115 results about "Catalpa bungei" patented technology

The invention relates to a method for increasing the tender bud cutting and rooting rate of catalpa bungei in a mode of removing terminal buds of cutting slips. The method is characterized by comprising the following steps: selecting three-year to four-year strong branches of catalpa bungei in March, and cutting into 30-40cm shoot segments for sand storage; when the height of newly-germinated tender buds in a sand bed reaches 10-15cm, removing the terminals of the tender buds; after ten days, taking down the tender buds with heels as cutting slips; after cutting off half of leaves on the cutting slips, soaking roots into 75% alcohol for disinfection for 30 seconds, then soaking into 3000mg.L<-1> IBA (Indole Butyric Acid) for 60 seconds, and finally inserting the cutting slips into a seedling-raising disk; inserting one cutting slip into each hole cup with the depth to be 1 / 2-1 / 3 of the length of the cutting slip; after cutting, using a 85% shade net to cover for sun block, and using an automatic spray device to spray for supplying water; and hardening seedlings for 10-15 days and then planting after entering the seedling hardening period, namely 70 days after cutting. The survival rate of catalpa bungei tender buds cut by the method can reach more than 80 percent, and the popularization and application of catalpa bungei are greatly promoted.

The invention discloses a tissue culture method for increasing the multiplication coefficient of catalpa bungei. The tissue culture method comprises five steps of building sterile culture, inducing axillary bud, performing multiplication of the axillary bud, performing rooting culture of bud seedling and performing tissue culture of seedling. The method is used for culturing tissue and quickly culturing seedling of the catalpa bungei; the method has the advantages of high bud induction rate, large multiplication coefficient, high rooting rate and high transplanting survival rate. By virtue of the method, an optimal culture medium formula of the catalpa bungei is selected and prepared by 1.5mg / L of MS+BA, 0.1mg / L of ZT and 0.2mg / L of IAA, and a subculture method for subculturing stem and root callus during subculturing is also selected; the multiplication coefficient of catalpa bungei is 11.0-23.2, and the average multiplication coefficient is 15.3. By virtue of the method, a seedling culturing method good in multiplication speed, short in production cycle and good in stability is provided for large-scale cultivation of the catalpa bungei in the forestry production.

The invention discloses a cuttage propagation method for survived seedlings by tissue culture and transplantation of catalpa bungei, and is characterized by comprising the following steps of: 1), preparing a cuttage substrate and a root growth promoting agent; 2) scissoring 30 to 50cm of twigs when the survived seedlings obtained through the tissue culture and transplantation of the selected good singleplant tissue grow to a height between 50 and 80cm, shearing the twigs into 5 to 15cm of small segments and reserving 1 / 3 blades, dipping the small segments into the prepared root growth promoting agent for 5 to 8 s, and inserting the dipped small segments into the prepared substrate; and 3) inserting cuttingwood into a potted tray containing the substrate, then totally sealing the potted tray with a plastic film, and keeping the relative humidity between 85 and 95 percent, and the temperature between 20 and 30 DEG C, wherein a cuttingwood base starts generating callus after 10 to 13 days, the callus grows a root system after 18 to 21 days, the root system grows buds after 25 to 30 days, and the seedlings are transplanted after 40 days. The method can keep the original good properties of a catalpa bungei stock plant, and has over 95 percent of cuttage survival rate, low cost and high efficiency.

A cultivation model of Catalp asexual propagation uses a model which includes two steps: first, makes Catalp asexual stem quick dipping in a solution of IBA, NBA (2:1) 500-1500ppm; second, Catalp asexual stem which is dipped in the solution is levelly buried in the middle to grow in a soil substrate containing vermiculite, perlite and sand. The cultivation model has a plurality of advantages: the cultivation model is simple and easy to learn for forestry and agriculture; degradation phenomenon of good characters for seeds propagation is avoided by propagating Catalp chozen clones using the cultivation model; Catalp seedlings can be propagated massively and quickly; moreover, the average survival rate reaches to more than 95%, and the average survival rate of conventional cuttage method is between 10% and 20%; the raw material used is cheap and easy to be get; the method is simple and practicable. The invention provides a new model for Catalp chozen clones widespreading and seeding fast-obtaining in the process of fast-growing plantation in our country.

