochia debilis artificial pollination method which is characterized by comprising the following steps of: when the aristolochia debilis blooms, irradiating the stamen by a filament lamp so that the anther cracks and the stamen pollen spills out; collecting the pollen and drying in the air, and keeping in a dark place for later use; pollinating when the pistil matures and the pedal becomes dark red and the flower emits the smell similar to slough; adding 50% of starch into the collected pollen, and lightly shaking and uniformly stirring; feeding the mixture into a pollinator; and holding the aristolochia debilis flower diameter with a hand, aligning the aristolochia debilis flower diameter with the horn-shaped perianth cylinder, and spraying the pollen into the perianth cylinder, wherein when the fur in the perianth cylinder becomes soft and withers, the pollination is finished. The method provided by the invention is simple to operate, and effectively solves the problems of difficult aristolochia debilis pollination and low fruit setting rate, so that the artificial planting output and economic benefits of the aristolochia debilis are remarkably increased.

The invention relates to the field of molecular biology, and in particular to catalpa bungei CabuAP3 protein as well as an encoding gene and application thereof. The amino acid sequence of the catalpa bungei CabuAP3 protein is as shown in SEQ ID No.1; the sequence (CDS) of the encoding gene is as shown in SEQ ID No.2; the whole length of the cDNA sequence of the catalpa bungei CabuAP3 gene is as shown in SEQ ID No.3. The catalpa bungei CabuAP3 gene provided by the invention is only expressed in petals and stamens of catalpa bungei, and is not expressed in leaves, sepal or pistil. A CabuAP3 gene expression vector is transferred into mutants of wild arabidopsis and arabidopsis ap3-3, the result shows that the transgenic wild arabidopsis has one extra petal, which indicates that the CabuAP3 gene has the function of regulating and controlling increase of the number of petals of the transgenic wild arabidopsis, and the transgenic wild arabidopsis ap3-3 mutant has a filament structure. The plant variation material obtained from the catalpa bungei CabuAP3 gene expression vector can be applied to plant breeding and view admire, and has very good application prospect.

The invention belongs to the technical field of environmental remediation, and particularly relates to an adsorbent and a preparation method and application thereof. The preparation method of the adsorbent comprises the following steps of: step 1, mixing pistia stratiotes plant powder with a modifier for modification, and drying to obtain modified pistia stratiotes; step 2, carrying out high-temperature cracking reaction on the modified pistia stratiotes in an anaerobic or anoxic state in a protective atmosphere to obtain pistia stratiotes charcoal; and step 3, mixing the pistia stratiotes charcoal with an acid-base regulator, rinsing until the pistia stratiotes charcoal is neutral, and drying to obtain the adsorbent. According to the adsorbent and the preparation method and application thereof, the technical defects that an existing adsorbent is weak in adsorption capacity, particularly weak in adsorption capacity for ammonium nitrogen in a polluted water body, incapable of meeting the emission standard and incapable of meeting the requirements of people can be effectively overcome.

The invention discloses an aquatic plant in-situ restoration method of a eutrophicated water body. In the subtropical monsoon moist climate region, when the ambient temperature exceeds 10 DEG C, the restoration plant Pistia is put into the eutrophicated water body and subjected to float planting; by the aid of the riverbank or existing dike of the water body, a bamboo pole fence is adopted to prevent the Pistia from diffuse randomly; the Pistia enriches nitrogen and phosphorus in the water body in the growth process; and the Pistia is periodically salved to indirectly remove the nitrogen and phosphorus eutrophicated substances in the water body, and the salved Pistia plant is recycled. The method has the advantages of obvious nitrogen and phosphorus removal effects; and the salved Pistia plant can be recycled and used as a biomass energy material for biogas fermentation, a raw material for rotting farmyard manure and a raw material for livestock/poultry cultivation feed.

The invention provides traditional Chinese medicine for treating cervical cancer and a preparation method. Raw herbal materials of the traditional Chinese medicine comprise gynura bicolor, pistia stratiotes, edgeworthia chrysantha, hylocereus undatus, timothy grass, argemone mexicana, artemisia sphaerocephala, tamarix chinensis, tropic ageratum herbs, alder bark, corydalis balansae, manchurian beakgrain, oyster plants, licorice sections, roxburgh roses, franchet peashrub flower roots, pungent litse root bark and sassafras tzumu. The traditional Chinese medicine has the advantages that the exact curative effect on the cervical cancer is achieved, toxic and side effects are few, relapsing is not prone to occurring, and the survival time of cervical cancer patients can be significantly prolonged.

