68results about How to "The cultivation process is simple" patented technology

A process for puffing the culture medium of edible fungus, which is prepared from the twigs or the straw or stalk of agricultural crops includes such steps as pulverizing, proportionally mixing it with organic and inorganic auxiliaries, puffing and baking.

The invention provides a culture medium for cultivating coprinus comatus, which is composed of chestnut shells, crop straws or corncobs, dry livestock manure, calcium superphosphate, quick lime, enzyme bacterium and water. The method utilizing the culture medium to cultivate the coprinus comatus comprises the following steps: first, uniformly stirring all raw materials to produce a base material; second, stacking the base material for fermentation; third, bagging the fermented base material for inoculation so as to produce bacteria sticks; fourth, placing the bacteria sticks in a bacteria cultivating room; fifth, debagging the bacteria sticks so as to allow the bacteria sticks to be stood in a bed and is covered with soil; sixth, carrying out water spray management; and seventh, picking the coprinus comatus. According to the invention, the chestnut shells are basic material of the culture medium which is green and pollution-free, the cultivating process is simple, and the production cost is low; and the coprinus comatus is high in yield, good in quality, and high in biotransformation efficiency.

The invention discloses an endophytic fungus Phomopsis sp. strain ZJWCF252, which was collected with a name of Phomopsiswenchengensis and a collection number of CGMCCNo.4301 in China General Microbiological Culture Collection Center of Institute of Microbiology of Chinese Academy of Sciences, which is located in building No.3, Yard No.1, Beichen Road, Chaoyang District, Beijing, on November 1st, 2010. The invention also discloses the use of the endophytic fungus Phomopsis sp. in the preparation of 2,3-dihydro-2-hydroxy-2,4-dimethyl-5-trans-propenylfuran-3-one which has strong inhibition effects on various plant pathogenic fungi.

The invention belongs to the technical field of multifunctional biological preparations, and provides a method for preparing a compound microbial calcium agent. The method comprises the following preparation step of: adding a 10 percent calcium sulfate suspension in a process for preparing an active liquid of compound microorganisms, and comprises the following specific steps of: compounding 3-5 percent of an EM stock solution, 2-5 percent of honey, 5-10 percent of a calcium sulfate suspension and 80-92 percent of deionized water according to volume proportions, and mixing uniformly; putting into a rotary shaking table of which the speed is 120-180 revolutions per minute under a room temperature enclosed condition for performing anaerobic fermentation for 3-5 days; and standing and filtering for separating solid from liquid to obtain a liquid preparation of compound microbial calcium. The method has the advantages that: the fermented calcium is in a chelated (complexed) state or a cation-anion balance state, is dissolved into water, and is easy to be absorbed by plants through foliar application, and physiological disorder (such as tomato fruit blossom-end rot caused by the lack of calcium) caused by the lack of calcium in plants can be eliminated effectively.

The invention discloses a culture method for pleurotus nebrodensis. The culture method comprises the following steps: culture material preparation, bagging, sterilization, inoculation, spore germination, post-ripening management, fruiting management, and harvesting and packaging. The culture method provided by the invention has the advantages of simple process, low cost, availability of spare room for production, fast spore germination, high biological transformation efficiency, tidy fruiting, high economic benefits, etc., and solves the problems like unstable yield and quality in the production of artificial culture.

The invention relates to a method for effectively removing odors in refuse landfills or refuse transfer stations, belonging to the technical field of multifunctional biological agents. Compound biological deodorant concentrated solution is obtained by mixing 4-5% of EM (effective microorganisms) stock solution, 3-5% of molasses, 3-5% of plant essence-ethanol mixed solution and 85-90% of water uniformly and putting the mixture into a rotary table at a speed of 120-180r/min to undergo anaerobic fermentation for 3-5 days under the aright condition at room temperature. The compound biological deodorant solution has the advantages of simple preparation process and low cost, does not cause secondary pollution and has a simple and convenient use method and broad application prospect.

The present invention relates to a process for producing flavone through liquid-solid double-phase culture of medicinal mushroom phellinus igniarius, and belongs to the technical field of biological fermentation engineering. According to the present invention, mainly during a phellinus igniarius liquid fermentation process, a macroporous absorption resin is added to a culture liquid to establish a liquid-solid double-phase culture system so as to improve a flavone yield; with the double-phase culture process, the flavone yield can be up to 2174.35 mug/ml and is increased by 35% compared with the control; and the process has characteristics of simple operation and strong generalization, and is especially suitable for large-scale higher fungi fermentation and active substance production.