The invention discloses a Chinese catalpa tree fast asexual propagation method by branch cottage comprising a bud sprout preparation stage, a root speeding stage, a management stage and a transplanting stage after cottage, wherein the bud sprout preparation stage includes seed strip and seed root handling, soil working, seed strip laying and management process, the root speeding stage includes a nursery sock growing bed preparation and bud collection cottage procedure. The invention realizes fast growth rate and high survival rate.

An in-vitro culture method for catalpa bungei comprises 1) inducing callus, concretely, putting catalpa bungei on a callus induction medium for inducing callus, wherein the callus induction medium is obtained by adding 6-benzyladenine (6-BA) and 2,4-dichlorophenoxyacetic acid (2,4-D) on the basis of 1 / 2 MS basal medium; 2) propagating callus, concretely putting callus on a propagation medium for propagation, wherein the propagation medium is obtained by adding 6-benzyladenine (6-BA) and alpha-naphthalene acetic acid (NAA) on the basis of 1 / 2 MS basal medium; 3) inducing embryonic callus and differentiating somatic embryo, concretely putting propagated callus on an induction differentiation medium, wherein the induction differentiation medium is obtained by adding 6-benzyladenine (6-BA) and alpha-naphthalene acetic acid (NAA) on the basis of 1 / 2 MS basal medium; and 4) regenerating a plant, concretely putting somatic embryo obtained through differentiation on a regeneration medium for inducing somatic embryo to germinate, so as to obtain a catalpa bungei regeneration plant. The method aims at expanding catalpa bungei propagation coefficient and providing conditions for germplasm preservation and genetic transformation.

The invention discloses a method for improving the propagating efficiency of catalpa bungei grafting rootstocks and belongs to the field of propagation of elite tree species. In the process of catalpa bungei grafting during rootstock cultivation with sowing and seedling raising, part of seedlings cut off from the upper part of a catalpa seedling for producing the rootstock carries out cutting propagation, and the seedlings can be used for grafting the rootstocks after one year; in other words, besides the first-time rootstock production with sowing and seedling raising, the rootstocks are obtained by raising seedlings by cutting of part of seedlings cut off from the upper part of a rootstock block, and the method is circularly applied to the grafted propagating of catalpa bungei. The cycle of raising seedlings by cutting achieving the condition of producing the grafted rootstocks is one year, and the regular two-year cycle of sowing and seedling raising is shortened by one year, so that the seedling raising efficiency is greatly improved, and the rate of survival of seedling raising is greatly increased. The cut off catalpa stems after producing the grafted catalpa rootstocks are fully utilized, so that the resource waste is avoided, and the seedling raising cost of rootstock production is reduced. Therefore,, the quick rootstock cultivation is realized, and the propagating efficiency of catalpa bungei grafting is greatly improved.

The invention discloses a rooting culture method of catalpa bungei tissue culture seedling. The method sequentially comprises the following steps: (1) obtaining aseptic seedlings of the catalpa bungei; (2) subculturing the aseptic seedlings; (3) performing rooting culture of the catalpa bungei tissue culture seedlings; (4) hardening seedlings and transplanting. By adopting the catalpa bungei tissue culture seedling rooting culture method, the propagation ratio is controlled to 1: (3-3.5), the height of the plant of the seedlings is controlled to be 2.5-4cm, and the rooting culture is carried out to guarantee that the rooting proportion is up to 90 percent or above; the production cost is reduced, and the same culture medium is used in the steps of obtaining the aseptic seedlings and subculturing during the rooting culture of the catalpa bungei tissue culture seedling, so that an operation step is eliminated and the production cost is reduced, thus being more suitable for industrial production. According to the rooting culture method of the catalpa bungei tissue culture seedling, the forest tissue culture industrialization for an experiment is realized, the industrialization process is simplified, the steps are reduced, the labor force is controlled, and the cost control is facilitated, thus promoting the industrialization of the woody plant tissue culture. The method is suitable for the rooting culture of the catalpa bungei tissue culture seedling.