The invention discloses an artemisia apiacea hundred-herb-medicine oil and a preparation method and application thereof, and aims to solve the problem that existing mosquito repellent products are poor in using effect. The artemisia apiacea hundred-herb-medicine oil comprises medicinal components and plant oil, and the medicinal components comprise artemisia apiacea leaves, wild chrysanthemum flowers, honeysuckles, flos carthami, flos magnoliae liliflorae, lonicera japonica, paniculate swallowwort roots, radix isatidis, dandelions, radix zanthoxyli, murraya paniculata, pistia stratiotes, selaginella tamariscina, abrus mollis, groundsel, schizophragma integrifolium, wormwood, vitex negundo L leaves, vitex negundo L seeds, litsea cubeba, fructus cnidii, rhizoma acori graminei, melaleuca alternifolia, fructus amomi, angelica pubescens, notopterygium, cassia twigs, agastache rugosus, clove, radix angelicae, lalang grass rhizome, ligusticum wallichii, pseudo-ginseng, artemisia apiacea oil,artemisinin, sandalwood oil, agilawood oil, dalbergia wood oil, camphor oil, peppermint oil, methyl salicylate, turpentine, eucalyptus oil and verbena oil. The hundred-herb-medicine oil prepared through plant oil extraction and steam distillation preparation technologies using the principle that like dissolves like is fragrant and pleasant in smell, wide in applicable people range and convenient to use.

The invention relates to the field of plant molecular biology, in particular to an EjAG gene determined by stamen and pistil characteristics of loquat, protein encoded by the EjAG gene and application. The whole length of the cDNA (complementary deoxyribonucleic acid) sequence of the EjAG gene is shown in SEQ ID No.1; the amino acid sequence of the protein encoded by the EjAG gene is shown in SEQID No.2. The EjAG gene is only expressed in the stamen and pistil of the loquat, but not expressed in the leaves, sepal and petals. An expression carrier pBI121-EjAg is guided into an arabidopsis ag-1mutant by an agrobacterium-mediated bud infection method; when the transgenic arabidopsis ag-1 mutant flowers, the number of petals and the number of rings are reduced, and the stamen and pistil arerestored. The EjAG gene has the advantages that the theoretical foundation is laid for the modification of plant flower organs by the EjAG gene of the loquat, and the technical scheme for the modification of plant flower organs by the EjAG gene of the loquat is provided; the EjAG gene of the loquat is over-expressed in the arabidopsis ag-1 mutant, so that the phenotype of double flower is restoredinto the normal phenotype; the application prospect is good.

The invention discloses a kind of screening method for barley re-cadmium-poison genotype, comprises selecting seed, disinfecting, culturing seedling, transplanting, pre-culturing, cadmium coerion treatment, re-cadmium-poison evaluation; cadmium treat 20 days, detect value of the chlorophyll device for the middle of the functional leave; the decline ratio(%)= 100X(20Umcd treatment detection value - concrete with detection value)/ concrete with detection value ; the integration score of each breed= value of the chlorophyll device declined ratioX0.5+ root volume declined ratioX0.1+root number declined ratioX0.1+ dry weight of underground declined ratioX0.1+stalk high declined ratioX0.1.

The invention discloses a method for promoting radish pollination through carbon dioxide and honey. The method includes the following steps of S1, planting radishes in a plant culture medium, conducting vernalization treatment for three months on radish plants in the radish seed production stage, and keeping the night temperature at 1-5 DEG C to complete vernalization; S2, making the small vernalized radishes naturally grow for 2-3 months in the plant culture medium into meat radishes; S3, transplanting the meat radishes into a large field, watering the meat radishes in time after transplanting, and installing insect prevention nets on vent holes in the two sides of a greenhouse and a worker entrance and exit after transplanting and before flowering. By conducting artificial intervention through carbon dioxide, the incompatibility between pollens and pistil tissue can be broken, and therefore the hybridization aim is realized; when radish seeds are cultured, the critical growth periodis short, labor and land are saved, the radish seeds are suitable for being densely planted, the seed yield of unit area is high, the pollination rate is effectively increased through bees, the seed production cost is reduced, and the hybrid seed purity is high.

