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Preparation method of biostimulant and application thereof

A biostimulant and crop technology, applied in the direction of fertilizers, applications, and fertilizer mixtures made from biological waste, can solve the problems of rare invention patents, achieve low application costs, simple cultivation and production processes, and improve plant resistance Effect

Inactive Publication Date: 2019-03-22
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, some domestic invention patents have been obtained in the technology of alleviating the symptoms of plant deficiency symptoms, but there are not many invention patents on the use of microbial fermentation technology to prepare related plant biostimulants containing active substances

Method used

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  • Preparation method of biostimulant and application thereof
  • Preparation method of biostimulant and application thereof
  • Preparation method of biostimulant and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A biostimulant obtained by the following steps: taking 3% to 5% EM stock solution, 2% to 5% brown sugar, 5% to 10% 20% calcium magnesium phosphate fertilizer suspension, 0.5% to 1 % of protein powder and 79% to 89.5% of deionized water are mixed evenly, and placed in a rotary shaker at a speed of 120r / min to 180r / min under airtight conditions at room temperature for anaerobic fermentation at 35 to 37°C for 5-7 days. Stand still, take the supernatant and further filter to separate the solid from the liquid, concentrate the filtrate for 35 to 60 minutes at a temperature of 50 to 75°C and a pressure of -1.5 to -0.5 MPa, and spray dry the concentrate to obtain biostimulant.

[0028] The 20% calcium magnesium phosphate fertilizer suspension is to weigh 200 grams of industrial calcium magnesium phosphate fertilizer in a 500 milliliter beaker, add deionized water and heat to dissolve, and finally settle to a 1000 milliliter volumetric flask.

[0029] The brown sugar and prote...

Embodiment 2

[0031]Mix the stock solution of EM bacteria, molasses, 20% calcium magnesium phosphate fertilizer suspension, protein powder, and deionized water at 5%: 5%: 6%: 1%: 83%, and put them in at 35°C under airtight conditions. Anaerobic fermentation on a rotary shaker at 150r / min for 7 days, the solid and liquid were separated by standing and filtering, and the supernatant was taken at a temperature of 55°C and a pressure of -1.5MPa, and the filtrate was concentrated for 60 minutes. The liquid is spray-dried to obtain a biostimulant containing active substances and complex microorganisms. In order to verify the effect of biostimulants, and to evaluate their effects on tomato navel rot control and yield increase, a field experiment was carried out in the greenhouse of Nanjing Institute of Vegetable Science. The soil texture is heavy loam, and the tested tomato variety is "Yingshi Dahong". The seedlings were raised in the greenhouse using plug-in substrates, and the plants were plante...

Embodiment 3

[0043] Mix the stock solution of EM bacteria, molasses, 20% calcium magnesium phosphate fertilizer suspension, protein powder, and deionized water at 4%: 4%: 8%: 0.8%: 83.2%, and put them in at 37°C under airtight conditions. Anaerobic fermentation on a rotary shaker at 180r / min for 5 days, solid-liquid separation by standing and filtering, concentration of the filtrate for 60 minutes at a temperature of 65°C and a pressure of -1.0MPa, and spray drying of the concentrate. That is, a biostimulant containing active substances and complex microorganisms is obtained. The green pepper plot experiment was carried out in the greenhouse of the Nanjing Institute of Vegetable Science. The soil texture is heavy loam, and the agronomic measures such as fertilization and irrigation between the acidification and salt preparation treatments are consistent with the field management. The experiment set up 3 treatments, each The treatments were repeated 3 times and arranged in random blocks. T...

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Abstract

The invention discloses a preparation method of biostimulant and application thereof. The preparation method comprises the following steps of selecting 3% to 5% of EM (effective microorganism) raw liquid, 2% to 5% of carbon source, 5% to 10% of 20% calcium-magnesium-phosphor fertilizer suspension, 0.5% to 1% of nitrogen source, and 79% to 89.5% of chloride-free water, and uniformly mixing; puttinginto a rocking bed, fermenting for 5 to 7d at the temperature of 35 to 37 DEG C in an anaerobic way, standing, and filtering to separate solid and liquid; concentrating filtrate for 35 to 60 min at the pressure of -1.5 to -0.5 MPa, and spray-drying the concentrated liquid, so as to obtain the biostimulant. The biostimulant has the advantages that the calcium-magnesium-phosphor fertilizer and theprotein powder which are difficult to dissolve into water are converted into chelating calcium and magnesium, small-molecular amino acid and organic phosphor which are water-soluble, so that the absorbing and utilization efficiency of calcium, magnesium and mineral nutrients is improved, and the physiological barriers caused by lack of calcium, magnesium and the like in plants can be effectively solved, such as tomato blossom-end rot and chlorosis of green pepper; by irrigating to roots, the physicochemical property of the soil is improved, and the soil water retention effect and nutritional element activation effect are better.

Description

technical field [0001] The invention belongs to the technical field of multifunctional biological preparations, in particular to a biostimulant that can effectively reduce the effects of physiological disorders caused by plant calcium and magnesium deficiency. technical background [0002] Biostimulant (Biostimulant) is a substance that contains certain active ingredients and microorganisms that, when applied to plant leaves or rhizosphere soil, stimulate natural processes in plants, including beneficial Nutrient absorption, improvement of nutritional efficacy, enhancement of abiotic stress resistance and improvement of crop quality, it is different from fertilizer, its function has nothing to do with nutrients, but with active substances. [0003] Biostimulants are a new type of product that has emerged in the global agricultural materials market in recent years. According to statistics, the market value of biostimulants in 2015 was about 1.46 billion US dollars. It is pred...

Claims

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Application Information

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IPC IPC(8): C05G3/00C05G3/04C05G3/80
CPCC05B13/02C05G3/80C05G5/20C05C11/00C05F5/00C05F11/08
Inventor 邵雅璐钟华
Owner WUHAN UNIV
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