The invention discloses a novel method for improving catalpa bungei cuttage propagation efficiency.The method includes the first step of construction of a cutting orchard, the second step of preparation of a cuttage seedbed, the third step of preparation of a cuttage matrix, the fourth step of cultivation and harvesting of cuttings, the fifth step of cuttage, the sixth step of daily management after cuttage, and the seventh step of seedling hardening and transplanting.According to the transpiration and rooting rules of plants, tender branches low in lignification degree are obtained in a shading mode, cuttings are subjected to shading treatment through a shading method, the catalpa bungei cuttage survival rate is greatly increased and as high as 92%, inconvenience caused by catalpa bungei seedling propagation by means of tissue culture, grafting and other technologies is overcome, nursery cultivation time is greatly shortened, operation is easy, and the method is convenient to control and good in practicality.

The invention provides a method for generating somatic embryos of catalpa bungei through induction. The method comprises the steps of after matured catalpa bungei seeds are disinfected, putting the catalpa bungei seeds into a culture medium for callus induction, generating callus tissues through dark cultivation, then performing subculture multiplication cultivation on the callus tissues to obtain a large amount of calluses, and finally paving the calluses into an induction culture medium to generate the somatic embryos of the catalpa bungei through the induction. A mature regeneration system for cultivating a new insect and disease-resistant product of the catalpa bungei by a transgenic technology is supplied.

The invention provides a cultivation method for micropropagation of catalpa bungei. The cultivation method comprises a step 1) of expalnt obtaining; a step 2) of change over culture; a step 3) of multiplication of subculture; a step 4) of cuttage culture; and a step 5) of seedling exercising and transplantation. A propagation material for the cultivation method for micropropagation of catalpa bungei comes from a matrix, namely a bred excellent seed tree, it is guaranteed that produced seedlings are all good stocks representing female parent clone, a phenomenon of variety complexity does not exist, a seedling culture propagation coefficient is remarkably improved, and the rooting rate, and the survival rate and the strong seedling rate of cutting seedlings are improved. Besides, the tissue culture rooting process of the catalpa bungei tissue culture seedlings is omitted, rootless seedlings with heights of 2-3 m are directly utilized to perform micro cuttage culture, and culture time and production cost are greatly saved while the seedling expanding propagation coefficient is ensured.

The invention discloses a tissue culture and rapid propagation method and device for catalpa bungei and relates to the technical field of agriculture. The device comprises a carbon dioxide generator,culture boxes and an LED lamp tube, wherein a valve for controlling flow velocity of carbon dioxide is arranged at an outlet of the carbon dioxide generator, and the carbon dioxide generator is used for producing, storing and releasing carbon dioxide gas; sugar-free media are arranged in the culture boxes, and the culture boxes are used for inoculated culture of catalpa bungei tissue culture seedlings; an inlet of the valve is connected with an outlet of the carbon dioxide generator, and an outlet of the valve is communicated with gas inlets of the culture boxes through pipes; the LED lamp tube is arranged above the culture boxes and used for irradiating the culture boxes and providing light source for photosynthesis of the catalpa bungei tissue culture seedlings. According to the method,the sugar-free media are adopted for culturing the catalpa bungei tissue culture seedlings, carbon dioxide is adopted to replace sugar to serve as a carbon source, and autotrophic growth of the catalpa bungei tissue culture seedlings is realized through photosynthesis. The method has quite low contamination rate, and the sugar-free tissue culture seedlings are higher in survival rate and grow well.