The invention discloses an artificial pollination method of eucalyptus. The artificial pollination method comprises the following steps: (1) selecting seed trees: selecting the seed trees with excellent heredity quality and high combining ability; (2) performing castration: selecting flower branches with moderate flowering amount and moderate maturity, wherein the quantities of buds is 20 or above, and the moderate maturity means opercula is light white and soon drops optimally; (3) performing bagging: performing isolation on branches to be subjected to pollination so as to prevent pollution of non-target, pollen; (4) performing pollination: mastering good pollination opportunity during the pollination, when top ends of gynoecium stigmas expand and secrete certain mucus, performing pollination, wherein the optimal pollination period is the time 3-5 days after castration, and during the pollination operation, performing pollination from bottom to top so as to avoid the condition that the stigmas are damaged after being hit. Compared with the prior art, the artificial pollination method disclosed by the invention has the advantages that artificial pollination is an important means for performing hybridization breeding and cultivating new species of the eucalyptus; according to the artificial pollination method disclosed by the invention, the operation is convenient, the dosage of the pollen can be saved, the success rate of the pollination is increased, the fruit setting rate is increased, and the final effective hybrid seed number is increased.

A fringe flowered Madagascar periwinkle, especially a long blooming Madagascar periwinkle whose pistil does not have pollination ability is provided and a method for producing fringe flowered long blooming Madagascar periwinkle is provided as a purpose. A fringe flowered Madagascar periwinkle was bred by pollinating a pollen of a fringe flowered Madagascar periwinkle comprising a sterile pistil to a commercial variety. Moreover, by a self pollination of the fringe flowered Madagascar periwinkle a fringe flowered and pistil sterile Madagascar periwinkle is obtained.

The invention discloses a method for bagging-free cross pollination of a cherry blossom. The method comprises the first step of arranging a centrifugal tube orifice for filling pollen close to a stigma, dipping the pollen with an eye liner brush and applying the pollen on the surface of the pistil stigma gently till the surface of the stigma is covered with pollen; the second step of setting pollination time: completing pollination in time after emasculation in the morning from 8:00 to 10:00 or in the afternoon from 4:00 to 6:00; the third step of scaffold erection: erecting a support frame onthe periphery of a stock tree after the pollination of the whole stock tree; the fourth step of insect-proof net covering: covering a 40-60 mesh insect-proof net on a support frame. A pollen storagemethod is provided, and solves the problem that a male parent flowering period is earlier than a female parent flowering period in the problem that the male parent flowering period and the female parent flowering period do not meet in the cross pollination. The maturing rate of the cross pollination is improved by reasonable pollination methods and steps. Due to the fact that the support frame iserected on the periphery of the stock tree after the pollination, and the insect-proof net covers the tree, the process of bagging and bag removing is eliminated, the workload of the cross pollinationis greatly reduced, and the working efficiency of the cross pollination is improved. Birds are prevented from eating hybrid fruit.

The invention discloses quick-preparation type plant alkaloid insecticidal powder for peach trees and a preparation method of the quick-preparation type plant alkaloid insecticidal powder. the plant alkaloid insecticidal powder is prepared from the following components in parts by weight: 4.534-8.655 parts of 1,2-dicarboxy-propyl carborane, 3.982-9.376 parts of N-bromosuccinimide, 9.456-14.269 parts of cod-liver oil extracts, 4.257-8.657 parts of methyl methacrylate, 1.354-4.156 parts of biscyclopentadienyl iron, 5.698-7.156 parts of acetic p-toluenesulfonic anhydride, 4.598-7.325 parts of divinyltetramethyldisiloxane, 18.657-20.723 parts of alliin, 10.687-15.893 parts of divinyltetramethyldisiloxane, 12.586-17.951 parts of calla extract liquor, 7.635-12.675 parts of methyl trifluoromethanesulfonate, 1.589-7.896 parts of N, N-dimethylformamide, 10.893-16.753 parts of 2-oxo-1-imidazolidinecarbonyl chloride, 8.394-12.756 parts of 3-acetyl-1-chlorocarbonyl-2-imidazolidone, 14.659-18.546 parts of 3-chlorocarbonyl-1-methanesulfonyl-2-imidazolidinone, 4.893-8.885 parts of 1-(2-hydroxyethyl)-2-imidazolidinone, 6.786-10.563 parts of 1-methylsulphonyl-2-imidazolidone, 15.689-22.486 parts of 1,3-dimethyl-2-imidazolidinone, 17.986-22.258 parts of 1-acetyl-2-imidazolidinone and the like and 5.698-8.467 parts of pistia stratiotes extract liquor.