40 results about "Mytilus" patented technology

The present invention discloses a preparation method and an application of a mussel oligopeptide, and belongs to the field of marine biotechnology application. The preparation method comprises: adopting mytilus edulis as a raw material, and adopting an alkaline cold extraction method to obtain mussel protein from mussel freeze-drying powder; and carrying out composite enzymolysis of protein to obtain mixed polypeptides, carrying out ultra-filtration to obtain the oligopeptide, adopting aging mouse models to carry out a series of anti-aging animal experiments and behavioral experiments, and carrying out a drosophila survive experiment to prove aging delay activity of the mussel oligopeptide so as to create a new approach for achievement of scientific and high-value mussel utilization. Compared with the method in the prior art, the method of the present invention has the following advantages that: the mussel protein is extracted and prepared, the optimal protease and the preferred process are adopted to carry out enzymolysis purification on the mussel protein, decomposition on the mussel protein molecule structure due to the high temperature is avoided, the grading of the mussel polypeptide at the molecular weight level is achieved, and the obtained mussel oligopeptide has the pure and good aging delay effect.

The invention provides an automatic processing production line for Mytilus coruscus, belongs to the technical field of fishery tools, and solves the technical problems of high labor intensity and lowefficiency in existing Mytilus processing. The automatic processing production line for the Mytilus coruscus comprises a conveyor belt and a driving motor driving the conveyor belt to operate, whereinan inclined directional conveying device is arranged on the side, close to an input end, of the conveyor belt; multiple fixing boxes capable of accommodating and fixing the Mytilus coruscus are placed on the conveyor belt at intervals, a meat removing device is arranged on the side, close to an output end, of the conveyor belt, a shell opening device is also arranged between the directional conveying device and the meat removing device, the shell opening device comprises a shell opening tool and a cut-off tool which are arranged vertically downwards, and the shell opening tool and the cut-offtool are arranged in the width direction of the conveyor belt side by side at an interval; and the meat removing device comprises a jet nozzle for jetting high-pressure water flows. The automatic processing production line is high in automation degree and high in work efficiency.

The invention belongs to the field of marine biological component extraction, and relates to a preparation method of a mollusc antiallergic extract and application of the mollusc antiallergic extractto cosmetics, in particular to a preparation method of a mussel antiallergic peptide and application of the mussel antiallergic peptide to the cosmetics, and application of molluscs, especially the mussel antiallergic extract as an effective component to preparation of medicines, skin-care products or/and the cosmetics. The preparation method comprises the steps of pretreatment, homogenization, ultra-high-pressure extraction, subcritical extraction, enzymolysis and ultrafiltration, and can further comprise the steps of vacuum concentration and drying. A normal-temperature ultra-high-pressure technology and a subcritical extraction technology are combined to be applied to preparation of the mussel antiallergic extract, the produced extract can have the characteristics that the extraction efficiency is high, the component activity is high, chain scission is uniform, and the molecular weight can be controlled within 3KD, and the content of small peptides in mussels can reach upmost to 89.79%; and the mussel extract has the excellent antiallergic capacity and the high anti-oxidation effect.

The invention discloses a mytilus coruscus multi-drug resistance protein Abcc. The multi-drug resistance protein Abcc is a cloned gene, which is obtained by cloning a gene core fragment of mytilus coruscus Abcc by a gene cloning technology. The invention also discloses a novel marine biological pollution detection marker, which uses mytilus coruscus as a biological sample for detecting marine pollution, and uses the mytilus coruscus multi-drug resistance protein Abcc as a detection marker for marine pollution. The relative expression amount of a multi-drug resistance protein Abcc gene of the mytilus coruscus multi-drug resistance protein Abcc is the highest in digestive glands and gills, and a PCR reaction system for cloning can ensure the specificity of an amplification reaction and improve the PCR amplification efficiency. The detection marker provided by the invention has strong specificity, can directly take target cells or target molecules in organisms as reaction endpoints to carry out sensitive biological reactions at the molecular level, and has an early warning effect on marine pollutant exposure and toxicity effects.

The invention relates to a usage of potassium ions in promoting the metamorphosis of mytilus coruscus larvae and shortening the seed settlement time of the mytilus coruscus larvae and further relates to a usage of a potassium ion antagonist in inhibiting the metamorphosis of the mytilus coruscus larvae and a method for promoting or inhibiting the metamorphosis of the mytilus coruscus larvae. The usage and the method have the advantages that evidences prove that by the potassium ions, the metamorphosis rate of the mytilus coruscus larvae can be improved, and the seed settlement time of the mytilus coruscus larvae can be shortened, so that the potassium ions can be used for increasing the production success rate of mytilus coruscus seeds and shortening the production period; and meanwhile, cheap and nontoxic sylvite is suitable for large-scale seed production. The evidences also prove that the potassium ion antagonist can inhibit the metamorphosis of the mytilus coruscus larvae, and a novel way is provided to control marine contamination. The conclusion of the study is helpful in stating the settlement and metamorphosis mechanism of mytilus coruscus.

