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Tissue culture method for improving multiplication coefficient of catalpa bungei and special culture medium

A technology of proliferation coefficient and tissue culture, which is applied to the tissue culture of catalpa tree, the tissue culture method for improving the multiplication coefficient of catalpa tree and the field of special medium, which can solve the problems of long growth cycle, cumbersome steps, low proliferation rate, etc. The effect of promoting the proliferation of axillary buds with simple steps and great application prospects

Inactive Publication Date: 2019-02-15
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] In order to solve the problems such as cumbersome steps, long growth cycle and low proliferation rate in the production of catalpa, the problem to be solved in the present invention is to provide a tissue culture method and special medium for improving the multiplication coefficient of catalpa

Method used

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  • Tissue culture method for improving multiplication coefficient of catalpa bungei and special culture medium
  • Tissue culture method for improving multiplication coefficient of catalpa bungei and special culture medium
  • Tissue culture method for improving multiplication coefficient of catalpa bungei and special culture medium

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Experimental program
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Effect test

Embodiment 1

[0031] Embodiment 1 improves the tissue culture method of catalpa multiplication coefficient

[0032] (1) Select the stem section or terminal bud of the old Catalpa tree with axillary buds cut to a length of 10 cm, cut off the leaves and keep the petiole of 1.5 cm, soak in detergent for 10 minutes, and then rinse under running water for 20 minutes; On the workbench, soak with 75% alcohol for 30s, then treat with 0.1% mercury liter for 5 minutes, rinse with sterile water for 4 times, cut the petiole to 3mm again to avoid residue, inoculate into the primary culture medium, each bottle The medium is inoculated with 2-3 stem segments, and the first generation is cultured for at least 28 days to obtain the first generation of sterile seedlings;

[0033] Wherein, the formula of the first-generation medium is: MS medium+6-BA 0.5mg / L+IAA 0.15mg / L, the condition of the first-generation culture is aseptic culture at a temperature of 25°C, the light time is 16h / d, and the light intensity...

Embodiment 2

[0037] The test material is "Luqi No. 1".

[0038] (1) Selection of explants: In the experimental nursery of Shandong Provincial Academy of Forestry, cut the young shoots of the old catalpa tree (No. Branch work should be carried out on sunny days.

[0039] (2) Sterilization of explants: cut off the leaves on the tender stems and keep the petioles of 1-2 cm, soak and clean the stems with detergent for 5-10 minutes, and then rinse them under running water. On the ultra-clean workbench, soak in 75% alcohol for 30 seconds, then soak in 0.1% mercury chloride for 5-8 minutes, and then wash with sterile water for 3-4 times. Cut the petiole to 3-5mm again to avoid residue.

[0040] (3) Cultivation of sterile seedlings: cut the sterilized explants into about 1.5cm long stems with axillary buds, and insert them into the primary medium of MS+6-BA0.5mg / L+IAA0.15mg / L 2-3 stem segments were inoculated in each bottle, and the primary culture conditions were aseptic culture at a temperatu...

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Abstract

The invention discloses a tissue culture method for improving the multiplication coefficient of catalpa bungei. The method comprises the following steps: obtaining a primary aseptic seedling through primary culture, cutting the seedling into 1.5 to 2.0 cm stem segments with buds, inoculating the stem segments with buds into a secondary culture medium to perform micropropagation multiplication, obtaining cluster buds through callus differentiation, and further performing root induction, so as to obtain plantlets of the catalpa bungei, wherein the formula of the secondary culture medium is as follows: 0.1 to 0.2 mg / L NAA, 0.8 to 0.9 mg / L 6-BA, 30 g / L saccharose and 7 g / L agar are added into N6 culture medium, and the pH value is adjusted to be 5.8 to 6.0. The method has the advantages that callus induction is promoted on the basis that axillary bud proliferation is promoted, and differentiation of cluster buds is promoted on the callus basis, so that more buds can be obtained within thesame growth period, and a new approach is developed for the fine breeding of the catalpa bungei.

Description

technical field [0001] The invention relates to a tissue culture method of catalpa, in particular to a tissue culture method and a special culture medium for increasing the multiplication coefficient of catalpa, which belong to the field of forest tree breeding. Background technique [0002] Catalpa bungei C.A.Meyer. belongs to the Bignoniaceae family and belongs to the genus Catalpa. It has been cultivated in my country for more than 2,000 years. It is a precious and high-quality timber tree species and a valuable garden ornamental tree species in my country. It has been included in the national timber strategic reserve tree species. Table of contents. Due to the incompatibility of self-pollination, catalpa often blooms and does not bear fruit; even if different clones are mixed together, they can bear fruit after pollination by insects, but there are few seeds, the germination rate of seeds is very low, and seed reproduction is difficult. Catalpa is a tree species that is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 董玉峰姜何燕丽萍王卫东荀守华姜岳忠毛秀红李善文韩丛聪
Owner SHANDONG FOREST SCI RES INST
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