The present invention belongs to the field of biotechnology, and discloses glutamine synthetase-deficient cell line HEK293-GS-/-which is based on HEK293 cells, can be stably passaged, is adapted to suspension culture, can be applied to the expression of recombinant proteins, and is constructed by using of a CRISPR/Cas9 system. The glutamine synthetase-deficient cell line HEK293-GS-/-can screen engineered cell strains expressing various recombinant proteins through a GS/MSX screening system. The glutamine synthetase-deficient cell line HEK293-GS-/-can stably express the target proteins after multiple passages; constructed cells can be adapted to most of commercial serum-free media; the screened cells themselves express high levels of glutamine synthetase, and the upstream culture process isgreatly simplified. The glutamine synthetase-deficient cell line HEK293-GS-/-can be applied to molecular biology, cell biology and other research fields, and is also suitable for constructing engineering cells used in the field of biopharmaceuticals.

The invention discloses a method for interplanting Chinese mahonia in anise forest land, and belongs to the technical field of traditional Chinese medicinal material cultivation. The method particularly includes the seven steps that the anise forest land is selected, anise trees are clipped and are subject to intermediate cutting, soil preparation is carried out, base fertilizer is applied, transplanting is carried out, intertillage weeding is carried out, and fertilizer is applied. The defects that when the Chinese mahonia is manually cultivated, the land resource is occupied, the yield is low, and the cultivating process is complex are overcome; the method has the beneficial effects that the land resource is saved, manual shed building for shading is not needed, the cultivating process is simple, the cultivating cost is reduced by 10%-15%, the yield is improved by 5%-6%, the current condition that the wild Chinese mahonia resource is exhausted, and production and income increase of anise is remarkable.

The invention provides a Vero cell line for stably secreting and expressing bovine trypsinogen (S.pro-try) and application thereof to influenza virus culture and influenza virus vaccine production. The Vero cell line is named as VTY55, has the preservation number of CGMCC No.12680, and belongs to the technical field of biology. The invention also provides a method for building the Vero-S.pro-try cell line. The Vero-S.pro-try monoclonal cell line VTY55 provided by the invention has the advantages that on one hand, the cell foundation is laid for the large-scale influenza vaccine production in future, and the crucial problems of vaccine productivity expansion and quality improvement can be solved; on the other hand, the cell line can also be used as a study tool in other aspects such as laboratory effective ingredient separation culture, influenza virus monitoring, anti-influenza-virus medicine screening, neutralizing antibody determination and the like; wide application prospects are realized.

The present invention relates to an open-type edible fungus cultivation method. Said invention adopts fermentation method to cultivate domestic fungus, its cultural material only has need of once stacking fermentation, and its cultispecies can adopt dominant strain cultured after the nutrient additive granules are added in the strain cultural material. Said method is applicable to production of various edible fungi.

The invention discloses a strain of photosynthetic bacterium Rhodobacter capsulatus, a bactericide agent as well as a preparation method and an application of the bactericide agent. The strain of the photosynthetic bacterium Rhodobacter capsulatus is photosynthetic bacterium Rhodobacter capsulatus A12, and Rhodobacter capsulatus A12 is collected in CCTCC (China Center for Type Culture Collection) with the collection number of CCTCC No:M 2018604. The culture process for the photosynthetic bacterium Rhodobacter capsulatus A12 is simple, the culture time is short, the cost is low, and a biocontrol bactericide and biocontrol fermentation liquor for control of rice bacterial leaf blight are prepared from the strain of the photosynthetic bacterium Rhodobacter capsulatus with a simple and fast low-cost operating method.

The invention discloses a method of culturing eurotium cristatum on a coffee bean solid medium. According to the method, a solid medium is taken as the culture medium; eurotium cristatum powder is cultured in a coffee bean solid culture medium; the culture technology is simple, the culture conditions can be more easily controlled, the manufacturing effect is more stable and better, and the cultured eurotium cristatum powder is used to prepare eurotium cristatum coffee.

The present invention discloses a culture method of cordyceps militaris. The method comprises the following steps: step 1, activating a culture of cordyceps militaris; step 2 carrying out inoculated culture; step 3 carrying out management of producing mushrooms; and step 4, harvesting. The culture method disclosed by the present invention is simple in process and low in cost, and a free room can be used to produce, so that the problems that the output and the quality in artificial culture and production are instable and the like are solved.

The invention discloses a photosynthetic bacterium rhodobacter sphaeroides strain, a bacterial drug agent, and a preparation method and an application of the bacterial drug agent. The photosynthetic bacterium rhodobacter sphaeroides strain is rhodobacter sphaeroides A13, is preserved in China Center for Type Culture Collection and has the preservation number of CCTCC No:M 2018605. The photosynthetic bacterium rhodobacter sphaeroides strain, the rhodobacter sphaeroides A13, has the advantages of simple cultivation process, short cultivation time and low cost; and a biocontrol agent and a biocontrol fermentation liquid for prevention and control of paddy rice bacterial leaf streak can be prepared from the photosynthetic bacterium rhodobacter sphaeroides strain by the simple, fast and low-cost operation method.