The invention relates to an application of ethanol to accelerating mytilus coruscus larva metamorphosis and/or shortening spatfall time of mytilus coruscus larvae, and further relates to a method of improving a mytilus coruscus larva metamorphosis rate or shortening a mytilus coruscus cultivating period. The method comprises the following steps of putting the mytilus coruscus larvae into an ethanol solution with the concentration of 0.1-1 M to carry out exposure treatment for 1-24 hours; and then transferring into a utensil containing seawater to cultivate. According to the application and the method disclosed by the invention, the ethanol can obviously improve the mytilus coruscus larva metamorphosis rate and shorten the mytilus coruscus cultivating period and the cost of the ethanol is low, so that the ethanol has a wide application prospect in a mytilus coruscus cultivation industry; and the application further provides suitable dosage, treating time and induction time when the ethanol is used as an inductor to improve the mytilus coruscus larva metamorphosis rate; and under the condition, the induction efficiency is high, the effect is stable and specifications of obtained young shellfishes are the same, so as to provide guidance to production practices.

The invention provides a method for repairing a seaweed field by culturing a shellfish-algae complex. The method comprises the following steps of collecting wild species algae before sargassum vachellii and sargassum horneri are mature, temporarily culturing the wild species algae in an outdoor water tank, and placing an oxygenation pump for temporary culture; washing the seed algae with flowing water, collecting the sargassum horneri and sargassum vachellii juvenile sporophytes, and moving to a collecting barrel; evenly laying mytilus coruscus at the bottom of the collecting barrel, smearing spore liquid on the surface of the mytilus coruscus through a brush, slightly adding seawater along the barrel wall, and placing the oxygenation pump outdoors for the temporary culture; after growing the sargassum horneri and the sargassum vachellii to 5-10 centimeters on the surfaces of the mytilus coruscus, separating the piled mytilus coruscus one by one, and putting the shellfish-algae complex into an algae field to restore a sea area. According to the method, attachment of the juvenile sporophytes is completed in the culture pond firstly, then secondary attachment is rapidly achieved with the help of the mytilus coruscus, the influence of sediments and illumination intensity on growth of the juvenile sporophytes can be effectively avoided, and the finally-grown shellfish-algae complex can better repair the algae field repair sea area.

The invention discloses application of oyster peptide in promoting marine shewanella to induce juvenile mytilus coruscus to adhere. The marine shewanella is preserved in the China General Microbiological Culture Collection Center, the preservation number is CGMCC No: 9798, the concentration of the oyster peptide is 0.01-1 mg/mL, and the oyster peptide is oyster oligopeptide with the oligopeptide mass percentage content not lower than 65% and the molecular weight smaller than 1 kDa. The oyster peptide has the promoting effect on inducing juvenile mytilus coruscus to adhere through the marine shewanella, aquaculture of mytilus and stability of mytilus populations in the ocean are facilitated, and the oyster peptide has important significance in exploring the juvenile mytilus coruscus adhering mechanism and cultivating and breeding of mytilus coruscus.

The invention provides use of sulfitobacter sp. in inducing adhesion of young mytilus coruscus. Sulfitobacter sp.G213-c has the preservation number of CCTCC NO:M 2019806. The invention discloses use of a microbial film formed by the sulfitobacter sp. in inducing adhesion of young mytilus coruscus. An induction process comprises the following steps: separating and purifying sulfitobacter sp. to obtain purebred sulfitobacter sp.; carrying out inoculated culture on the purebred sulfitobacter sp., and precipitating and washing to obtain a suspended bacteria solution; and adding sterilized seawaterfor culturing to obtain a microbial film and performing an induction culture on glass slides attached with the microbial film and the young mytilus coruscus. Therefore, the microbial film formed by the sulfitobacter sp. can induce adhesion of young mytilus coruscus, an induction activity of the microbial film with different bacterium initial densities on the young mytilus coruscus is different, and the sulfitobacterr sp. has important significance on artificial culture of the mytilus coruscus.

The invention discloses deep-sea roseobacter and application of the deep-sea roseobacter in induction of attachment of mytilus coruscus, and belongs to the technical field of marine microorganisms. The deep-sea roseobacter is named as deep-sea roseobacter Roseovarius nubinhibens G213-d, is preserved in China Center for Type Culture Collection (CCTCC), has a preservation number of CCTCC NO: M 2019807, has a 16S rRNA gene as shown in SEQ ID NO: 1, and is derived from ocean sediments in sea areas near South China Sea cold spring at the altitude of -1151.16 meters. According to the invention, Roseudomonas nubinhibens G213-d is obtained through separation, purification and identification, and meanwhile, it is verified that a biofilm formed by the Roseobacterium nubinhibens G213-d has activity of inducing attachment of mytilus coruscus, so that a new technical approach is provided for seedlings and induction of attachment of mytilus coruscus by using the biofilm formed by the bacteria.