30 results about "Mussel adhesion" patented technology

The invention provides a method for preparing a marine mussel adhesive protein waterproof biological adhesive, and relates to a biological adhesive. The invention provides the method for preparing marine mussel-Mytilus coruscus adhesive protein waterproof biological adhesive with degradability, water resistance and good biological compatibility by a method of biological gene engineering. The method comprises the following steps: extracting total RNA from the foot part of alive Mytilus coruscus to be reversely transcribed into cDNA; designing a PCR primer according to an Mcfp-1 gene sequence of the Mytilus coruscus; connecting the obtained DNA fragment in a prokaryotic expression vector pGEX-4T through PCR amplification to construct a recombinant plasmid; then transforming the recombined plasmid in a colon bacillus BL21 bacterial strain; and finally, purifying and modifying the expressed polypeptide product. The preparation method is simple, and the expression product is soluble, can be easily purified with low cost, and has similar or better effect compared with CELL-TAK<TM> cell and tissue adhesive without cytotoxicity. The adhesive accords with the requirement of scientific research, and can be used as a biological medical material.

The invention discloses a catechol Mytilopsis-adhered and amphion-like extracellular membrane structured double-bionic modified multi-armed PEG as shown in the general structural formula (I) and a preparation method thereof. By a simple direct coating mode such as dispensing, spraying, dip-coating or spin-coating, etc. of the PEG, a multi-armed polyethylene glycol flexible chain and amphion-like extracellular membrane structured double anti-biopollution surface is constructed on the surface of various polydopamine-mediated base materials. Due to a branching structure and multiple modifiable ends of the bionic modified multi-armed PEG, it brings the possibility to prepare a functional polymer with multiple functional groups and regulable proportion. As the polydopamine with ultrastrong adhesion is used as a mediation layer, a compact and stable double anti-biopollution coating can still be formed on the surface of almost any base materials on the condition that content of catechol in the multifunctional multi-armed PEG is limited.

The present invention relates to a body-type high-strength bionic adhesive preparation method, which comprises: adding polyvinyl alcohol and a solvent to a reaction kettle, adding a catalyst and a pyrocatechol-containing compound under protection of argon or nitrogen, carrying out a stirring reaction for a certain time at a temperature of 50-100 DEG C, cooling the system to a room temperature, precipitating in water, repeatedly washing the precipitate by using water, and carrying out vacuum drying to obtain the bionic adhesive. According to the present invention, the adhesive is synthesized by using the one-step method, such that the operation scale-up and the industrial production are easily achieved; the adhesive is the body-type adhesive, the curing is rapid, and the strength achieves more than or equal to 6 MPa; the adhesive has the wide substrate universality, and is suitable for adhesion of metals, plastics, glasses, ceramics, bones and the like, and is further suitable for preparation of formaldehyde-free wood-based plates; and the perfect combination of the synthetic polymer and the natural mussel adhesion protein is achieved, the technical bottleneck of the current bionic mussel adhesive is expected to be broken through, and the new approach is provided for the development of the bionic polymer materials based on shellfish.

The invention provides a method for preparing biological adhesive by using perna viridis to adhere protein, relates to a biological adhesive. The invention provides a method for preparing a waterproof biological adhesive with better biological compatibility and low cost that extracts adhesive protein from perna viridis having wide raw material and low cost. The method includes steps as follows: cutting foot from live perna viridis, then mincing and homogenating, processing protein acidification, acetone protein precipitation, purification, finally, obtaining the waterproof biological adhesive. The adhesive protein waterproof biological adhesive can be obtained rapidly from perna viridis most abundant in China southeast seaside. The perna viridis biological adhesive has characteristics of high production, low cost and better adhesive effect. The prepared adhesive can adhere on surface of glass, metallic titanium and plastic, adheres cell in short time, adheres plastic or mouse thighbone, and has no cell toxicity. The biological adhesive as biological medical material biological adhesive has wide application prospect in clinical use.

The present invention relates to a pH-responsive metal-catechol derivative nanoparticle for drug delivery based on a mussel adhesive protein, particularly Fe(III)-DOPA nanoparticle, and a method for preparing the same. A nanoparticle for drug delivery according to the present invention is prepared using a pH responsive substance, catechol derivative-metal complex, particularly Fe(III)-DOPA complex, based on a mussel adhesive protein. Thereby, the present invention has excellent biocompatibility, is capable of easily and quickly penetrating into a target cell, and releases a loaded drug under acidic conditions, which enables to selectively deliver a drug to a specific disease having an acidic environment.

The present invention relates to a coacervate comprising a mussel adhesive protein and an anionic polymer, and more particularly, to a coacervate prepared by mixing a mussel adhesive protein with an anionic polymer, and a novel use thereof. In the present invention, a coacervate prepared by mixing a mussel adhesive protein and an anionic polymer shows a very excellent adhesive strength to various substrates such as cells or metals, and is able to maintain its adhesive strength in the presence of water or under water, thereby being effectively used as an adhesive. Moreover, it has an activity capable of encapsulating bioactive materials, thereby being effectively used as an active component of a composition for delivering bioactive materials.

