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68results about How to "The cultivation process is simple" patented technology

Bulking processing art for producing edible mushroom culturing material by using tree-branch and stalks as raw materials

A process for puffing the culture medium of edible fungus, which is prepared from the twigs or the straw or stalk of agricultural crops includes such steps as pulverizing, proportionally mixing it with organic and inorganic auxiliaries, puffing and baking.
Owner:程宽

Pholiota nameko culture method

InactiveCN102613006ATake full advantage of the mushrooming effectRaw materials are easy to getHorticultureFertilizer mixturesPholiotaMycelium
The invention relates to a pholiota nameko culture method, which comprises the steps of: culture material proportioning and preparation, sterilization, inoculation and bagging, mycelium culture, summer-crossing management and primordium inducement fruiting. The pholiota nameko culture materials comprise the following recipe and mixture ratios: 30 to 50 parts of corncobs, 30 to 50 parts of soft mixed hardwood sawdust, 10 to 20 parts of bran, 2 to 4 parts of corn starch, 0.5 to 1.5 parts of gypsum and 0.5 to 1.5 parts of lime. The culture raw materials adopt the corncobs and the soft mixed hardwood sawdust as major materials, a good basis is laid for the smooth implementation of the Natural Forest Protection Projects and the pholiota nameko planting in flat areas, planting trays are omitted, the culture cost can be saved by 15 percent, the culture process is simple and is easy to implement, the work efficiency is improved, the edge fruiting effect of the pholiota nameko bag type culture is improved by 30 percent through being compared with that of the disc type culture, the edge fruiting effect of the edible fungi is sufficiently utilized, the yield can be improved by more than 10 percent, and the high-quality fruiting rate is improved by more than 40 percent.
Owner:沈阳蕈丰食用菌科技有限公司

Culture medium and cultural method for cultivating coprinus comatus

The invention provides a culture medium for cultivating coprinus comatus, which is composed of chestnut shells, crop straws or corncobs, dry livestock manure, calcium superphosphate, quick lime, enzyme bacterium and water. The method utilizing the culture medium to cultivate the coprinus comatus comprises the following steps: first, uniformly stirring all raw materials to produce a base material; second, stacking the base material for fermentation; third, bagging the fermented base material for inoculation so as to produce bacteria sticks; fourth, placing the bacteria sticks in a bacteria cultivating room; fifth, debagging the bacteria sticks so as to allow the bacteria sticks to be stood in a bed and is covered with soil; sixth, carrying out water spray management; and seventh, picking the coprinus comatus. According to the invention, the chestnut shells are basic material of the culture medium which is green and pollution-free, the cultivating process is simple, and the production cost is low; and the coprinus comatus is high in yield, good in quality, and high in biotransformation efficiency.
Owner:柞水县海林菌业有限责任公司 +1

Hericium erinaceus cultivation method

The invention discloses a hericium erinaceus cultivation method comprising the steps of culture material preparation, fungi bag preparation, sterilization, inoculation, greenhouse construction, mycelium culturing, fruiting management, harvesting, and post management. The cultivation process provided by the invention is simple, and the cost is low. The cultivated hericium erinaceus has the advantages of fast mycelium germination, robust growth, uniform and neat primordium, ordered fruiting body growth condition, robust fruiting body growth, and high biological efficiency.
Owner:吴中区胥口精益生物医药研究所

Endophytic fungus Phomopsis sp. and use thereof

The invention discloses an endophytic fungus Phomopsis sp. strain ZJWCF252, which was collected with a name of Phomopsiswenchengensis and a collection number of CGMCCNo.4301 in China General Microbiological Culture Collection Center of Institute of Microbiology of Chinese Academy of Sciences, which is located in building No.3, Yard No.1, Beichen Road, Chaoyang District, Beijing, on November 1st, 2010. The invention also discloses the use of the endophytic fungus Phomopsis sp. in the preparation of 2,3-dihydro-2-hydroxy-2,4-dimethyl-5-trans-propenylfuran-3-one which has strong inhibition effects on various plant pathogenic fungi.
Owner:ZHEJIANG DAYANG BIOTECH GROUP

