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Preparation method and application of mulberry stem cells

A stem cell and stem cell culture technology, applied in the field of preparation of mulberry stem cells, can solve the problems of long production cycle, high cost, low content of active ingredient 1-DNJ, etc., and achieve the effects of short culture cycle, stable preparation and obvious differentiation

Pending Publication Date: 2022-03-22
北京添易生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the content of active ingredient 1-DNJ in mulberry leaves is low, and the production cycle and cost of extracting the key active ingredient 1-DNJ through mulberry leaf planting are long and high; on the other hand, the total alkaloid and DNJ contents in mulberry leaves will also be affected. Influenced by factors such as variety, collection season and planting base, in addition, the preparation of extracts from mulberry leaves requires control of pesticide residues, so it is necessary to establish a reliable and stable method for preparing mulberry DNJ

Method used

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  • Preparation method and application of mulberry stem cells
  • Preparation method and application of mulberry stem cells
  • Preparation method and application of mulberry stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1, a method of culturing sauna cells using mulberry blades

[0022] This example provides the preparation of mulberry blades for culturing sauna cellular methods including the following steps:

[0023] Step 1, mulberry blankelanter

[0024] The mulberry blanket mulberry exterior was washed with water until there was no residual dirt in the surface, and the mulberry blanket surface was discharged to the surface of the mulberry blanket, and the mulberry sheet was placed in a sterile operator, and the mulberry leaves were cut under sterile conditions. After 6mm × 6mm small piece, after 30 seconds of 75% ethanol solution, the ethanol solution was quickly poured, washed 3 times in sterile water, soak 5 min in the ascorbic acid solution of 100 mg / L, then add 1% bencerens. In a mixed solution of the Leachet and 1% cefphenyl, sterilized treatment for 10 min, then, rinse 5 times with sterile water, dry moisture with sterile filter paper to obtain a smelly mulberry blades. ...

Embodiment 2

[0038] Example 2, a method of preparing sake cell culture using mulberry segments

[0039] Step 1, smectoidal body

[0040] The mulberry exhibitors were washed with water until there was no residue, and the surface moisture was suedened by filtration, and the sterile console was placed in a sterile console for 30 min, then cut the mulberry section into a 6mm small section, with 75 After the% ethanol solution was rinsed for 30 seconds, the ethanol solution was quickly removed, and the sterile water was rinsed 3 times, soak it in an ascorbic acid solution of 80 mg / L in an ascorbic acid solution, then placed in 1% pheneli, 1% cefosporinic mixture Sterilization was sterilized in the solution for 20 min, then rinsed 5 times with sterile water, dried water with a sterile filter paper to give sterile mulmonterosis.

[0041] Step 2, the preparation of mulberry forming layer tissue

[0042] The mulberridous humeral shot of steps was treated with 10% to 50% sucrose high permeation to obta...

Embodiment 3

[0054] Example 3, using a mulberry tip to cultivate sauna cells

[0055] Step 1, sterile treatment of mulberry tip

[0056] Wash the mulberry tip to clean the surface without residual dirt, dry the surface moisture with filter paper, placed in a sterile operation table for 30 min, then cut the mulberry sprout into a 5 mm small segment, use After the 75% ethanol solution was rinsed for 30 seconds, the ethanol solution was quickly poured, flush 3 times in sterile water, soak 3 min in an ascorbic acid solution of 120 mg / L, then add 1% pheneli, 1% cefosporinine Sterilization was sterilized in a mixed solution for 5 min, then flush 5 times with sterile water, dried water with a sterile filter paper to give a sterile sprouts.

[0057] Step 2, the preparation of mulberry forming layer tissue

[0058] The mulberry exterior of the steps was placed in a sucrose high permeation to obtain a mulberry forming layer tissue.

[0059] Step three, the preparation of sauna cell group

[0060] The ...

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Abstract

The invention discloses a preparation method and application of mulberry stem cells, and the method comprises the following steps: putting mulberry explants into a sucrose high penetrating fluid for treatment to obtain mulberry cambium tissues, planting the mulberry cambium tissues on a solid culture medium to obtain mulberry stem cell clusters, carrying out enzymolysis on the mulberry stem cell clusters, and separating to form single mulberry stem cells, carrying out subculture by utilizing a liquid culture medium, and carrying out amplified culture on the single mulberry stem cells to obtain a mulberry stem cell culture; wherein cellulase and pectinase are adopted in the enzymolysis. The preparation method of the mulberry stem cells has the advantages of obvious differentiation, fast proliferation and short culture period, and the prepared mulberry stem cells have obvious antioxidant, anti-aging and hypoglycemic activity, and can be widely applied to the fields of medicines, cosmetics and nutritional and functional foods.

Description

Technical field [0001] The present invention relates to the field of biotechnology, and more particularly to the preparation method and application of sananogeneous cells. Background technique [0002] Mulberry (Latin Name: Morus Alba L.) is Sahco, mulberry leaves deciduous trees or shrubs. Mulberry leaves (referred to as mulberry leaves) were originally produced in China, South Korea and Japan, so far, 50% of countries have grown around the world. my country is the largest mulberry planting country, mulberry leaves are the main products of mulberry, which covers more than 64% of the above production, so Sang Yes has great generic resources in my country. The mulberry tree is called Shenmu in the "Herd" of the past, and the mulberry leaves are called the fairy leaves in the "Shennong Herb", and the cold, tasteful, with evacuation wind, clear lungs, and clear liver. Mulberry extract contains rich active ingredients such as polysaccharide, flavonoids (rutin, quercetin) and alkaloid...

Claims

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Application Information

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IPC IPC(8): C12N5/04A61K8/99A61K8/9789A61Q19/08A61K36/605A61P3/10A61P39/06
CPCC12N5/04C12N5/0025A61K8/99A61K8/9789A61Q19/08A61K36/605A61P3/10A61P39/06A61K2800/10A61K2800/805
Inventor 武洪淼
Owner 北京添易生物科技有限公司
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