Endophytic fungus Phomopsis sp. and use thereof

A technology of Phomopsis and endophytic fungi, which is applied in the fields of fungi and fermentation, and can solve the problems that the resources of plant endophytic fungi are not fully explored

Active Publication Date: 2011-08-17
ZHEJIANG DAYANG BIOTECH GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

A few species have been used in medicine, agronomic production or industrial applica

Method used

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  • Endophytic fungus Phomopsis sp. and use thereof
  • Endophytic fungus Phomopsis sp. and use thereof
  • Endophytic fungus Phomopsis sp. and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Example 1, Separation and purification of Phomopsis genus plant endophytic fungus new species ZJWCF252:

[0073] (1) Collection of plant tissue specimens: in Zhejiang Wencheng Shiyang Forest Farm (120°05E, 27°47′N, located in the northwest of Wencheng County, Zhejiang Province, at the junction of Wencheng, Jingning and Taishun, typical It has a mid-subtropical marine monsoon climate. There are 8 species of wild plants under national key protection, accounting for 72.7% of the total number of 11 species of wild plants under national key protection in Wencheng County, Zhejiang Province, including 8 species of timber trees, 4 species of medicinal plants, 8 species of ornamental plants, 2 kinds of oil plants and 5 kinds of scientific research plants), in mid-May 2007, plant specimens were collected at an altitude of 500-1000m, including Chinese goosebeery and Camptotheca acuminata, the medicinal plant fairy fruit of Moraceae (Ficus carica L.), the collection parts include r...

Embodiment 2

[0076] Embodiment 2, identification of plant endophytic fungus ZJWCF252

[0077] Identify through morphology and molecular systematics, use PDA medium and spore-stimulating medium, and use various spore-stimulating methods to cultivate, and perform colony shape, color, growth rate and microscopic morphological characteristics of the isolated endophytic fungi To observe, according to its sporulation structure, conidiophorus attachment, spore shape and color, etc., combined with ribosomal deoxyribonucleic acid internal transcription spacer (Internal transcribed spacer of ribosomal DNA, ITS rDNA), translation elongation factor ( translation elongation factor 1alpha, tef1), mating type (mating-type, MAT) DNA sequence for classification and identification. The endophytic fungus ZJWCF252 has the following microbiological characteristics:

[0078] The endophytic fungus ZJWCF252 grows very slowly in PDA medium, wort medium and spore-promoting medium. The diameter of the colony is onl...

Embodiment 3

[0083] Embodiment 3, endophytic fungus screening with bacteriostatic activity

[0084] Use the two-point confrontation culture method to screen the active strains, cut the purified endophytic fungus cake with a stainless steel puncher with a diameter of 5mm, insert it into one side of a PDA plate with a diameter of 90mm (30mm from the center), and culture at 25°C After 2 days, inoculate the pathogenic indicator bacteria on the other side of the PDA plate (30mm from the center), and at the same time, use only the disease indicator bacteria as a control. After 2-5 days, observe the growth and antibacterial conditions of the colonies, and measure the endophytic fungi and pathogenic bacteria with a caliper Relative radius of colony growth: r 内 、r 病 , and the control radius r 0 , After the pathogenic bacteria intersect with the endophyte colony, observe and record the process of the endophytic fungus encircling, inhibiting and covering the pathogenic bacteria colony to occupy its...

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Abstract

The invention discloses an endophytic fungus Phomopsis sp. strain ZJWCF252, which was collected with a name of Phomopsiswenchengensis and a collection number of CGMCCNo.4301 in China General Microbiological Culture Collection Center of Institute of Microbiology of Chinese Academy of Sciences, which is located in building No.3, Yard No.1, Beichen Road, Chaoyang District, Beijing, on November 1st, 2010. The invention also discloses the use of the endophytic fungus Phomopsis sp. in the preparation of 2,3-dihydro-2-hydroxy-2,4-dimethyl-5-trans-propenylfuran-3-one which has strong inhibition effects on various plant pathogenic fungi.

Description

technical field [0001] The present invention relates to the field of microbiology and biomedicine technology, in particular, the present invention relates to a new Phomopsis plant endophytic fungus ZJWCF252 (Phomopsis wenchengensis) and its antifungal compound 2,3-dihydro-2 -hydroxy-2,4-dimethyl-5-trans-propenylfuran-3-one, and the use of this strain to prepare 2,3-dihydro-2-hydroxy-2,4-dimethyl-5-trans-propenylfuran -3-one approach. Background technique [0002] For a long time, irrational drug use or even blind drug use has promoted the rapid emergence of bacterial resistance, resulting in the fact that many old types of drugs have little application value now, and it is increasingly important to find and develop new active compounds. Due to the narrow range of sources of botanical medicines and the increasing demand for medicines in recent years, many precious medicinal plants have been developed out of order, leading to the collapse of wild plant resources, and even a v...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P17/04
Inventor 王国平章初龙林福呈夏静周转忠
Owner ZHEJIANG DAYANG BIOTECH GROUP
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