The invention discloses a method for improving the reproduction efficiency of catalpa bungei grafted seedlings and relates to the field of forest elite reproduction. The method comprises the steps that firstly, catalpa ovata grafting stocks are cultivated through the sowing and seedling raising mode, catalpa bungei grafting is conducted by bud grafting annual catalpa ovata stocks provided with xylem, after the grafting, twig cuttage is conducted on remaining catalpa ovata stocks, so that grafting-used stocks are cultivated, and the catalpa ovata stocks can be used as catalpa bungei bud-grafting stocks in June of the next year. Compared with a conventional method of cultivating stocks through sowing and seedling raising, the cycle is shortened by one year, by means of the method of reproducing the catalpa ovata stocks through cuttage simultaneously, catalpa ovata stem segments which are cut away in the grafting process are utilized sufficiently, resource waste is avoided, meanwhile, the seedling raising cost is reduced, the seedling raising efficiency is improved, the catalpa ovata stocks can be circularly applied to catalpa bungei grafting and reproduction in the future, rapid cultivation and efficient utilization of the stocks are achieved, the catalpa bungei grafting and reproduction efficiency is greatly improved, the seedling raising cycle is short, and the survival rate is high.

The invention relates to the technical field of vegetative propagation and cultivation of seedlings, and particularly relates to a catalpa bungei rapid cuttage propagation method. The method comprisesthe following steps that annual robust seed branches of catalpa bungei variety and clone are selected to be disinfected and then placed in a pregermination pond to grow cutting slips, coppice shootsare obtained after 5-7 weeks, and disinfection is conducted after the cutting slips obtained is pruned; peat and perlite of 2:1 are selected as cutting medium materials, the peat and perlite are inserted into a point disk, 1L of talcum powder are mixed with ABT No.1 500*(10-6)mg / L and GGR No.7 (80*10)-6mg / L to prepare rooting powder, the base portions of the cutting slips are evenly dipped into the rooting powder and put into the holes formed in the point disk, and the cutting slips are poured over with clean water and sprayed with bactericide after fixed; atomization management is performed within 1-10 days after cuttage, the atomization management is stopped after 10 days, illumination is increased, ventilation is conducted, bactericide is sprayed at regular intervals, and foliar fertilizers are sprayed at regular intervals after 3 weeks of the cuttage to obtain cuttage seedlings; 5 weeks after the cuttage, full illumination is supplied, temperature is controlled, regular watering isperformed, fertilizers are applied, and hardening-seedling is conducted; 7-8 weeks after the cuttage, the cuttage seedings are transplanted, sufficient water is poured over on the cuttage seedlings,and then managements such as watering, fertilization and the like are performed. The method has the advantages of high rooting rate, good universality and low cost.

The invention provides a method for performing artificial crossbreeding without removing stamen from catalpa bungei. The method particularly comprises the following steps: (1) collecting pollen: collecting anther before the anther of the catalpa bungei is mature and does not crack, drying the anther indoors at the room temperature of 20 to 22 DEG C and collecting pollen after the anther cracks; and (2) performing artificial pollination: when the stamen of each flower of catalpa bungei is mature, the stigma is opened in a reverse V shape and the surface has milk white luster, lightly touching with hands or shaking catkin and quickly closing to perform artificial pollination. By the method, the artificial pollination is properly performed by selecting the optimum pollination time at the appropriate air temperature and under the clear, windless or breeze condition, so that a seed tree can be fertilized successfully and fructify a large number for fruits, and the requirements of scientific research and production can be met.

The invention discloses a rapid breeding method for tissue culture seedlings of catalpa bungei. The method comprises the following steps: building a winter-warming sunlight greenhouse seeding-raising shed, building seedling-raising ponds, building an atomizing automatic control system, processing bottles for tissue culture seedlings of catalpa bungei, inserting bottles for the tissue culture seedlings of catalpa bungei into the seeding-raising ponds for seedling-raising management, planting tissue culture seedlings of the catalpa bungei hardening seedlings to harden the tissue culture seedlings in nutrition pots, and planting grown seedlings on a large field. The method helps resolve problems such as long culture time and high culture cost. The overall seedling raising process takes 30-35 days including culture of the tissue culture seedlings in the seedling-raising ponds and seedling hardening in a transition shed. The commonly-used method of rooting and hardening the tissue culture seedlings of catalpa bungee takes 120-150 days. By contrast, the duration is greatly shortened. The survival rate of seedling breeding is up to over 95%. In terms of the survival rate, the rapid breeding method for the tissue culture seedlings of catalpa bungee has a great improvement.

