17 results about "Mytilus" patented technology

The invention discloses a bionic cross-linked film-forming repair dressing containing mussel mucoprotein and a preparation method of the bionic cross-linked film-forming repair dressing, and relates to the technical field of medical instruments. The bionic crosslinking film-forming repair dressing containing the mussel mucoprotein is prepared from the following components in parts by weight: 30 to 40 parts of bionic crosslinking agent, 25 to 35 parts of humectant, 10 to 20 parts of natural high polymer material, 1 to 10 parts of mussel mucoprotein, 1000 parts of purified water and 5 to 15 parts of pH regulator. PEG-DOPA is formed through cross-linking, the DOPA content and stability are remarkably improved, the adhesion-repair-anti-inflammatory effect of mussels mainly depends on the dopa content, the effect of the dressing is remarkably enhanced through improvement of the DOPA content and stability, the dressing is more convenient to use through the flexibility change of a film, the dressing is more suitable for dynamic and wet wounds, and the application range of the dressing is widened. The medicine has a remarkable effect on chronic ulcer and burn.

The invention belongs to the technical field of gene intervention, and provides a feeding-mediated marine mussel gene silencing method which comprises the following steps: linearizing plasmids to obtain linearized plasmids; determining a cDNA fragment of the target gene from the target mussel, and performing amplification by using the specific primer pair to obtain a target gene fragment; connecting the target gene segment with the linearized plasmid to obtain a connection product; transforming the connection product into escherichia coli, screening to obtain a positive strain, extracting target recombinant plasmids, and transforming the target recombinant plasmids into escherichia coli for expressing dsRNA to obtain engineering bacteria; engineering bacteria and microalgae are mixed according to the cell number ratio of (90-110): (1-2), a bacteria-algae feeding mixture is obtained, and mussels are fed with artificial seawater containing the bacteria-algae feeding mixture for 1-10 d. The method disclosed by the invention does not cause antioxidant system disorder or energy metabolism abnormality, and is a gene silencing strategy with higher biological safety.

The invention is applicable to the technical field of biopharmacy process, and provides a freeze-drying method for improving the bioactivity of recombinant mussel mucin, which comprises the steps of pretreatment, pre-freezing, primary drying and secondary drying. In the pretreatment step, a recombinant mussel mucoprotein solution with the pH value of 3.5-5.5 is mixed with a composite protective agent composed of trehalose, L-arginine and poloxamer according to a specific proportion; in the pre-freezing process, slow-fast-slow sectional cooling is adopted; drying adopts a vacuum drying mode combining gradient heating and gradient vacuum degree; according to the invention, creative coupling of the protective agent and the process is realized, the retention rate of the prepared product DOPA is more than 90%, the residual moisture is lower than 1.5%, the redissolution time is shorter than 30 seconds, the microstructure is excellent, and the long-term storage stability is good.

The invention relates to a common mussel umami peptide as well as a preparation method and application thereof, and belongs to the technical field of food processing. The invention provides a method for preparing umami peptide from common mussels aiming at the problems that the existing common mussel processing mode is low in additional value and lacks in deep understanding of umami components of the common mussels, the common mussels are used as raw materials, after homogenate, flavourzyme is used for enzymolysis, then an enzymolysis product is separated and purified through gel column chromatography, and the umami peptide is obtained. Specifically, the elution process is monitored under the wavelength of 210 nm, and target elution components are collected according to a peak appearance sequence, so that the mytilus edulis umami peptide with strong umami is obtained. The mytilus edulis umami peptide prepared by the invention is pure in flavor, natural and safe, and can be applied to food seasoning.

This invention discloses a method for hydroxylation modification of recombinant mussel foot protein and its application. The invention utilizes a whole-cell catalyst with tyrosinase catalytic activity to hydroxylate tyrosine residues in recombinant mussel foot protein. This method eliminates the need for cell disruption and protein purification steps. By immobilizing tyrosinase on the host cell surface through cell surface display, the recombinant mussel foot protein can directly contact the tyrosinase in the extracellular environment and undergo hydroxylation, thereby introducing dopamine groups into the protein to improve its underwater adhesion properties. This method is applicable to the hydroxylation modification of various types of recombinant mussel foot protein and has advantages such as high catalytic efficiency, good stability, simple operation, and low cost, making it valuable for industrial applications.

The application discloses a mussel polysaccharide and a preparation method and application thereof. The mussel polysaccharide MP-I with a novel structure is prepared from Mytilus coruscus through the process steps of water extraction and concentration, enzymatic deproteinization, alcohol precipitation and anion exchange, and the like. Animal experiments prove that the mussel polysaccharide MP-I prepared by the application can effectively improve intestinal flora and relieve intestinal inflammation by inhibiting the expression of inflammatory signal pathways such as JAK2 / STAT3 and NF-kappa B. Therefore, the mussel polysaccharide MP-I is a natural and safe drug or food for improving inflammatory bowel disease, can effectively improve the inflammatory bowel disease, is safe, non-toxic and free of side effects, the raw material is abundant, the steps are simple, the cost is low, and the effect is remarkable.