Japanese Grifola frondosa cultivating method

A Japanese Grifola frondosa cultivating method specifically comprises steps as follows: selecting wood chips of broad-leaf species as a main cultivation material, and selecting wheat bran and Indian meal as nutrition sources; and then performing material mixing, bagging sterilization, inoculation, cultivation management, fruiting management and harvesting according to routine methods. During the cultivation management process, the temperature in a cultivate chamber is maintained to be 22-23 DEG C, the humidity is 60%-70%, and when white hypha lumps are formed on the surface of a fungus bag, gray primordium are formed and transparent water droplets are formed on the surface, 50 Lux lighting stimulation is required; and in a fruiting management process, when all the primordiums change into black and the water droplets are reduced, a ventilation silicone window at the upper part of the bag is required to be cut open by a cutter blade promptly, meanwhile, the temperature of the fruiting chamber is maintained to be 16-18 DEG C, the humidity is above 95%, and the lighting condition is maintained to be 500 Lux. With the implementation of the Japanese Grifola frondosa cultivating method, the condition that the precedent that industrial cultivation of the Grifola frondosa inland is not started yet at present is broken through, further, the production efficiency is quite high, and the market benefits are quite good.
Owner:覃秀花

Preparation method of compound microbial calcium agent

The invention belongs to the technical field of multifunctional biological preparations, and provides a method for preparing a compound microbial calcium agent. The method comprises the following preparation step of: adding a 10 percent calcium sulfate suspension in a process for preparing an active liquid of compound microorganisms, and comprises the following specific steps of: compounding 3-5 percent of an EM stock solution, 2-5 percent of honey, 5-10 percent of a calcium sulfate suspension and 80-92 percent of deionized water according to volume proportions, and mixing uniformly; putting into a rotary shaking table of which the speed is 120-180 revolutions per minute under a room temperature enclosed condition for performing anaerobic fermentation for 3-5 days; and standing and filtering for separating solid from liquid to obtain a liquid preparation of compound microbial calcium. The method has the advantages that: the fermented calcium is in a chelated (complexed) state or a cation-anion balance state, is dissolved into water, and is easy to be absorbed by plants through foliar application, and physiological disorder (such as tomato fruit blossom-end rot caused by the lack of calcium) caused by the lack of calcium in plants can be eliminated effectively.
Owner:益睦生物科技(南京)有限公司

Culture method for pleurotus nebrodensis

The invention discloses a culture method for pleurotus nebrodensis. The culture method comprises the following steps: culture material preparation, bagging, sterilization, inoculation, spore germination, post-ripening management, fruiting management, and harvesting and packaging. The culture method provided by the invention has the advantages of simple process, low cost, availability of spare room for production, fast spore germination, high biological transformation efficiency, tidy fruiting, high economic benefits, etc., and solves the problems like unstable yield and quality in the production of artificial culture.
Owner:吴中区胥口精益生物医药研究所

Nutrient additive grain of edible fungus

A granular nutritive additive for culturing edible fungus is composed of core containing crude protein and the water-insoluble coated layer which can be decomposed into carbonhydrate by the mycelia of edible fungus. It can be added to the culture medium of edible fungus to greatly increase the yield of edible fungus.
Owner:郑祥品

Method for preparing chelating magnesium

InactiveCN102491797AEliminate symptoms of magnesium deficiencyPrevent color abnormalitiesOrganic fertilisersBiotechnologyChlorophyllin
The invention belongs to the technical field of multifunctional foliage fertilizer and provides a method for preparing chelating magnesium. The preparation steps are as follows: preparing 20% of magnesium carbonate suspension first by volume percent, and adding the 20% of magnesium carbonate suspension during the preparation process of compound microbial activated solution; and the specific method comprises the steps of combining the following materials by volume ratio: 3 % to 5% of EM stock solution, 2% to 5% of honey, 25% to 30% of the magnesium carbonate suspension and 60% to 70% of deionized water, blending the mixture uniformly, and putting the mixture in a rotary shaking table with the speed of 120 r / min to 180 r / min and fermenting for 6 to 7 days under ambient temperature. Therefore, chelating magnesium can be obtained. The method has the advantages that the magnesium fermented with compound microbe is in a chelating (complexation) state or an anion cation balance state, is easily insoluble in water, and is easy to be absorbed by plants; and when the chelating magnesium is applied on leaves, the chlorophyl content of vegetables can be improved, and the problem of physiological barrier of plants caused by lack of magnesium can be solved effectively.
Owner:HOHAI UNIV

