Preparation method of a strong adhesive and high mechanical strength pegda-mussel adhesion protein-collagen composite hydrogel

A mussel adhesion protein and composite hydrogel technology, which is applied in the field of preparation of PEGDA-mussel adhesion protein-collagen composite hydrogel, can solve the problem of poor adhesion, limited application, unfavorable fixation, etc. of composite colloidal tissues problems to achieve good biosecurity results

Active Publication Date: 2022-06-03
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Collagen is also added to PEGDA gel by physical mixing to enhance cell adhesion, but after the two are physically mixed, the mechanical strength does not change significantly, and its mechanical strength is still not high
In addition, the tissue adhesion of the composite colloid is still poor, which is not conducive to the fixation on the tissue surface, limiting its application

Method used

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  • Preparation method of a strong adhesive and high mechanical strength pegda-mussel adhesion protein-collagen composite hydrogel
  • Preparation method of a strong adhesive and high mechanical strength pegda-mussel adhesion protein-collagen composite hydrogel
  • Preparation method of a strong adhesive and high mechanical strength pegda-mussel adhesion protein-collagen composite hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: Preparation method of PEGDA-mussel adhesion protein-collagen composite hydrogel.

[0032] 1) Dissolving mussel adhesion protein in an acetic acid solution with a volume concentration of 5%, stirring at 4°C for 5 minutes to obtain a mussel adhesion protein acetic acid solution, wherein the mussel adhesion protein mass volume concentration is 0.01g / mL;

[0033] 2) directly dissolve the solid fish skin collagen in the mussel adhesion protein acetic acid solution in step 1), and stir at 4°C for 4 hours to obtain the mussel adhesion protein-fish skin collagen solution, wherein the amount of fish skin collagen The volume concentration is 1mg / mL;

[0034] 3) Add 4M sodium hydroxide solution dropwise to the mussel adhesion protein-fish skin collagen solution obtained in step 2), and stir at 4°C to adjust the pH to 9;

[0035]4) Then, add the PEGDA solid into the mussel adhesion protein-fish skin collagen solution with a pH of 9, so that the mass volume concentration...

Embodiment 2

[0036] Example 2: Preparation method of PEGDA-mussel adhesion protein-collagen composite hydrogel.

[0037] 1) Dissolving mussel adhesion protein in an acetic acid solution with a volume concentration of 5%, stirring at 4°C for 5 minutes to obtain a mussel adhesion protein acetic acid solution, wherein the mussel adhesion protein mass volume concentration is 0.05g / mL;

[0038] 2) The solid fish skin collagen was directly dissolved in the mussel adhesion protein acetic acid solution, and stirred at 4°C for 4 hours to obtain the mussel adhesion protein-fish skin collagen solution, wherein the mass volume concentration of the fish skin collagen was 2 mg / mL;

[0039] 3) Add 4M sodium hydroxide solution dropwise to the mussel adhesion protein-fish skin collagen solution obtained in step 2), and stir at 4°C to adjust the pH to 7;

[0040] 4) Then, add the PEGDA solid into the mussel adhesion protein-fish skin collagen solution with a pH of 7, so that the mass volume concentration ...

Embodiment 3

[0041] Example 3: Preparation method of PEGDA-mussel adhesion protein-collagen composite hydrogel.

[0042] 1) Dissolving mussel adhesion protein in an acetic acid solution with a volume concentration of 5%, stirring at 4°C for 5 minutes to obtain a mussel adhesion protein acetic acid solution, wherein the mussel adhesion protein mass volume concentration is 0.1 g / mL;

[0043] 2) directly dissolving the methacrylamide collagen solid in the mussel adhesion protein acetic acid solution in step 1), and stirring at 4° C. for 4 hours to obtain the mussel adhesion protein-methacrylamide collagen protein solution, wherein formazan The mass volume concentration of acrylamide collagen is 4mg / mL;

[0044] 3) Add 4M sodium hydroxide solution dropwise to the mussel adhesion protein-methacrylamide collagen solution obtained in step 2), and stir at 4°C to adjust the pH to 5;

[0045] 4) Then, add the PEGDA solid into the mussel adhesion protein-methacrylamide collagen solution with a pH of...

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Abstract

The invention discloses a method for preparing a PEGDA-mussel adhesion protein-collagen composite hydrogel with strong adhesion and high mechanical strength, belonging to the field of hydrogel technology. In order to improve the mechanical strength and tissue adhesion of collagen hydrogel, the invention provides a kind of preparation method of strong adhesion, high mechanical strength PEGDA mussel adhesion protein collagen composite hydrogel, the The method is to dissolve mussel adhesion protein in acetic acid solution to obtain mussel adhesion protein acetic acid solution, dissolve the collagen solid in mussel adhesion protein acetic acid solution to obtain mussel adhesion protein-collagen solution, and neutralize with alkali pH to 5‑9, add PEGDA solid until completely dissolved, add photoinitiation factor, ultraviolet light crosslinking to form PEGDA‑mussel adhesion protein‑collagen composite hydrogel. The preparation process of the invention is simple and easy to operate, and has broad application prospects in the fields of tissue engineering and medicine.

Description

technical field [0001] The invention belongs to the technical field of hydrogels, and in particular relates to a preparation method of a PEGDA-mussel adhesion protein-collagen composite hydrogel with strong adhesion and high mechanical strength. Background technique [0002] Collagen is the main component of the extracellular matrix. It has the characteristics of biocompatibility, biodegradability, cell adhesion, and low immunogenicity. It has broad applications in drug delivery, skin substitutes, and tissue engineering. Currently commercialized collagen is mainly derived from pigskin and bovine Achilles tendon, etc., but the high extraction cost and the possibility of virus transmission limit the application of mammalian collagen. Fish skin and other aquatic product processing waste is one of the sources of collagen, with low price and high safety, so fish collagen gradually occupies a place in the market application. However, no matter whether collagen derived from mammal...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J3/28C08J3/24C08J3/075C08L89/00C08L71/02
CPCC08J3/28C08J3/246C08J3/075C08J2389/00C08J2371/02C08J2489/00C08J2471/02
Inventor 包子娴孙粤年锐咸漠樊喜英陈泉刘文帅彭馨莹
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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