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Tissue culture and rapid propagation method and device for catalpa bungei

A technology for tissue culture rapid propagation and catalpa tree, applied in the agricultural field, can solve the problems of slow growth, abnormal morphology and physiology of tissue culture plants, and achieve the effects of promoting autotrophic growth, good growth and reducing pollution rate of plants.

Pending Publication Date: 2019-01-04
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In view of this, the embodiment of the present invention provides a method and device for tissue culture and rapid propagation of catalpa, the main purpose of which is to solve the problems of slow growth, morphological and physiological abnormalities of tissue cultured plants

Method used

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  • Tissue culture and rapid propagation method and device for catalpa bungei

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Embodiment 1

[0028] like figure 1 As shown, a catalpa tissue culture rapid propagation device includes: a carbon dioxide generator, its outlet is provided with a valve for controlling gas pressure and flow, and the above-mentioned carbon dioxide generator is used to generate, store and release carbon dioxide gas; A sugar-free medium is provided for inoculating and cultivating Catalpa tree tissue culture seedlings; the inlet of the above-mentioned valve is connected with the outlet of the above-mentioned carbon dioxide generator, and the outlet of the above-mentioned valve is communicated with the gas inlet of the above-mentioned culture box through a pipeline; It is arranged above the above-mentioned cultivation box, and is used for illuminating the above-mentioned cultivation box, and provides a light source for photosynthesis for the above-mentioned catalpa tissue culture seedlings.

[0029] The chemical substances contained in the above-mentioned carbon dioxide generator are citric acid...

Embodiment 2

[0032] The implementation method of embodiment 1 Chinese catalpa tissue culture rapid propagation device:

[0033] 1. Add 200g of citric acid and 200g of sodium bicarbonate into the carbon dioxide generator, then add 300ml of water, and quickly screw on the valve (this reaction is an endothermic reaction, and the temperature drop of the bottle body is a normal reaction), after a period of reaction, the valve is closed The pressure gauge pointer can be used when the scale is 10kg or above, and the pointer scale is about 20kg after the reaction is complete;

[0034] 2. Medium preparation: The medium used in this embodiment 1 is an improved WPM medium, which is composed of inorganic salts and organic matter;

[0035] Inorganic salts include: NH 4 N0 3 400mg / L, Ca(NO 3 ) 2 4H 2 O 556mg / L, K 2 SO 4 990mg / L, CaCl 2 2H 2 O 96mg / L, KH 2 PO 4 170mg / L, Na 2 MoO 4 2H 2 O 0.25mg / L, MgSO 4 ·7H 2 O 370mg / L, MnSO 4 ·H 2 O 22.4mg / L, ZnSO 4 ·7H 2 O 8.6mg / L, CuSO 4 ·5H ...

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Abstract

The invention discloses a tissue culture and rapid propagation method and device for catalpa bungei and relates to the technical field of agriculture. The device comprises a carbon dioxide generator,culture boxes and an LED lamp tube, wherein a valve for controlling flow velocity of carbon dioxide is arranged at an outlet of the carbon dioxide generator, and the carbon dioxide generator is used for producing, storing and releasing carbon dioxide gas; sugar-free media are arranged in the culture boxes, and the culture boxes are used for inoculated culture of catalpa bungei tissue culture seedlings; an inlet of the valve is connected with an outlet of the carbon dioxide generator, and an outlet of the valve is communicated with gas inlets of the culture boxes through pipes; the LED lamp tube is arranged above the culture boxes and used for irradiating the culture boxes and providing light source for photosynthesis of the catalpa bungei tissue culture seedlings. According to the method,the sugar-free media are adopted for culturing the catalpa bungei tissue culture seedlings, carbon dioxide is adopted to replace sugar to serve as a carbon source, and autotrophic growth of the catalpa bungei tissue culture seedlings is realized through photosynthesis. The method has quite low contamination rate, and the sugar-free tissue culture seedlings are higher in survival rate and grow well.

Description

technical field [0001] The invention relates to the field of agricultural technology, in particular to a method and device for tissue culture and rapid propagation of catalpa. Background technique [0002] Catalpa, Bignoniaceae Catalpa, a tall tree with both "material" and appearance, known as "the king of wood", is a precious natural medicinal material in my country. Its stem bark, leaves, seeds and fruits are used as medicine, and its flowers can be used to extract oil. . And because of its strong adaptability, straight wood texture, moderate texture, compact structure, and small drying shrinkage coefficient, it is used as a high-quality material, garden ornamental, and road greening tree species. At present, systematic research has been carried out on the biological characteristics, flowering and fruiting habit, germplasm resource status, improved species selection, propagation technology, and afforestation technology of catalpa at home and abroad, and preliminary results...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王俊臣张森茂王浩陈娟宋珺宇徐久婷王闻竹张一
Owner NORTHWEST A & F UNIV
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