Method for transforming 1,3-propylene glycol into 3-hydroxypropionic acid by applying resting cells

The invention discloses a method for transforming 1,3-propylene glycol into 3-hydroxypropionic acid by applying resting cells, and belongs to the field of biological engineering. Through the method, firstly a rapid screening method is utilized to screen a microbial strain which has high transformation performance and high production capacity and is identified as acetobacter sp. JDB-71, and the resting cells of the strain are utilized for high-efficiency catalysis of 1,3-propylene glycol into 3-hydroxypropionic acid in an aqueous phase system. The obtained acetobacter sp. culture process is simple, the transformation efficiency is high, and the product yield is large; transformation solution components are simple, so as to be benefitial for separation and purification of the product; and the thalli concentration can be artificially adjusted to shorten the production time during the transformation process, and thus a lot of energy and production costs are saved.
Owner:JIANGNAN UNIV

Compound biological deodorant solution used in refuse landfills and refuse transfer stations

The invention relates to a method for effectively removing odors in refuse landfills or refuse transfer stations, belonging to the technical field of multifunctional biological agents. Compound biological deodorant concentrated solution is obtained by mixing 4-5% of EM (effective microorganisms) stock solution, 3-5% of molasses, 3-5% of plant essence-ethanol mixed solution and 85-90% of water uniformly and putting the mixture into a rotary table at a speed of 120-180r / min to undergo anaerobic fermentation for 3-5 days under the aright condition at room temperature. The compound biological deodorant solution has the advantages of simple preparation process and low cost, does not cause secondary pollution and has a simple and convenient use method and broad application prospect.
Owner:HOHAI UNIV

Process for producing flavone through liquid-solid double-phase phellinus igniarius culture

The present invention relates to a process for producing flavone through liquid-solid double-phase culture of medicinal mushroom phellinus igniarius, and belongs to the technical field of biological fermentation engineering. According to the present invention, mainly during a phellinus igniarius liquid fermentation process, a macroporous absorption resin is added to a culture liquid to establish a liquid-solid double-phase culture system so as to improve a flavone yield; with the double-phase culture process, the flavone yield can be up to 2174.35 mug / ml and is increased by 35% compared with the control; and the process has characteristics of simple operation and strong generalization, and is especially suitable for large-scale higher fungi fermentation and active substance production.
Owner:CHINA UNIV OF PETROLEUM (EAST CHINA)

Use of 3, 4 dihydro-8-hydroxy-1-hydro-2-benzopyran 1 derivative for inhibiting plant pathogenic fungus

The invention relates to the application of 3, 4dihydro 8 hydroxyl 1 benzopyrane 1 ramification extracted from Beijing mutation metabolite of Xenorhabdus nematophila pathogenic bacillus to prohibiting plant pathogenic fungi, dissolving the benzopyrane ramification freeze dried pure products in axenic water, mixing into a mother solution in 1000 mug / ml, then separately using PDA culture medium to dilute the mother solution into solutions in 0.20 mug / ml-20 mug / ml. The benzopyrane ramification extracting technique is simple and the produced bacteria are easy to culture in batches; the benzopyrane ramification has stronger bacteriostatic activity to various plant pathogenic fungi, especially to plant Phytophthora palmi; and this compound is firstly used to prohibit plant pathogenic fungi, and its bacteriostatic activity is strong, and it is convenient and safe to use.
Owner:INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI

Method for screening glutamine synthetase-deficient HEK293 cell line

The present invention belongs to the field of biotechnology, and discloses glutamine synthetase-deficient cell line HEK293-GS- / -which is based on HEK293 cells, can be stably passaged, is adapted to suspension culture, can be applied to the expression of recombinant proteins, and is constructed by using of a CRISPR / Cas9 system. The glutamine synthetase-deficient cell line HEK293-GS- / -can screen engineered cell strains expressing various recombinant proteins through a GS / MSX screening system. The glutamine synthetase-deficient cell line HEK293-GS- / -can stably express the target proteins after multiple passages; constructed cells can be adapted to most of commercial serum-free media; the screened cells themselves express high levels of glutamine synthetase, and the upstream culture process isgreatly simplified. The glutamine synthetase-deficient cell line HEK293-GS- / -can be applied to molecular biology, cell biology and other research fields, and is also suitable for constructing engineering cells used in the field of biopharmaceuticals.
Owner:EUREKA BIOTECH LTD