The invention relates to a three-stage matched cutting cultivation method for catalpa bungei and relates to an asexual breeding technical mode in forestry science. The method comprises the following steps of (1) establishing a first-stage rapid breeding system, collecting branches which grow out in the current year for sand storage at the end of February, adopting small seedlings of catalpa treesas stocks after germination, and carrying out bud grafting on base parts with xylem to obtain grafted seedlings; (2) establishing a second-stage rapid breeding system, constructing a temporary cuttingorchard, planting the grafted seedlings obtained in step (1), and adopting a burying stem germination acceleration cuttage mode disclosed in the publication number CN107996166A or adopting a full-illumination mist spray cuttage mode or adopting a hard branch cuttage mode to obtain cutting seedlings; (3) establishing a third-stage rapid breeding system, planting the cutting seedlings obtained in step (2), establishing a cutting orchard of own-rooted trees, and repeating the cuttage modes in step (2) or adopting root sections for cuttage, wherein the harvesting deadline of the cutting orchard of the own-rooted trees is 5 years, rejuvenation is required five years later for harvesting for another 4 years, and steps (1), (2) and (3) are repeated after 4-year rejuvenation. According to the method, a three-stage rapid breeding system is established, so that the breeding coefficient is greatly increased, and the cuttage survival rate and transplanting survival rate of catalpa bungee are increased.

The invention discloses a method for regenerating plants by high-frequency induction of leaves of catalpa bungei. The method comprises the steps of primary culture, subculture, adventitious bud induction, strong seedling rooting culture and the like. An adventitious bud induction culture medium comprises an improved N6 basic culture medium, 2.5 mg / L of 6-BA, 0.01 mg / L of IBA, 0.1 mg / L of TDZ, 30 g / L of sucrose and 7.0 g / L of agar. The method is simple and convenient to operate, rapid amplification of tissue culture seedlings of the catalpa bungei can be realized in a short time, technical support is provided for excellent planting resource protection and large-scale rapid production of the catalpa bungei, meanwhile, a foundation is laid for genetic engineering breeding of the catalpa bungei, and the method has very important significance for development of molecular mechanism research and germplasm resource improvement of the catalpa bungei.

The invention belongs to the technical field of traditional Chinese medicines, and specifically discloses an external traditional Chinese medicine for burns and scalds as well as a preparation method thereof. The external traditional Chinese medicine for burns and scalds is prepared from the following raw material medicines in proportion by weight: 15-25g of garden burnet, 10-18g of scutellaria baicalensis, 12-16g of radix angelicae, 16-22g of asarum, 11-17g of rhizoma corydalis, 25-35g of catalpa bungei flower, 8-16g of polygonum cuspidatum, 5-15g of borneol, 12-24g of medicinal cyathula root, 3-12g of rheum officinale, 18-25g of honeysuckle, 10-20g of fructus forsythiae, 6-13g of safflower carthamus, 8-15g of lithospermum, 6-18g of myrrh, 12-18g of rhizoma smilacis glabrae, 15-25g of willow branches, 7-13g of tung tree flower, 9-12g of cacumen biotae leaves and 18-24g of viola. The external traditional Chinese medicine for burns and scalds provided by the invention is reasonable in formula and exact in curative effect; long-term clinic treatment effects prove that the external traditional Chinese medicine has a great curative effect on I-degree, II-degree and III-degree burns and scalds, and can further suppress the hyperplasia of wound surfaces and avoid the residues of hyperplastic scars.

The invention discloses a tissue culture method for improving the multiplication coefficient of catalpa bungei. The method comprises the following steps: obtaining a primary aseptic seedling through primary culture, cutting the seedling into 1.5 to 2.0 cm stem segments with buds, inoculating the stem segments with buds into a secondary culture medium to perform micropropagation multiplication, obtaining cluster buds through callus differentiation, and further performing root induction, so as to obtain plantlets of the catalpa bungei, wherein the formula of the secondary culture medium is as follows: 0.1 to 0.2 mg / L NAA, 0.8 to 0.9 mg / L 6-BA, 30 g / L saccharose and 7 g / L agar are added into N6 culture medium, and the pH value is adjusted to be 5.8 to 6.0. The method has the advantages that callus induction is promoted on the basis that axillary bud proliferation is promoted, and differentiation of cluster buds is promoted on the callus basis, so that more buds can be obtained within thesame growth period, and a new approach is developed for the fine breeding of the catalpa bungei.

