Breeding method for cold-resistant okra

A cold resistance and seed technology, applied in botany equipment and methods, seed immunization, seed and rhizome treatment, etc., can solve the problems of low planting mutation rate, easy loss of genetic traits, uncertain variation direction, etc., to improve immunity Strength and growth rate, improve survival rate, improve the effect of absorption

Inactive Publication Date: 2017-12-15
宁国市绿缘蔬菜种植专业合作社
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention is aimed at the existing problems: planting range, okra has poor cold resistance, and its adaptable growth temperature is high. Although its sowing growth cycle is long, its planting time and range are greatly limited due to the influence of growth temperature; Breeding methods usually adopt mutagenesis and hybridization methods for breeding, but the use of chemical reagents for mutagenesis has strong teratogenic and toxic effects, and the resulting planting mutation rate is low, and causes a large number of seeds to die, and the survival rate is low; the irradiation mutagenesis method The mutation rate is high, but the direction of mutation is uncertain, and many bad genetic traits appear, requiring a lot of screening work; while the hybrid method has limited influence on the genetic traits of okra, and the acquired genetic traits are easily lost

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A breeding method for cold-resistant okra, comprising the following steps:

[0020] (1) Seed storage: detoxify the okra seeds and immerse them in the gibberellin solution for 5.5 minutes to increase the cell viability of the okra seeds, then mix the okra seeds with the culture medium, transfer them to a sealed tank and store them at 9 Store at a constant temperature of ℃ for 9 days. The seeds absorb nutrients, phytohormones and other components in the culture medium, which can promote growth and improve seed immunity. Add colchicine solution to the culture medium, and then store at a constant temperature of 2.5°C for 15 days. Colchicine can induce cold resistance variation of seeds under low temperature conditions to produce stored seeds;

[0021] (2) Mutation breeding: first activate the stored seeds, put them into the atomization chamber and use the active selenium solution for atomization treatment. After the seeds absorb active selenium, the resistance to radiation ...

Embodiment 2

[0032] Present embodiment 2 is compared with embodiment 1, and step changes in the following aspects:

[0033] In the detoxification treatment described in step (1), the detoxification agent is sodium hypochlorite solution with a mass concentration of 60ppm, and the detoxification time is 18min; the described gibberellin solution has a mass concentration of 0.68%; the colchicum solution, its addition amount is 8.4% of the mass of the culture substrate, and its mass concentration is 2.7%.

[0034] Step (1) Seed storage, the method is:

[0035] The okra seeds were mixed with the culture medium, transferred to a sealed jar and stored at a constant temperature of 11°C for 9.5 days, and then stored at a constant temperature of 3.5°C for 16 days.

[0036] The culture substrate described in step (1), its preparation method is:

[0037] Weigh 28 parts of sawdust, 16 parts of lichen, 12 parts of peat, 9.6 parts of kudzu root powder, 6.7 parts of papaya skin, 5.8 parts of nandina, 7.2...

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Abstract

The invention discloses a breeding method of cold-resistant okra, which is characterized in that it includes the following aspects: (1) seed storage, treating the okra seeds with a gibberellin solution, mixing them with a culture medium for storage, and storing them in 2 Heating the colchicine solution at ‑4°C for storage; (2) Mutagenesis breeding, first spraying the seeds with active selenium solution, then irradiating them with ultraviolet light at varying temperatures, and finally irradiating them with gamma rays; (3) Pregermination, immerse the mutagenized seeds in gibberellin solution for constant temperature germination; (4) Breeding, breed and cultivate at a temperature of 3-5°C, and screen cold-resistant seeds; (5) Cross-breeding, mutagenized seeds The okra seeds, the third-generation seeds, and the fourth-generation seeds were crossed three times to obtain the fifth-generation seeds for large-scale planting.

Description

technical field [0001] The invention belongs to the technical field of vegetable planting, and in particular relates to a breeding method of cold-resistant okra. Background technique [0002] Okra is an annual herb, also known as okra, coffee ambrette, and okra. It is divided into two types: green and red. It is widely planted in South Asia; its habit is warm climate, not cold-resistant, and it is deep, fertile and sunny land. Okra is rich in protein, fat, carbohydrates, vitamins, minerals and other nutrients. Long-term consumption can improve human immunity, protect gastric mucosa, and have certain curative effects on sore throat, urination and diabetes. Good food taste and health value. But the cold resistance of okra is relatively poor, and its adaptable growth temperature is high, although its sowing growth cycle is long, its planting time and scope are all subject to relatively large restrictions due to the influence of growth temperature; Related, the method of muta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06A01H1/02A01C1/00A01C1/08
CPCA01H1/06A01C1/00A01C1/08A01H1/02
Inventor 郭芳
Owner 宁国市绿缘蔬菜种植专业合作社
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