Method for interplanting Chinese mahonia in anise forest land

The invention discloses a method for interplanting Chinese mahonia in anise forest land, and belongs to the technical field of traditional Chinese medicinal material cultivation. The method particularly includes the seven steps that the anise forest land is selected, anise trees are clipped and are subject to intermediate cutting, soil preparation is carried out, base fertilizer is applied, transplanting is carried out, intertillage weeding is carried out, and fertilizer is applied. The defects that when the Chinese mahonia is manually cultivated, the land resource is occupied, the yield is low, and the cultivating process is complex are overcome; the method has the beneficial effects that the land resource is saved, manual shed building for shading is not needed, the cultivating process is simple, the cultivating cost is reduced by 10%-15%, the yield is improved by 5%-6%, the current condition that the wild Chinese mahonia resource is exhausted, and production and income increase of anise is remarkable.
Owner:莫玉明

Vero cell line for stably expressing bovine trypsinogen (S.pro-try) and purpose thereof

The invention provides a Vero cell line for stably secreting and expressing bovine trypsinogen (S.pro-try) and application thereof to influenza virus culture and influenza virus vaccine production. The Vero cell line is named as VTY55, has the preservation number of CGMCC No.12680, and belongs to the technical field of biology. The invention also provides a method for building the Vero-S.pro-try cell line. The Vero-S.pro-try monoclonal cell line VTY55 provided by the invention has the advantages that on one hand, the cell foundation is laid for the large-scale influenza vaccine production in future, and the crucial problems of vaccine productivity expansion and quality improvement can be solved; on the other hand, the cell line can also be used as a study tool in other aspects such as laboratory effective ingredient separation culture, influenza virus monitoring, anti-influenza-virus medicine screening, neutralizing antibody determination and the like; wide application prospects are realized.
Owner:NAT INST FOR FOOD & DRUG CONTROL

Open domestic fungus cultivating method

The present invention relates to an open-type edible fungus cultivation method. Said invention adopts fermentation method to cultivate domestic fungus, its cultural material only has need of once stacking fermentation, and its cultispecies can adopt dominant strain cultured after the nutrient additive granules are added in the strain cultural material. Said method is applicable to production of various edible fungi.
Owner:郑祥品

Base bait formula for mytilus crassitesta planktonic larva

ActiveCN103598477ASolve the problem of planktonic feedImprove survival rateAnimal feeding stuffIsochrysis galbanaNannochloris oculata
The invention discloses a base bait formula for mytilus crassitesta planktonic larva. The base bait formula comprises the following components according to the parts of a water body containing monodus subterraneus of same cell density: 65-72 parts of water body containing isochrysis galbana parke, 8-16 parts of water body containing nannochloris oculata or chlorella or a mixture of the nannochloris oculata and the chlorella, and 16-24 parts of water body containing diatom. The base bait formula has the advantages of complete nutrition, good luring property, low bait coefficient, low cost and convenience in culture.
Owner:ZHEJIANG OCEAN UNIV

Strain of photosynthetic bacterium Rhodobacter capsulatus, bactericide agent as well as preparation method and application of bactericide agent

The invention discloses a strain of photosynthetic bacterium Rhodobacter capsulatus, a bactericide agent as well as a preparation method and an application of the bactericide agent. The strain of the photosynthetic bacterium Rhodobacter capsulatus is photosynthetic bacterium Rhodobacter capsulatus A12, and Rhodobacter capsulatus A12 is collected in CCTCC (China Center for Type Culture Collection) with the collection number of CCTCC No:M 2018604. The culture process for the photosynthetic bacterium Rhodobacter capsulatus A12 is simple, the culture time is short, the cost is low, and a biocontrol bactericide and biocontrol fermentation liquor for control of rice bacterial leaf blight are prepared from the strain of the photosynthetic bacterium Rhodobacter capsulatus with a simple and fast low-cost operating method.
Owner:HUNAN PLANT PROTECTION INST