The invention discloses nutritional porridge and a preparation method thereof, and belongs to the field of food processing. The nutritional porridge consists of the following components in parts by weight: 35-50 parts of rice, 15-25 parts of soybeans, 20-25 parts of millets, 10-15 parts of yams, 5-10 parts of shrimp meat, 5-15 parts of pork leans, 3-5 parts of shredded gingers, 3-7 parts of honeysuckle, 8-15 parts of celeries, 5-7 parts of carrots, 3-5 parts of edible salt, 1-4 parts of hickory peels, 1-3 parts of catalpa bungei root powder, 15-25 parts of potato powder and 300-450 parts of water. The nutritional porridge disclosed by the invention is balanced in nutrition and is reasonably mixed, so that the effects supplement each other and can be well achieved; furthermore, absorption of calcium can be promoted; cholesterol is reduced, vitamins are supplemented, immunity is enhanced, senilefracture can be prevented favorably, and the nutritional porridge is particularly suitable for middle aged and elderly people.

The invention relates to a bud cutting raising method of Catalpa bungei seedlings, comprising the steps of treating raising substrate, to be specific, selecting moist breathable raising substrate foruse; treating scions, to be specific, after trees of Catalpa bungei bud in early spring, collecting new buds of Catalpa bungei as scions, and using a rooting promoter to promote rooting; performing cuttage, to be specific, subjecting the scions that are subjected to rooting promotion to cuttage, with bud gaps of the scions facing up; raising seedlings, to be specific, irrigating the inserted scions once with root-fixing water immediately, and regularly irrigating the scions to maintain moisture until the scions root and grow into Catalpa bungei seedlings; exercising the seedlings, to be specific, after the Catalpa bungei seedlings root, raising the ambient temperature gradually, cultivating in the open field, and transplanting after the Catalpa bungei seedlings grow stably. The bud cuttingraising method of Catalpa bungei seedlings provided herein is simple to perform, provides significantly shortened raising cycle of Catalpa bungei seedlings and provides increased survival rate of seedlings per mu; in addition, the bud cutting raising method of Catalpa bungei seedlings has no strict requirements on a seedling raising environment, allows seedling raising cost to be greatly reduced,and is significant to the reduction of seedling raising cost for Catalpa bungei and the improvement of breeding efficiency for Catalpa bungei.

The invention belongs to the technical field of plant tissue culture, and particularly relates to a tissue culture method for increasing the differentiation number of effective new shoots of large-leaf catalpa bungei. According to the method, a set of combined culture mediums is utilized for increasing the differentiation number of the effective new shoots of large-leaf catalpa bungei, wherein thecombined culture mediums include the induction culture medium, the differentiation culture medium and the growth culture medium. The tissue culture method for increasing the differentiation number ofthe effective new shoots of large-leaf catalpa bungei comprises the steps of establishment of a sterile system, subculture in a two-step relay mode, rooting induction culture, seedling hardening, transplanting and the like. By means of the tissue culture method for large-leaf catalpa bungei, on one hand, the components of the culture mediums are optimized and adjusted; on the other hand, the culture mode is adjusted in the subculture process, tissue culture seedlings are subjected to subculture in the specific culture medium in the two-step relay mode, the total culture time is not prolonged,the proliferation and growth process is considered at the same time, the number of the effective new shoots is increased, and meanwhile the vitrification phenomenon in the tissue culture process is well avoided.

The invention relates to the field of plant molecular biology, in particular to a CabuAG gene related to the pistil and stamen development of catalpa bungei and encoded protein and application thereof. The CabuAG gene related to the pistil and stamen development of the catalpa bungei belongs to one of the following two kinds of protein, wherein the first kind of the protein is composed of amino acid shown in SEQ ID No.2, and the second kind of the protein is derived from the first kind of the protein and obtained by the fact that the amino acid sequence shown in SEQ ID No.2 is replaced by or lack of or added with one or several amino acids and has the same activity. The invention provides the gene for encoding the protein and application thereof in plant flower organ improvement. The invention further provides an express carrier containing the CabuAG gene. Compared with a wild plant, the CabuAG-transgenic plant can show the characteristics of special development of the flower organs.