The invention relates to a recombinant mussel mucin MFP535. The invention further provides a coding nucleic acid, a recombinant vector, a recombinant strain and the like for highly expressing the MFP535, so that the efficient expression of the recombinant mussel mucin MFP535 is realized. The invention further provides a purification and separation method for the recombinant mussel mucin MFP535, the purity of the MFP535 finally obtained through purification reaches 96% or above, the tyrosine oxidation rate reaches 0.44%, and the endotoxin is lt; 1 EU / mg. The recombinant mussel mucin MFP535 has an obvious effect of promoting cell growth. The separation and purification method disclosed by the invention is high in specificity, mild in operation condition, simple in step, good in purification effect, high in recovery rate, low in cost, high in speed and beneficial to expanded production.

The application provides an aqueous UV insulating coating, a preparation method and application thereof. The application controls the preparation method to obtain a star-shaped epoxy soybean oil prepolymer, introduces an acrylate, a siloxane chain segment and a mussel myoalbumin into the prepolymer with the star-shaped structure to obtain an insulating resin matrix, improves the electrical property, heat resistance, mechanical strength and adhesion to the substrate of the coating resin after curing of the coating, and further introduces a phosphoric acid group to react with the mussel myoalbumin to generate a mussel phosphate, so that the adhesion to the substrate of the coating can be significantly improved.

1. The name of the design product: packaging kit (medical recombinant mussel mucin care cream). 2. The use of the design product: for product packaging. 3. The design points of the design product: in the pattern. 4. The picture or photo that best indicates the design points: main view of kit 2. 5. Other circumstances that need to be explained: The design product is a kit product, so it is used and sold as a set. Kit 1 is a packaging bottle, kit 2 is a packaging box, and kit 1 is packed into kit 2 for sale and use.

The invention discloses a mussel adhesion spot matrix protein peptide as well as a screening method and application thereof, and belongs to the technical field of bioactive peptides. The peptide sequences of the mussel adhesion spot matrix protein peptide are KPSYTP, MEGIK and SYPQA; the screening method specifically comprises the following steps: (1) virtual enzymolysis of mussel adhesion spot matrix protein; (2) screening the bioactive peptide; and (3) molecular docking. Three bifunctional peptides KPSYTP, MEGIK and SYPQA which are high in activity, high in safety, easy to absorb and brand-new in structure are excavated from mussel adhesion spot matrix protein, can cope with two major pathological links of oxidative stress and cholinergic system dysfunction at the same time, and have unique advantages in the aspect of prevention or auxiliary intervention of neurodegenerative diseases such as Alzheimer's disease and the like; not only can a marine active peptide library be enriched, but also a new solution is provided for developing functional foods, special medical foods or natural raw materials for assisting in intervening neurodegenerative diseases.

The utility model belongs to the technical field of mytilus coruscus spat separation cultivation, and particularly relates to a mytilus coruscus spat separation attachment device which comprises a loading unit which comprises a bottom plate, a temporary storage bin, supporting legs, a connecting arc pipe and an electromagnetic flow valve and is used for temporarily storing separated mytilus coruscus spats. According to the mussel seedling attaching device, the loading unit, the guiding unit and the attaching unit are used in cooperation, mussel seedlings are evenly distributed in the bag body and effectively attached to the outer surface of the elastic brush, the bag body can be rapidly detached only in the direction of the guiding groove, the attaching efficiency of the mussel seedlings is improved, and the labor intensity of workers is reduced. Through the combination of the degradable jute bag body and the elastic brush, traditional plastic pollution is avoided, a bionic attachment surface is formed, and the attachment rate of the young mussels is remarkably increased; the PVC pipe is driven to incline through the inclining unit, the PVC pipe inclines to drive the bag body to incline, and therefore water in the bag body is separated, the bag body is prevented from being degraded too early, and the practicability and applicability of the device are further improved.

The application discloses a mussel mucin polypeptide or a composition thereof, a preparation method and application. The amino acid sequences of the mussel mucin polypeptide are respectively shown as SEQ ID NO. 1, SEQ ID NO. 2, SEQ ID NO. 4, SEQ ID NO. 6, SEQ ID NO. 7, SEQ ID NO. 12, SEQ ID NO. 14, SEQ ID NO. 16, SEQ ID NO. 20, SEQ ID NO. 21, SEQ ID NO. 22, SEQ ID NO. 25, SEQ ID NO. 27 or SEQ ID NO. 31. The polypeptide component prepared by the application can be used for preparing a medicine or food for improving inflammatory bowel disease. The mussel mucin polypeptide can effectively improve the inflammatory bowel disease, is safe, non-toxic and free of side effects, simple in steps and remarkable in effect.

