Robinia pseudoacacia tissue culture method

A technology of tissue culture and Sophora japonica, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problem of the complexity of the rooting medium formula of the first generation medium, the influence of the medium on the reproduction coefficient and rooting rate, and the impact on the tissue culture of Sophora japonica Problems such as technology promotion and application, to achieve the effect of easy promotion, good sterilization effect and high growth probability

Inactive Publication Date: 2019-04-02
北京蓉坤农业科技有限公司 +1
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many research reports on the tissue culture of Sophora japonica, the required equipment is more complex, the procedures are cumbersome, the formulas of the first generation medium, the second generation medium and the rooting medium are complex, and some materials are not only expensive but also difficult to purchase, and the cultivation The pros and cons of the base seriously affect the reproduction coefficient and rooting rate, etc.
These have seriously affected the popularization and application of the tissue culture technology of Saccharomyces japonica, and then seriously affected the popularization and application of Sophora japonica

Method used

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Embodiment Construction

[0017] From April to May in spring, when the Saccharomyces japonica plants germinate and take out branches, choose strong growth, no pests and diseases, and strong plants. The thick shoots with axillary buds were taken as explants for tissue culture. In the selected explant branches, use scissors to remove excess leaves, take the 3-4cm long stem segment with 3 axillary buds, put it in a container, rinse it under running water for 30-60min, and get it for inoculation On the stage, first rinse with sterile water 2-3 times, then soak with 75% alcohol for 20-30s, then rinse with sterile water for 3-4 times, then blot the excess water with sterile filter paper. Finally, sterilize with 0.1% mercuric chloride for 3.5-4 minutes, rinse with sterile water 4-5 times for later use. The time and sequence of the treatment method are summarized through a large number of experiments. Compared with the existing technology, the survival rate and treatment effect can be greatly improved. Put t...

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Abstract

The invention discloses a robinia pseudoacacia tissue culture method. The robinia pseudoacacia tissue culture method comprises the following steps: screening an explants; treating the explants; performing the primary culture of the explants; performing the subculture; and performing the rooting culture. The robinia pseudoacacia tissue culture method is popular and easy to understand, simple in operation and easy in popularization; the culture medium is wide in material source and low in cost; the procedures are standard, nodes are clear, and the key technical specifications are detailed; in the process of tissue culture, axillary buds are rapid in germination, the growth probability is high, the greening is fast, the healthy callus can be rapidly formed, and the differentiation is apparent.

Description

technical field [0001] The invention belongs to the technical field of tissue culture, and in particular relates to a method for tissue culture of Sophora japonica. Background technique [0002] Fragrant locust tree (scientific name: Robinia pseudoacacia cv.idaho) is a cultivated variety of Robinia pseudoacacia in the family Fabaceae. It is a deciduous tree with a plant height of up to 12 meters. It has pinnate compound leaves with oval to oval leaves and racemes. Perianth red, with rich aroma, deciduous tree, the whole plant is 10-12 meters high, the trunk is brown to taupe. The leaves are alternate, 7-19 pieces form pinnate compound leaves, the leaves are oval to egg-shaped oblong, 3-6 cm long, larger than black locust leaves. The leaves are beautiful and symmetrical, dark green and shiny, verdant green. Inflorescences densely formed in racemes, pendulous. The perianth is red and has a strong aromatic smell, and 200-500 small red flowers can bloom at the same time. Apo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 刘文虎梁娜刘海涛周晓莹顾鑫
Owner 北京蓉坤农业科技有限公司
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