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Process for producing flavone through liquid-solid double-phase phellinus igniarius culture

A technology of phellinus and flavonoids, applied in the field of biological fermentation engineering, to achieve the effect of increasing yield and simple cultivation process

Inactive Publication Date: 2014-03-12
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method of utilizing liquid-solid two-phase culture to improve Phellinus flavone production has not been reported in the literature, so it has a good development prospect

Method used

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  • Process for producing flavone through liquid-solid double-phase phellinus igniarius culture
  • Process for producing flavone through liquid-solid double-phase phellinus igniarius culture
  • Process for producing flavone through liquid-solid double-phase phellinus igniarius culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Sterilize the DM130 macroporous adsorption resin, the sterilization condition is 121°C, 20min;

[0019] (2) Add the Phellinus strain into the liquid fermentation medium, carry out shaker culture (28°C, 150rpm), and add 50g / 100ml of DM130 macroporous adsorption when the culture time is 3, 4, 5 or 6 days respectively Resin, then continue to cultivate, and set up blank control;

[0020] (3) Filtrate to obtain the resin after 7 days of fermentation, desorb with 60% ethanol for 12 hours, measure and recover flavonoids;

[0021] Experimental results such as figure 1 As shown, the time of adding the adsorption resin has an impact on the yield of flavonoids. The addition of the resin too early will absorb a large amount of nutrients in the medium, which will affect the growth and metabolism of the bacteria; the addition of the resin is too late, and the adsorption efficiency is not high enough. , can not reach the effect of adsorbing flavonoids, releasing product repressi...

Embodiment 2

[0023] (1) Sterilize the DM130 macroporous adsorption resin, the sterilization condition is 121°C, 20min;

[0024] (2) Insert the Phellinus strain into the liquid fermentation medium, carry out shaker culture (28 ℃, 150rpm), when the culture time is 4 days, add 25, 50, 100 or 200g / 100ml of DM130 macroporous adsorption respectively Resin, then continue to cultivate for 3 days, and set blank control;

[0025] (3) filter to obtain resin after fermentation, desorb with 60% ethanol for 12 hours, measure and recover flavonoids;

[0026] Experimental results such as figure 2 As shown, the amount of adsorption resin has an impact on the yield of flavonoids. The addition of too much adsorption resin will affect the dissolved oxygen status in the medium, and the lack of oxygen supply will affect the growth and metabolism of the bacteria; The adsorption effect of flavonoids is not good, and the yield of flavonoids cannot be increased very effectively. Adding 50 g of DM130 macroporous...

Embodiment 3

[0028] (1) Sterilize the DM130 macroporous adsorption resin, the sterilization condition is 121°C, 20min;

[0029] (2) Insert the Phellinus phylloxera strain in the liquid fermentation medium, carry out shaker culture (28 ℃, 150rpm), when the cultivation time is 4 days, add the DM130 type macroporous adsorption resin of 50g / 100ml, then continue to cultivate for 3 days , and set a blank control;

[0030] (3) filter to obtain resin after fermentation, desorb with 60% ethanol for 12 hours, measure and recover flavonoids;

[0031] Experimental results such as image 3 As shown, in the two-phase culture experiment, the addition of DM130 macroporous adsorption resin can effectively adsorb flavonoids, relieve the repression of metabolite accumulation, and promote the secretion of flavonoids from Phellinus japonica. The flavonoid production can reach 2174.35 μg / ml, which is higher than that of the control. up about 35%.

[0032] Description of drawings

[0033] figure 1 Is the ...

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Abstract

The present invention relates to a process for producing flavone through liquid-solid double-phase culture of medicinal mushroom phellinus igniarius, and belongs to the technical field of biological fermentation engineering. According to the present invention, mainly during a phellinus igniarius liquid fermentation process, a macroporous absorption resin is added to a culture liquid to establish a liquid-solid double-phase culture system so as to improve a flavone yield; with the double-phase culture process, the flavone yield can be up to 2174.35 mug / ml and is increased by 35% compared with the control; and the process has characteristics of simple operation and strong generalization, and is especially suitable for large-scale higher fungi fermentation and active substance production.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation engineering. Background technique [0002] Phellinus igniarius belongs to Basidiomycotina, Hymenomycetes, Polyporales, Hymenochaetacae, Phellinus, is a kind of Higher fungi with high medicinal value. According to the records of "On the Properties of Medicine": Phellinus japonica is sweet and flat in nature, non-toxic, and can treat metrorrhagia, stranguria with blood, prolapse of the anus and diarrhea of ​​blood, leukorrhea, and amenorrhea. According to the analysis, the composition of Phellinus Phellinus is extremely complex. Polysaccharides and flavonoids are its main medicinal ingredients, which have anti-aging, lower cholesterol, protect the liver, inhibit tumors, promote the excretion of harmful substances and cholesterol, etc. In traditional Chinese medicine in my country It is also used for the treatment of diseases such as dysentery and night sweats. In addition, Phellinu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02C12R1/645
Inventor 朱虎刘伟刘建国曲剑波吕建仁
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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