The invention relates to a rapid catalpa bungei cultivating method integrating grafting and cuttage. Catalpa ovata branches serve as stocks for heteroplastic grafting, cuttage is performed for seedling raising after catalpa bungei with xylems undergoes bud grafting. The rapid catalpa bungei cultivating method integrating grafting and cuttage is simple in operation, the stock utilization rate is high, the grafting and cuttage are not influenced by time, weather, environment, temperature and the like, the cuttage rotting rate is up to 100%, and the survival rate is up to 98% or above. The problems of slow catalpa bungei seedling breeding, short grafting period, low preserving rate after conventional grafting, long adult seedling cultivating time, low stock commodity and the like are solved, and accordingly the demand for a large number of fine catalpa bungei varieties, stocks and adult seedlings in the market. The seedling raising period can be shortened to be one year by adopting the method, the seedlings are orderly in height, the production cost is greatly reduced, the seedling raising speed is improved, factorized catalpa bungei production is achieved, and the economical benefit is remarkable.

The invention discloses an ex-vitro rooting method for tissue culture seedlings of catalpa bungei. The method comprises the following steps that 1) a rooting material is selected; 2) a wound of the rooting material is healed; 3) the tissue culture seedlings are subjected to cutting rooting; 4) the seedlings are tended in a nutrient block. According to the ex-vitro rooting method for the tissue culture seedlings of the catalpa bungei, firstly, an incision of the rooting material is subjected to wound healing treatment, then later rooting culture is conducted so that the tissue of the incision can be healed well, the risk that the incision is infected with bacteria is reduced, and in the later period, the seedlings can root better; the whole rooting culturing time is shortened, in addition,after rooting, the catalpa bungei seedlings can grow roots in a natural environment at the indoor temperature, the main root has a large number of fibrils, the root system activity is high, after 3-5days of new root growing, new roots can directly absorb nutrition in a medium to promote new leaf growing, and the survival rate of rooting is high. The method is suitable for the tissue culture seedlings of the catalpa bungei.

The invention relates to a method for measuring the malonaldehyde content of catalpa bungei leaves. The method comprises: firstly, carrying out ice bath grinding on collected fresh leaves; then addingtrichloroacetic acid and shaking in a constant temperature shaker to extract malondialdehyde; after an extracting solution is acquired, adding ferric sulfate and a thiobarbituric acid to eliminate soluble sugar interference and carrying out chromogenic reaction; and finally, using a spectrophotometer or a microplate reader to detect the light absorption values of the reaction solution at 450 nm,532 nm, and 600 nm wave lengths, and calculating the malonaldehyde content in the leaves. By using the method for measuring the malonaldehyde content of the catalpa bungei leaves, a problem that themalonaldehyde content of the catalpa bungei leaves can not be rapidly detected is solved; the method is suitable for large scale and simple operation; technical guarantee is provided for the measurement of the malonaldehyde content of the catalpa bungei leaves; and the method is suitable for the evaluation of catalpa bungei germplasm resources, the selection of diverse and appropriate new varieties, the development of a cultivation technology, the measurement of a plant damage degree and other aspects.

The invention provides a catalpa bungei twig cutting propagation method which includes the steps: 1) twig cutting slip acquisition; 2) hormone treatment; 3) twig cutting; 4) seedling hardening and transplanting. According to the catalpa bungei twig cutting propagation method, propagation materials come from a matrix, namely, a bred excellent variety mother tree, all produced seeds and seedlings represent mother clone excellent varieties, variety mixing is avoided, only 25-30 days are needed from cutting to cutting seedling field transplantation, seedling culture time is remarkably improved, the rooting rate, the survival rate and the seedling strengthening rate of cutting seedlings are ensured by controlling a manual cutting environment, and culture time and production cost are greatly saved.