Production process of flavonoids by liquid-solid two-phase culture of Phellinus japonica

A technology of phellinus and flavonoids, applied in the field of biological fermentation engineering, to achieve the effect of simple cultivation process and increased yield

Inactive Publication Date: 2016-01-27
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method of utilizing liquid-solid two-phase culture to improve Phellinus flavone production has not been reported in the literature, so it has a good development prospect

Method used

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  • Production process of flavonoids by liquid-solid two-phase culture of Phellinus japonica
  • Production process of flavonoids by liquid-solid two-phase culture of Phellinus japonica
  • Production process of flavonoids by liquid-solid two-phase culture of Phellinus japonica

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] (1) Sterilize the DM130 macroporous adsorption resin, and the sterilization conditions are 121°C, 20min;

[0019] (2) Insert Phellinus linteus strain into the liquid fermentation medium, carry out shaker culture (28°C, 150rpm), when the culture time is 3, 4, 5 or 6 days, add 50g / 100ml DM130 macroporous adsorption resin, and then continue to cultivate, and set a blank control;

[0020] (3) after fermenting for 7 days, filter to obtain resin, desorb with 60% ethanol for 12 hours, measure and recover flavonoids;

[0021] The experimental results are as figure 1 As shown, the addition time of the adsorption resin has an impact on the yield of flavonoids. The premature addition of the resin will absorb a large amount of nutrients in the medium, which will affect the growth and metabolism of the bacterial cells; if the resin is added too late, the adsorption efficiency is not high enough. , can not achieve the effect of adsorbing flavonoids, releasing product inhibition and...

Embodiment 2

[0023] (1) Sterilize the DM130 macroporous adsorption resin, and the sterilization conditions are 121°C, 20min;

[0024] (2) Insert Phellinus linteus strain into the liquid fermentation medium, carry out shaker culture (28°C, 150rpm), when the culture time is 4 days, add 25, 50, 100 or 200g / 100ml of DM130 macroporous adsorption respectively resin, and then continue to cultivate for 3 days, and set a blank control;

[0025] (3) filter to obtain resin after fermentation finishes, desorb with 60% ethanol for 12 hours, measure and reclaim flavonoids;

[0026] The experimental results are as figure 2 As shown, the amount of adsorption resin added has an impact on the yield of flavonoids. The addition of too much adsorption resin will affect the dissolved oxygen status in the medium, and insufficient oxygen supply will affect the growth and metabolism of bacteria; The adsorption effect of flavonoids is not good, and the yield of flavonoids cannot be improved very effectively. Ad...

Embodiment 3

[0028] (1) Sterilize the DM130 macroporous adsorption resin, and the sterilization conditions are 121°C, 20min;

[0029] (2) insert Phellinus linteus strain in the liquid fermentation medium, carry out shaking table culture (28 ° C, 150rpm), when the culture time is 4 days, add 50g / 100ml DM130 type macroporous adsorption resin, and then continue to culture for 3 days , and set a blank control;

[0030] (3) filter to obtain resin after fermentation finishes, desorb with 60% ethanol for 12 hours, measure and reclaim flavonoids;

[0031] The experimental results are as image 3 In the two-phase culture experiment, adding DM130 macroporous adsorption resin can effectively adsorb flavonoids, relieve the inhibitory effect of metabolite accumulation, and promote Phellinus linteus to secrete flavonoids. about 35%.

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Abstract

The present invention relates to a process for producing flavone through liquid-solid double-phase culture of medicinal mushroom phellinus igniarius, and belongs to the technical field of biological fermentation engineering. According to the present invention, mainly during a phellinus igniarius liquid fermentation process, a macroporous absorption resin is added to a culture liquid to establish a liquid-solid double-phase culture system so as to improve a flavone yield; with the double-phase culture process, the flavone yield can be up to 2174.35 mug / ml and is increased by 35% compared with the control; and the process has characteristics of simple operation and strong generalization, and is especially suitable for large-scale higher fungi fermentation and active substance production.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation engineering. Background technique [0002] Phellinus signiarius belongs to Basidiomycotina, Hymenomycetes, Polyporales, Hymenochaetacae, Phellinus, and is a kind of medicine. Use highly valuable higher fungi. According to "Medicinal Properties" records: Phellinus linteus is sweet and flat, non-toxic, cures metrorrhagia, blood stranguria, prolapse of the rectum, diarrhea, vaginal discharge, and amenorrhea. According to the analysis, the composition of Phellinus linteus is extremely complex. Polysaccharides and flavonoids are its main medicinal components, which have the functions of anti-aging, lowering cholesterol, protecting the liver, inhibiting tumors, promoting the excretion of harmful substances and cholesterol, etc. It is also used for the treatment of dysentery, night sweats and other diseases. In addition, Phellinus linteus extract has anti-cancer efficacy, and has playe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/02C12R1/645
Inventor 朱虎刘伟刘建国曲剑波吕建仁
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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