The invention discloses an antitumor slow-release implant having strong adhesion and hemostasis functions and a preparation method thereof. Inspired by mussel adhesion mechanism and catechol and iron ion chelating mechanism, postoperative thermal chemotherapy hydrogel based on mussel adhesion mechanism deriving is developed successfully through bionic design and serves as a multifunctional operative tissue adhesion and treatment agent. Main ingredients of the implant include chitosan, gallic acid, Fe3+ chelating agent and drug. Coating and treating of a wound can be performed by covalently crosslinking and freeze-drying chitosan and gallic acid, dissolving before use, adding antitumor drug, utilizing the Fe3+ chelating agent for crosslinking and ultrasonically mixing well to form gel. The implant has various functions of adhesion, hemostasis, bacteria resisting, inflammation diminishing and thermal chemotherapy of the wound, and postoperative relapse prevention of tumor can be realized by coating an irregular wound of the tumor with the implant and performing mild thermal chemotherapy.

The invention discloses a preparation method of PEGDA-mussel adhesive protein-collagen composite hydrogel with strong adhesion and high mechanical strength, belonging to the technical field of hydrogel. In order to improve the mechanical strength and tissue adhesion of a collagen hydrogel, the invention discloses the preparation method for the PEGDA-mussel adhesive protein-collagen composite hydrogel with strong adhesion and high mechanical strength. The method comprises the following steps: dissolving mussel adhesive protein into an acetic acid solution to obtain an acetic acid solution of the mussel adhesive protein; dissolving a collagen solid in the acetic acid solution of the mussel adhesive protein to obtain a mussel adhesive protein-collagen solution; carrying out alkali neutralization until a pH value is in a range of 5-9, and adding a PEGDA solid until the solid is completely dissolved; and adding a photoinitiation factor, and carrying out ultraviolet crosslinking to form thePEGDA-mussel adhesive protein-collagen composite hydrogel. The preparation method is simple in process and easy to operate, and has wide application prospects in the fields of tissue engineering, medicine and the like.

The invention discloses MRRP protein, a gene, a carrier, engineering bacteria and application of the MRRP protein, the gene, the carrier and the engineering bacteria to preparation of wound healing medicine. The MRRP protein is formed by sequentially connecting mussel attachment protein Ma1, elastic drosophila melanogaster protein Res1, a spider silk protein nitrogen end sequence Nt1, a spider silk protein middle-piece repetitive sequence SSR, a spider silk protein carbon end sequence Ct1, elastic drosophila melanogaster protein Res2 and mussel attachment protein Ma2. The MRRP protein integrates self-assembling performance of spider silk protein, high elasticity of the elastic drosophila melanogaster protein and high viscosity of the mussel attachment protein, comprehensive performance is excellent, lengths are uniform, the MRRP protein can be biodegraded completely, the yield is high by adopting a microorganism method for preparing, and chemical pollution is avoided; the MRRP protein has high elasticity, and can bond wounds, so that the wounds are bonded without using lines.

The present invention relates to a porous microsphere comprising a mussel adhesive protein and a method of preparing the same. The porous microsphere comprising the mussel adhesive protein according to the present invention is capable of minimally invasive bio-injection through syringes to efficiently deliver therapeutic stem cells to the sites of tissue defects as cell carriers. Further, the present invention may be widely applied to scaffolds for tissue engineering, drug carriers, or the like, which may be suitably applied to the size of the defected site of tissue.

The invention discloses a mussel-like coordination adhesive based on epsilon-polylysine and a preparation method of the mussel-like coordination adhesive. Key factors of a mussel adhesion environmentare introduced into the design of a biological adhesive; and the prepared adhesive has tissue adhesion performance and low cytotoxicity. The preparation method comprises the following steps of: firstly, uniformly mixing epsilon-polylysine with 3,4-dihydroxyphenylpropionic acid under an oxygen-free condition, then adding 1-(3-dimethylaminopropyl)-3-ethyl carbodiimide hydrochloride and N-hydroxysuccinimide into the mixture, and performing mixing and reacting to obtain the main component catechol modified epsilon-polylysine of the adhesive; dissolving PL-Cat in distilled water, adding Fe<3+> intothe distilled water, and adding NaOH to adjust the pH to be weakly alkaline, so that the PL-Cat/Fe adhesive can be obtained. The adhesive has good adhesive strength and low cytotoxicity, and has a wide application prospect in tissue engineering.

Provided are a surfactant adhesive protein comprising an amphiphilic peptide, as a surfactant adhesive protein, at the carbon or amine terminal, a silicone oil and an anticancer composition comprising the surfactant adhesive, where the surfactant adhesive enables homogeneous dispersion of hydrophilic or hydrophobic particles in a hydrophobic or hydrophilic solvent on the basis of strong adhesive strength of the mussel adhesive protein, and the surface adhesive can be favorably used as a surface coating agent requiring antibacterial or antiviral functions as well as a cosmetic product or an ink.

The invention discloses a method for improving evaluation efficiency of mussel adhesion resistance of a material. The method comprises the following steps: simulating a mussel adhesion process by virtue of a self-polymerization process of dopamine hydrochloride on the surface of the material; depositing polydopamine on the surface of the material to the maximum extent by regulating and controllingdopamine hydrochloride self-polymerization reaction conditions that are as follows: setting the pH value of the alkaline solution to be 8.5-10, the concentration of dopamine hydrochloride to be 15-21mmol/L, the concentration of the deposition promoter to be 50-200 mmol/L, the concentration of the deposition regulator to be 20-100 mmol/L, the reaction time to be 168 to 240 hours, the reaction temperature to be 20-30 DEG C; cleaning the sample after deposition; and evaluating the mussel fouling amount by adopting the deposition amount of the polydopamine film. The evaluation process does not need tedious microbial culture and the method is simple, convenient and feasible, has quantifiable evaluation results, can significantly improve the evaluation efficiency compared with a real sea coupon method, and is suitable for evaluating the mussel adhesion resistance of metal and non-metal materials.