Method for performing artificial crossbreeding without removing stamen from catalpa bungei and application thereof

A technology of artificial hybridization and catalpa, which is applied in the fields of application, botany equipment and methods, plant gene improvement, etc., can solve the problems of catalpa self-fertilization, catalpa not emasculation, inability to pollinate and bear fruit, etc., to reduce labor costs Strength, guaranteed efficiency effect

Inactive Publication Date: 2012-10-10
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problem that Chinese catalpa is sterile, lacks pollinators, and cannot be pollinated in a natural state, the purpose of the present invention is to provide a method for catalpa without emasculation and artificial cross breeding

Method used

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  • Method for performing artificial crossbreeding without removing stamen from catalpa bungei and application thereof
  • Method for performing artificial crossbreeding without removing stamen from catalpa bungei and application thereof
  • Method for performing artificial crossbreeding without removing stamen from catalpa bungei and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] In 2010, 3 individual plants of the catalpa test forest in Yanshi City, Henan Province were selected as female parents, and 2 individual plants were used as male parents for artificial pollination, which specifically included the following steps:

[0022] 1 pollen collection

[0023] Catalpa trees in Yanshi generally bloom after April 20. However, due to the low temperature in the spring of 2010, the flowering time was delayed by 1 week. After the catalpa blossomed on May 1, the development and changes of the anthers were observed in time. From May 5 to 6, when the anthers were swollen, the surface was milky white, and had a shiny luster, but the anthers were not cracked, the anthers were collected. Gently pick off the anthers from the stamens with medical tweezers, and place them in a clean glass petri dish (the size of the petri dish is selected according to the amount of pollen collected), avoiding direct sunlight. Then put the pollen in an air-conditioned room to ...

Embodiment 2

[0031] In 2009, 4 individual plants of the catalpa test forest in Tianshui City, Gansu Province were selected as female parents, and 4 individual plants were used as male parents for artificial pollination, which specifically included the following steps:

[0032] 1 Pollen Collection and Preservation

[0033] In Tianshui City, Catalpa generally blooms after May 5th. After the Catalpa blooms on May 7th, observe the development and changes of anthers in time. Collect anthers when they are not dehiscated. Gently pick off the anthers from the stamens with medical tweezers, and place them in a clean glass petri dish (the size of the petri dish is selected according to the amount of pollen collected), avoiding direct sunlight. Then put the pollen in an air-conditioned room to dry in the shade, and the room temperature is controlled at 20-22°C. After drying in the shade, the pollen was stored in a refrigerator at 4°C.

[0034] 2 artificial pollination

[0035] On May 12, the weat...

Embodiment 3

[0041] In 2011, 4 individual plants of the catalpa test forest in Tianshui City, Gansu Province were selected as female parents, and 3 individual plants were used as male parents for artificial pollination, which specifically included the following steps:

[0042] 1 Pollen Collection and Preservation

[0043] In Tianshui City, catalpa usually blooms after May 5th. After the catalpa blooms on May 7th, observe the development and changes of anthers in time. When the anthers are swollen from May 10th to 11th, the surface is milky white and has a shiny luster. , but not dehisced for anther collection. Gently pick off the anthers from the stamens with medical tweezers, and place them in a clean glass petri dish (the size of the petri dish is selected according to the amount of pollen collected), avoiding direct sunlight. Then put the pollen in an air-conditioned room to dry in the shade, and the room temperature is controlled at 20-22°C. After drying in the shade, the pollen was ...

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Abstract

The invention provides a method for performing artificial crossbreeding without removing stamen from catalpa bungei. The method particularly comprises the following steps: (1) collecting pollen: collecting anther before the anther of the catalpa bungei is mature and does not crack, drying the anther indoors at the room temperature of 20 to 22 DEG C and collecting pollen after the anther cracks; and (2) performing artificial pollination: when the stamen of each flower of catalpa bungei is mature, the stigma is opened in a reverse V shape and the surface has milk white luster, lightly touching with hands or shaking catkin and quickly closing to perform artificial pollination. By the method, the artificial pollination is properly performed by selecting the optimum pollination time at the appropriate air temperature and under the clear, windless or breeze condition, so that a seed tree can be fertilized successfully and fructify a large number for fruits, and the requirements of scientific research and production can be met.

Description

technical field [0001] The invention relates to the field of plant breeding, in particular to a plant hybrid breeding technology. Background technique [0002] Catalpa bungei is a deciduous and tall tree belonging to the Bignoniaceae genus Catalpa Scop. It is native to my country and has strong adaptability. It is mainly distributed in Henan, Hubei, Anhui, Shandong, Shanxi and other provinces. , has a long history of cultivation, and is a precious and high-quality timber tree species traditionally cultivated in my country and a famous garden ornamental tree species. However, there are very few natural wild plants. Within its distribution range, except for a small number of natural forests with multiple generations of germination in a few places, most of them are artificially cultivated "surrounding" trees. Catalpa is an insect-borne flower, autoflowering is sterile, probably due to the lack of pollinators, it is difficult to pollinate and bear fruit in a natural state. [0...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 王军辉
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY
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