The invention belongs to the technical field of biological medicines, and particularly relates to a recombinant mussel mucin dressing for promoting wound healing and a preparation method thereof. The recombinant mussel mucoprotein dressing is prepared from the following raw materials in parts by weight: 1.2 to 2.5 parts of recombinant mussel mucoprotein, 0.5 to 1 part of phellinus igniarius polypeptide, 5 to 10 parts of chitosan, 15 to 20 parts of sodium alginate, 3 to 8 parts of glycerol, 5 to 10 parts of propylene glycol and 63 to 72 parts of deionized water. The recombined mussel mucin Mfp-r has a synergistic effect with phellinus igniarius polypeptide, chitosan and sodium alginate in the dressing, wound infection can be quickly controlled, wound healing time can be shortened, hyperplasia of scars can be inhibited, healing quality can be remarkably improved, and the recombined mussel mucin Mfp-r is a wound repairing dressing with great clinical application prospects.

This invention discloses a method for modifying the structure of recombinant mussel adhesive protein, comprising the following steps: S1. Optimizing the mussel adhesive protein gene sequence and cloning it into an expression vector to construct a recombinant plasmid; S2. Transforming the recombinant plasmid into competent cells and culturing them to obtain engineered bacteria; S3. Fermenting the engineered bacteria, collecting the bacterial cells by centrifugation, adding a lysis solution to break the bacterial cells, purifying and collecting the eluent, and dialysis to obtain a dialysate containing non-adhesive recombinant mussel adhesive protein; S4. Adding a chemical oxidant and an enhancer to the dialysate containing the non-adhesive recombinant mussel adhesive protein to react and obtain a highly adhesive recombinant mussel adhesive protein. This invention provides a novel chemical structure modification method for recombinant mussel adhesive protein. Through the synergistic effect of highly selective oxidants and enhancers, the tertiary structure of the protein is unfolded and the reaction activation energy is reduced, achieving efficient and simultaneous tyrosine hydroxylation and DOPA quinone crosslinking. The modification rate is more than 7 times higher than that of traditional chemical methods.

This invention discloses a production quality control method for medical mussel adhesive protein nasal spray, belonging to the field of medical preparation production and quality management technology. The method acquires measured data on the physicochemical parameters of each process in historical batches, as well as the molecular aggregation state spectrum and oxidative crosslinking activity state of the finished product, and constructs a two-way control sample set corresponding to each batch. A multivariate gradient coupling fitting algorithm is used to construct a mapping model between physicochemical parameters and functional states. Real-time physicochemical parameters of the current production batch are input, and a hidden state time-series extrapolation algorithm is used to output dynamic evolution data and determine the quality level. Data from multiple batches are integrated to form a batch quality traceability chain. For batches with abnormal deviations, the production process is adjusted and the extrapolation is repeated until the functional state deviation converges. This invention achieves continuous monitoring and dynamic quality management of the production process of medical mussel adhesive protein nasal spray by mapping physicochemical parameters to functional states and combining time-series extrapolation and batch traceability.

The invention relates to the technical field of biology, in particular to mytilus coruscus exosome-like nano-vesicles and application thereof in preparation of osteogenesis products. The functional activity research system of the exosome-like nano vesicles from aquatic products can be improved in the academic level, meanwhile, novel safe and efficient active substances are explored for the bone health field, high-value utilization of shellfish resources can be promoted, the academic value is remarkable, and the method has important industrial application prospects.

The invention belongs to the technical field of aquatic food safety detection, and particularly relates to a common mussel heavy metal pollution detection method and system based on CNN and femtosecond laser-induced breakdown spectroscopy, the method is realized based on an Fs-LA-LIBS spectrum detection device, and the method comprises the following steps: preparing common mussels to be detected into a sample, and placing the sample on a three-dimensional translation table of the Fs-LA-LIBS spectrum detection device; the method comprises the following steps: carrying out spectrum acquisition and pretreatment on a sample based on an Fs-LA-LIBS spectrum detection device to obtain statistical characteristics; based on a CNN network model, constructing a common mussel heavy metal pollution detection model; and based on the statistical characteristics and a common mussel heavy metal pollution detection model, obtaining a common mussel heavy metal pollution detection result. Through the CNN and femtosecond laser-induced breakdown spectroscopy embedded collaborative architecture, the three technical problems existing in common mussel heavy metal detection by a traditional method are solved, and non-destructive, high-precision and online quantitative detection is realized.