Method of culturing eurotium cristatum on coffee bean solid medium

The invention discloses a method of culturing eurotium cristatum on a coffee bean solid medium. According to the method, a solid medium is taken as the culture medium; eurotium cristatum powder is cultured in a coffee bean solid culture medium; the culture technology is simple, the culture conditions can be more easily controlled, the manufacturing effect is more stable and better, and the cultured eurotium cristatum powder is used to prepare eurotium cristatum coffee.
Owner:刘翔

Culture method of cordyceps militaris

The present invention discloses a culture method of cordyceps militaris. The method comprises the following steps: step 1, activating a culture of cordyceps militaris; step 2 carrying out inoculated culture; step 3 carrying out management of producing mushrooms; and step 4, harvesting. The culture method disclosed by the present invention is simple in process and low in cost, and a free room can be used to produce, so that the problems that the output and the quality in artificial culture and production are instable and the like are solved.
Owner:吴中区胥口精益生物医药研究所

Photosynthetic bacterium rhodobacter sphaeroides strain, bacterial drug agent, and preparation method and application of bacterial drug agent

The invention discloses a photosynthetic bacterium rhodobacter sphaeroides strain, a bacterial drug agent, and a preparation method and an application of the bacterial drug agent. The photosynthetic bacterium rhodobacter sphaeroides strain is rhodobacter sphaeroides A13, is preserved in China Center for Type Culture Collection and has the preservation number of CCTCC No:M 2018605. The photosynthetic bacterium rhodobacter sphaeroides strain, the rhodobacter sphaeroides A13, has the advantages of simple cultivation process, short cultivation time and low cost; and a biocontrol agent and a biocontrol fermentation liquid for prevention and control of paddy rice bacterial leaf streak can be prepared from the photosynthetic bacterium rhodobacter sphaeroides strain by the simple, fast and low-cost operation method.
Owner:HUNAN PLANT PROTECTION INST

Stropharia rugoso-annulata culture medium and preparation method and application thereof

The invention discloses a stropharia rugoso-annulata culture medium and a preparation method and application thereof.According to the stropharia rugoso-annulata culture medium, perishable organic waste is adopted as raw materials and comprises garden waste and pre-meal waste, and the garden waste comprises but not limited to branches and fallen leaves; the pre-meal garbage comprises but is not limited to fruits, vegetables, food leftovers and melon and fruit peels discarded in family daily life. According to the method, the perishable organic waste is used for manufacturing the culture medium to replace a traditional stropharia rugoso-annulata culture medium raw material, the idea of resource cyclic utilization is exerted and practiced to the maximum extent, the resource utilization level of the perishable organic waste is greatly improved, and the new mode that non-woven bags are used for indoor culture is achieved; therefore, a traditional stropharia rugoso-annulata cultivation mode is broken, the cultivation technology is simplified, and the cultivation process is optimized and simplified.
Owner:蔚复来(浙江)科技股份有限公司

Method for growing dendrobium seedlings

The invention provides a method for growing dendrobium seedlings, which includes in vitro inoculation of seeds, formation of circular corms through cultivation, and formation of differentiation seedlings by the cultivation of the circular corms, and is characterized by steps as follows: inoculating the differentiation seedlings on a 1 / 2 MS culture medium in a seedling raising bottle; covering with a lid; sealing the part of the lid which is connected with the bottle body with a fresh-keeping film; then placing the bottle in an ordinary greenhouse with a shading rate of 60-80% and after growing in a natural lighting environment with a temperature of 5-34 DEG and illumination intensity of 3500-9000Lx for 2 months, taking the seedlings out of the bottle and permanent-planting in a field. Theinvention can save not only the electric energy consumed in the commodity seedling culturing phase, but also the cost incurred by sterilization, sterilization medicine and manpower, and is convenientto be operated, time-saving and labor-saving. Particularly, after the culturing in the natural lighting environment, the antibacterial and disease-resistant capability of the commodity seedlings is improved, and the planting survival rate of dendrobium seedlings is greatly increased and attains over 90%.
Owner:红河群鑫石斛种植有限公司
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